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Correlation Analysis of New Soybean[Glycine max(L.)Merr]Gene Gm15G117700 with Oleic Acid

Phyton-International Journal of Experimental Botany 2021年第4期90卷 1177-1192页

作者： shuo qu Yaolei Jiao Lamboro Abraham Piwu Wang Plant Biotechnology Center Jilin Agricultural UniversityChangchun130018China College of Agronomy Jilin Agricultural UniversityChangchun130018China

The fatty acid dehydrogenase gene plays an important role in regulating the oleic acid content in ***-wide association study screened out soybean oleic acid related gene Gm15G117700.A fragment size of 693bp was obtained by PCR amplification of the gene and,it was connected by seamless cloning technology to the pMD18T cloning *** on the gene sequence cloned,bioinformatic analysis of gene protein was *** overexpression vector of Gm15G117700 and the CRISPR/Cas9 gene editing vector were *** positive plants were obtained by Agrobacterium-mediated transformation of soybean cotyledon nodes and T2 plants were identified by conventional PCR,QT-PCR and Southern blot hybridization.10 copies of high and low oleic acid seeds were selected for QT-PCR to identify the expression content of Gm15G117700 gene in different soybeans,and finally near-infrared spectroscopy analyzer was used to identify the oleic acid quality of soybeans.T2 RT-PCR identification showed that overexpression was reduced by 3.94%,and gene editing was increased by 3.49%.It is determined that the Gm15G117700 gene may belong to a regulatory gene,a minor gene that can promote the conversion to linoleic acid content in soybean oleic acid *** gene cloning and its functional verification was not reported *** is the first report by PCR amplification of soybean Gm15G117700 genes and gene expression *** the content of oleic acid in soybean lay a foundation for ***;this study clearly identified the function of soybean Gm15G117700 gene and its role played in oleic acid synthesis and metabolism.

关键词： Soybean Gm15G117700 genes cloning expression vector

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Bioinformatics Analysis of Disease Resistance Gene PR1 and Its Genetic Transformation in Soybeans and Cultivation of Multi-resistant Materials

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1445-1464页

作者： Huimin Cui shuo qu Abraham Lamboro Yaolei Jiao Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun1300118China

In agricultural production,a single insect-resistant and disease-resistant variety can no longer meet the *** this study,the expression vector pCAMBIA-3301-PR1 containing the disease-resistant gene PR1 was constructed by means of genetic engineering,and the PR1 gene was genetically transformed to contain the PR1 gene through the pollen tube *** CryAb-8Like transgenic high-generation T7 receptor soybean,a new material that is resistant to insects and diseases is *** T2 transformed plants,routine PCR detection,Southern Blot hybridization,fluorescence quantitative PCR detection,indoor and outdoor pest resistance identification and indoor disease resistance identification were *** results showed that there were 9 positive plants in the routine PCR test of T2 *** Southern Blot hybridization,both PR1 and CryAb-8Like genes are integrated in soybeans in the form of single *** quantitative PCR showed that the expression levels of PR1 and CryAb-8Like genes are different in different *** average expression levels of PR1 gene in plant roots,stems,and leaves are 2.88,1.54,and 5.26,***-8Like genes are found in roots,stems,and *** average expression levels were 1.36,1.39,and 4.25,*** insectivorous rate of the CryAb-8Like gene in outdoor plants with positive insect resistance identification was 3.78%.The disc partition method was used indoors for pest resistance identification,and the bud length of transformed plants increased *** average mortality rate of untransformed plants in indoor disease resistance identification was as high as 56.66%,and the average mortality rate of plants transformed with PR1 gene was 10.00%,and disease resistance was significantly ***,a new material with resistance to diseases and insects is obtained.

关键词： Soybean PR1 gene CryAb-8Like gene insect resistance disease resistance

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Hierarchical crystalline-amorphous nanocomposites with high strength and large deformability enabled by elemental diffusion

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Journal of Materials Science & Technology 2024年第4期171卷 150-161页

作者： Liqiang Wang Heyi Wang Xin Zhou Huangliu Fu James Utama Surjadi shuo qu Xu Song Rong Fan Yang Lu Department of Mechanical Engineering City University of Hong KongHong KongChina Nano-Manufacturing Laboratory(NML) Shenzhen Research Institute of City University of Hong KongShenzhen 518057China Chengdu Research Institute City University of Hong KongChengdu 610200China Shenyang National Laboratory for Materials Science Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China Department of Mechanical and Automation Engineering The Chinese University of Hong KongHong KongChina Department of Mechanical Engineering The University of Hong KongHong KongChina

