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Gga-miRNA-181-5p family facilitates chicken myogenesis via targeting TGFBR1 to block TGF-βsignaling

Journal of Integrative Agriculture 2024年第8期23卷 2764-2777页

作者： Xiaoxu Shen Yongtong Tian Wentao He Can He shunshun han Yao han Lu Xia Bo Tan Menggen Ma Houyang Kang Jie Yu Qing Zhu Huadong Yin Key Laboratory of Livestock and Poultry Multi-omics Ministry of Agriculture and Rural AffairsCollege of Animal Science and TechnologySichuan Agricultural UniversityChengdu 611130China Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province Sichuan Agricultural UniversityChengdu 611130China College of Forestry Sichuan Agricultural UniversityChengdu 611130China College of Resources Sichuan Agricultural UniversityChengdu 611130China Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130China Key Laboratory for Animal Disease Resistance Nutrition of China Institute of Animal NutritionMinistry of Education/Sichuan Agricultural UniversityChengdu 611130China

MicroRNAs(miRNAs)have been demonstrated to control chicken skeletal muscle growth,however,the potential function of the miR-181-5p family in chicken myogenesis remains largely ***,our study identified the two chicken(Gallus gallus;Gga)miR-181-5p family members widely expressed in various tissues,specifically miR-181a-5p and ***,the breast muscles of fast-growing broilers expressed higher levels of miR-181a-5p and miR-181b-5p than those of slow-growing ***,miR-181a-5p and miR-181b-5p both promote the expression level of myogenic factors including myogenin(MyoG),myogenic differentiation 1(MyoD1),and myosin heavy chain(MyHC),meanwhile accelerating the myotube formation of skeletal muscle satellite cells(SMSCs).Mechanistically,miR-181a-5p and miR-181b-5p directly bind to the 3′untranslated region(UTR)of the transforming growth factor beta receptor 1(TGFBR1)mRNA,further reducing the expression of ***1 is a key Transforming growth factor beta(TGF-β)signaling transduction receptor and had a negative function in muscle cell ***,knockdown of TGFBR1 facilitated the expression of chicken myogenic factors,boosted myotube formation,and decreased the SMAD family member 2/3(SMAD2/3)phosphorylation in chicken ***2/3 are downstream of TGF-βsignaling,and miR-181a-5p and miR-181b-5p could reduce the expression of TGFBR1 to further diminish the SMAD2/3 *** findings revealed that the miR-181-5p family targets TGFBR1 to break the TGF-βsignaling transduction,which resulted in promoting chicken skeletal muscle development.

关键词： miRNA-181-5p family SMSCs differentiation TGFBR1 TGF-βsignaling

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Phenotypic Image Recognition of Asparagus Stem Blight Based on Improved YOLOv8

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Computers, Materials & Continua 2024年第9期80卷 4017-4029页

作者： shunshun Ji Jiajun Sun Chao Zhang College of Information Science and Engineering Shandong Agricultural UniversityTai’an271000China

Asparagus stem blight,also known as“asparagus cancer”,is a serious plant disease with a regional *** widespread occurrence of the disease has had a negative impact on the yield and quality of asparagus and has become one of the main problems threatening asparagus *** improve the ability to accurately identify and localize phenotypic lesions of stem blight in asparagus and to enhance the accuracy of the test,a YOLOv8-CBAM detection algorithm for asparagus stem blight based on YOLOv8 was *** algorithm aims to achieve rapid detection of phenotypic images of asparagus stem blight and to provide effective assistance in the control of asparagus stem *** enhance the model’s capacity to capture subtle lesion features,the Convolutional Block AttentionModule(CBAM)is added after C2f in the ***,the original CIoU loss function in YOLOv8 was replaced with the Focal-EIoU loss function,ensuring that the updated loss function emphasizes higher-quality bounding *** YOLOv8-CBAM algorithm can effectively detect asparagus stem blight phenotypic images with a mean average precision(mAP)of 95.51%,which is 0.22%,14.99%,1.77%,and 5.71%higher than the YOLOv5,YOLOv7,YOLOv8,and Mask R-CNN models,*** greatly enhances the efficiency of asparagus growers in identifying asparagus stem blight,aids in improving the prevention and control of asparagus stem blight,and is crucial for the application of computer vision in agriculture.

