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Expression and Characterization of an Active Chimeric Protein of Human Extracellular Superoxide Dismutase and hCuZnSOD in Pichia pastoris

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Chemical Research in Chinese Universities 2010年第2期26卷 240-244页

作者： qu He-zhi DU Shan-shan YANG shuo HUANG Lu ZHANG Lei XIAO Song HAO Dong-yun WANG Xiao-ping Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education Jilin Unversity Changchun 130021 P. R. China Biotechnology Research Centre Jilin Academy of Agricultural Science Changchun 130033 P. R. China

Mammalian cells express two isoforms of Cu- and Zn-containing superoxide dismutases（SODs）, CuZn-SOD and extracellular SOD（EC-SOD）, involved in the defense system against reactive oxygen species（ROS）. The two SODs have structurally homologous centre domain with distinct N- and C-terminuses, resulting in the different characteristics of the structure and function of the two molecules. We generated a hybrid SOD molecule（namely hy- SOD） via replacing the N- and C-terminuses of hCuZnSOD with the counterparts of hEC-SOD. The hySOD was expressed in host Pichia pastoris and the purified protein was a dimer with a molecular weight of about 34000. A series of activity analyses indicates that the hySOD is similar to hEC-SOD in heat-stability, and has the activity of protecting the host cell against heat shock and oxidative stress. Our results show evidence for the study on the compound activity of multiple SOD molecules, and may be important for understanding the relationship between structure and function of hEC-SOD and hCuZnSOD.

关键词： Extracellular superoxide dismutase Protein fusion Heterologous expression Pichiapastoris

Additive manufacturing of high-strength CrMnFeCoNi high-entropy alloys-based composites with WC addition

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Journal of Materials Science & Technology 2019年第11期35卷 2430-2434页

作者： Jinfeng Li shuo Xiang Hengwei Luan Abdukadir Amar Xue Liu Siyuan Lu Yangyang Zeng Guomin Le Xiaoying Wang Fengsheng qu Chunli Jiang Guannan Yang Institute of Materials China Academy of Engineering PhysicsMianyang 621907China College of Physics and Technology Xinjiang UniversityUrumqiXinjiang 830046China School of Materials Science and Engineering Tsinghua UniversityBeijing 100084China School of Mechanical Engineering and Mechanics Ningbo UniversityNingbo 315211China National key laboratory of shock wave and detonation physics Institute of Fluid PhysicsChina Academy of Engineering PhysicsMianyang 621907China School of Electromechanical Engineering Guangdong University of TechnologyGuangzhou 510006China

Laser melting deposition with WC addition has been developed to fabricate high-strength CrMnFeCoNibased high-entropy alloys-based *** this technique,a microstructure of compact refined equiaxed grains can be achieved,and the tensile strength can be remarkably *** sample with 5 wt%WC addition shows a promising mechanical performance with a tensile strength of 800 MPa and an elongation of 37%.The improvement in mechanical property may be attributed to the formation of Cr(23)C6 reinforcement precipitates,which could promote the heterogeneous nucleation of grains and hinder the propagation of slip bands.

关键词： High-entropy alloys Laser metal deposition Precipitates Microstructures Tensile test

Distinctive prognostic value of Ki-67 in different genotype of NSCLC patients

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Distinctive prognostic value of Ki-67 in different genotype ...

乌司他丁对放射性肺损伤模型大鼠干预作用及层黏连蛋白和羟脯氨酸的影响

2018年中国肿瘤标志物学术大会暨第十二届肿瘤标志物青年科学家论坛

作者： Jinfeng Guo shuo Wang Xiujuan qu Department of Medical Oncology the First Hospital of China Medical University Key Laboratory of Anticancer Drugs and Biotherapy of LiaoNing Province the First Hospital of China Medical University

