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Effects of dietary tributyrin on intestinal mucosa development,mitochondrial function and AMPK-mTOR pathway in weaned pigs

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Journal of Animal Science and Biotechnology 2020年第2期11卷 602-610页

作者： Chunchun Wang shuting cao Zhuojun Shen Qihua Hong Jie Feng Yan Peng Caihong Hu Key Laboratory of Molecular Animal Nutrition Ministry of EducationAnimal Science CollegeZhejiang UniversityYu Hang Tang Rd No.866Hangzhou 310058People’s Republic of China Shanghai Menon Animal Nutrition Technology Co.Ltd. Shanghai 201807China

Background:The objective of this experiment was to investigate the influence of dietary tributyrin on intestinal mucosa development,oxidative stress,mitochondrial function and AMPK-mTOR signaling ***:Seventy-two pigs were divided into two treatments and received either a basal diet or the same diet supplemented with 750 mg/kg *** treatment has six replicates of six *** 14 days,6 pigs from each treatment were selected and the jejunal samples were ***:Results showed that supplemental tributyrin increased(P<0.05)villus height and villus height:crypt depth of weaned *** fed tributyrin had greater(P<0.05)RNA/DNA and protein/DNA ratios than pigs on the control *** mRNA levels of sodium glucose transport protein-1 and glucose transporter-2 in the jejunum were upregulated(P<0.05)in pigs fed the tributyrin *** tributyrin supplementation lowered(P<0.05)the malondialdehyde and hydrogen peroxide(H2O2)content in jejunum,enhanced(P<0.05)the mitochondrial function,as demonstrated by decreased(P<0.05)reactive oxygen species level and increased(P<0.05)mitochondrial membrane ***,tributyrin increased(P<0.05)mitochondrial DNA content and the mRNA abundance of genes related to mitochondrial functions,including peroxisomal proliferator-activated receptor-γcoactivator-1α,mitochondrial transcription factor A,nuclear respiratory factor-1 in the *** with tributyrin elevated(P<0.05)the phosphorylation level of AMPK and inhibited(P<0.05)the phosphorylation level of mTOR in jejunum compared with the control ***:These findings suggest that dietary supplementation with tributyrin promotes intestinal mucosa growth,extenuates oxidative stress,improves mitochondrial function and modulates the AMPK-mTOR signal pathway of weaned pigs.

关键词： AMPK-mTOR signaling pathway Intestinal mucosa development Mitochondrial function Tributyrin Weaned pigs

Alterations in intestinal microbiota composition coincide with impaired intestinal morphology and dysfunctional ileal immune response in growing-finishing pigs under constant chronic heat stress

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Journal of Animal Science and Biotechnology 2022年第3期13卷 769-786页

作者： Yunxia Xiong shuting cao Hao Xiao Qiwen Wu Hongbo Yi Zongyong Jiang Li Wang State Key Laboratory of Livestock and Poultry Breeding Key Laboratory of Animal Nutrition and Feed Science in South China Ministry of AgricultureMaoming BranchGuangdong Laboratory for Lingnan Modern AgricultureGuangdong Key Laboratory of Animal Breeding and NutritionInstitute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640China

Background:Previous studies had shown that short-term acute heat stress(HS)affected the host’s metabolism and intestinal microbiota independent of feed intake(FI)reduction,and long-term calorie restriction caused intestinal morphological injuries and gut microbial ***,research on the effects of constant chronic HS on intestinal microbial composition and the roles of FI reduction played in is *** study aimed to investigate the effects of 7-day constant chronic HS on the composition of intestinal microbes in growing-finishing pigs,and its relationship with pigs’performance,intestinal morphology,and ileal immune ***-four growingfinishing pigs(Duroc×Large White×Landrace,30±1 kg body weight)were randomly assigned to three treatments(n=8),1)thermal neutral(TN)conditions(25±1℃)with ad libitum FI,2)HS conditions(35±1℃)with ad libitum FI,3)pair-fed(PF)with HS under TN conditions to discriminate the confounding effects of dissimilar FI,and the FI was the previous day’s average FI of *** small intestinal segments(duodenum,jejunum,and ileum)and feces were collected on d ***:Results indicated that HS drastically declined(P<0.05)average daily gain(ADG)and average daily feed intake(ADFI)(about 61%)in comparison with TN,and caused hyperpyrexia,meanwhile PF caused *** observation by light and electron microscopes showed that both HS and PF treatment decreased(P<0.05)the villus and microvillus height compared with ***,HS increased(P<0.05)protein expression of heat shock protein 70 in the duodenum,jejunum,and ***,the expression of tight junction protein zonula occluden-1(ZO-1)in the duodenum and ileum,and Occludin in the ileum were enhanced(P<0.05)compared with TN and ***,HS significantly enhanced(P<0.05)the mRNA relative expression of inflammatory cytokines(TLR-2,TLR-4,and tumor necrosis factor-α(TNF-α),IL-6,IL-8,PG1–5,β-defensin 2(pBD-2)),mucins(mucin-1 and mucin-2)and P65 protein l