关键词： YOLOv8 asparagus stem blight image recognition pest

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Excellent energy storage properties in non-stoichiometric Bi_(0.5)Na_(0.5)TiO_(3)-based relaxor ferroelectric ceramics

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Chinese Chemical Letters 2024年第7期35卷 470-472页

作者： shunshun Jiang Ji Zhang Jing Wang Shan-Tao Zhang School of Materials Science and Engineering Nanjing University of Science&TechnologyNanjing 210094China State Key Laboratory of Mechanics and Control of Mechanical Structures College of Aerospace EngineeringNanjing University of Aeronautics and AstronauticsNanjing 210016China National Laboratory of Solid State Microstructures Department of Materials Science and EngineeringCollege of Engineering and Applied Science&Jiangsu Key Laboratory of Artificial Functional Materials&Collaborative Innovation Center of Advanced MicrostructuresNanjing UniversityNanjing 210093China

The rapid development of high-power and pulsed-power techniques inspires extensive investigates on high-performance ceramic-based ***,the low recoverable energy density(Wrec)hampers their wider ***,the non-stoichiometric Bi_(0.5)Na_(0.5)TiO_(3)-based ceramics were designed and *** proper introduction of oxygen vacancies facilitated activating defect dipole,giving rise to reduced remanent ***,the optimal composition exhibited an exceptional high Wrec of 8.3 J/cm^(3),a high efficiency of 85%,and excellent anti-fatigue and thermal *** work provides an efficient approach to explore ceramic capacitors with high capacitive energy storage performances.

关键词： Non-stoichiometric Bi_(0.5)Na_(0.5)TiO_(3) Ceramic capacitor Energy density Thermal stability

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Highly robust zinc metal anode directed by organic–inorganic synergistic interfaces for wearable aqueous zinc battery

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SmartMat 2024年第1期5卷 135-146页

作者： Xixi Zhang Chuanlin Li Guangmeng Qu Chenggang Wang shunshun Zhao Tongkai Wang Na Li Xiaojuan Li Xijin Xu School of Physics and Technology University of JinanJinanChina School of Chemistry and Chemical Engineering Shandong UniversityJinanChina State Key Laboratory of Chemical Resource Engineering Beijing Key Laboratory of Electrochemical Process and Technology of MaterialsBeijing University of Chemical TechnologyBeijingChina

Flexible aqueous zinc batteries(FAZBs)with high safety and environmental friendliness are promising smart power sources for smart wearable ***,the bare zinc anode usually suffers from damnable dendrite growth and rampant side reaction on the surface,greatly impeding practical applications in ***,a composite polymer interface layer is artificially self‐assembled on the surface of the zinc anode by graft‐modified fluorinated monomer(polyacrylic acid‐2‐(Trifluoromethyl)propenoic acid,PAA‐TFPA),on which an organic–inorganic hybrid(PAA‐Zn/ZnF2)solid electrolyte interface(SEI)with excellent ionic conductivity is formed by interacting with Zn2+.Both the pouch cell and fiber zinc anode exhibit excellent plating/stripping reversibility after protecting by this organic–inorganic SEI,which can be stably cycled more than 3000 h in symmetric Zn||Zn cells or 550 h in fiber Zn||Zn ***,this interface layer preserves zinc anode with excellent mechanical durability under various mechanical deformation(stably working for another 1200 h after bending 100 h).The corresponding PAA‐Zn/ZnF2@Zn||MnO2 full cell displays an ultra‐long life span(79%capacity retention after 3000 cycles)and mechanical robustness(85%of the initial capacity for another 3000 cycles after bending 100 times).More importantly,the as‐assembled cells can easily power smart wearable devices to monitor the user's health condition.

关键词： hybrid SEI mechanical durability wearable zinc battery zinc anode

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中国产业部门碳排放增长的结构性动因--基于SDA与LMDI耦合分解法

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资源科学 2024年第5期46卷 881-894页

作者：杨顺顺湖南省社会科学院(湖南省人民政府发展研究中心)经济研究所长沙410003

[目的]技术进步和需求结构的优化调整使产业部门低碳转型具备结构性动因,通过分解影响碳排放增长的各类因素,从而分析这一变化规律将为宏观减排路线谋划提供理论支撑。[方法]本文耦合结构分解分析(SDA)和对数平均权重Divisia指数法(LMDI... 详细信息

[目的]技术进步和需求结构的优化调整使产业部门低碳转型具备结构性动因,通过分解影响碳排放增长的各类因素,从而分析这一变化规律将为宏观减排路线谋划提供理论支撑。[方法]本文耦合结构分解分析(SDA)和对数平均权重Divisia指数法(LMDI)两类经典分解法,研制2005-2020年可比价投入产出表,对产业部门整体和细分行业部门碳排放增长的驱动因素进行了识别和分析。[结果](1)2005-2020年,产业部门碳排放总量由48.67亿t增长至106.77亿t,但5年平均增速已下降至2%以下;需求侧视角下,高碳部门向产业链下游延伸。(2)涉及技术进步的因素中,能源强度和生产结构效应的负贡献强于能源结构效应;涉及需求结构的因素中,投资扩张效应的正贡献始终强于消费拉动和出口变动效应;“十三五”以来,消费拉动效应的增碳和生产结构效应的减碳作用提升显著,部门间技术进步开始主导碳减排效应。(3)“十三五”时期,资源加工工业、电力热力燃气和水的生产和供应业、服务业整体处碳增长阶段,前者和后两者分别主要受投资扩张和消费拉动效应影响;采掘业、轻纺工业、机械及电子工业均由历史上的碳增长转向碳削减阶段,前者和后两者分别主要受进口替代和生产结构效应影响。[结论]2015年后,产业部门碳排放各类驱动因素的效力变化明显,促进最终需求结构和能源结构调整、产业链协同减排以及涉碳市场优化是未来产业部门碳减排的关注重点。

