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C1QTNF5 is a novel attachment factor that facilitates the entry of influenza A virus

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Virologica Sinica 2024年第2期39卷 277-289页

作者： Lei Yu Xinjin Liu Xiaoqin Wei Junrui Ren Xueyun Wang shuwen wu Ke Lan State Key Laboratory of Virology Medical Research InstituteCollege of Life SciencesWuhan UniversityWuhan430072China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan430072China Taikang Center for Life and Medical Sciences Wuhan UniversityWuhan430072China

Influenza A virus(IAV)binds sialic acid receptors on the cell surface to enter the host cells,which is the key step in initiating infection,transmission and pathogenesis.Understanding the factors that contribute to the highly efficient entry of IAV into human cells will help elucidate the mechanism of viral entry and pathogenicity,and provide new targets for intervention.In the present study,we reported a novel membrane protein,C1QTNF5,which binds to the hemagglutinin protein of IAV and promotes IAV infection in vitro and in vivo.We found that the HA1 region of IAV hemagglutinin is critical for the interaction with C1QTNF5 protein,and C1QTNF5 interacts with hemagglutinin mainly through its N-terminus(1–103 aa).In addition,we further demonstrated that overexpression of C1QTNF5 promotes IAV entry,while blocking the interaction between C1QTNF5 and IAV hemagglutinin greatly inhibits viral entry.However,C1QTNF5 does not function as a receptor to mediate IAV infection in sialic acid-deficient CHO-Lec2 cells,but promotes IAV to attach to these cells,suggesting that C1QTNF5 is an important attachment factor for IAV.This work reveals C1QTNF5 as a novel IAV attachment factor and provides a new perspective for antiviral strategies.

关键词： C1QTNF5 Influenza A virus Viral entry Hemagglutinin Attachment factor

Advances in single-cell sequencing technology in microbiome research

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Genes & Diseases 2024年第4期11卷 201-219页

作者： Yinhang wu Jing Zhuang Yifei Song Xinyi Gao Jian Chu shuwen Han Huzhou Central Hospital Affiliated Central Hospital Huzhou UniversityHuzhouZhejiang 313000China Key Laboratory of Multiomics Research and Clinical Transformation of Digestive Cancer of Huzhou HuzhouZhejiang 313000China The Fifth Affiliated Clinical Medical College of Zhejiang Chinese Medical University HangzhouZhejiang 313000China Zhejiang Provincial People's Hospital and Affiliated People's Hospital Hangzhou Medical CollegeHangzhouZhejiang 310000China

With the rapid development of histological techniques and the widespread applica-tion of single-cell sequencing in eukaryotes,researchers desire to explore individual microbial.genotypes and functional expression,which deepens our understanding of microorganisms.In this review,the history of the development of microbial detection technologies was revealed and the difficulties in the application of single-cell sequencing in microorganisms were dissected as well.Moreover,the characteristics of the currently emerging microbial single-cell sequencing(Microbe-seq)technology were summarized,and the prospects of the application of Microbe-seq in microorganisms were distilled based on the current development status.Despite its mature development,the Microbe-seq technology was still in the optimization stage.A retrospective study was conducted,aiming to promote the widespread application of single-cell sequencing in microorganisms and facilitate further improvement in the technol-ogy.

关键词： Bacterialantibiotic resistance Host immunity Host-phage interaction Microbial single-cell sequencing Microorganisms Single cell Single-cell sequencing technology

Electric-controlled pressure relief valve for enhanced safety in liquid-cooled lithium-ion battery packs

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Journal of Energy Chemistry 2024年第3期90卷 98-109,I0004页

作者： Yuhang Song Jidong Hou Nawei Lyu Xinyuan Luo Jingxuan Ma shuwen Chen Peihao wu Xin Jiang Yang Jin Research Center of Grid Energy Storage and Battery Application School of Electrical and Information EngineeringZhengzhou UniversityZhengzhou 450001HenanChina