关键词： Constant chronic heat stress Growing-finishing pigs Ileal immune response Intestinal microbiota Performance

Preparation, characterization, antimicrobial and cytotoxicity studies of copper/zincloaded montmorillonite

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Journal of Animal Science and Biotechnology 2017年第3期8卷 670-676页

作者： Lefei Jiao Fanghui Lin shuting cao Chunchun Wang Huan Wu Miaoan Shu Caihong Hu Animal Science College Zhejiang UniversityKey Laboratory of Animal Feed and Nutrition of Zhejiang ProvinceHangzhou 310058People's Republic of China Key Laboratory of Animal Nutrition and Feed in East China Ministry of AgricultureHangzhou 310058People's Republic of China

Background： A series of modified montmorillonites（Mt） including zinc-loaded Mt（Zn-Mt）, copper-loaded Mt（Cu-Mt）, copper/zinc-loaded Mt with different Cu/Zn ratio（Cu/Zn-Mt-1, Cu/Zn-Mt-2, Cu/Zn-Mt-3） were prepared by an ion-exchange reaction, and characterized using X-ray diffraction（XRD）, fourier transformed infrared spectroscopy（FTIR） and transmission electron microscopy（TEM）. The specific surface areas, antimicrobial activity and cytotoxicity of the modified Mt were ***： In the modified Mt, hydrated Cu ions and Zn ions were exchanged in the interlayer space of Mt and the particles were irregular shapes. The results showed that Cu/Zn-Mt enhanced antibacterial and antifungal activity compared with Zn-Mt and Cu-Mt possibly due to the synergistic effect between Cu and Zn. Among the Cu/Zn-Mt with different Cu/Zn raitos, Cu/Zn-Mt with a Cu/Zn ratio of 0.98 or 0.51 showed higher antimicrobial activity against gram-negative bacteria（Escherichia coli）, gram-positive bacteria（Staphylococcus aureus）, fungi（Candida albicans）.Moreover, the antimicrobial activity of Cu/Zn-Mt was correlated with its specific surface area. Cytotoxicity studies on IPEC-J2 cell showed a slight cytotoxicity of Cu/***： The current data provide clear evidence that in terms of its antimicrobial activity and relatively low toxicity, the Cu/Zn-Mt holds great promise for applications in animal husbandry.

关键词： Antimicrobial reagent Modified montmorillonites Synergistic antimicrobial effect

Accurate models and nutritional strategies for specific oxidative stress factors: Does the dose matter in swine production?

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Journal of Animal Science and Biotechnology 2024年第2期15卷 519-534页

作者： Changming Hong Yujian Huang shuting cao Li Wang Xuefen Yang Shenglan Hu Kaiguo Gao Zongyong Jiang Hao Xiao State Key Laboratory of Swine and Poultry Breeding Industry Ministry of Agriculture Key Laboratory of Animal Nutrition and Feed Science in South ChinaGuangdong Public Laboratory of Animal Breeding and NutritionGuangdong Provincial Key Laboratory of Animal Breeding and NutritionMaoming BranchGuangdong Laboratory for Lingnan Modern AgricultureGuangzhouChina Institute of Animal Science Guangdong Academy of Agricultural Sciences1 Dafeng 1st StreetGuangzhou 510640China

Oxidative stress has been associated with a number of physiological problems in swine,including reduced production ***,although there has been increased research into regulatory mechanisms and antioxidant strategies in relation to oxidative stress-induced pig production,it remains so far largely unsuccessful to develop accurate models and nutritional strategies for specific oxidative stress ***,we discuss the dose and dose intensity of the causes of oxidative stress involving physiological,environmental and dietary factors,recent research models and the antioxidant strategies to provide theoretical guidance for future oxidative stress research in swine.

