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Journal of Hainan Medical University 2018年第23期24卷 63-66页

作者： Ding-An Li si-Yu Chen Hong-Ni Li Department of Neurology No.1WardHanzhong Central Hospital in Shaanxi ProvinceHanzhongShaanxi Province723000China Department of Neurology the Fourth Military Medical UniversityXi'anShaanxi Province71000China Department of Neurology Xi'an TCM Brain Disease Hospital in Shaanxi ProvinceXi'anShaanxi Province710032China

Objective:To study the changes of CD4+CD25+ and CD4+CD28-T cells in peripheral blood of patients with stroke and their correlation with neuronal damage markers, inflammatory cytokines and plaque stability ***: The patients who were diagnosed with acute ischemic stroke in our hospital between June 2014 and December 2017 were selected as the stroke group of the research, and healthy volunteers who received physical examination during the same period were selected as the control group. Peripheral blood was collected to determine the contents of CD4+CD25+ and CD4+CD28-T cells, and serum was collected to determine the contents of neuron damage markers, inflammatory cytokines and plaque stability ***: Peripheral blood CD4+CD25+ cell content as well as serum BDNF, IGF-1, IL-10, TGF-β1, TIMP2 and Vaspin contents of stroke group was lower than those of control group whereas peripheral blood CD4+CD28-T cell content as well as serum NSE, VILIP-1, ET-1, IL-6, CXCL12, VCAM-1, P-selectin, ox-LDL, CatS, ICTP and VEGF contents was higher than those of control group;peripheral blood CD4+CD25+ cell content of stroke group was positively correlated with serum BDNF, IGF-1, IL-10, TGF-β1, TIMP2 and Vaspin contents, and negatively correlated with NSE, VILIP-1, ET-1, IL-6, CXCL12, VCAM-1, P-selectin, ox-LDL, CatS, ICTP and VEGF contents;peripheral blood CD4+CD28-T cell content was negatively correlated with serum BDNF, IGF-1, IL-10, TGF-β1, TIMP2 and Vaspin contents, and positively correlated with NSE, VILIP-1, ET-1, IL-6, CXCL12, VCAM-1, P-selectin, ox-LDL, CatS, ICTP and VEGF ***: The changes of CD4+CD25+ and CD4+CD28-T cells in peripheral blood of patients with stroke can aggravate the neuron damage and promote the inflammatory response activation and plaque stability decline.

关键词： Stroke CD4+CD25+ T cell CD4+CD28-T cell Inflammatory response Plaque properties

ALKBH1 deficiency leads to loss of homeostasis in human diploid somatic cells

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Protein & Cell 2020年第9期11卷 688-695页

作者： Hongyu Li Zeming Wu Xiaoqian Liu Sheng Zhang Qianzhao Ji Xiaoyu Jiang Zunpeng Liu si Wang Jing Qu Weiqi Zhang Moshi Song Eli Song Guang-Hui Liu National Laboratory of Biomacromolecules CAS Center for Excellence in BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China State Key Laboratory of Stem Cell and Reproductive Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China State Key Laboratory of Membrane Biology Institute of ZoologyChinese Academy of SciencesBeijing 100101China Institute for Stem Cell and Regeneration Chinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China Advanced Innovation Center for Human Brain Protection National Clinical Research Center for Geriatric DisordersXuanwu Hospital Capital Medical UniversityBeijing 100053China

Dear Editor,As the most prevalent DNA methylation modification in prokaryotes,DNA N6 methyladenosine(6mA)in eukaryotic genomes has recently been observed in diverse species including Caenorhabditis elegans(Greer et al.,2015),Dro-sophila melanogaster(Zhang et al,2015),mouse(Wu et al,2016)and human(Xiao et al,2018).6mA has been reported to associate with multiple physiological processes including embryonic development and tumorigenesis(Greer et al.,2015;Zhang et al.,2015;Xie et al.,2018),yet some con-troversies *** contrast to the findings showing that ALKBH1(alkB homolog 1)is a primary 6mA demethylase in mouse and human cells(Wu et al.,2016;Xiao et al.,2018;Xie et al..2018),other studies indicate that ALKBH1 is prone to demethylate 6mA on bubbled or bulged DNAs that are often featured by a locally unpairing region with flanking duplex,such as D-loop,R-loop as well as DNA or RNA stem-loop,and single-stranded DNAs at a lower efficiency.

