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RNA barcode segments for SARS-CoV-2 identification from HCoVs and SARSr-CoV-2 lineages

Virologica Sinica 2024年第1期39卷 156-168页

作者： Changqiao You Shuai Jiang Yunyun Ding shunxing ye Xiaoxiao Zou Hongming Zhang Zeqi Li Fenglin Chen Yongliang Li Xingyi Ge Xinhong Guo College of Biology Hunan UniversityChangsha410082China College of Bioscience and Biotechnology Hunan Agricultural UniversityChangsha410128China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the pathogen responsible for coronavirus disease 2019(COVID-19),continues to evolve,giving rise to more variants and global *** research has demonstrated that barcode segments can effectively and cost-efficiently identify specific species within closely related *** this study,we designed and tested RNA barcode segments based on genetic evolutionary relationships to facilitate the efficient and accurate identification of SARS-CoV-2 from extensive virus samples,including human coronaviruses(HCoVs)and SARSr-CoV-2 *** sequences sourced from NCBI and GISAID were meticulously selected and curated to construct training sets,encompassing 1733 complete genome sequences of HCoVs and SARSr-CoV-2 *** genetic-level species testing,we validated the accuracy and reliability of the barcode segments for identifying ***,75 main and subordinate species-specific barcode segments for SARS-CoV-2,located in ORF1ab,S,E,ORF7a,and N coding sequences,were intercepted and screened based on single-nucleotide polymorphism sites and weighted ***-testing,these segments exhibited high recall rates(nearly 100%),specificity(almost 30%at the nucleotide level),and precision(100%)performance on *** were eventually visualized using one and two-dimensional combined barcodes and deposited in an online database(http://***/).The successful integration of barcoding technology in SARS-CoV-2 identification provides valuable insights for future studies involving complete genome sequence polymorphism ***,this cost-effective and efficient identification approach also provides valuable reference for future research endeavors related to virus surveillance.

关键词： RNA barcode segments SARS-CoV-2 variants and related lineages HCoVs Genetic tests Complete genome sequences

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重力场和地幔流应力场的关系

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西安地质学院学报 1989年第4期11卷 84-89页

作者：叶顺祥西安地质学院物探系

本文从地幔是Lamina牛顿可压缩粘滞性流体假设出发,根据流体力学推导出重力场和地幔流应力场的基本关系为: 此关系式为人们根据重力场认识地幔流对岩石圈的作用提供一新的途径。

关键词：重力场地幔流应力场岩石圈

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Nitrogen-doped carbon@TiO_(2) double-shelled hollow spheres as an electrochemical sensor for simultaneous determination of dopamine and paracetamol in human serum and saliva

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Journal of Pharmaceutical Analysis 2022年第3期12卷 436-445页

作者： Hui Yang Gongxun Cao Yongjun Huang ye Lin Fengying Zheng Luxiu Lin Fengjiao Liu shunxing Li College of Chemistry Chemical Engineering and EnvironmentMinnan Normal UniversityZhangzhouFujian363000China Fujian Provincial Key Laboratory of Pollution Monitoring and Control Minnan Normal UniversityZhangzhouFujian363000China Fujian Key Laboratory of Separation and Analysis Science and Technology ZhangzhouFujian363000China

As the most commonly used antipyretic and analgesic drug,paracetamol(PA)coexists with neurotransmitter dopamine(DA)in real biological *** simultaneous determination is extremely important for human health,but they also interfere with each *** order to improve the conductivity,adsorption affinity,sensitivity,and selectivity of TiO_(2)-based electrochemical sensor,N-doped carbon@-TiO_(2) double-shelled hollow sphere(HeC/N@TiO_(2))is designed and synthesized by simple alcoholic and hydrothermal method,using polystyrene sphere(PS)as a ***,TiO_(2) hollow spheres(H eTiO_(2))or N-doped carbon hollow spheres(HeC/N)are also prepared by the same ***/N@TiO_(2) has good conductivity,charge separation,and the highly enhanced and stable current responses for the detection of PA and *** detection limit and linear range are 50.0 nmol/L and 0.3-50 mmol/L for PA,40.0 nmol/L and 0.3e50 mmol/L for DA,respectively,which are better than those of carbon-based ***,this electrochemical sensor,with high selectivity,strong anti-interference,high reliability,and long time durability,can be used for the simultaneous detection of PA and DA in human blood serum and *** high electrochemical performance of HeC/N@TiO_(2) is attributed to the multifunctional combination of different layers,because of good conductivity,absorption and electrons transfer ability from in-situ N-doped carbon and electrocatalytic activity from TiO_(2).

关键词： Electrochemical sensor Simultaneous determination Paracetamol Dopamine

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RESEARCH AND APPLICATION ON THE VCN OF n-BITS

RESEARCH AND APPLICATION ON THE VCN OF n-BITS

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中南模糊数学和系统分会第三届年会

作者： ye Qiusun Liu shunxing (Computing Center of Jian Yang Commercial School,Jian Yang FuJian 354200)

This paper bases itself upon the analysing and researching of numbers’ canying,and gives out a new and broad concept of numbers,namely the VCN of *** it points out that,at the moment,in international Maths and Computer Science,the basic reasons of puzzled realm in which numbers have been deeply studied and developed at a standstill are all researches on numbers only confined to a same varying rule of the *** the meantime,it also points out that,the application of VCN for n-bits is wider than that of FCN for n-bits,and it gives out some applied examples.

关键词： VCN-variable-carrying numbers FCN-fixed-canTing numbers

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