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Localization of muscarinic acetylcholine receptor in plant guard cells

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Chinese Science Bulletin 2001年第7期46卷 586-588,618页

作者： Fanxia Meng Xin Liu shuqiu zhang Chenghou Lou China Agr Univ Coll Biol Sci Minist Agr Key Lab Plant Physiol & Biochem Beijing 100094 Peoples R China

Acetylcholine (ACh), as an important neuro-transmitter in animals, also plays a significant role in various kinds of physiological functions in plants. But relatively little is known about its receptors in plants. A green fluorescence BODIPY FL-labeled ABT, which is a high affinity ligand of muscarinic acetylcholine receptor (mAChR), was used to localize mAChR in plant guard cells. In Vicia faba L. and Pisum sativum L., mAChR was found both on the plasma membrane of guard cells. mAChR may also be distributed on guard cell chloroplast membrane of Vicia faba L. The evidence that mAChR localizes in the guard cells provides a new possible signal transduction pathway in ACh mediated stomata movement.

关键词： guard cell mAChR fluorescent localization Vicia faba L. Pisum sativum L.

Influence of Ganoderma lucidum spores on the levels of neuropeptide Y and somatostatin in brains of seizure rats

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Neural Regeneration Research 2008年第5期3卷 486-489页

作者： Kongli Zhu Ming Lu shuqiu Wang Shiling zhang Dixiang Sun Department of Pathophysiology School of Basic Medical Sciences Jiamusi University Jiamusi 154007 Heilongjiang Province China

BACKGROUND： Previous research has revealed that somatostatin can induce epilepsy, and that the levels of neuropeptide Y may increase and become more active in brain areas with epileptic seizures. OBJECTIVE： To observe the effect of Ganoderma luciclum spore powder on the neuropeptide Y and somatostatin content in the cerebral cortex and hippocampal regions of seizure rats induced by pentylenetetrazol （PTZ）. Furthermore, to verify any effect of Ganoderma lucidum spore powder on inhibition of epileptic seizures. DESIGN, TIME AND SETTING： A randomized group animal study was performed in August 2007 in the School of Basic Medical Sciences, Jiamusi University （Jiamusi, Heilongjiang, China）. MATERIALS： Thirty healthy, male, Wistar rats, aged 12 weeks and weighing 180-220 g, were taken as the experimental animals. PTZ （Sigma Company, United States） was used to induce epilepsy. Ganoderma lucidum spores （Leyss, ex Fr variety） were purchased from Jiamusi City Wild Growing Case of the Ganoderma Lucidum （China）. Rabbit anti-somatostatin antibodies and secondary antibodies were purchased from Wuhan Boster Company （China）. Neuropeptide Y radioimmunoassay kit was purchased from Beijing Furui Biotechnology Company （China）. METHODS： Thirty rats were randomly divided into three groups： a control group, an epilepsy model group and a Ganoderma lucidum spore-treated group. Each group contained 10 animals. Rats in the epilepsy model group were treated with intraperitoneal injections of PTZ and gastric perfusion of physiologic saline. In the Ganoderma lucidum spore-treated group, intraperitoneal injection of PTZ and gastric perfusion of Ganoderma lucidum spore powder were administered. The blank control group was only administered with the physiological saline by intraperitoneal injection and gastric perfusion. MAIN OUTCOME MEASURES： Immunohistochemical staining and radioimmunoassay methods were used to observe the changes of somatostatin and neuropeptide Y content in brain tissue of epile

关键词： Ganoderma lucidum spores epilepsy hippocampus somatostatin neuropeptide Y

Localization of NOS-like protein in guard cells of Vicia faba *** its possible function

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Chinese Science Bulletin 2007年第1期52卷 84-90页

作者： LIU Xin WANG YouQun JIA WenSuo LOU ChengHou zhang shuqiu State Key Laboratory of Plant Physiology and Biochemistry College of Biological Sciences China Agricultural University Beiiing 100094 China Department of Life Science Lai Yang Agricultural College Qingdao 266109 China

Using the immuno-fluorescence and immuno-gold electron microscope technology, localization of ni- tric oxide synthase (NOS)-like proteins was determined in guard cells of Vicia faba L. NOS is mainly localized in nucleus, cytoplasm, chloroplast, mitochondria and the cell wall of guard cells. Scorch and exogenous JA can enhance the level of nitric oxide (NO) and increase NOS activity in both leaf and epidermis, and the changing pattern of NOS activity was consistent with the change of NO. NOS in- hibitor, L-NAME, inhibited JA-induced NO generation. From the results, we presumed that NO genera- tion from NOS pathway is the main pathway in the stress and JA responses. The pharmacological ex- periment showed that increasing the Ca2+ at a suitable concentration promoted leaf NOS activity and the NO level, indicating that NOS activity together with the distribution of NO is Ca2+-dependent. NOS and NO are possibly involved in the regulation of stomatal movement thus playing an important role in plant stress responses.

