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CUL4 E3 ligase regulates the proliferation and apoptosis of lung squamous cell carcinoma and small cell lung carcinoma

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Cancer Biology & Medicine 2020年第2期17卷 357-370页

作者： Ting Li si wu Lei Jia Wenfeng Cao yuan Yao Gang Zhao Hui Li Department of Gastrointestinal Cancer Biology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China Key Laboratory of Cancer Immunology and Biotherapy Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China Department of Pathology Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjinTianjin's Clinical Research Center for CancerTianjin 300060China

Objective:The E3 ligase,CRL4,plays diverse roles in different cellular processes,such as DNA damage,transcriptional regulation,cell cycle progression,and cell *** previous study showed that CUL4A and CUL4B had a strong association with tobacco smoking risk in lung squamous cell carcinoma(SCC)and small cell lung carcinoma(SCLC).This study aimed to define the potential mechanism underlying the roles of CUL4A and CUL4B in the development of SCC and ***:We determined the role of CUL4A and CUL4B in the cell cycle and apoptosis of SCC and SCLC,and identified the key apoptosis-related gene involved in the oncogenic activity of CUL4B by Western blot,immunohistochemical staining,flow cytometry,and enzyme inhibition ***:We found that depletion of CUL4A and CUL4B reduced the proliferation of SCC and SCLC ***4Aknockdown but not CUL4Bknockdown arrested cells in Gl phase while upregulating P21 and cU L4Bknockdown promoted cell apoptosis through upregulation o f ***,CUL4B decreased FO X03A expression by activating the ERK signaling pathway and mediating FOXO3A degradation via the ubiquitin-proteasome ***:These results identified the function of E3 ligase CRL4 in regulating SCC and SCLC cell proliferation,which provides a potential strategy for cancer therapy by targeting FOXO3A and the E3 ligase,CRL4.

关键词： Squamous cell lung cancer small cell lung cancer CUL4A CUL4B P21 FOXO3A

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In vitro antitumor effect of cucurbitacin E on human lung cancer cell line and its molecular mechanism

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Chinese Journal of Natural Medicines 2020年第7期18卷 483-490页

作者： JING si-yuan wu Zi-Dan ZHANG Tie-Hua ZHANG Jie WEI Zheng-Yi College of Food Science and Engineering Jilin UniversityChangchun 130062China Institute of Agricultural Biotechnology Jilin Academy of Agricultural SciencesChangchun 130033China

Cucurbitacin E(CuE) is previously reported to exhibit antitumor effect by several *** this study, CuE acted as a tyrosine kinase inhibitor interfering with the epidermal growth factor receptor/mitogen-activated protein kinase(EGFR/MAPK) signaling pathway and subsequently induced apoptosis and cell cycle arrest in non-small-cell lung cancer(NSCLC) cell line *** apoptosis regulators, cleaved Caspases-3 and Caspases-9, were observed to be increased with the treatment of *** activated transcription factor STAT3 and the apoptosis inhibitor protein survivin were also observed to be *** cell cycle regulators, CyclinA2, cylinB1, CyclinD1 and CyclinE, were also investigated and the results suggested that the cell cycle was arrested at G1/G0 *** of CuE also altered the existence status of most of the participants in the EGFR/MAPK *** of EGFR enhanced significantly, leading to the alteration of members downstream, either total amount or phosphorylation level, notably,MEK1/2 and ERK1/***, the results of molecular simulation brought an insight on the interaction mechanism between CuE and *** summary, CuE exhibited anti-proliferative effect against A549 cells by targeting the EGFR/MAPK signaling pathway.

关键词： Cucurbitacin E Antitumor effect Lung cancer cell Apoptosis Cell cycle arrest

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OFDM Based Bidirectional Multi-Relay SWIPT Strategy for 6G IoT Networks

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China Communications 2020年第12期17卷 80-91页

作者： Weidang Lu Peiyuan si Xin Liu Bo Li Zilong Liu Nan Zhao yuan wu Zhejiang University of Technology Hangzhou 310014China Dalian University of Technology Dalian 116024China Harbin Institute of Technology Weihai264209China University of Essex ColchesterCO43SQUnited Kingdom University of Macao Macao SAR 999078China

6G IoT networks aim for providing significantly higher data rates and extremely lower ***,due to the increasingly scarce spectrum bands and ever-growing massive number IoT devices(IoDs)deployed,6G IoT networks face two critical challenges,i.e.,energy limitation and severe signal *** wireless information and power transfer(SWIPT)and cooperative relaying provide effective ways to address these two *** this paper,we investigate the energy self-sustainability(ESS)of 6G IoT network and propose an OFDM based bidirectional multi-relay SWIPT strategy for 6G IoT *** the proposed strategy,the transmission process is equally divided into two ***,in phase1 two source nodes transmit their signals to relay nodes which will then use different subcarrier sets to decode information and harvest energy,*** phase2 relay nodes forward signals to corresponding destination nodes with the harvested *** maximize the weighted sum transmission rate by optimizing subcarriers and power *** proposed strategy achieves larger weighted sum transmission rate comparing with the benchmark scheme.