Objective : To investigate the association of Ki-67 with pathological characteristics and prognostic value in different genotype of NSCLC patients. Methods :347 cases of unresected local advanced or metastatic NCSCL patients were selected from the first affiliated hospital of China Medical University. Data about pathological features and survival were collected respectively. Genetic test results were achieved from plasma samples or tumor tissue samples by high throughput sequencing and Ki-67 proliferation index were analyzed by immunohistochemistry retrospectively. The relationship between Ki-67 and pathological features(such as gender, age, pathological type, tumor size, lymphatic metastasis, TMM stage, ECOG score and different gene status) was examined by Spearman’s rank correlation. Survival curves were calculated by the Kaplan-Meier *** analyses were performed using the Cox regression model to study factors that independently influenced overall survival. Pquamous cell cancer vs 51% in non-squamous cell cancer, P<0.001), and EGFR gene status(60% in wild type vs 48% in EGFR gene mutant type, P=0.037) in NSCLC *** survival analysis, low Ki-67 expression indicated better survival for PFS and OS in EGFR wild type(mPFS:6.0 months vs 5.0 months, P=0.004;mO S: not reached vs 27.0 months, P=0.020) 、 EGFR mutant type(mPFS:12.0 months vs 7.0 months, P<0.001;mO S: not reached vs 48.0 months, P=0.002),KRAS gene wild type(mPFS:10.0 months vs 5.0 months, P=0.024;mO S:53.0 months vs 24.0 months,P=0.031), PIK3 CA gene wild type(mPFS:7.0 months vs 5.0 months, P=0.015;mO S:not reached vs19.5 months, P=0.007) and PTEN gene wild type(mPFS:11.0 months vs 6.0 months, P=0.005;mO S:not reached vs 40.0 months, P=0.020) groups Furthermore, Ki-67 expression can also play as a great predictive

关键词： NSCLC Ki-67 genotype prognosis PFS OS

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国际呼吸杂志 2008年第22期28卷 1345-1348页

作者：保鹏涛戚好文李树钧杨善潮张力江屈朔瑶吴昌归高维第四军医大学西京医院呼吸内科西安710033

目的探讨乌司他丁对放射性肺损伤的治疗作用及层黏连蛋白（LN）和羟脯氨酸（Hyp）在放射性肺损伤中的变化。方法雌性SD大鼠100只随机分为3组：正常对照组（C组）24只，单纯照射组（R组）40只，乌司他丁治疗组（U组）36只。R组及U组动物... 详细信息

目的探讨乌司他丁对放射性肺损伤的治疗作用及层黏连蛋白（LN）和羟脯氨酸（Hyp）在放射性肺损伤中的变化。方法雌性SD大鼠100只随机分为3组：正常对照组（C组）24只，单纯照射组（R组）40只，乌司他丁治疗组（U组）36只。R组及U组动物麻醉后，行直线加速器全胸部照射一次，剂量为25Gy。U组照射后即按乌司他丁100000U／kg，尾静脉注射，而后每天以相同剂量尾静脉注射7d。C组和R组尾静脉注射等体积生理盐水。于照射后第7、15、30、60、90、150天处死动物，取部分肺组织行HE染色及Masson染色，观察组织学及肺胶原纤维等，使用放射免疫法检测血清中LN水平，碱水解法测定血清中Hyp的含量。结果U组大鼠生存率（88．9％）明显高于R组大鼠（62．5％）。R组大鼠肺部在照射后，早期肺泡明显出现充血和水肿，晚期部分肺泡腔塌陷，可见成纤维细胞构成的成纤维病灶，甚至大面积的肺完全实变；与R组相比，U组中、晚期充血、水肿及肺间隔增宽不明显，少见纤维化病灶及实变。R组血清中LN、Hyp水平随着照射时间逐渐增加，在150d时达到高峰，分别为（129．70±3．48）ng／ml、（193．70±5．41）ng／ml，这与肺部组织学变化趋势基本一致，U组与R组相比却明显减少（P〈0．01）。结论乌司他丁能有效治疗放射性肺损伤，为放射性肺损伤的防治提供了一种新的方法。

关键词：层黏连蛋白羟脯氨酸乌司他丁肺放射

Experimental study on the gas-stripping chamber of an E//B neutral particle analyzer

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Nuclear Science and Techniques 2024年第11期35卷 98-107页

作者： Long Ma De-Hao Xie Yan-Xi Wang shuo Wang Ji-Feng Han Wei-Ping Lin Jian-Li Liu Xing-quan Liu Yu-Guo Liu Xiao-Bing Luo Yuan Luo Guo-Feng qu Yu-Fan qu Pei-Pei Ren Roy Wada Lin-Ge Zang Jing-Jun Zhu Key Laboratory of Radiation Physics and Technology of the Ministry of Education Institute of Nuclear Science and TechnologySichuan UniversityChengdu 610064China Institute of Modern Physics Chinese Academy of SciencesLanzhou 730000China Southwestern Institute of Physics Chengdu 610218China Cyclotron Institute Texas A&M UniversityCollege StationTX 77843USA