关键词： homeostasis bubble pairing

NHC-Au（Ⅰ）-catalyzed synthesis of benzo[b][1,4]diazepines from N-substituted o-phenylenediamines and terminal alkynes

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Weekly albumin-bound paclitaxel/cisplatin versus gemcitabine/cisplatin as first-line therapy for patients with advanced non-small-cell lung cancer:A phase II open-label clinical study

第十七届全国金属有机化学学术讨论会

作者： Pei Guo Xiaoming Zeng si Chen Meiming Luo College of Chemistry Sichuan University Frontier Institute of Science and Technology Xi′an Jiaotong University

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关键词：

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Chinese Journal of Cancer Research 2019年第2期31卷 339-348页

作者： Shanshan Qin Hui Yu Xianghua Wu Zhiguo Luo Huijie Wang si Sun Mingzhu Huang Jia Jin Zhonghua Tao Jie Qiao Yu Feng Jialei Wang Jianhua Chang Department of Medical Oncology Fudan University Shanghai Cancer CenterDepartment of Oncology Shanghai Medical College Fudan University

Objective: The aim of this trial was to compare both the efficacy and the safety of a weekly nanoparticle albumin-bound paclitaxel(nab-paclitaxel) plus cisplatin vs. gemcitabine plus cisplatin in patients with advanced non-small-cell lung cancer(NSCLC).Methods: A total of 84 participants received either 100 mg/m^2 nab-paclitaxel each week on d 1, 8 and 15 of a 28 day cycle, as well as cisplatin 75 mg/m^2 on d 1 every three weeks(nab-TP arm); or gemcitabine 1,000 mg/m^2 on d 1 and 8, plus cisplatin 75 mg/m^2 on d 1 every three weeks(GP arm). The primary end point was progression-free survival(PFS). The secondary end points were overall response rate(ORR) and overall survival(OS).Results: According to our analysis, the median PFS was 4.8 months for the nab-TP arm vs. 5.2 months for the GP arm(P=0.55). Analysis showed the median OS was 14.6 months for participants who were in the nab-TP arm vs. 15.1 months for those in the GP arm(P=0.94). Besides, nab-TP showed OS advantages over GP in patients harboring epidermal growth factor receptor(EGFR) mutation(26.7 vs. 15.3 months, P=0.046) and patients with a performance status of 0(23.5 vs. 14.7 months, P=0.020). It was found that incidences of drug-related grade 3 or 4 toxicities were comparable between the two treatment ***: Therefore, it can be seen that weekly nab-TP treatment has a similar efficacy and tolerability to GP treatment for patients who are undergoing their first-line treatment for NSCLC. It could be that survival differences among platinum doublets in the context of both EGFR mutation and performance status have the potential to be the basis for our further clinical trials.

关键词： Albumin-bound paclitaxel cisplatin gemcitabine first-line therapy advanced non-small-cell lung cancer

Large Gravity Flow Deposits in the Member 1 of Paleogene Shahejie Formation, Qikou Sag, Bohai Bay Basin

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Large Gravity Flow Deposits in the Member 1 of Paleogene Sha...

人生长分化因子11基因原核表达质粒的构建、表达及其蛋白的纯化

第十五届全国古地理学及沉积学学术会议

作者： si Chen Hua Wang Chuanyan Huang Tianhao Gong Key Laboratory of Tectonics and Petroleum Resources MOEChina University of Geosciences