关键词： 6G IoT networks SWIPT multi-relay bidirectional communication OFDM

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Vertical gradients of neutral winds observed by ICON and estimated by the Horizontal Wind Model during the geomagnetic storm on August 26−28,2021

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Earth and Planetary Physics 2025年第1期9卷 69-80页

作者： JiaWei wu Chao Xiong YuYang Huang YunLiang Zhou Department of Space Physics School of Electronic InformationHubei Luojia LaboratoryWuhan UniversityWuhan 430072China

The Michelson Interferometer for Global High-resolution Thermospheric Imaging(MIGHTI)onboard the Ionospheric Connection Explorer(ICON)satellite offers the opportunity to investigate the altitude profile of thermospheric *** this study,we used the red-line measurements of MIGHTI to compare with the results estimated by Horizontal Wind Model 14(HWM14).The data selected included both the geomagnetic quiet period(December 2019 to August 2022)and the geomagnetic storm on August 26-28,*** the geomagnetic quiet period,the estimations of neutral winds from HWM14 showed relatively good agreement with the observations from *** to the ICON observations,near the equator,zonal winds reverse from westward to eastward at around 06:00 local time(LT)at higher altitudes,and the stronger westward winds appear at later LTs at lower *** around 16:00 LT,eastward winds at 300 km reverse to westward,and vertical gradients of zonal winds similar to those at sunrise hours can be *** the middle latitudes,zonal winds reverse about 2-4 h *** winds vary more significantly than zonal winds with seasonal and latitudinal *** to the ICON observations,in the northern low latitudes,vertical reversals of meridional winds are found at 08:00-13:00 LT from 300 to 160 km and at around 18:00 LT from 300 to 200 km during the June *** reversals of meridional winds are found at 04:00-07:00 LT from 300 to 160 km and at 22:00-02:00 LT from 270 to 200 km during the December *** the southern low latitudes,meridional wind reversals occur at 08:00-11:00 LT from 200 to 160 km and at 21:00-02:00 LT from 300 to 200 km during the June *** the December solstice,reversals of the meridional wind appear at 20:00-01:00 LT below 200 km and at 06:00-11:00 LT from 300 to 160 *** the northern middle latitudes,the northward winds are dominant at 08:00-14:00 LT at 230 km during the June *** winds persist until

关键词： horizontal neutral winds vertical gradients Ionospheric Connection Explorer satellite Horizontal Wind Model 14 geomagnetic storm

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Harnessing the Streptomyces-originating type I-E CRISPR/Cas system for efficient genome editing in Streptomyces

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Science China Life Sciences 2025年

作者： Yuhui Xie Xiaoyan Liu Tingting wu Yunzi Luo Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education) School of Chemical Engineering and TechnologyTianjin University Department of Gastroenterology State Key Laboratory of BiotherapyWest China HospitalSichuan University Georgia Tech Shenzhen Institute Tianjin University Haihe Laboratory of Sustainable Chemical Transformations

since their discovery,CRISPR/Cas systems have significantly expanded the genetic toolbox,aiding in the exploration and enhanced production of natural products across various *** these,class 2 CRISPR/Cas systems are simpler and more broadly used,but they frequently fail to function effectively in many Streptomyces *** this study,we present an engineered class 1 type I CRISPR/Cas system derived from Streptomyces avermitilis,which enables efficient gene editing in phylogenetically distant Streptomyces *** a plasmid interference assay,we identified the effective protospacer adjacent motif as 5′-AAN-3′.Utilizing this system,we achieved targeted chromosomal deletions ranging from 8 bp to 100 kb,with efficiencies exceeding 92%.We further utilized this system to insert DNA fragments into different Streptomyces genomes,facilitating the heterologous expression of exogenous genes and the activation of endogenous natural product biosynthetic gene ***,we established a type I CRISPR/Cas-based gene-editing methodology that significantly advances the exploration of Streptomyces,known for their rich natural product *** is the first report of a gene editing tool developed based on the endogenous class 1 type I CRISPR/Cas system in Streptomyces *** work enriches the Streptomyces gene manipulation toolbox and advances the discovery of valuable natural products within these organisms.