Stripping units take a key role in the neutral particle analyzer(NPA).A renovated gas-stripping unit was constructed for the newly designed E//B *** H_(2)as the working gas,we measured the gas inlet pressure(P_(0))and vacuum chamber pressure(P_(3)).The pressure distribution inside the gas-stripping room was calculated with Ansys Fluent,using the measured P_(0)and P_(3)as boundary *** stripping efficiency of the stripping unit was then simulated utilizing the Geant4 Monte Carlo code for the H and D *** pressure P_(0)=40 Pa,which is one-sixth of what found in the previous design and corresponds to a thickness of 1.27×10^(17)atoms∕cm^(2),was obtained as the optimum working pressure for the upgraded stripping *** 50 kV electron cyclotron resonance(ECR)ion source platform was designed and constructed for E//B NPA calibration,and its performance has been *** the ECR ion source platform,we measured the efficiency of the stripping unit through an inverse experiment with proton *** compared the current ratios of measurements with and without H_(2)gas to Geant4 simulation *** found adequate agreement between the overall trends of the experiment and the *** significant deviation for incident energies below 20 keV may result from the scattering effects of low-energy protons,leading to reduced accuracy in single-scattering physics in Geant4 *** the scattering corrections observed in the reverse experiments obtains more accurate stripping efficiencies for H and D atoms in the energy range of 20–200 keV and the global efficiency with the maximum values of 95.0%for H atoms and 78.9%for D atoms at 200 keV.

关键词： NPA Gas-stripping ECR ion source Stripping efficiency Geant4

Contemporary Asymmetric Genetic Introgression Between Two Pelophylax Species in Shanghai

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Asian Herpetological Research 2024年第1期15卷 1-11页

作者： Xu WEI Wenli LIU Gang WANG Rui MA Meiling HUANG Jiaxin ZHENG Tingwei DONG Changqing YUAN Shunqi BO Xiao YUAN Ben LI shuo MA qu YUE Youzhong DING Zhenghuan WANG School of Life Sciences East China Normal UniversityShanghai 200241China Shanghai Landscaping and City Appearance Administrative Bureau Shanghai 200041China State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 200241China Shanghai Key Lab for Urban Ecological Processes and Eco-Restoration East China Normal UniversityShanghai 200241China Yangtze Delta Estuarine Wetland Ecosystem Observation and Research Station Ministry of Education&Shanghai Science and Technology CommitteeShanghai 202162China

Human disturbances are considered to break reproduction barriers among *** increases in hybridization events have been reported among a large number of taxonomic groups in anthropogenic environments,providing novel insights into species evolution mechanisms and conservation management in the *** Eastern Golden Frog(Pelophylax plancyi)and BlackSpotted Frog(***)are two sympatric anuran species with a long history of mitochondrial genome introgression in highly urbanized continental East ***,there is only limited understanding of the pattern of their contemporary hybridization and factors influencing their interspecific relationship under anthropogenic ***,interspecific hybridization between *** and *** at the population level was investigated in *** except two haplotypes obtained from both species in Shanghai were mixed together,and located in the introgression clade,implying multiple ancient mitochondrial introgression events occurred in the populations of our study *** genetic introgression was detected by microsatellite markers,with 0.7%of *** and 14.6%of *** identified as contemporary admixed *** with the trend of population density,higher genetic diversity of neutral microsatellite loci was found in the more abundant ***;however,variation in mitochondrial(Cyt-b)and nuclear(POMC)genes was higher in relatively rare *** population density of *** and number of water patches within local habitats were significantly positively correlated with both occurrences and proportions of admixed individuals in the populations of *** and *** the prevalent transformation of habitats in urbanized areas,these results imply that a high population density in isolated artificially altered habitats is likely to increase interspecific ***,population monitoring and improveme

关键词： anthropogenic disturbances interspecific hybridization Pelophylax species abundance urban landscape urbanization