The large scale gravity flow deposits（up to 1700 km2） that developed in the Member 1 of Shahejie Formation（Paleogene） in the Binhai slope area in the Qikou Sag shows the sedimentary characters of multiple sediment sources, long distance and multi-fault transport, and preservation along the transfer path during the sedimentary processes. This study focus on the gravity flow deposits in the oil-rich Qikou Sag in Bohai Bay Basin. Under the sequence stratigraphy framework, the source-transport path-sink processes has been discussed. The aim of this study is to（1） illuminate the formation of the multiple sources, the configuration relationship between different sources, and the origin of continuous sediment supply.（2） analyze the controlling and evolution of the paleogeomorphology（fault-controlling steep slope and multi-step fault slope）, sediment transport, the sedimentary develop mechanism in processes.（3） focus on the distributions, sedimentary patterns and space evolution of the large scale multi-stages gravity flow deposits.（4） discuss the formation mechanism and controlling factors of the gravity flow in Qikou Sag. The conclusions include:（1） the gravity flow was controlled by multiple sources. During the Member 1 of Shahejie Formation, the Binhai slope was supplied by sources from four different areas that include: Dashentang source from northern Yanshan Mountain, Chadian sources, Gegu and Xiaozhan sources from western Cangxian Uplift. The continuous sediment supply allows the large scale gravity flow to be formed in the lacustrine basin.（2） the paleogeomorphology of the Member 1 of Shahejie Formation in the Qikou Sag is the complex of fault-controlling steep slope and multi-step fault slope. The fault-controlling steep slope is developed in the northern part of Binhai Slope, as the multi-step fault slope is mainly developed in the orientation of west to east in the west part of Binhai Slope. The fault zones play a role as the transport paths for the sediments and

关键词： Qikou Sag Gravity flow deposits multiple overlap layers thin alternating beds high quality reservoir

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中国生物制品学杂志 2018年第7期31卷 723-727页

作者：吴思思蒋维四川大学华西医院公共实验技术中心四川成都610041

目的构建及表达人生长分化因子11(growth differentiation factor 11,GDF11)基因原核表达质粒,并对重组表达蛋白进行纯化。方法 PCR法扩增人源GDF11 cDNA全长序列,插入至载体pCold-I,构建重组质粒pCold-GDF11,转化感受态Rosetta(DE3)菌... 详细信息

目的构建及表达人生长分化因子11(growth differentiation factor 11,GDF11)基因原核表达质粒,并对重组表达蛋白进行纯化。方法 PCR法扩增人源GDF11 cDNA全长序列,插入至载体pCold-I,构建重组质粒pCold-GDF11,转化感受态Rosetta(DE3)菌株,经终浓度为0.5 mmol/L的IPTG诱导表达后,超声破碎获得GDF11包涵体,添加8 mol/L尿素溶解,与Ni^(2+)NTA Agarose充分混匀后装柱,用含谷胱甘肽(glutathione,GSH)和氧化型谷胱甘肽(oxidized glutathione,GSSG)的复性液进行复性,经含咪唑的洗脱液洗脱获得目的蛋白,进行Western blot检测。结果重组质粒pCold-GDF11经双酶切及测序鉴定,构建正确;诱导表达蛋白的相对分子质量约45 000,主要以包涵体形式存在;GDF11蛋白纯化产物纯度达85%以上,每升菌液获得2.5 mg的目的蛋白,且可与小鼠抗His单克隆抗体发生特异性结合。结论成功构建了GDF11基因原核表达质粒,且表达产物经纯化后可获得纯度较高的目的蛋白。

关键词：人生长分化因子11 原核表达复性纯化

His bundle pacing versus left bundle branch pacing on ventricular function in atrial fibrillation patients referred for pacing:a prospective crossover comparison

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Journal of Geriatric Cardiology 2023年第1期20卷 51-60页

作者： Yang YE Bo GAO Yuan LV Tian-Tian XU si-si ZHANG Xiao-Li LU Ying YANG Dong-Mei JIANG Yi-Wen PAN Xia SHENG Bei WANG Yan-Kai MAO Zu-Wen ZHANG Shi-Quan CHEN Jie-Fang ZHANG Li WANG Jiang-Fen JIANG Ya-Xun SUN Yan MA Fei-Ling WANG Min WANG Hong HE Chen-Yang JIANG Guo-Sheng FU Department of Cardiology Sir Run Run Shaw HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina Department of Cardiology Zhejiang Rongjun HospitalJiaxingZhejiang ProvinceChina Department of Cardiology Lishui People’s HospitalLishuiZhejiang ProvinceChina Department of Cardiology Ningbo Ninth HospitalNingboZhejiang ProvinceChina Department of Cardiology Anji people’s HospitalNingboZhejiang ProvinceChina Department of Diagnostic Ultrasound and Echocardiography Sir Run Run Shaw HospitalCollege of MedicineZhejiang UniversityHangzhouZhejiang ProvinceChina

