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辣椒小G蛋白CaROP的生物信息学分析

新疆农业科学 2024年第1期61卷 165-175页

作者：马思洁朱天生何璐杨叔青塔里木大学农学院新疆阿拉尔843300 南疆农业有害生物综合治理兵团重点实验室新疆阿拉尔843300

【目的】研究辣椒内小G蛋白CaROP的生物学功能、蛋白理化性质、蛋白结构及系统发育关系。【方法】运用ProtParam、ProtScale、SignalP5.0、TMHMM、NetPhos3.1和NetCGlyc1.0等生物信息学软件分析9个CaROP蛋白的蛋白理化特性;运用SOPMA和S... 详细信息

【目的】研究辣椒内小G蛋白CaROP的生物学功能、蛋白理化性质、蛋白结构及系统发育关系。【方法】运用ProtParam、ProtScale、SignalP5.0、TMHMM、NetPhos3.1和NetCGlyc1.0等生物信息学软件分析9个CaROP蛋白的蛋白理化特性;运用SOPMA和SWISS-MODEL等生物信息学软件预测分析9个CaROP蛋白的结构;运用生物信息学软件MEGA11分析9个CaROP蛋白的系统发育关系。【结果】9个CaROP蛋白的亲水性总平均值均小于0,均为亲水蛋白,其中6个为稳定的亲水蛋白;9个CaROP蛋白均无信号肽,即均为非分泌性蛋白;9个CaROP蛋白无跨膜结构域,即均非膜蛋白;9个CaROP蛋白均存在磷酸化位点,均无糖基化位点。9个CaROP蛋白的主要二级结构原件为无规则卷曲和α-螺旋,其次是β-折叠,最后是β-转角。9个CaROP蛋白聚为4个分支,其中分支Ⅰ和分支Ⅲ各包括1个CaROP蛋白。分支Ⅱ包括2个CaROP蛋白,其余5个CaROP蛋白均聚于分支Ⅳ中。【结论】9个CaROP蛋白具有完整的Rho功能域,均为非分泌性亲水蛋白。9个CaROP蛋白在系统进化树中共聚为了4个分支,其三级结构建模的预测结果也均较为理想,9个CaROP蛋白结构较稳定。

关键词：辣椒小G蛋白ROP 蛋白理化特性蛋白结构系统发育关系

土层厚度与灌溉方式对寒地水稻节水生长及产量的影响

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灌溉排水学报 2024年第3期43卷 27-32页

作者：卢程悦曹志富张万锋辛海霞杨树青内蒙古农业大学呼和浩特010018 乌兰浩特市农科局内蒙古乌兰浩特137400 内蒙古师范大学呼和浩特010018 科右前旗农牧业科学技术发展中心内蒙古乌兰浩特137708

【目的】探究不同土层厚度与灌溉方式对寒地水稻生长的影响。【方法】于2020年在兴安盟乌兰浩特市科右前旗袁隆平水稻试验基地开展水稻田间试验,试验设置15~20 cm(H1)、20~25 cm(H2)和25~30 cm(H3)3种土层厚度,设置控制灌溉(K)、常规灌... 详细信息

【目的】探究不同土层厚度与灌溉方式对寒地水稻生长的影响。【方法】于2020年在兴安盟乌兰浩特市科右前旗袁隆平水稻试验基地开展水稻田间试验,试验设置15~20 cm(H1)、20~25 cm(H2)和25~30 cm(H3)3种土层厚度,设置控制灌溉(K)、常规灌溉(CK)2种灌溉方式,共计6个处理,探究不同土层厚度与灌溉方式对寒地水稻生长的影响。【结果】K处理下的水稻株高相比CK矮,根长、茎粗、每穴有效穗数、千粒质量及产量相比CK均有不同程度的增加,H2和H3土层厚度下的水稻产量相比H1增加30.49%~32.91%和63.74%~76.33%,K处理下的总灌水量相比CK减少55.04%~56.84%,K处理下的灌溉水生产率相比CK增加1.55~1.86倍,H2和H3土层厚度下的水稻灌溉水生产率相比H1增加35.61%~38.62%和73.07%~94.14%。【结论】25~30 cm土层厚度下采用控制灌溉的水稻节水增产效果最优,适宜当地水稻种植,研究结果可为寒地水稻种植提供理论依据与技术参考。

关键词：土层厚度灌溉方式寒地水稻控制灌溉产量

基于FLAC^(3D)-COMSOL序贯耦合的钻割煤体三维卸压瓦斯流场数值分析

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西安科技大学学报 2024年第3期44卷 478-489页