Transitioning from heterogeneous VSC to homogeneous VSC based power systems:Leveraging dual-port grid-forming VSCs

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iEnergy

iEnergy 2024年第2期3卷 75-76页

作者： shuo Zhang Wei Qiao Liyan qu Jun Wang Department of Electrical and Computer Engineering University of Nebraska-LincolnLincolnNE 68588-0511USA

Original reference:iEnergy,3(1):46-58,2024 Grid-tie voltage source converters(VSCs)can operate in three distinct modes:AC-dominant,DC-dominant,and balanced,depending on the placement of the stiff voltage sources,as shown in Figure *** distinct operation modes of VSCs typically require different synchronization control *** instance,the grid-following(GFL)control,which utilizes a phase-locked loop to track the AC grid phase and frequency,can be employed for VSCs operating in the AC-dominant mode and the balanced *** the other hand,the grid-forming(GFM)control is uti-lized for VSCs operating in the DC-dominant mode and the bal-anced ***,neither GFM control nor GFL control can serve as a universal synchronization control technique for VSCs to operate in all of the three *** the combination of the GFL VSCs and the GFM VSCs can handle applications that require the VSCs to operate in all of the three modes,effectively accommodating and coordinating the heterogeneous GFL and GFM VSCs remains challenging for power systems.

关键词： utilize Figure Transition

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后脱欧时代“全球英国”外交战略及其前景

现代国际关系 2021年第1期 21-28,63,64页

作者：曲兵王朔中国现代国际关系研究院欧洲研究所

英国约翰逊政府将"全球英国"作为后脱欧时代对外政策的目标,宣称要利用重新获得的自主权,在国际舞台上积极作为,维系英国具有全球影响力的大国地位。然而在实践中,"全球英国"面临诸多挑战,甚至陷入左右为难的选择困境。其原因有多个方面... 详细信息

英国约翰逊政府将"全球英国"作为后脱欧时代对外政策的目标,宣称要利用重新获得的自主权,在国际舞台上积极作为,维系英国具有全球影响力的大国地位。然而在实践中,"全球英国"面临诸多挑战,甚至陷入左右为难的选择困境。其原因有多个方面,主要是"全球英国"构想存在内在缺陷,英国内部政治嬗变削弱了对外政策的弹性。当然,英国仍试图在外交上有所作为,尤其是维持某种程度的战略自主,因而仍将是国际格局中的一个重要变量。

关键词： “全球英国” 脱欧英国外交

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苯系物作为唯一碳源对异化铁还原过程的影响

西北农林科技大学学报（自然科学版） 2006年第10期34卷 101-106,112页

作者：刘硕曲东西北农林科技大学资源环境学院陕西杨凌712100

以水稻土浸提液为接种液、人工合成的无定形氧化铁为唯一的电子受体、添加的苯系物(甲苯、苯胺、苯酚、苯甲酸)为唯一碳源,在适量无机N,P,K营养供给条件下,采用厌氧恒温混合培养方法,研究了厌氧微生物对氧化铁还原的影响。结果表明,由... 详细信息

以水稻土浸提液为接种液、人工合成的无定形氧化铁为唯一的电子受体、添加的苯系物(甲苯、苯胺、苯酚、苯甲酸)为唯一碳源,在适量无机N,P,K营养供给条件下,采用厌氧恒温混合培养方法,研究了厌氧微生物对氧化铁还原的影响。结果表明,由水稻土中提取的微生物可以利用苯系物作为唯一碳源,在厌氧降解的同时将电子转移给F e(Ⅲ),使其还原为F e(Ⅱ);利用不同苯系物的铁还原过程难易程度有所差异,铁还原率表现为苯甲酸最高(53.99%),甲苯最低(19.55%),利用苯系物的铁还原率均低于以葡萄糖为碳源时的铁还原率(71.16%);不同浓度苯系物对氧化铁还原过程有不同程度的影响,在一定浓度范围内,随着苯系物浓度的增加其对氧化铁还原过程具有促进作用,而高浓度苯系物则对氧化铁还原过程表现出抑制趋势;以2.0 mm o l/L苯系物作为碳源时,蒽醌-2,6二-磺酸盐(AQDS)可明显增加F e(Ⅱ)的累积量。

关键词：异化铁还原苯系物水稻土蒽醌-2,6-二磺酸盐