关键词：免疫细胞氧化一氮连位化合物生物化学

Changes of biochemical indices in brain, liver tissue and serum in mice with Alzheimer disease after Chinese medicine treatment

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Neural Regeneration Research 2007年第5期2卷 291-296页

作者： Xiangyang Wang Lili zhang Haode Huang Qiang Qin Guimei Luo Chaogan Li shuqiu zhang Haierfu Research Center of Youjiang Medical College for Nationalities Baise 533000 Guangxi Zhuang Autonomous Region China

BACKGROUND： Alzheimer disease is a main type of dementia, and the important clinical characteristic is the rapid declines of memory and cognitive ability. OBJECTIVE： To study changes of biochemical indices in brain, liver tissue and serum, as well as memory of mice with Alzheimer disease after Chinese medicine treatment. DESIGN： A comparative animal experimental observation. SETTING： Haierfu Research Center of Youjiang Medical College for Nationalities. MATERIALS： Forty-eight healthy Kunming mice （24 males and 24 females）, 3 months old, were provided by the animal room of Youjiang Medical College for Nationalities. The animals were divided into four groups according to sex and body mass： control group, model group, Wuyuan Buxue treated group, Haierfu treated group, and 12 mice in each group. Wuyuan Buxue oral liquid was extracted from Polygonum multiflorum Thunb （red, radix） and longan meat （country medicine quasi- word B20020828）. Haierfu oral liquid was extracted from Yinhua, poriacocos, licorice, etc （Q/452600RYYLC01-92）. METHODS： The experiment was completed in Haierfu Research Center and Institute of Heavy Metal and Fluorosis-Arsenism of Youjiang Medical College for Nationalities from May 2006 to December 2006. ① All animals except those in the control group were given feed which was mixed with AlCl3 （12 g/L）, and they could freely drink 3 g/L Al（NO3）3. The mice in the control group were given normal feed. Wuyuan Buxue oral liquid and Haierfu oral liquid were distilled by distilled water for one time respectively. Five months after model establishment, mice in the Wuyuan Buxue treated group and Haierfu treated group were given intrapastric perfusion of Wuyuan Buxue oral liquid and Haierfu oral liquid respectively, and those in the model group and control group were given intrapastric perfusion of distilled water of the same volume. All the mice were treated for 45 days. ② The swimming time （s） and error times were determined with Y-shape water maze before and a

关键词： Alzheimer disease acetylcholinesterase mice

Reversing artemisinin resistance by leveraging thermo-responsive nanoplatform to downregulating GSH

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Chinese Chemical Letters 2024年第6期35卷 392-397页

作者： Yong-Dan Zhao Yidan Wang Rongrong Wang Lina Chen Hengtong Zuo Xi Wang Jihong Qiang Geng Wang Qingxia Li Canqi Ping shuqiu zhang Hao Wang School of Pharmacy Shanxi Medical UniversityTaiyuan 030001China National Center for Nanoscience and Technology Beijing 100190China Artemisinin Research Center Institute of Chinese Materia MedicaBeijing 100700China

Artemisinin(ART)resistance has been an emerging clinical problem,severely compromising antimalarial efficacy and threatening the global malaria elimination *** intensive studies about the molecular mechanism for ART resistance are under way,no effective therapeutic targets for reversing resistance have been ***,we explore glutathione(GSH)as a therapeutic target to develop a thermo-responsive nanoplatform to specifically co-deliver ART and GSH synthesis inhibitor(L-buthionine sulfoximine,BSO)in a sustained manner,effectively reversing ART resistance in *** combining with BSO,ART exerts increased antimalarial activity with reduced half-maximal inhibitory concentration(IC50)by 7.43-fold in ART-resistant *** work reveals that the GSH in ART-resistant parasites can be a promising therapeutic target for reversing ART resistance,paving the way for developing drug candidates and intelligent nanomedicines in malaria therapy.