作者：张浩郭军伟王穗芳范楠杨淑晴常家美雷倩茹晋能控股煤业集团有限责任公司山西大同037037 太原理工大学安全与应急管理工程学院山西太原030024

水力割缝技术通过高压水射流在煤层中构建人工裂隙,可实现低渗煤层的区域性卸荷增透和瓦斯高效抽采。然而,经典的COMSOL软件难以对钻割煤体的三维卸压瓦斯流场进行解算,严重制约了水力割缝技术的工程应用。鉴于此,采用FLAC3D-COMSOL序... 详细信息

水力割缝技术通过高压水射流在煤层中构建人工裂隙,可实现低渗煤层的区域性卸荷增透和瓦斯高效抽采。然而,经典的COMSOL软件难以对钻割煤体的三维卸压瓦斯流场进行解算,严重制约了水力割缝技术的工程应用。鉴于此,采用FLAC3D-COMSOL序贯耦合的数值解算方法,以中厚煤层钻割卸荷增透为例,对不同割缝半径、割缝厚度、埋深和割缝方式下钻割煤体的三维卸压瓦斯流场进行了数值分析。结果表明:水力割缝过程中,钻割煤体发生了卸荷损伤,形成了较大范围的圆台形塑性损伤区,煤体渗透率可大幅提高2~3个数量级;随着割缝半径和割缝厚度的增加,煤体的增透范围和瓦斯抽采半径逐渐增大,但在相同出煤量情况下增加割缝半径更有利于中厚煤层的卸荷增透和瓦斯抽采;深部高地应力环境导致煤层初始渗透率下降,但也可提高钻割煤体的增透范围和增透幅度,因此钻孔早期瓦斯抽采效果好,后期抽采效果差,在抽采过程中应尽量增加钻孔密度,缩短抽采周期;割缝方式对中厚煤层的整体卸压瓦斯抽采效果具有显著影响,单缝钻孔平行布置情况下煤层卸压瓦斯抽采效果最好,单缝交错布置情况下次之,而双缝间隔布置情况下最差。

关键词：低透煤层水力割缝卸荷损伤瓦斯抽采

苹果小G蛋白MdoROP的进化分类、结构及理化特性分析

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生物技术 2024年第1期34卷 35-46页

作者：刘礼瑶何璐赵博熊仁次杨叔青塔里木大学农学院新疆阿拉尔843300 塔里木大学园艺与林学学院新疆阿拉尔843300 南疆农业有害生物综合治理兵团重点实验室新疆阿拉尔843300

[目的]获取6个MdoROP蛋白并对其基序组成、系统进化关系、蛋白结构及理化特性进行分析。[方法]取苹果叶片进行离体接菌,利用SMARTS和MEGA 11等多种生物信息学软件对其进行预测分析。[结果]基于蛋白基序的组成差异可将MdoROP蛋白归为同... 详细信息

[目的]获取6个MdoROP蛋白并对其基序组成、系统进化关系、蛋白结构及理化特性进行分析。[方法]取苹果叶片进行离体接菌,利用SMARTS和MEGA 11等多种生物信息学软件对其进行预测分析。[结果]基于蛋白基序的组成差异可将MdoROP蛋白归为同一类且均无特异性基座,表明以上6个motif为6个MdoROP蛋白的保守基序。MdoROP蛋白长度介于197~211 aa之间;脂肪指数介于87.24~92.56之间;蛋白分子量介于21463.75~23587.2 Da;6个MdoROP蛋白的亲水性介于-0.125~-0.077且均为负值,6个MdoROP蛋白均为碱性亲水蛋白,均定位于细胞膜、细胞质和细胞核且均不具有信号肽和跨膜结构域;MdoROP蛋白的二级结构元件以无规则卷曲和α螺旋为主。[结论]6个MdoROP蛋白属于苹果小G蛋白ROP家族,是磷酸化蛋白和碱性亲水蛋白,均不是分泌蛋白和膜蛋白;无规则卷曲和α螺旋是6个MdoROP蛋白的主要二级结构,ROP基因三级结构的预测结果与二级构造相吻合。该文研究结果将为后续深入研究MdoROP蛋白的生物学功能奠定强有力的基础并提供理论支撑。

关键词：苹果小G蛋白MdoROP 系统进化关系理化特性蛋白结构

15NiMoSiCr10中速船用针阀体的热处理工艺开发

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热处理技术与装备 2024年第2期45卷 1-6页

作者：杨静翟寒萍冉启燕钟航阳树青李栋重庆红江机械有限责任公司重庆402162 船舶与海洋工程动力系统国家工程试验室重庆402162

针阀偶件是船用柴油机的一种重要零件,在船舶航行的安全性和可靠性中发挥关键作用。利用低压脉冲真空渗碳高压气淬技术,在国内率先成功开发了15NiMoSiCr10船用针阀体的热处理工艺,同时较系统地研究了其热处理性能特点。渗碳温度选用960... 详细信息

