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Synergetic Bioproduction of Short-Chain Fatty Acids from Waste Activated Sludge Intensified by the Combined Use of Potassium Ferrate and Biosurfactants

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Journal of Ocean University of China 2024年第3期23卷 695-709页

作者： CHEN Yanyan yao shuo ZHANG Dahai LI Xianguo FENG Lijuan College of Chemistry and Chemical Engineering Ocean University of ChinaQingdao 266100China

The synergetic effect and underlying mechanism of potassium ferrate(PF)with tea saponin(TS,a biosurfactant)in producing short chain fatty acids(SCFAs)from anaerobic fermentation of waste activated sludge(WAS)were explored in this *** results showed that 0.2 g PF(g TSS)^(-1)(total suspended solid)combined with 0.02 g TS(g TSS)^(-1) could further improve SCFAs’production,and the maximum SCFAs content reached 2008.7 mg COD L^(-1),which is 1.2 and 4.5 times higher than those with PF and TS individually added,respectively,and 5.3 times higher than that of blank WAS on Day *** the model substrates experiments,the degradation rates of bovine serum albumin and dextran with combination of PF and TS were 41.3%±0.1% and 48.5%±0.06%,respectively,on Day 3,which are lower than those in blank WAS(with degradation rates of 72.3%±0.5%and 90.3%±0.3%).It was revealed that the oxidative effect of PF and the solubilization of TS caused more organic matters to be dissolved out from WAS,providing a large number of biodegradable substances for subsequent SCFAs *** WAS pretreated with the combination of PF and TS,the relative abundances of Firmicutes increased from 6.4%(blank)to 38.6%,and that of Proteobacteria decreased from 41.8%(blank)to 21.8%.The combination of PF and TS promoted the hydrolysis process of WAS by enriching Firmicutes,and then increased acetic acid production by inhibiting Proteobacteria that consumed ***,at the genus level,acidogenesis bacteria(e.g.,Proteiniclasticum and Petrimonas)were enriched whereas SCFAs consuming bacteria(e.g.,Dokdonella)were inhibited.

关键词： waste activated sludge anaerobic fermentation potassium ferrate biosurfactant pretreatment

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Magnetized magma intrusions being sources of a weak and a strong lunar magnetic anomaly revealed by 3D distribution of magnetization

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Earth and Planetary Physics 2024年第5期8卷 711-727页

作者： HongYi Wang shuo yao ZeLin Li Liang Hui Guo Jing Yang ChangLi yao Yuan Fang ZhaoJin Rong School of Geophysics and Information Technology Research Center of Lunar and Planetary Remote Sensing Exploration China University of Geosciences (Beijing) Key Laboratory of Intraplate Volcanoes and Earthquakes (China University of Geosciences Beijing) Ministry of Education Hebei University of Engineering Key Laboratory of Earth and Planetary Physics Institute of Geology and Geophysics Chinese Academy of Sciences

In this work, we aim to investigate the origin of the magnetic carriers in the lunar crust and the intensity of the ancient dynamo field. The magnetization and depth range of magnetic carriers are studied under a weak and a strong magnetic anomaly in Mare Tranquillitatis and in Oceanus Procellarum, respectively, where the surface ages are 3.6 and 3.3 billion years. A sophisticated threedimensional amplitude inversion software program from a geophysical survey is used to reconstruct the distributions of magnetization in the lunar crust. Because no globally measured surface magnetic field exists for the Moon, a crustal magnetic anomaly model with a grid resolution of 0.2° is used. The depth range of the magnetic source is fixed by the boundary identified by a relative criterion, which is 20%of the recovered maximum magnetization. The central burial depths of the magnetic carriers are approximately 15 km and 25 km under Reiner Gamma and Mare Tranquillitatis, respectively. The volumes of the two magnetic sources are at scales of 104 and 105 km3,respectively. The aforementioned differences may imply a hotter crust under Reiner Gamma than Mare Tranquillitatis by 3.3 billion *** results support the view that the magma intrusions magnetized by an ancient magnetic field could be the origin of magnetic anomalies under Reiner Gamma and Mare Tranquillitatis. Compared with previous works, the maximum magnetization of 3 A/m under Reiner Gamma supports the intensity of the field being several microteslas.