关键词： Accurate models Dose Nutritional strategies Oxidative stress Swine

GH4061合金在高压富氧环境下的燃烧行为

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金属学报 2023年第4期59卷 547-555页

作者：曹姝婷张少华张健中国科学院金属研究所沈阳110016 中国科学技术大学材料科学与工程学院沈阳110016

基于自主研制的金属富氧燃烧实验设备,在O_(2)浓度为99.5%、压强为3.5~25 MPa条件下,对GH4061合金进行了燃烧实验,使用高速相机、SEM、EDS和XRD对合金燃烧过程以及燃烧后的试棒进行观察分析,研究了高压富氧条件下GH4061合金的燃烧过程,... 详细信息

基于自主研制的金属富氧燃烧实验设备,在O_(2)浓度为99.5%、压强为3.5~25 MPa条件下,对GH4061合金进行了燃烧实验,使用高速相机、SEM、EDS和XRD对合金燃烧过程以及燃烧后的试棒进行观察分析,研究了高压富氧条件下GH4061合金的燃烧过程,并分析了其燃烧机理。结果表明,随着O_(2)压强的提高,试棒燃烧长度增大,同时燃烧速率加快。根据ASTM-G124标准,GH4061合金在99.5%纯O_(2)室温点燃条件下,燃烧阈值约为5 MPa。合金的燃烧区域自上而下可以分为过渡区、熔化区、燃烧前沿和氧化物区。燃烧过程中,燃烧热较高的元素优先燃烧,液态金属与O_(2)接触发生反应形成熔融的氧化物,其中密度较低的氧化物会上浮至熔化区,并在上浮过程中凝固形成尺寸较小的富Al/Ti氧化物和尺寸较大、具有枝晶形貌的混合氧化物。基于热力学理论,分析了O_(2)压强对合金燃烧行为的影响。

关键词：金属燃烧高压富氧高温合金燃烧机理

益生菌对断奶仔猪肠道屏障功能影响的Meta分析

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动物营养学报 2022年第5期34卷 3317-3328页

作者：高靖春曹舒婷蒋宗勇王丽广东省农业科学院动物科学研究所农业部华南动物营养与饲料重点实验室畜禽育种国家重点实验室岭南现代农业科学与技术广东省实验室茂名分中心广东省畜禽育种与营养重点实验室广州510640 仲恺农业工程学院广州510225

为探究益生菌添加对断奶仔猪肠道屏障功能带来的影响,本次试验对相关方面的研究进行了Meta分析。通过计算机检索中国知网、维普、Science Direct、Web of Science数据库,检索益生菌添加对断奶仔猪肠道健康影响的随机对照试验。检索年限... 详细信息

为探究益生菌添加对断奶仔猪肠道屏障功能带来的影响,本次试验对相关方面的研究进行了Meta分析。通过计算机检索中国知网、维普、Science Direct、Web of Science数据库,检索益生菌添加对断奶仔猪肠道健康影响的随机对照试验。检索年限从建库到2021年4月,设定样本纳入及排除标准进行文献筛选、数据提取,纳入总样本720例,用R语言对纳入研究进行相关分析,效应指标选择标准化均数差(SMD)。Meta分析结果显示:饲粮添加益生菌可以有效缓解断奶仔猪腹泻[P<0.01,SMD=-15.15,95%置信区间(95%CI)为-21.55~8.76],提高闭锁小带蛋白-1(ZO-1)(P<0.01,SMD=20.83,95%CI为8.84~32.83)、闭合蛋白(Occludin)(P <0.01,SMD=2.587,95%CI为0.67~4.50)的表达量,提高空肠绒毛高度(P<0.01,SMD=15.22,95%CI为5.91~24.54)。以上结果提示,益生菌对断奶仔猪肠道屏障功能具备保护作用。

关键词：益生菌断奶仔猪肠道屏障功能 Meta分析

Cu含量对K4061合金显微组织和拉伸性能的影响

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金属学报 2024年第9期60卷 1179-1188页

作者：曹姝婷赵剑巩桐兆张少华张健中国科学院金属研究所师昌绪先进材料创新中心沈阳110016 中国科学技术大学材料科学与工程学院沈阳110016 西安航天动力研究所西安710100

随着我国液氧煤油火箭发动机推力的不断提高,亟需一种强度更高、抗富氧燃烧性能更好的新型高温合金材料。K4061合金属于第二代抗富氧烧蚀高温合金,与第一代抗富氧烧蚀高温合金相比,合金成分的显著特点是添加了Cu元素。但是,目前关于Cu... 详细信息