Amorphous/nanocrystalline dual-phase structures have recently emerged as an effective way for over-coming the strength-ductility trade-offand breaking the limitation of the reverse Hall-Petch ***,we proposed a new strategy to develop a hierarchical and interconnected amorphous-crystalline nanocomposite arising from the nanoscale elemental interdiffusion and oxygen adsorption behavior dur-ing thermal treatment *** nanocomposite consisted of a three-dimensional(3D)hierarchical network structure where the crystalline phase(Cr-Co-Ni-Al)was embedded into the Al-O-based amor-phous phase network with critical feature sizes encompassing three orders of magnitude(from microm-eter to nanometer scale).It can achieve ultrahigh compression yield strength of-3.6 GPa with large homogeneous deformation of over 50%*** massive interstitial atoms induced lattice distortion and hierarchical amorphous phase boundary contributed to the strength *** situ Uniaxial compression inside a transmission electron microscope(TEM)revealed that the exceptional deformability of the nanocomposites resulted from the homogenous plastic flow of nano-sized amorphous phase and the plastic co-deformation behavior restricted by the nano-architected dual-phase *** proposed dual-phase synthesis approach can outperform conventional nanolaminates design strategies in terms of the mechanical properties achievable while providing a pathway to easily tune the microstructure of these nanolaminates.

关键词： Nanolaminates Dual-phase nanocomposites In situ transmission electron microscopy Multi-component alloy

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Bioinformatics and Functional Analysis of High Oleic Acid-Related Gene GmSAM22 in Soybean [Glycine max (L.) Merr.]

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Phyton-International Journal of Experimental Botany 2023年第2期92卷 501-519页

作者： shuo qu Qi Cai Huimin Cui Lamboro Abraham Yaolei Jiao Guilong Ma Piwu Wang College of Agronomy Jilin Agricultural UniversityChangchun1300118China College of Plant Protection Agricultural UniversityChangchun1300118China

High yield,high quality,stable yield,adaptability to growth period,and modern mechanization are the basic requirements for crops in the 21st *** oleic acid is a natural unsaturated fatty acid with strong antioxidant properties and *** as a safe fatty acid,it has the ability to successfully prevent cardiovascular and cerebrovascular *** the fatty acid composition of soybean seeds,can not only speed up the breeding process of high-quality high-oil and high-oleic soybeans,but also have important significance in human health,and provide the possibility for the development of soybean oil as a new energy ***,the aim of this study was to analyze the high oleic acid elated gene GmSAM22 in *** this research the soybean oleic acid-related gene GmSAM22 was screened out by Genome-wide association analysis,a 662 bp fragment was acquired by specific PCR amplification,and the pMD18T cloning vector was linked by the use of a seamless cloning *** analysis of the signal peptide prediction,subcellular localization,protein hydrophobicity,transmembrane region analysis,a phosphorylation site,protein secondary and tertiary structure and protein interaction analysis of the protein encoded by the SAM22 gene was carried *** plasmid of the gene editing vector is *** overexpression vector was transformed from pCAMBIA3301 as the base vector,and overexpression vector were *** plants were obtained by genetic transformation by the pollen tube channel *** quantitative PCR was performed on the T2 generation plants to detect the relative expression levels in different *** Blot was used to detect the presence of hybridization *** genes BAR,35S,and NOS in plants were identified by conventional PCR.10 seeds with high and low oleic acid content were chosen for quantitative PCR identification,and finally,the concentration and morphology of soybean fatty acids were

关键词： Soybean SAM22 gene oleic acid cloning CRISPR/Cas9 expression vector

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Laser-assisted water jet machining of high quality micro-trap structures on stainless steel surfaces

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中国光学（中英文） 2024年第6期17卷 1476-1488页

作者： LIU Li YAO Peng CHU Dong-kai XU Xiang-yue qu shuo-shuo HUANG Chuan-zhen Center for Advanced Jet Engineering Technologies(CaJET) School of Mechanical EngineeringShandong UniversityJinan 250061China Key Laboratory of High Efficiency and Clean Mechanical Manufacture Ministry of EducationShandong UniversityJinan 250061China School of Mechanical Engineering Yanshan UniversityQinhuangdao 066004China