针阀偶件是船用柴油机的一种重要零件,在船舶航行的安全性和可靠性中发挥关键作用。利用低压脉冲真空渗碳高压气淬技术,在国内率先成功开发了15NiMoSiCr10船用针阀体的热处理工艺,同时较系统地研究了其热处理性能特点。渗碳温度选用960℃,采用VC-Sim 1.4.1专家软件对渗碳时间和乙炔脉冲进行仿真,降低工艺开发成本。960℃渗碳后直接进行18 bar高压氮气淬火,经-120℃冷处理2 h后分别在不同温度下进行抗回火性试验。试验结果表明,15NiMoSiCr10针阀体具有回火抗力好、金相组织优良以及芯部硬度较高等较好的工艺性,使用寿命超过6000 h,相比其他材料针阀体,其使用寿命得到大幅提高,有效提升了产品的市场竞争力,带来了较大的经济效益。

关键词：低压脉冲真空渗碳高压气淬热处理工艺 15NiMoSiCr10 针阀体

Recent advances in two-dimensional nanomaterials for bone tissue engineering

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Journal of Materiomics 2023年第5期9卷 930-958页

作者： Zhiquan Cao Yixin Bian Tingting Hu Yu yang Zhuolin Cui Tao Wang shuqing yang Xisheng Weng Ruizheng Liang Chaoliang Tan State Key Laboratory of Chemical Resource Engineering Beijing Advanced Innovation Center for Soft Matter Science and EngineeringBeijing University of Chemical TechnologyBeijing100029China Department of Orthopedic Surgery State Key Laboratory of Complex Severe and Rare DiseasesPeking Union Medical College HospitalChinese Academy of Medical Science and Peking Union Medical CollegeBeijing100730China Department of Chemistry and Center of Super-Diamond and Advanced Films(COSDAF) City University of Hong KongKowloon999077Hong KongChina Shenzhen Research Institute City University of Hong KongShenzhen518057China

Over the last decades,bone tissue engineering has increasingly become a research focus in the field of biomedical engineering,in which biomaterials play an important role because they can provide both biomechanical support and osteogenic microenvironment in the process of bone *** these biomaterials,two-dimensional(2D)nanomaterials have recently attracted considerable interest owing to their fantastic physicochemical and biological properties including great biocompatibility,excellent osteogenic capability,large specific surface area,and outstanding drug loading *** this review,we summarize the state-of-the-art advances in 2D nanomaterials for bone tissue ***,we introduce the most explored biomaterials used in bone tissue engineering and their *** then highlight the advances of cutting-edge 2D nanomaterials such as graphene and its derivatives,layered double hydroxides,black phosphorus,transition metal dichalcogenides,montmorillonite,hexagonal boron nitride,graphite phase carbon nitride,and transition metal carbonitrides(MXenes)used in bone tissue ***,the current challenges and future prospects of 2D nanomaterials for bone tissue regeneration in process of clinical translation are discussed.

关键词： 2D nanomaterials Layered double hydroxides Biomaterials Bone tissue engineering Bone repairing/regeneration

Significant annealing-induced hardening effect in nanolaminate d-nanotwinne d(CrCoNi)_(97.4)Al_(0.8)Ti_(1.8)me dium-entropy alloy by severe cold rolling

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Journal of Materials Science & Technology 2024年第3期170卷 156-166页

作者： shuqing Yuan Hui Fu Lei Qian Chi Fai Cheung Xu-Sheng yang State Key Laboratory of Ultra-precision Machining Technology Department of Industrial and Systems EngineeringThe Hong Kong Polytechnic UniversityHung HomKowloonHong KongChina Hong Kong Polytechnic University Shenzhen Research Institute Shenzhen 518060China

Due to the easy coarsening caused by poor thermal stability,the verified annealing-induced hardening in nanograined metals can only maintain at a relatively low-temperature *** this study,a nanolam-inated(CrCoNi)_(97.4)Al_(0.8)Ti_(1.8)medium-entropy alloy with an average lamellae thickness of∼20 nm embedded by thinner nanotwins was fabricated by severe cold rolling to achieve superior thermal *** with the conventional nanotwinned CrCoNi with nanotwins inside ultra-fined grains,the hier-archical nanolaminated-nanotwinned(CrCoNi)_(97.4)Al_(0.8)Ti_(1.8) exhibits a significant annealing-induced hard-ening effect,i.e.,hardness increasing from∼250 HV in the original specimen to∼500 HV in the cold-rolled status and finally∼630 HV after annealing at 600℃for 1 *** microstructure characterizations reveal that the reduced dislocation density and formation of L1_(2)ordered domain are mainly responsible for such hardening effect,which is facilitated by the effectively suppressed coarsening with annealing temperature,i.e.,slow detwinning process and well-retained low-angle nanolamellar *** coarsening mechanisms from the cold-rolled nanolamellae to the fully recrystallized micro-equiaxed structures under the annealing temperatures ranging from 400 to 800℃ were also elucidated by atomic observations.