关键词： lunar magnetic anomaly 3D distribution of magnetization Reiner Gamma Mare Tranquillitatis

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Illuminating spin-crossover octanuclear metal-organic cages

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Science China Chemistry 2024年第4期67卷 1208-1215页

作者： Zhi-Kun Liu Alyona A.Starikova Yu-Xia Li Ke Sun Meng Yu Zi-shuo yao Jun Tao Key Laboratory of Cluster Science of Ministry of Education School of Chemistry and Chemical EngineeringLiangxiang CampusBeijing Institute of TechnologyBeijing 102488China Institute of Physical and Organic Chemistry Southern Federal UniversityRostov-on-Don 344090Russia

Spin-crossover(SCO)materials that reversibly switch between high-and low-spin states have potential for the storage of spin state-relative information,and have gained much attention incorporating secondary physical properties,such as fluorescence and magneto-optical *** this study,we synthesized three octanuclear metal-organic cages(MOCs)using tetraphenylethylene-based luminophores,aldehydes,and Fe^(Ⅱ)salts,by subcomponent self-assembly approach,namely[Fe1]-[Fe3].By controlling the ligand-field strength and guest encapsulation,we finely tuned their SCO *** them,MOC[Fe2]displayed nearly complete SCO behavior in the solid state,which is rare for high-nuclearity *** also demonstrated the coupling of SCO with fluorescence emission in these MOCs by using isostructural Zn^(Ⅱ)complexes([Zn1]-[Zn3])as control experiments,for the first *** calculations revealed the energy-transfer mechanism between fluorophores and SCOactive centers,which emphasizes the significant contribution of d-d transitions in the interplay between the occurrence of SCO and fluorescence emission.

关键词： metal-organic cages spin crossover fluorescence switching materials DFT calculation

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Characteristics and applications of interplanetary coronal mass ejection composition

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Science China(Technological Sciences) 2020年第11期63卷 2171-2187页

作者： SONG HongQiang yao shuo Shandong Provincial Key Laboratory of Optical Astronomy and Solar-Terrestrial Environment Institute of Space SciencesShandong UniversityWeihai 264209China School of Geophysics and Information Technology China University of Geosciences(Beijing)Beijing 100083China

In situ measurements of interplanetary coronal mass ejection(ICME)composition,including elemental abundances and charge states of heavy ions,open a new avenue to study coronal mass ejections(CMEs)besides remote-sensing *** ratios between different elemental abundances can diagnose the plasma origin of CMEs(e.g.,from the corona or chromosphere/photosphere)due to the first ionization potential(FIP)effect,which means elements with different FIPs get fractionated between the photosphere and *** ratios between different charge states of a specific element can provide the electron temperature of CMEs in the corona due to the freeze-in effect,which can be used to investigate their eruption *** this review,we first give an overview of the ICME composition and then demonstrate their applications in investigating some important subjects related to CMEs,such as the origin of filament plasma and the eruption process of magnetic flux ***,we point out several important questions that should be addressed further for better utilizing the ICME composition to study CMEs.

关键词： coronal mass ejection interplanetary coronal mass ejection elemental abundance ionic charge state magnetic flux rope magnetic cloud filament flare

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淮南深地地磁总场连续观测与时变特征

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地球物理学报 2024年第2期67卷 820-828页

作者：郭良辉王博宋晓林王赟金承奕姚硕史钰峰陆内火山与地震教育部重点实验室(中国地质大学北京)北京100083 中国地质大学(北京)地球物理与信息技术学院北京100083 地质过程与矿产资源国家重点实验室(中国地质大学) 北京100083 山东航天电子技术研究所山东烟台264000

深地实验室具有电磁干扰小的"超净"地磁观测优势,已成为地磁观测的新平台和研究热点.为了评估淮南深地实验室地磁环境、认识地磁总场时变特征及地下与地面耦合性,我们于2022年在地下巷道(-848 m)和地表(+22 m)同步实施了地磁总场长期连... 详细信息

深地实验室具有电磁干扰小的"超净"地磁观测优势,已成为地磁观测的新平台和研究热点.为了评估淮南深地实验室地磁环境、认识地磁总场时变特征及地下与地面耦合性,我们于2022年在地下巷道(-848 m)和地表(+22 m)同步实施了地磁总场长期连续观测,对观测数据进行了功率谱、小波谱、地磁总场变化特征的分析及与附近的蒙城地磁台比对.研究结果表明:(1)地下无(或弱)磁干扰点位的地磁环境较优越,与蒙城地磁台相媲美,适用于地磁场变化的高精度、长期连续观测;(2)地下地磁观测可有效过滤地表上由工业与人类活动等的电磁干扰,其记录的地磁总场变化与蒙城地磁台具有较好的耦合性;(3)地下磁测可清晰记录地磁总场的平静变化、不同周期磁暴、地磁脉冲等丰富的时变信息,可为地球系统科学研究提供地磁学依据.