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Effects of aggregate size distribution and carbon nanotubes on the mechanical properties of cemented gangue backfill samples under true triaxial compression

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International Journal of Minerals,Metallurgy and Materials 2025年第2期32卷 311-324页

作者： Qian Yin Fan Wen Zhigang Tao Hai Pu Tianci Deng Yaoyao Meng Qingbin Meng Hongwen Jing Bo Meng Jiangyu wu State Key Laboratory of Intelligent Construction and Healthy Operation and Maintenance of Deep Underground Engineering China University of Mining and TechnologyXuzhou 221116China State Key Laboratory of Mining Response and Disaster Prevention and Control in Deep Coal Mines Anhui University of Science and TechnologyHuainan 232001China State Key Laboratory for Geomechanics&Deep Underground Engineering China University of Mining and Technology(Beijing)Beijing 100083China Key Laboratory of Xinjiang Coal Resources Green Mining Ministry of EducationXinjiang Institute of EngineeringUrumqi 830023China

The mechanical behavior of cemented gangue backfill materials(CGBMs)is closely related to particle size distribution(PSD)of aggregates and properties of cementitious ***,the true triaxial compression tests,CT scanning,SEM,and EDS tests were conducted on cemented gangue backfill samples(CGBSs)with various carbon nanotube concentrations(P_(CNT))that satisfied fractal theory for the PSD of *** mechanical properties,energy dissipations,and failure mechanisms of the CGBSs under true triaxial compression were systematically *** results indicate that appropriate carbon nanotubes(CNTs)effectively enhance the mechanical properties and energy dissipations of CGBSs through micropore filling and microcrack bridging,and the optimal effect appears at P_(CNT)of 0.08wt%.Taking PSD fractal dimension(D)of 2.500 as an example,compared to that of CGBS without CNT,the peak strength(σ_(p)),axial peak strain(ε_(1,p)),elastic strain energy(Ue),and dissipated energy(U_(d))increased by 12.76%,29.60%,19.05%,and90.39%,***,excessive CNTs can reduce the mechanical properties of CGBSs due to CNT agglomeration,manifesting a decrease inρ_(p),ε_(1,p),and the volumetric strain increment(Δε_(v))when P_(CNT)increases from 0.08wt%to 0.12wt%.Moreover,the addition of CNTs improved the integrity of CGBS after macroscopic failure,and crack extension in CGBSs appeared in two modes:detour and pass through the ***σ_(p)and U_(d)firstly increase and then decrease with increasing D,and porosity shows the opposite ***ε_(1,p)andΔε_(v)are negatively correlated with D,and CGBS with D=2.150 has the maximum deformation parameters(ε_(1,p)=0.05079,Δε_(v)=0.01990)due to the frictional slip effect caused by coarse *** increasing D,the failure modes of CGBSs are sequentially manifested as oblique shear failure,"Y-shaped"shear failure,and conjugate shear failure.

关键词： cemented gangue backfill materials particle size distribution true triaxial compression test carbon nanotubes mechanical properties failure modes

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Grounded-source short offset transient electromagnetic method:Theory and applications in deep mineral exploration

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Science China Earth Sciences 2025年

作者： Guoqiang XUE Xin wu Weiying CHEN Nannan ZHOU Key Laboratory of Mineral Resources Institute of Geology and Geophysics Chinese Academy of Sciences College of Earth and Planetary Sciences University of Chinese Academy of Sciences

Abstract The Transient Electromagnetic (TEM) method is a critical geophysical technique for subsurface exploration of metal ore bodies, primarily utilizing either loop or grounded transmitters. The Long Offset Transient Electromagnetic(LOTEM) method employs a grounded-source transmitter, relying on a far-source observation mode and plane wave approximation for detection. However, LOTEM’s far-source configuration weakens signal strength, and the plane wave approximation reduces precision, limiting effective detection depth to approximately 1000 m with a comprehensive error of about 15%. Recently, we have developed the grounded-source Short Offset Transient Electromagnetic (SOTEM) method,achieving greater detection depth and accuracy within the 500–2000 m depth range, a crucial interval for mineral resource exploration. This study explores the theoretical framework, instrumentation, data processing, and field applications of SOTEM. Based on a point charge element model, SOTEM accurately computes surface wave effects in EM field calculations,optimized for near-source observation. High-power, high-resolution, wide-bandwidth exploration equipment and an advanced three-dimensional hybrid inversion technique were also developed to enhance the method’s effectiveness. Application of SOTEM to the deep exploration of the Zhou’an Ni-Cu-PGE deposit in Henan Province yielded high-resolution imaging of conductivity structures to about 2.5 km depth. These results, consistent with existing drill data, delineated mineralized ore bodies from surrounding formations, identified zones of mineralization potential, and suggested extensive resource prospects in the region.