Secondary electron emission(SEE)has emerged as a critical issue in next-generation *** SEE on metal surfaces is crucial for enhancing the stability and emittance of particle accelerators while extending their *** paper explores the application of laser-assisted water jet technology in constructing high-quality micro-trap structures on 316L stainless steel,a key material in accelerator *** study systematically analyzes the impact of various parameters such as laser repetition frequency,pulse duration,average power,water jet pressure,repeat times,nozzle offset,focal position,offset distance between grooves,and processing speed on the surface morphology of stainless *** findings reveal that micro-groove depth increases with higher laser power but decreases with increasing water jet pressure and processing ***,repeat times have minimal effect on *** the other hand,micro-groove width increases with higher laser power and repeat times but decreases with processing *** optimizing these parameters,the researchers achieved high-quality pound sign-shaped trap structure with consistent *** tested the secondary electron emission coefficient of the"well"*** coefficient is reduced by 0.5 at most compared to before processing,effectively suppressing secondary electron *** results offer indispensable insights for the fabrication of micro-trap structures on material ***-assisted water jet technology demonstrates considerable potential in mitigating SEE on metal surfaces.

关键词： laser-assisted water jet 316L stainless steel micro-trap structures "well"structure surface morphology secondary electron emission(SEE) groove depth groove width

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Bioinspired superwetting surfaces for fog harvesting fabricated by picosecond laser direct ablation

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Journal of Central South University 2022年第10期29卷 3368-3375页

作者： LI Wei-zhen CHU Dong-kai qu shuo-shuo YIN Kai HU Shuang-shuang YAO Peng Center for Advanced Jet Engineering Technologies(CaJET) School of Mechanical EngineeringShandong UniversityJinan 250061China Key Laboratory of High Efficiency and Clean Mechanical Manufacture(Shandong University) Ministry of EducationJinan 250061China Hunan Key Laboratory of Nanophotonics and Devices School of Physics and ElectronicsCentral South UniversityChangsha 410083China Department of Economics and Management Technician College of Liaocheng CityLiaocheng 252000China

Fog harvesting has been considered as a promising method for solving water crisis in underdeveloped *** mimic and optimize the alleged natural fog harvesting ability of the stenocara beetle,hybrid superhydrophobic(hydrophobic,superhydrophilic)/hydrophilic patterns are processed on stainless steel via picosecond laser direct ***,after laser processing,the surfaces of stainless steel change from hydrophilic to ***,after chemical and heat treatment,the superhydrophilic surfaces become superhydrophobic with ultra-low adhesion,and superhydrophobic(hydrophobic)with ultra-high adhesion,*** work systematically examines the fog harvesting ability of picosecond laser treated surfaces(LTS),pristine surfaces(PS),laser and chemical treated surfaces(LCTS),laser and heat-treated surfaces(LHTS).Compared with the PS,the as-prepared surfaces enhanced the fog harvesting efficiency by 50%.This work provides a fast and simple method to fog collectors,which offer a great opportunity to develop water harvesters for real world applications.

关键词： fog harvesting superhydrophobic superhydrophilic picosecond laser stenocara beetle

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单向碳纤维增强陶瓷基复合材料磨削表面质量研究

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东北大学学报（自然科学版） 2019年第9期40卷 1310-1315页

作者：屈硕硕巩亚东杨玉莹蔡明东北大学机械工程与自动化学院

为考察单向碳纤维增强陶瓷基复合材料(C f/SiC)的磨削表面质量,使用树脂结合剂金刚石砂轮完成正交试验研究.通过极差分析获得砂轮线速度 v s、磨削深度 a p和进给速度 v w对表面质量影响的主次顺序.正交试验结果表明:磨削深度对磨削表... 详细信息

为考察单向碳纤维增强陶瓷基复合材料(C f/SiC)的磨削表面质量,使用树脂结合剂金刚石砂轮完成正交试验研究.通过极差分析获得砂轮线速度 v s、磨削深度 a p和进给速度 v w对表面质量影响的主次顺序.正交试验结果表明:磨削深度对磨削表面粗糙度影响最大;随着磨削深度 a p的增大,表面粗糙度显著增大;随着砂轮线速度 v s的增大,表面粗糙度不断减小;随着进给速度 v w的增大,表面粗糙度增大.最终根据试验结果及表面微观形貌对单向碳纤维增强陶瓷基复合材料的磨削机理进行深入的分析,对单向C f/SiC磨削加工理论的机理揭示具有指导意义.