关键词：深地实验室地磁总场时间变化观测地磁环境

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肉桂醛调节AMPK/SREBP1c信号通路对非酒精性脂肪性肝炎小鼠肝损伤的影响

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广州中医药大学学报 2024年第2期41卷 438-447页

作者：姚硕李源段超曾艳华中科技大学同济医学院附属梨园医院中西医结合科湖北武汉430077 华中科技大学同济医学院附属梨园医院血液科湖北武汉430077

【目的】探讨肉桂醛通过调节腺苷酸活化蛋白激酶(AMPK)/胆固醇调节元件结合蛋白1c(SREBP1c)信号通路对非酒精性脂肪性肝炎(NASH)小鼠肝损伤的影响。【方法】实验随机分为正常组,模型组,肉桂醛低、中、高剂量组及肉桂醛高剂量+Compound C... 详细信息

【目的】探讨肉桂醛通过调节腺苷酸活化蛋白激酶(AMPK)/胆固醇调节元件结合蛋白1c(SREBP1c)信号通路对非酒精性脂肪性肝炎(NASH)小鼠肝损伤的影响。【方法】实验随机分为正常组,模型组,肉桂醛低、中、高剂量组及肉桂醛高剂量+Compound C(AMPK抑制剂)组,每组15只小鼠。除正常组,其他各组给予高脂饲料喂养构建NASH模型。干预治疗后,观察肝组织病理形态变化,检测血清生化指标[丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、甘油三酯(TG)、总胆固醇(TC)],肝组织氧化应激指标[丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽(GSH)],AMPK/SREBP1c通路蛋白[磷酸化AMPK(p-AMPK)、AMPK、SREBP1c、乙酰辅酶A羧化酶(ACC)、磷酸化ACC(p-ACC)、肉毒碱棕榈酰转移酶1(CPT1)、硬脂酰辅酶A去饱和酶1(Scd1)]表达,及CPT1α、长链酯酰辅酶A脱氢酶(Lcad)、ACC1、脂肪酸合成酶(FAS)的mRNA表达水平。【结果】与正常组比较,模型组肝脏脂肪变性,脂质沉积和纤维化间质增多,ALT、AST、TG、TC,MDA含量,SREBP1c、Scd1蛋白表达,ACC1、FAS mRNA表达水平显著升高,SOD、GSH活性,p-AMPK、AMPK、p-ACC、ACC、CPT1蛋白表达,CPT1α、Lcad m RNA表达水平显著降低(P<0.05);与模型组比较,肉桂醛低、中、高剂量组脂质沉积和纤维化间质减少,ALT、AST、TG、TC、MDA含量,SREBP1c、Scd1蛋白表达,ACC1、FAS mRNA表达水平显著降低,SOD、GSH活性,p-AMPK、AMPK、p-ACC、ACC、CPT1蛋白表达,CPT1α、Lcad mRNA表达水平显著升高(P<0.05),且呈剂量依赖性;与肉桂醛高剂量组比较,肉桂醛高剂量+Compound C组上述指标均被逆转(P<0.05)。【结论】肉桂醛可能通过调控AMPK/SREBP1c通路,抑制氧化应激,改善肝脏脂肪变性,减轻NASH小鼠肝损伤。

关键词：肉桂醛非酒精性脂肪性肝炎肝损伤腺苷酸活化蛋白激酶(AMPK) 胆固醇调节元件结合蛋白1c(SREBP1c) 小鼠

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柴胡桂枝干姜汤通过RIP1/RIP3/MLKL通路抑制坏死性凋亡改善大鼠非酒精性脂肪肝

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广州中医药大学学报 2024年第4期41卷 988-994页

作者：李源姚硕段超曾艳华中科技大学同济医学院附属梨园医院中西医结合科湖北武汉420081 华中科技大学同济医学院附属梨园医院血液科湖北武汉430077

【目的】探讨柴胡桂枝干姜汤对非酒精性脂肪肝(NAFLD)大鼠的治疗作用及机制。【方法】实验设正常组,模型组,中药(柴胡桂枝干姜汤)低、高剂量组及GSK872[受体相互作用蛋白激酶(RIP)3抑制剂]组等5组。除正常组,其余各组大鼠分别采用高脂... 详细信息

【目的】探讨柴胡桂枝干姜汤对非酒精性脂肪肝(NAFLD)大鼠的治疗作用及机制。【方法】实验设正常组,模型组,中药(柴胡桂枝干姜汤)低、高剂量组及GSK872[受体相互作用蛋白激酶(RIP)3抑制剂]组等5组。除正常组,其余各组大鼠分别采用高脂饲料饲喂法构建NAFLD模型。治疗结束后,采用原位末端转移酶标记(TUNEL)法观察肝细胞凋亡情况,酶联免疫吸附分析(ELISA)检测中血清中谷丙转氨酶(ALT)、谷草转氨酶(AST),血脂[总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)]水平及肝组织中炎症因子肿瘤坏死因子α(TNF-α)、白细胞介素1β(IL-1β)水平,Western Blot法检测肝组织中RIP1、RIP3、混合谱系激酶结构域样蛋白(MLKL)磷酸化水平。【结果】与正常组比较,模型组大鼠肝细胞凋亡指数升高,血清中ALT、AST及TC、TG、LDL-C水平显著升高,HDL-C水平显著降低,肝组织中TNF-α、IL-1β含量及RIP1、RIP3、MLKL磷酸化表达水平均显著升高(P0.05)。【结论】柴胡桂枝干姜汤可以有效改善大鼠NAFLD,其机制可能与抑制RIP1/RIP3/MLKL信号通路活化,进而抑制坏死性凋亡有关。