The liquid-cooled battery energy sto rage system(LCBESS) has gained significant attention due to its superior thermal management capacity.However,liquid-cooled battery pack(LCBP) usually has a high sealing level above IP65,which can trap flammable and explosive gases from battery thermal runaway and cause explosions.This poses serious safety risks and challenges for LCBESS.In this study,we tested overcharged battery inside a commercial LCBP and found that the conventionally mechanical pressure relief valve(PRV) on the LCBP had a delayed response and low-pressure relief efficiency.A realistic 20-foot model of an energy storage cabin was constructed using the Flacs finite element simulation software.Comparative studies were conducted to evaluate the pressure relief efficiency and the influence on neighboring battery packs in case of internal explosions,considering different sizes and installation positions of the PRV.Here,a newly developed electric-controlled PRV integrated with battery fault detection is introduced,capable of starting within 50 ms of the battery safety valve opening.Furthermore,the PRV was integrated with the battery management system and changed the battery charging and discharging strategy after the PRV was opened.Experimental tests confirmed the efficacy of this method in preventing explosions.This paper addresses the safety concerns associated with LCBPs and proposes an effective solution for explosion relief.

关键词： Pressure relief valve Liquid-cooled battery pack Explosion Flacs

Engineered extracellular vesicles efficiently deliver CRISPR-Cas9 ribonucleoprotein(RNP)to inhibit herpes simplex virus1 infection in vitro and in vivo

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Acta Pharmaceutica Sinica B 2024年第3期14卷 1362-1379页

作者： Yuanda Wan Liren Li Ruilin Chen Jiajia Han Qiyun Lei Zhipeng Chen Xiaodong Tang Wenyu wu shuwen Liu Xingang Yao NMPA Key Laboratory for Research and Evaluation of Drug Metabolism Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Center of Clinical Pharmacy Nanfang HospitalSouthern Medical UniversityGuangzhou 510515China State Key Laboratory of Ophthalmology Zhongshan Ophthalmic CenterSun Yat-sen UniversityGuangzhou 510060China Key Laboratory of Infectious Diseases Research in South China(Southern Medical University) Ministry of EducationGuangzhou 510515China

Extracellular vesicles(EVs)have recently emerged as a promising delivery platform for CRISPR/Cas9 ribonucleoproteins(RNPs),owing to their ability to minimize off-target effects and immune responses.However,enhancements are required to boost the efficiency and safety of Cas9 RNP enrichment within EVs.In response,we employed the Fc/Spa interaction system,in which the human Fc domain was fused to the intracellular domain of PTGFRN-Δ687 and anchored to the EV membrane.Simultaneously,the B domain of the Spa protein was fused to the C domain of cargos such as Cre or spCas9.Due to the robust interaction between Fc and Spa,this method enriched nearly twice the amount of cargo within the EVs.EVs loaded with spCas9 RNP targeting the HSV1 genome exhibited significant inhibition of viral replication in vitro and in vivo.Moreover,following neuron-targeting peptide RVG modification,the in vivo dosage in neural tissues substantially increased,contributing to the clearance of the HSV1 virus in neural tissues and exhibiting a lower off-target efficiency.These findings establish a robust platform for efficient EV-based SpCas9 delivery,offering potential therapeutic advantages for HSV1 infections and other neurological disorders.

关键词： Extracellular vesicles CRISPR/Cas9 Ribonucleoproteins PTGFRN Fc/Spa HSV1 Neuron-targeting Delivery