随着我国液氧煤油火箭发动机推力的不断提高,亟需一种强度更高、抗富氧燃烧性能更好的新型高温合金材料。K4061合金属于第二代抗富氧烧蚀高温合金,与第一代抗富氧烧蚀高温合金相比,合金成分的显著特点是添加了Cu元素。但是,目前关于Cu在高温合金中的作用机制研究较少。因此,本工作在热力学相平衡计算的基础上,结合DSC、SEM以及TEM实验,研究了添加(1%~10%)Cu(质量分数)对K4061合金析出相以及Cu元素分布的影响。热力学计算结果表明,K4061合金在平衡凝固过程中,首先从液相中析出γ基体,随着液相溶质元素成分逐步提高,在凝固末期又从液相中析出MC型碳化物。此外,Cu的添加对于K4061合金的平衡凝固路径没有显著影响,但会降低K4061合金的固/液相线温度和MC的析出温度。实验结果表明,与平衡凝固不同,在K4061合金的实际凝固过程中,除了γ基体和MC型碳化物之外,在凝固末期还会析出δ相。不同Cu含量的K4061合金中析出相种类并未改变,这与热力学计算结果一致,但是在添加Cu的K4061合金中未观察到富Cu相并且Cu元素也没有偏聚到晶界或者碳化物相中。TEM观察结果表明,Cu富集在强化相内,且会增大强化相尺寸。此外,Cu的添加会降低合金的室温和750℃高温抗拉强度。

关键词：镍基高温合金凝固析出相 Cu 拉伸性能

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梯度流体剪应力下细胞膜张力的数值模拟

医用生物力学 2024年第4期39卷 600-606页

作者：曹淑婷赵森张笑高颜霍波北京理工大学宇航学院北京100081 军事科学院系统工程研究院北京100091 首都体育学院体育人工智能研究院北京100191

目的分析梯度流体剪应力(fluid shear stress,FSS)流场中细胞膜张力分布。方法构建梯度平板流动腔模型,采用流固耦合数值模拟方法,研究不同FSS梯度和幅值、不同静水压下细胞膜的膜张力分布。结果当流动腔入口流量增大时,FSS流场梯度呈... 详细信息

目的分析梯度流体剪应力(fluid shear stress,FSS)流场中细胞膜张力分布。方法构建梯度平板流动腔模型,采用流固耦合数值模拟方法,研究不同FSS梯度和幅值、不同静水压下细胞膜的膜张力分布。结果当流动腔入口流量增大时,FSS流场梯度呈正比例增大。梯度FSS流场下,细胞膜张力由贴壁侧向顶部呈先减小后增大的趋势。在正常人体血压下,静水压越大,细胞膜张力越大。当FSS幅值一定时,增加FSS梯度,细胞高、低FSS区域平均膜张力差值增大;当FSS梯度一定时,增加FSS幅值,细胞高、低FSS区域平均膜张力差值增大。结论梯度FSS流场会引起细胞膜张力的局部差异,其可能是破骨前体细胞在梯度流场中定向迁移的重要原因。

关键词：梯度平板流动腔梯度流体剪应力破骨前体细胞膜张力

维普期刊数据库博看期刊

Spherical nucleic acids for biomedical applications

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Advanced Sensor and Energy Materials

Advanced Sensor and Energy Materials 2024年第4期3卷 37-49页

作者： Chunxin Xia Hemei Cheng Xinwei Hou Yu Zhang Xinchi Zhou Qinglong Yan shuting cao Jiaxing Key Laboratory of Bio-semiconductor Xiangfu LaboratoryJiashan 314102China Nano-translational Medicine Research Center Yangtze Delta Region Institute of Tsinghua UniversityJiaxing 314000China The Interdisciplinary Research Center Shanghai Synchrotron Radiation FacilityShanghai Advanced Research InstituteChinese Academy of SciencesShanghai 201210China

Spherical nucleic acids(SNAs)are a 3D spherical nanostructure composed of highly oriented,dense layers of oligonucleotides conjugated to a hollow or solid *** structure allows SNAs to show resistance to nuclease degradation,enter into nearly all cells without transfection agents and enable precise interactions with target *** on superior biological properties,SNAs can be tailored for diverse biological applications,rendering them a flexible and biosafe tool for biological applications as well as an enabling platform for *** this review,we mainly discuss the structure and conjugation mode of SNAs and focus on recent advances in their applications,such as biomedical detection,imaging,and drug ***,the remaining challenges and future directions of SNAs are also discussed and proposed.

关键词： Spherical nucleic acids Conjugation mode Biomedical detection Imaging Drug delivery