关键词：产业部门碳排放结构分解分析 LMDI模型驱动因素中国

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YAP Inactivation by Soft Mechanotransduction Relieves MAFG for Tumor Cell Dedifferentiation

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Research 2024年第2期6卷 401-415页

作者： Jiadi Lv Xiaohan Liu Yabo Zhou Feiran Cheng Haoran Chen shunshun Li Dianheng Wang Li Zhou Zhenfeng Wang Nannan Zhou Jie Chen Bo Huang Department of Immunology&State Key Laboratory of Common Mechanism Research for Major Diseases Institute of Basic Medical SciencesChinese Academy of Medical Sciences&Peking Union Medical College(PUMC)Beijing100005China Department of Histology and Embryology Basic Medical CllegeChina Medical UniversityShenyangLiaoning 11122China Department of Immunology Basic Medical Cllege China Medical UniversityShenyangLiaoning 110122China Department of Biochemistry&Molecular Biology Tongji Medical CollegeHuazhong University of Science&TechnologyWuhan 430030China

Solid tumor cells live in a highly dynamic mechanical *** the extracellular-matrix-generated mechanotransduction regulates tumor cell development and differentiation remains an ***,we show that a low mechanical force generated from the soft matrix induces dedifferentiation of moderately stiff tumor cells to soft stem-cell-like ***,integrin?was identified to transduce mechano-signaling to trigger tumor cell dedifferentiation by recruiting RhoGDI1 to inactivate RhoA and subsequently Yes-associated protein(YAP).YAP inactivation relieved the inhibition of v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog G(MAFG),allowing MAFG to transactivate the stemness genes NANOG,SOX2,and *** also restoredβ8 expression,thereby forming a closed mechanical ***,MAFG expression is correlated with worse *** findings provide mechanical insights into the regulation of tumor cell dedifferentiation,which has therapeutic implications for exploring innovative strategies to attack malignancies.

关键词： activation thereby MAF

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黄芪多糖对鸡淋巴细胞体外增殖及脾脏淋巴细胞分泌细胞因子和相关mRNA表达的影响

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四川农业大学学报 2018年第5期36卷 674-680页

作者：朱轶锋韩顺顺张克英尹华东四川农业大学动物营养所成都611130 四川农业大学动物科技学院成都611130

【目的】探讨黄芪多糖(APS)对雏鸡不同免疫组织淋巴细胞体外增殖和脾脏淋巴因子分泌及相关基因mRNA表达的影响。【方法】将浓度为20、40、80、160以及320μg/mL的APS分别加入用刀豆蛋白(ConA)刺激的体外培养雏鸡外周血、胸腺、脾脏和法... 详细信息

【目的】探讨黄芪多糖(APS)对雏鸡不同免疫组织淋巴细胞体外增殖和脾脏淋巴因子分泌及相关基因mRNA表达的影响。【方法】将浓度为20、40、80、160以及320μg/mL的APS分别加入用刀豆蛋白(ConA)刺激的体外培养雏鸡外周血、胸腺、脾脏和法氏囊淋巴细胞中,采用MTT比色法测定淋巴细胞增殖情况,ELISA检测脾脏淋巴细胞中Th1型细胞因子(IL-2、TNF-β、IFN-γ)和Th2型细胞因子(IL-4、IL-6)的含量,RT-qPCR检测细胞因子相关基因以及核转录因子TBX21和Gata-3基因mRNA表达量。【结果】浓度为80～320μg/mL的APS可显著刺激外周血、胸腺、脾脏和法氏囊淋巴细胞增殖,明显促进细胞因子IL-2、TNF-β、IFN-γ、IL-4、IL-6的分泌以及相关基因mRNA的表达,并可显著提高TBX21和Gata-3的mRNA表达量,其中APS为160μg/mL效果最佳。【结论】APS可通过促进淋巴细胞Th1和Th2细胞因子分泌,提高淋巴细胞增殖,从而参与调解机体细胞免疫。

关键词：黄芪多糖淋巴细胞免疫组织细胞增殖淋巴因子雏鸡

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Rocking-chair ammonium ion battery with high rate and long-cycle life