关键词： Nanomedicines Drug delivery Artemisinins resistance Malaria Glutathione

A passive synthetic aperture phase correction algorithm for the asymmetric twin-line array sonar

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Chinese Journal of Acoustics 2007年第4期26卷 337-344页

作者： LI Yu zhang Yangfan HUANG Haining LI shuqiu LI Qihu zhang Chunhua Institute of Acoustics The Chinese Academy of Sciences Beijing 100080

A passive synthetic aperture based on phase correction algorithm for solving the port-starboard discrimination problem in the non-aligned towed twin-line array sonar, is described. This method creates a virtual array through applying the estimated phase correction into one array of twin-line arrays. Because the synthetic virtual array is aligned with the other array in twin-line arrays, the right port-starboard discriminated results can be obtained by array processing based on the new synthetic twin-line array. The effect of proposed method has been shown by simulated and sea-trials results in towed twin-line array sonar. With low extra computational loads, the proposed method is easy to apply to the practice.

关键词： correction algorithm synthetic aperture

水分胁迫诱导的离体蚕豆叶片与保卫细胞脱落酸积累

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中国农业大学学报 1999年第3期4卷 39-42页

作者：张蜀秋中国农业大学生物学院

研究了蚕豆离休叶片失水10％后，叶片与其气孔保卫细胞脱落酸含量的变化及其与叶龄的关系。未受胁迫叶片脱落酸含量在成熟叶片中高于幼嫩叶片，保卫细胞中脱落酸含量均在很低的水平。在叶片自然失水10％的10min内，叶片的脱落酸含量几... 详细信息

研究了蚕豆离休叶片失水10％后，叶片与其气孔保卫细胞脱落酸含量的变化及其与叶龄的关系。未受胁迫叶片脱落酸含量在成熟叶片中高于幼嫩叶片，保卫细胞中脱落酸含量均在很低的水平。在叶片自然失水10％的10min内，叶片的脱落酸含量几乎无变化，但保卫细胞的脱落酸含量增加5倍以上。4h后，叶片脱落酸含量也显著增加，从最幼嫩叶片到成熟叶片分别达到处理前的25，26，17，10和6倍。因是离休叶片，排除了脱落酸的其他来源，说明叶片在失去膨压时合成并积累脱落酸；所有保卫细胞脱落酸含量都增加30倍以上，以第2，3片完全展开叶片的保卫细胞最为敏感，其脱落酸含量增加40倍以上。失水处理后保持湿润恢复膨压的叶片和其保卫细胞的脱落酸含量没有增加。分析了在水分胁迫下保卫细胞中增加的脱落酸的可能来源。

关键词：水分胁迫诱导离体蚕豆叶片保卫细胞脱落酸积累

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四川地区含水气藏开采技术探讨

石油钻探技术 1999年第3期27卷 39-41页

作者：张数球中国新星石油公司开发部

在分析了四川地区含水气藏开发现状的基础上，总结了开采方式选择、气井出水后的技术措施及气田综合治理的一些经验。该地区气井不同的开采效果表明，含水气藏的开采，不但要严格按照合理的产量配产，而且要合理控制生产压差。

关键词：地层水气藏含水气藏气田天然气开采

Research on beamforming method for dual-line array with space mismatch between the two line arrays

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Chinese Journal of Acoustics 2008年第1期27卷 23-33页

作者： WANG Huakui LI Qihu HUANG Haining YE Qinghua LI shuqiu zhang Chunhua Institute of Acoustics Chinese Academy of Sciences Beijing 100080

The performance of port/starboard discrimination of dual-line array will be seriously degraded when the space between the two line arrays of the dual-line array is mismatched. The directivity function of the delay-sum and delay-subtract beamforming is deduced and the port/starboard discrimination of the delay-sum and delay-subtract beamforming is also studied both under the ideal status and the status under which the space between the two line arrays of the dual-line array is mismatched. Analysis results show the delay-subtract beamforming has better performance of port/starboard discrimination. At last, the sea trial results demonstrate the theoretical results.

关键词： line Research on beamforming method for dual-line array with space mismatch between the two line arrays