糖转运蛋白SlSWEET12c负向调控番茄对细菌性叶斑病的抗性

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园艺学报 2024年第2期51卷 321-334页

作者：邢亚男程杰刘欣吕书文吴媛媛姜晶沈阳农业大学园艺学院沈阳110866 辽宁省农业科学院蔬菜研究所沈阳110161

根据番茄表达数据库(http://ted.***.cornell.edu/cgi-bin/)及实时定量分析发现,糖转运蛋白(sugars will eventually be exported transporters)基因SlSWEET12c在番茄感病品种和抗病品种中表达差异最明显。以番茄(Solanum lycopersicum)栽培品种‘中蔬6号’为试材,获得了稳定遗传的SlSWEET12c沉默(RNAi)和过表达(OE)植株。进一步接种细菌性叶斑病病菌丁香假单胞菌(Pst DC3000)后,通过对叶片中细菌生长量、Fv/Fm变化,可溶性糖和淀粉含量、活性氧积累及防御酶活性和PR蛋白相关基因表达情况的分析,明确SWEET12c在番茄抵御PstDC3000过程中的作用。与野生型相比,RNAiSlSWEET12c植株接种Pst DC3000后叶片中的细菌生长量、病斑数明显减少,对Pst DC3000的抗性增强,而OE-SlSWEET12c的叶片发病更严重,细菌菌落数及褐色病斑数增多。RNAi-SlSWEET12c植株在接菌后蔗糖和淀粉含量明显高于野生型,活性氧积累少于野生型,防御酶(POD、SOD、APX)活性和PR蛋白相关基因(NPR1、PR1、PR2、PR5)表达明显升高,而OE-SlSWEET12c植株蔗糖和淀粉含量低于野生型,活性氧积累高于野生型,防御酶活性和PR蛋白相关基因表达明显低于野生型。以上结果表明SlSWEET12c通过影响叶片中糖分的组成,负向调控番茄对细菌性叶斑病的抗性。

关键词：番茄糖转运蛋白细菌性叶斑病 SlSWEET12c

分子动力学模拟在分散剂/表面活性剂在煤颗粒表面吸附机理方面的研究进展

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燃料化学学报（中英文） 2024年第3期52卷 452-460页

作者：吕冬梅吴慧君陈健朋聂云昊边国琴李静龚树文聊城大学化学化工学院山东聊城252000

本研究从力场、几何优化、牛顿运动方程、周期性边界条件、系宗、控温控压方法、步长步数等方面简述了分子动力学模拟的基本原理。目前,煤大分子的构建方法有三种:经典模型、自主构建模型和含氧官能团修饰的石墨烯层模型。分子动力学模... 详细信息

本研究从力场、几何优化、牛顿运动方程、周期性边界条件、系宗、控温控压方法、步长步数等方面简述了分子动力学模拟的基本原理。目前,煤大分子的构建方法有三种:经典模型、自主构建模型和含氧官能团修饰的石墨烯层模型。分子动力学模拟中,吸附构型的图像信息可以直接观察分散剂/表面活性剂在煤颗粒表面的吸附情况及吸附历程,而密度分布曲线、均方根位移、吸附能等定量结果可以揭示分散剂/表面活性剂的吸附机理。分子动力学模拟与实验方法相结合可以从微观和宏观两个角度揭示分散剂/表面活性剂在煤颗粒表面吸附模式,将为分散剂和浮选剂的开发和应用提供重要的理论支撑。

关键词：分子动力学模拟分散剂表面活性剂煤颗粒吸附机理

ANXA2 Facilitates Enterovirus 71 Infection by Interacting with 3D Polymerase and PI4KB to Assist the Assembly of Replication Organelles

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Virologica Sinica 2021年第6期36卷 1387-1399页

作者： Qiuhan Zhang Siliang Li Ping Lei Zixian Li Feifei Chen Qi Chen Yulu Wang Jiami Gong Qi Tang Xinjin Liu Ke Lan shuwen wu State Key Laboratory of Virology College of Life SciencesWuhan UniversityWuhan430072China Medical Research Institute Wuhan UniversityWuhan430072China Frontier Science Center for Immunology and Metabolism Wuhan UniversityWuhan430072China

Similar to that of other enteroviruses, the replication of enterovirus 71(EV71) occurs on rearranged membranous structures called replication organelles(ROs). Phosphatidylinositol 4-kinase Ⅲ(PI4KB), which is required by enteroviruses for RO formation, yields phosphatidylinositol-4-phosphate(PI4P) on ROs. PI4P then binds and induces conformational changes in the RNA-dependent RNA polymerase(Rd Rp) to modulate Rd Rp activity. Here, we targeted 3D polymerase, the core enzyme of EV71 ROs, and found that the host factor Annexin A2(ANXA2) can interact with 3 D polymerase and promote the replication of EV71. Then, an experiment showed that the annexin domain of ANXA2, which possesses membranebinding capacity, mediates the interaction of ANXA2 with EV71 3 D polymerase. Further research showed that ANXA2 is localized on ROs and interacts with PI4KB. Overexpression of ANXA2 stimulated the formation of PI4P, and the level of PI4P was decreased in ANXA2-knockout cells. Furthermore, ANXA2, PI4KB, and 3D were shown to be localized to the viral RNA replication site, where they form a higher-order protein complex, and the presence of ANXA2 promoted the PI4 KB-3D interaction. Altogether, our data provide new insight into the role of ANXA2 in facilitating formation of the EV71 RNA replication complex.