BACKGROUND His bundle pacing(HBP)and left bundle branch pacing(LBBP)both provide physiologic pacing which maintain left ventricular *** both improve heart failure(HF)symptoms in atrial fibrillation(AF)*** aimed to assess the intra-patient comparison of ventricular function and remodeling as well as leads parameters corresponding to two pacing modalities in AF patients referred for pacing in intermediate *** Uncontrolled tachycardia AF patients with both leads implantation successfully were randomized to either *** measurements,New York Heart Association(NYHA)classification,quality-of-life assessments and leads parameters were obtained at baseline and at each 6-month follow *** ventricular function including the left ventricular endosystolic volume(LVESV),left ventricular ejection fraction(LVEF)and right ventricular(RV)function quantified by tricuspid annular plane systolic excursion(TAPSE)were all *** Consecutively twenty-eight patients implanted with both HBP and LBBP leads successfully were enrolled(69.1±8.1 years,53.6% male,LVEF 59.2%±13.7%).The LVESV was improved by both pacing modalities in all patients(n=23)and the LVEF was improved in patients with baseline LVEF at less than 50%(n=6).The TAPSE was improved by HBP but not LBBP(n=23).CONCLUsiON In this crossover comparison between HBP and LBBP,LBBP was found to have an equivalent effect on LV function and remodeling but better and more stable parameters in AF patients with uncontrolled ventricular rates referred for atrioventricular node(AVN)*** could be preferred in patients with reduced TAPSE at baseline rather than LBBP.

关键词： ventricular patients bundle

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不同摆角下四墙切圆燃烧器的数值模拟

中国电机工程学报 2011年第11期31卷 17-24页

作者：李永华司金茹电站设备状态监测与控制教育部重点实验室(华北电力大学) 河北省保定市071003

四角切圆燃烧方式在电厂中的应用很广泛,但是这种燃烧方式也有其自身的缺点:如燃烧器区域温度水平低,着火条件差,炉膛出口残余旋转大,水平烟道温度分布不均匀,容易产生热偏差和水冷壁的爆管等。因此近几年来四墙切圆燃烧方式具有很好的... 详细信息

四角切圆燃烧方式在电厂中的应用很广泛,但是这种燃烧方式也有其自身的缺点:如燃烧器区域温度水平低,着火条件差,炉膛出口残余旋转大,水平烟道温度分布不均匀,容易产生热偏差和水冷壁的爆管等。因此近几年来四墙切圆燃烧方式具有很好的工程应用前景。以某电厂600 MW超超临界四墙切圆直流摆动式燃烧器为工程原型建立模型,通过FLUENT数值计算软件,来用数值模拟的方法研究直流摆动式燃烧器在上下不同摆角下的燃烧特性与流动特性,根据数值模拟的结果分析其燃烧特性和流动特性,确定了四墙切圆燃烧方式的合理工况,为四墙切圆燃烧锅炉的运行提供了参考。

关键词：四墙切圆不同摆角燃烧特性流动特性数值模拟

ACHIEVING BETTER UNDERSTANDING BY LISTENING WITH PREDICTION

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Chinese Journal of Applied Linguistics 2000年第4期23卷 34-38页

作者： si Jun Han Chunming University of Science and Technology of China

The skill of Listening with comprehension is anessential part of communication and basic to ESL/EFL *** is also a key process in un-derstanding spoken *** article intends tooffer a definition for prediction in ESL/EFL listening,examines its foundations,draws some insights into itsnature,and illustrates how to develop and employprediction in pre-listening and while-listening stages,also how to‘repair’prediction in the post-listeningstage,in an attempt to help listeners achieve the goalof better understanding.

关键词： ESL EFL ACHIEVING BETTER UNDERSTANDING BY LISTENING WITH PREDICTION