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High-dense nanocrystalline W-based alloy prepared by hot isostatic pressing with uniform microstructure, ultrahigh hardness and good thermal stability

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Tungsten 2025年第1期7卷 120-136页

作者： Zao-Ming wu Lin He Lin-Sen Ye Xiao-Feng Yang Jia-Jia Zhang Hong-Bo Lei Institute of Materials China Academy of Engineering Physics

High-density uniform microstructure nanocrystalline W-10wt.% Cr-5wt.% Y（W-10Cr-5Y） alloy with an average grain size of 85 nm and a relative density of 99.8% was successfully prepared.W-Cr-Y alloys with different amounts of second-phase particles were prepared by high-energy ball milling and hot isostatic pressing *** effects of composition design,ball milling parameters,and sintering parameters on the microstructure of W-Cr-Y alloys were studied.A feasible technology for controlling the synthesis of high-density nanocrystalline W-based alloys has been *** microstructure analysis of nanocrystalline W-10Cr-5Y alloy shows that there are two types of doping in the second phase:intergranular doping and intragranular *** "uniform distribution YOx-rich particles" effectively suppresses the grain growth of W and leads to the formation of nanocrystalline W-10Cr-5Y alloy.W-10Cr-5Y has an ultra-high hardness,with a Vickers hardness of 1312±12 kgf·***-temperature annealing treatment shows that W-10Cr-5Y has good thermal stability at 1000 and1100℃,as demonstrated by the comparison of Vickers hardness and grain size between annealed and unannealed alloys.

关键词： Hot isostatic pressing Microstructure Nanocrystalline tungsten Thermal stability Vickers hardness

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simplified Numerical Models of the Unsteady Tip Leakage Flow in Compressor

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Journal of Thermal Science 2023年第6期32卷 2386-2399页

作者： si Changxin wu Zihao LIU Xiaohua School of Aeronautics and Astronautics Shanghai Jiao Tong UniversityShanghai200240China China Three Gorges Investment Management Co. Ltd.Shanghai200124China Key Laboratory of Aerodynamic Noise Control China Aerodynamics Research and Development CenterMianyang621000China

Tip leakage flow(TLF)in compressors,which can cause flow blockage in blade passage and induce efficiency loss,is also a potential threat to the unsteady flow stability of modern *** paper provides an overview of the significance of tip leakage flow research,and introduces relevant previous *** calculating by different methods,large eddy simulation(LES)is demonstrated again as a suitable compromise between accuracy and computational cost for the unsteady flow *** types of simplified tip leakage flow models using LES are adopted with a focus on the unsteady characteristics of shedding vortices in a cavity *** paper applies these models to study the unsteady tip leakage flow which triggers the onset of acoustic resonance in a multistage axial *** with the detected acoustic resonance frequency of 5.22 rotational frequency(RF)in the previous experiment,the computed combination frequency in the 2-D model is equal to 5.232RF,and the simplified 3-D unsteady tip leakage flow model results in a combination frequency of ***,based on the small relative error between model results and experimental results,the simplified numerical models are validated to be sufficiently accurate,and theoretically provide a useful basis for the subsequent research of unsteady tip leakage flow in turbomachinery.

关键词： turbomachinery compressor tip leakage flow unsteady flow

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Discrimination of pp solar neutrinos and^(14)C double pile-up events in a large-scale LS detector

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Nuclear Science and Techniques 2023年第9期34卷 69-81页

作者： Guo-Ming Chen Xin Zhang Ze-yuan Yu si-yuan Zhang Yu Xu Wen-Jie wu Yao-Guang Wang Yong-Bo Huang School of Physical Science and Technology Guangxi UniversityNanning 530004China Institute of High Energy Physics Beijing 100049China University of Chinese Academy of Sciences Beijing 100049China School of Physics Sun Yat-Sen UniversityGuangzhou 510275China Department of Physics and Astronomy University of CaliforniaIrvineCAUSA

As a unique probe,the precision measurement of pp solar neutrinos is important for studying the sun’s energy mechanism as it enables monitoring the thermodynamic equilibrium and studying neutrino oscillations in the vacuum-dominated *** a large-scale liquid scintillator detector,a bottleneck for pp solar neutrino detection is the pile-up events of intrinsic14C *** paper presents a few approaches to discriminating between pp solar neutrinos and ^(14)C pile-up events by considering the differences in their time and spatial *** this study,a Geant4-based Monte Carlo simulation is *** analysis and deep learning technology are adopted to investigate the capability of ^(14)C pile-up *** BDTG (boosted decision trees with gradient boosting) model and VGG network demonstrate good performance in discriminating pp solar neutrinos and ^(14)C double pile-up *** the ^(14)C concentration assumption of 5×10-18g/g,the signal significance can achieve 10.3 and 15.6 using the statistics of only one *** this case,the signal efficiency for discrimination using the BDTG model while rejecting 99.18% ^(14)C double pile-up events is 51.1%,and that for the case where the VGG network is used while rejecting 99.81%of the ^(14)C double pile-up events is 42.7%.

关键词： Liquid scintillator detector pp solar neutrinos 14C pile-up Multivariate analysis Deep learning

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