关键词： Enterovirus 71(EV71) Annexin A2(ANXA2) 3D polymerase Phosphatidylinositol 4-kinaseⅢ(PI4KB)

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毛坪铅锌矿采场热环境影响因素研究

矿业科学学报 2024年第2期9卷 270-277页

作者：康建宏田佳阔吴云韬崔数文李晴宋小林中国矿业大学安全工程学院江苏徐州221116 彝良驰宏矿业有限公司云南昭通657000

矿井热害是矿井深部开采面临的重要问题。以毛坪铅锌矿为工程案例,采用现场测试与数值模拟相结合的方法,研究了不同热源对深部矿井采场热环境的影响。基于现场实测的采场地温梯度与热环境参数,构建了毛坪铅锌矿采场物理模型,采用COMSOL... 详细信息

矿井热害是矿井深部开采面临的重要问题。以毛坪铅锌矿为工程案例,采用现场测试与数值模拟相结合的方法,研究了不同热源对深部矿井采场热环境的影响。基于现场实测的采场地温梯度与热环境参数,构建了毛坪铅锌矿采场物理模型,采用COMSOL软件分析了较低地温条件下风流温度、人体热源、机电设备以及充填体四种热源对采场及围岩温度的影响,并通过现场温度测量数据对数值计算结果进行验证。结果表明:毛坪铅锌矿目前开采水平地温为21.60℃,地温梯度为1.66℃/hm;灰砂比是影响充填体放热的重要因素,充填体产生的热量不仅会借助风流直接传至采场,还能通过围岩传导至巷道壁面,造成采场温度升高;地温对采场温度影响较大,采场温度随着地温的增大呈线性增加;毛坪铅锌矿中各个热源对采场环境的影响程度不同,其中风流能显著提高采场温度,充填体和机电设备次之,人体热源对采场温度的影响最小。

关键词：采场热环境低地温充填体风流温度数值模拟

导师制在血液内科规范化培训护士带教中的应用

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中国继续医学教育 2024年第6期16卷 129-133页

作者：罗凤莲吴霞邓淑文重庆医科大学附属第二医院血液科重庆400041 西南大学体育学院重庆400799

目的探索导师制在血液内科规范化培训护士带教工作中的应用效果。方法研究以2021年3月—2023年3月轮转到重庆医科大学附属第二医院血液内科的规范化培训护士70名为研究对象,对每轮规范化培训护士采用随机数表法分为对照组和观察组,其... 详细信息

目的探索导师制在血液内科规范化培训护士带教工作中的应用效果。方法研究以2021年3月—2023年3月轮转到重庆医科大学附属第二医院血液内科的规范化培训护士70名为研究对象,对每轮规范化培训护士采用随机数表法分为对照组和观察组,其中对照组34名,观察组36名。对照组接受传统的带教模式,由资深护士指导学习和实践;观察组实施导师制带教模式,由专业导师负责培养和辅导。通过对比2组护士的理论知识、操作技能、综合素质和教学满意度等指标,评价导师制教学模式在规范化培训护士培训中的应用效果。结果观察组的理论知识成绩为(93.00±2.28)分、操作技能成绩为(93.36±1.66)分、综合素质考核成绩为(97.11±1.47)分、教学满意度为100%,高于对照组[理论知识成绩(89.65±6.71)分、操作技能成绩(91.44±3.93)分、综合素质考核成绩(94.18±2.89)分、教学满意度83.60%],差异有统计学意义(P <0.05)。结论对血液科规范化培训护士实施导师制教学模式,可以使规范化培训护士的专科理论知识、操作技能、综合素质等方面的能力得到全面的提高。

关键词：导师制血液内科规范化培训护士临床带教