关键词：柴胡桂枝干姜汤非酒精性脂肪肝坏死性凋亡 RIP1/RIP3/MLKL通路大鼠

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Synergetic Bioproduction of Short-Chain Fatty Acids from Waste Activated Sludge Intensified by the Combined Use of Potassium Ferrate and Biosurfactants

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中国海洋大学学报（自然科学英文版） 2024年第3期 695页

作者： CHEN Yanyan yao shuo ZHANG Dahai LI Xianguo FENG Lijuan

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Effects of Potassium Ferrate and Low-Temperature Thermal Hydrolysis Co-Pretreatment on the Hydrolysis and Anaerobic Digestion Process of Waste Activated Sludge

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Journal of Ocean University of China 2023年第6期22卷 1583-1591页

作者： MA Yingpeng HAO Di yao shuo ZHANG Dahai LI Xianguo FENG Lijuan Key Laboratory of Marine Chemistry Theory and Technology Ministry of EducationCollege of Chemistry and Chemical EngineeringOcean University of ChinaQingdao 266100China

This study evaluated the effect of potassium ferrate(PF)and low-temperature thermal hydrolysis co-pretreatment on the promotion of sludge hydrolysis process and the impact on acid production in the subsequent anaerobic digestion *** analytical investigations showed that co-pretreatment significantly facilitated the hydrolysis process of the sludge and contributed to the accumulation of short-chain fatty acids(SCFAs).The pretreatment conditions under the optimal leaching of organic matter from sludge were hydrothermal temperature of 75℃,hydrothermal treatment time of 12 h,and PF dosage of 0.25 g g^(−1)TSS(total suspended solids),according to the results of orthogonal *** pretreatment under proper conditions,the removal rate of soluble chemical oxygen demand(SCOD)achieved 71.8%at the end of fermentation and the removal rate of total phosphorus(TP)was 69.1%.The maximum yield of SCFAs was 750.3 mg L^(−1),7.45 times greater than that of the blank *** on the analysis of the anaerobic digestion mechanism,it was indicated that the co-pretreatment could destroy the floc structure on the sludge surface and improve organic matter dissolving,resulting in more soluble organic substances for the acidification ***,microbial community research revealed that the main cause of enhanced SCFAs generation was an increase in acidogenic bacteria and a reduction of methanogenic bacteria.

关键词： waste activated sludge potassium ferrate low-temperature thermal hydrolysis anaerobic digestion short-chain fatty acids

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贝氏体等温时间对两相区轧制舰船用钢组织和性能的影响

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材料热处理学报 2024年第7期45卷 147-153页

作者：田亚强姚硕张明山李红斌胡宝佳陈连生华北理工大学现代冶金技术教育部重点试验室河北唐山063210

采用扫描电镜(SEM)、X射线衍射仪(XRD)和电子万能试验机等研究了两相区轧制后贝氏体等温处理时间对舰船用钢组织性能的影响。结果表明:当在450℃等温处理10 min到1 h时,试验钢的显微组织由马奥岛(M/A岛)、少量粒状贝氏体和铁素体组成;... 详细信息

采用扫描电镜(SEM)、X射线衍射仪(XRD)和电子万能试验机等研究了两相区轧制后贝氏体等温处理时间对舰船用钢组织性能的影响。结果表明:当在450℃等温处理10 min到1 h时,试验钢的显微组织由马奥岛(M/A岛)、少量粒状贝氏体和铁素体组成;随着等温时间的延长,M/A岛逐渐减少,粒状贝氏体增多;当等温时间达到5 h时,显微组织由粒状贝氏体和少量铁素体组成。随着等温时间的继续延长,粒状贝氏体不断长大,残留奥氏体分解为碳化物,试验钢的屈服强度为1116~1150 MPa,抗拉强度为1121~1156 MPa。随着等温时间的延长,试验钢的断后伸长率和冲击吸收能量均呈先升高后降低趋势,在等温5 h时,伸长率和冲击吸收能量均达到最大值,分别为15.6%和81.2 J。

关键词：两相区轧制贝氏体等温时间舰船用钢粒状贝氏体冲击吸收能量

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