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Chinese Chemical Letters 2024年第1期35卷 563-567页

作者： Tongkai Wang Xiaojuan Li shunshun Zhao Hongxia Bu Chuanlin Li Na Li Xixi Zhang Xijin Xu School of Physics and Technology University of JinanJi'nan 250022China State Key Laboratory of Chemical Resource Engineering Beijing Key Laboratory of Electrochemical Process and Technology of MaterialsBeijing University of Chemical TechnologyBeijing 100029China College of Physics and Electronic Engineering Qilu Normal UniversityJi'nan 250200China

Aqueous rechargeable ammonium-ion batteries(AIBs)have drew considerable attention because of their capacity for high rates,low cost,and high ***,developing desired electrodes requiring stable structure in the aqueous fast ammoniation/de-ammoniation becomes ***,an ammonium ion full battery using Cu_(3)[Fe(CN)_(6)]_(2)(CuHCF)acting to be a cathode and barium vanadate(BVO)acting to be an anode is *** excellent electrochemical behavior of Prussian blue analogs and the perfectly matched lattice structure of NH_(4)^(+)is *** the open structure of vanadium compounds satisfies the fast ammoniation/de-ammoniation of NH4+is also *** a result of these synergistic effects,the BVO//CuHCF full cell retains 80.5 percent of its capacity following 1000 *** achievements provide new ideas for developing low-cost and long-life AIBs.

关键词： Copper hexacyanoferrate Vanadium-based compounds Aqueous ammonium ion batteries Long-term cyclability Ammonium ion storage mechanism

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谁在“一带一路”教育行动中更积极——对20个“一带一路”高校联盟成员国家的合作网络分析

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湖南师范大学教育科学学报 2024年第4期23卷 98-106页

作者：胡顺顺姚威安徽大学马克思主义学院安徽合肥230601 浙江大学中国科教战略研究院浙江杭州310058

基于20个“一带一路”高校联盟的成员国家数据,使用社会网络分析方法研究了“一带一路”高校联盟的成员国家合作网络结构特征与演变规律。研究发现,联盟网络整体紧密性较强,除中国占据联盟网络核心位置外,马来西亚、巴基斯坦和俄罗斯等... 详细信息

基于20个“一带一路”高校联盟的成员国家数据,使用社会网络分析方法研究了“一带一路”高校联盟的成员国家合作网络结构特征与演变规律。研究发现,联盟网络整体紧密性较强,除中国占据联盟网络核心位置外,马来西亚、巴基斯坦和俄罗斯等国家在共建“一带一路”教育行动中更积极,在网络中处于优势位置。但是,中介中心度结果显示“一带一路”高校联盟的成员国家间依赖程度不高,信息和权力相对分散,且演变分析显示联盟网络仍属于成长型网络。为此,建议进一步激发“一带一路”合作国家共建教育行动的积极性,提升各联盟内部及不同联盟之间的合作紧密性,加强对联盟的宏观指导与精准治理,扩大数字技术在联盟合作中的应用和推广。

关键词： “一带一路”教育行动高校联盟社会网络

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火车轮对再制造用ER6车轮钢粉末的制备及其性能表征

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安徽工程大学学报 2021年第5期36卷 9-14,68页

作者：顾文键朱协彬程敬卿汪超韩顺顺安徽工程大学材料科学与工程学院安徽芜湖241000 安徽鼎恒再制造产业技术研究院安徽芜湖241000

采用氮气雾化制备火车轮对再制造用ER6车轮钢粉末,并通过激光粒度分析仪、扫描电子显微镜和霍尔流速计等表征手段研究了不同粒径的ER6车轮钢粉末的粒度分布、表面形貌及内部微观组织、流动性和松装密度。结果表明:氮气雾化制备的ER6车... 详细信息

采用氮气雾化制备火车轮对再制造用ER6车轮钢粉末,并通过激光粒度分析仪、扫描电子显微镜和霍尔流速计等表征手段研究了不同粒径的ER6车轮钢粉末的粒度分布、表面形貌及内部微观组织、流动性和松装密度。结果表明:氮气雾化制备的ER6车轮钢粉末粒径呈正态分布,主要集中在17~158μm之间,中粒径d_(50)≈55μm,粉末球形度较高;随着粉末粒径的减小,粉末表面越光滑,卫星颗粒减少;金属粉末表面和内部组织主要由枝状晶和胞状晶组成,但粒径较小的粉末因冷却速率较高,胞状晶晶粒明显细化且枝状晶数量减少;所制备的ER6车轮钢粉末流动性和松装密度分别在19.22~24.47 s/50 g和4.11~4.43 g/cm^(3)范围内,呈现出良好的稳定性。

关键词：氮气雾化 ER6车轮钢粉末微观组织流动性松装密度

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