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Generation of selectable marker-free soft transgenic rice with transparent kernels by downregulation of SSSⅡ-2

The Crop Journal 2020年第1期8卷 53-61页

作者： Zhihao Xu Meimei Yu Youwei Yin Cenwen Zhu Wen Ji Changquan Zhang Qianfeng Li Honggen Zhang shuzu tang Hengxiu Yu Qiaoquan Liu Jiangsu Key Laboratory of Crop Genetics and Physiology Key Laboratory of Plant Functional Genomics of the Ministry of EducationJiangsu Key Laboratory of Crop Genomics and Molecular BreedingJiangsu Co-Innovation Center for Modern Production Technology of Grain CropsCollege of AgricultureYangzhou University

The amylose content(AC) of rice endosperm starch varies from 0 to 35%,and is associated with rice cooking and eating *** rice has low AC,generally between 6% and 15%,and its eating quality is high whether it is consumed hot or ***,the appearance quality of current soft rice cultivars needs to be improved,especially opaque *** genetic engineering has improved some agronomic traits of soft rice varieties,but not endosperm *** the present study,a RNAi construct of the soluble starch synthase Ⅱ-2(SSSⅡ-2) and the hygromycin phosphotransferase(HPT) gene were introduced into an elite japonica rice variety,Kangtiaowuyunjing(KWY8) by *** selectable marker-free(SMF) transgenic lines were obtained,and SSSⅡ-2 expression was significantly downregulated in selected transgenic lines,resulting in lower AC of the *** physicochemical properties of the transgenic rice kernels,including gel consistency(GC) and rapid visco analyzer(RVA) profile,differed significantly from those of wild-type rice and were similar to those of a soft rice variety,Nanjing 46(NJ46).These findings indicate that the cooking,eating,and processing qualities of transgenic rice are comparable to those of ***,the transgenic rice endosperm retained a transparent appearance under low-moisture ***,SMF SSSⅡ-2 RNAi rice provides a resource for breeding soft rice with transparent endosperm.

关键词： Rice(Oryza sativa L.) SSSⅡ-2 RNAi Starch quality SMF transgenic lines

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Generation of selectable marker-free soft transgenic rice with transparent grains through down-regulation SSSII-2 gene

Generation of selectable marker-free soft transgenic rice wi...

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江苏省遗传学会2019年学术研讨会

作者： Yan liu Zhihao Xu Maosen Xu Jiaqi tang Changquan Zhang Qianfeng Li Honggen Zhang shuzu tang Hengxiu Yu Qiaoquan Liu Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Jiangsu CoInnovation Center for Modern Production Technology of Grain Crops Agricultural College of Yangzhou University

Rice（Oryza sativa L.） provides food for more than half of the world’s population. The amylose content（AC） of rice endosperm starch varies from 0 to 35%, and is associated with rice cooking and eating quality. Soft rice has low AC, generally between 6% and 15%, and its eating quality is high whether it is consumed hot or cold. However, the appearance quality of current soft rice cultivars needs to be improved, especially opaque endosperm. Conventional genetic engineering has improved some agronomic traits of soft rice varieties, but not endosperm appearance. Amylose synthesis in rice is catalyzed mainly by granulebound starch synthase I（GBSSI）, encoded by the Waxy（Wx） gene. Amylopectin is catalyzed by soluble starch synthase（SSS）, starch branching enzyme（SBE）, and starch debranching enzyme（DBE）. SSS can be divided into four subfamilies（SSSI-IV） with eight isoforms（SSSI, SSSIIa, SSSIIb, SSSIIc, SSSIIIa, SSSIIIb, SSSIVa, and SSSIVb）. SSSIIb is also known as SSSII-2. In the present study, a RNAi construct of the soluble starch synthase II-2（SSSII-2） and the hygromycin phosphotransferase（HPT） gene were introduced into an elite japonica rice variety, Kangtiaowuyunjing（KWY8） by co-transformation. Several selectable markerfree（SMF） transgenic lines were obtained, and SSSII-2 expression was significantly downregulated in selected transgenic lines, resulting in lower AC of the endosperm. The physicochemical properties of the transgenic rice kernels, including gel consistency（GC） and rapid visco analyzer（RVA） profile, differed significantly from those of wild-type rice and were similar to those of a soft rice variety, Nanjing 46（NJ46）. These findings indicate that the cooking, eating, and processing qualities of transgenic rice are comparable to those of NJ46. However, the transgenic rice endosperm retained a transparent appearance under lowmoisture conditions. Thus, SMF SSSII-2 RNAi rice provides a resource for breeding soft rice with transparent endosperm.

关键词： rice(Oryza sativa) SSSII-2 RNAi starch quality transgenic lines

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Generation of selectable marker-free soft transgenic rice with transparent kernels by downregulation of SSSII-2

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The Crop Journal 2020年第1期

作者： Zhihao Xu Meimei Yu Youwei Yin Cenwen Zhu Wen Ji Changquan Zhang Qianfeng Li Honggen Zhang shuzu tang Hengxiu Yu Qiaoquan Liu Jiangsu Key Laboratory of Crop Genetics and Physiology，Key Laboratory of Plant Functional Genomics of the Ministry of Education，Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding，Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops，College of Agriculture，Yangzhou University

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