关键词： Annealing-induced hardening Thermal stability Nanolaminated-nanotwinned structure CrCoNi Detwinning Grain boundary migration

Revealing interfacial charge redistribution of homologous Ru-RuS_(2) heterostructure toward robust hydrogen oxidation reaction

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Journal of Energy Chemistry 2024年第7期94卷 332-339页

作者： Yi Liu Lianrui Cheng shuqing Zhou Yuting yang Chenggong Niu Tayirjan Taylor Isimjan Bao Wang Xiulin yang Guangxi Key Laboratory of Low Carbon Energy Materials School of Chemistry and Pharmaceutical SciencesGuangxi Normal UniversityGuilin 541004GuangxiChina State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China Saudi Arabia Basic Industries Corporation(SABIC)at King Abdullah University of Science and Technology(KAUST) Thuwal 23955-6900Saudi Arabia

Precisely tailoring the surface electronic structures of electrocatalysts for optimal hydrogen binding energy and hydroxide binding energy is vital to improve the sluggish kinetics of hydrogen oxidation reac-tion(HOR).Herein,we employ a partial desulfurization strategy to construct a homologous Ru-RuS_(2) heterostructure anchored on hollow mesoporous carbon nanospheres(Ru-RuS_(2)@C).The disparate work functions of the heterostructure contribute to the spontaneous formation of a unique built-in electric field,accelerating charge transfer and boosting conductivity of ***,Ru-RuS_(2)@C exhibits robust HOR electrocatalytic activity,achieving an exchange current density and mass activity as high as 3.56 mA cm^(-2) and 2.13 mAμg_(Ru)^(-1),*** those of state-of-the-art Pt/C and most contemporary Ru-based HOR ***,Ru-RuS_(2)@C can tolerate 1000 ppm of cO that lacks in Pt/*** analysis reveals that the directional electron transfer across Ru-RuS_(2) heterointerface induces local charge redistribution in interfacial region,which optimizes and balances the adsorption energies of H and OH species,as well as lowers the energy barrier for water formation,thereby promoting theHoR performance.

关键词： Heterostructure Hollow spherical structure Hydrogen oxidation reaction Charge redistribution Density functional calculation

Phytic acid-derivative Co_(2)B-CoPO_x coralloidal structure with delicate boron vacancy for enhanced hydrogen generation from sodium borohydride

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Chinese Chemical Letters 2024年第4期35卷 525-531页

作者： Luyan Shi Ke Zhu Yuting yang Qinrui Liang Qimin Peng shuqing Zhou Tayirjan Taylor Isimjan Xiulin yang Guangxi Key Laboratory of Low Carbon Energy Materials School of Chemistry and Pharmaceutical SciencesGuangxi Normal UniversityGuilin 541004China Saudi Arabia Basic Industries Corporation(SABIC)at King Abdullah University of Science and Technology(KAUST) Thuwal 23955-6900Saudi Arabia

Application of transition metal boride(TMB) catalysts towards hydrolysis of NaBH_(4) holds great significance to help relieve the energy crisis. Herein, we present a facile and versatile metal-organic framework(MOF) assisted strategy to prepare Co_(2)B-CoPO_x with massive boron vacancies by introducing phytic acid(PA) cross-linked Co complexes that are acquired from reaction of PA and ZIF-67 into cobalt boride. The PA etching effectively breaks down the structure of ZIF-67 to create more vacancies, favoring the maximal exposure of active sites and elevation of catalytic activity. Experimental results demonstrate a drastic electronic interaction between Co and the dopant phosphorous(P), thereby the robustly electronegative P induces electron redistribution around the metal species, which facilitates the dissociation of B-H bond and the adsorption of H_(2)O molecules. The vacancy-rich Co_(2)B-CoPO_x catalyst exhibits scalable performance, characterized by a high hydrogen generation rate(HGR) of 7716.7 m L min^(-1)g^(-1) and a low activation energy(Ea) of 44.9 k J/mol, rivaling state-of-the-art catalysts. This work provides valuable insights for the development of advanced catalysts through P doping and boron vacancy engineering and the design of efficient and sustainable energy conversion systems.

关键词： Co_(2)B-CoPO_x catalyst P doping Boron vacancy NaBH_(4) hydrolysis Hydrogen generation