关键词：单向碳纤维增强陶瓷基复合材料(Cf/SiC) 磨削表面质量正交试验磨削机理

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镍基单晶高温合金磨削表面质量及亚表面微观组织试验

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东北大学学报（自然科学版） 2019年第3期40卷 386-391页

作者：蔡明巩亚东屈硕硕杨玉莹东北大学机械工程与自动化学院辽宁沈阳110819

采用正交试验的方法,研究了镍基单晶高温合金DD5表面质量影响因素和亚表面微观组织.进行DD5平面槽磨削正交试验,得到砂轮线速度、磨削深度和进给速度对表面质量的影响规律,优选出最优工艺参数组合,并对磨削亚表面微观组织和磨屑形貌进... 详细信息

采用正交试验的方法,研究了镍基单晶高温合金DD5表面质量影响因素和亚表面微观组织.进行DD5平面槽磨削正交试验,得到砂轮线速度、磨削深度和进给速度对表面质量的影响规律,优选出最优工艺参数组合,并对磨削亚表面微观组织和磨屑形貌进行观察.结果表明:砂轮线速度对磨削表面粗糙度Ra影响最大;随着砂轮线速度的增大,表面粗糙度Ra不断减小;随着磨削深度和进给速度的增大,表面粗糙度Ra不断增大.选出的镍基单晶高温合金DD5平面磨削试验参数范围内的最优工艺参数组合:砂轮线速度为30 m/s,磨削深度为20μm,进给速度为0. 2 m/min.磨削亚表面出现了塑性变形层和加工硬化层.磨屑主要呈现出一节一节的锯齿状特征.

关键词：镍基单晶高温合金DD5 磨削表面质量正交试验亚表面磨屑

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增/减材复合加工316L宏观形貌和残余应力试验

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东北大学学报（自然科学版） 2020年第3期41卷 380-386页

作者：杨玉莹巩亚东屈硕硕蔡明东北大学机械工程与自动化学院辽宁沈阳110819

为了研究激光功率P、送粉速率f和扫描速度vf对熔覆层宏观质量的影响,采用正交试验进行激光熔化沉积的单层单道成形试验研究.以熔覆层的形状系数ξ为评价指标,通过极差分析获得P,f和vf对几何形貌影响的主次顺序.结果表明:在一定工艺参数... 详细信息

为了研究激光功率P、送粉速率f和扫描速度vf对熔覆层宏观质量的影响,采用正交试验进行激光熔化沉积的单层单道成形试验研究.以熔覆层的形状系数ξ为评价指标,通过极差分析获得P,f和vf对几何形貌影响的主次顺序.结果表明:在一定工艺参数范围内,f对熔覆层ξ的影响最大;最佳工艺参数组合是P,f和vf分别为1 k W,0.7 g/min和600 mm/min.利用X射线衍射法对增/减材复合制造316L不锈钢薄壁圆环表面残余应力的分布进行了试验研究.结果表明:试样表面的顶部和底部是拉应力,中间部分是压应力;铣削加工可以消除一部分残余应力.

关键词：激光熔化沉积增/减材复合加工 316L不锈钢正交试验宏观形貌残余应力

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2.5D C_f/SiC复合材料磨削工艺试验研究

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东北大学学报（自然科学版） 2020年第2期41卷 252-257页

作者：屈硕硕巩亚东杨玉莹舍跃斌东北大学机械工程与自动化学院

为研究2.5D Cf/SiC复合材料的磨削工艺性能,设计并完成了单因素试验.结果表明:磨削深度a p、砂轮线速度v s及进给速度v w均对2.5D Cf/SiC的磨削性能评价参数(表面形貌、表面粗糙度及磨削力)产生影响,其中磨削深度对评价参数的影响最大,... 详细信息

为研究2.5D Cf/SiC复合材料的磨削工艺性能,设计并完成了单因素试验.结果表明:磨削深度a p、砂轮线速度v s及进给速度v w均对2.5D Cf/SiC的磨削性能评价参数(表面形貌、表面粗糙度及磨削力)产生影响,其中磨削深度对评价参数的影响最大,进给速度影响最小;经向纤维及纬向纤维的支撑效果具有很大差异,导致不同的磨削加工缺陷形式;2.5D Cf/SiC的主要缺陷形式包括界面脱粘、裂纹、纤维露头及拔除;不同加工参数及纤维方向会导致磨屑出现一定的差异.根据试验结果对2.5D Cf/SiC的磨削加工机理进行了考察分析,为2.5D Cf/SiC的磨削加工理论的研究提供了重要的技术支撑.

关键词： 2.5D Cf/SiC 表面形貌表面粗糙度磨削力磨削机理

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