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Inexpensive Procedure for Measurement of Ethanol: Application to Bioethanol Production Process

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Advances in Microbiology 2017年第11期7卷 743-748页

作者： Roni Miah Ayesha Siddiqa Jamsheda Ferdous Tuli Noyon Kumar Barman shuvra Kanti dey Nihad Adnan Mamoru Yamada Ali Azam Talukder Department of Microbiology Jahangirnagar University Dhaka Bangladesh Department of Biological Chemistry Yamaguchi University Yamaguchi Japan

We have developed a spectrophotometric method for measurement of ethanol concentration in any unknown sample using a solvent tri-n-butyl phosphate [TBP, non-alcoholic solvent, density = 0.975 to 0.976, solubility in water = 0.028% (w/v)]. Solvent TBP separates ethanol from the non-hydrolyzed substrates that could interrupt the desired result by reacting with dichromate reagent. Oxidation of ethanol with dichromate reagent imparts blue green-colour to the solvent, which is easily detected by Spectrophotomer at 595 nm. Our established method showed similar results performed by relatively expensive Gas Chromatography (GC) method. In our present study we put forth a cheap alternate method for determining ethanol concentration in any aqueous solution.

关键词： Bioethanol Yeasts Saccharomyces cerevisiae

Optimization of Acetic Acid Production Rate by Thermotolerant <i>Acetobacter</i>spp.

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Advances in Microbiology 2017年第11期7卷 749-759页

作者： Tahsina Islam Farzana Diba Roni Miah Ayesha Siddiqa Nafisa Azmuda Shamsun Nahar Nihad Adnan shuvra Kanti dey Ali Azam Talukder Department of Microbiology Jahangirnagar University Dhaka Bangladesh Department of Microbiology University of Dhaka Dhaka Bangladesh

Thermotolerant microorganisms were collected, identified and characterized under different physiological conditions from various rotten fruits in Bangladesh for vinegar production. Among the 15-isolates characterized previously, the strains F-1, F-3 and F-10 represented Staphylococcus, Bacillus and Acetobacter spp., respectively. After checking various parameters for growth, acetic acid production rate was optimized further. Among the 3-starins analyzed here, the strain F-10 gave maximum acetic acid (7.0 g/100 ml) at 37°C in 2% ethanol concentration. The strain F-10 is capable of producing high yield of acetic acid at relatively high temperature, which is an ideal condition for vinegar production, which may reduce the water cooling expenses as well as the risk of contamination.

关键词： Decomposed Fruits Materials Thermotolerant Microorganisms Acetobacter Spp. Acetic Acid Vinegar Production

Genome Annotation and Comparative Genomics of ORF Virus

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Advances in Microbiology 2014年第15期4卷 1117-1131页

作者： A. K. M. Firoj Mahmud K. M. Zillur Rahman shuvra Kanti dey Tahsina Islam Ali Azam Talukder Molecular Biology Department Umea University Umea Sweden Deptartment of Microbiology Jahangirnagar University Savar Bangladesh

ORF virus (ORFV), the etiological agent of contagious pustular dermatitis in small ruminants, belongs to members of the genus Parapoxvirus of the Poxviridae. The genome of the ORFV is dsDNA of 139,962 bp which has about 89% coding region, 63% GC content and codes 130 proteins. There are four unique genes within the genome revealed by homology search of them two posses’ strong regulatory region and transmembrane helices. One of the ORF-039 contains signal peptide indicating the possibilities to be secretory protein coding gene. Comparative genomic analysis reveals significant differences in Bovine Papular Stomatitis Virus (BPSV) strain BV-AR02 and ORFV strain OV-SA00, and these may account for differences in host range. Interspecies sequence variability is observed in all functional classes of genes but is the highest in putative virulence/host range genes. Notably, ORFV contains genes which are homologous of Vaccinia virus. Phylogenetic analysis reveals that although divergent, ORFV virus is distinct from other known mammalian cowpox virus. An improved understanding of Parapoxvirus (PPV) biology will permit the engineering of novel vaccine viruses and expression vectors with enhanced efficacy and greater versatility. The novel vaccine will have a significant role in the economy of a country through the control of disease in an economically important and small ruminant caused by ORFV.

关键词： ORF Virus Contagious Pustular Dermatitis Comparative Genomics GLIMMER ACT Uniue Genes Novel Vaccine

Antimicrobial Activity of Jambul (<i>Syzygium cumini</i>) Fruit Extract on Enteric Pathogenic Bacteria

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Advances in Microbiology 2017年第3期7卷 195-204页

作者： Razoanul Haque Mariya Kibtiya Sumiya Najmuj Sakib Omar Sadi Sarkar Tanjir Tarek Ibn Siddique Saddam Hossain Anisul Islam Anowar Khasru Parvez Ali Azam Talukder shuvra Kanti dey Department of Microbiology Jahangirnagar University Dhaka Bangladesh

Jambul (Syzygium cumini) contain several biological activities including anti diabetic, anti-inflammatory, gastroprotective and antibacterial activity. In this study, we investigated antibacterial activity of Jambul juice extract against some common enteric pathogens like Salmonella typhimurium, Shigella flexneri, Staphylococcus aureus, and ETEC (Entero toxigenic E. coli). Growth inhibition of these entero pathogenic bacteria was measured by growing in Nutrient Broth media supplemented with 0%, 5%, 10% and 25% juice extract and then plating on Nutrient Agar plate for colony count at 0, 24 and 48 hours time points. The effect of Jambul juice on the growth of Lactobacillus acidophilus and Lactobacillus bulgaricus were also investigated. We observed that the growth of Salmonella typhimurium, Shigella flexneri, Staphylococcus aureus, and ETEC were significantly inhibited by Jambul juice by 1 to 6 logs (p Lactobacillus acidophilus and Lactobacillus bulgaricus) were not affected significantly. These findings indicate that Jambul juice have selective bactericidal effects against several enteric pathogens while beneficial species remain unaffected. To far our knowledge, this is the first report about the antibacterial activity of fruit juice in Bangladesh.

关键词： Jambul Foodborne Pathogens Fruit Extract Probiotics Antimicrobial Activity