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Involvement of A5/A7 noradrenergic neurons and B2 serotonergic neurons in nociceptive processing:a fiber photometry study

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Neural Regeneration Research 2022年第4期17卷 881-886页

作者： shunpei moriya Akira Yamashita Daiki Masukawa Junichi Sakaguchi Yoko Ikoma Yoshimune Sameshima Yuki Kambe Akihiro Yamanaka Tomoyuki Kuwaki Department of Physiology Kagoshima University Graduate School of Medical and Dental ScienceKagoshimaJapan Department of Molecular Pharmacologyand Neurobiology Yokohama City University Graduate School of MedicineYokohamaJapan Department of Pharmacology Kagoshima University Graduate School of Medical and Dental ScienceKagoshimaJapan Research Institute of Environmental Medicine Nagoya UniversityNagoyaJapan

In the central nervous system,the A6 noradrenaline(NA)and the B3 serotonin(5-HT)cell groups are well-recognized players in the descending antinociceptive system,while other NA/5-HT cell groups are not well characterized.A5/A7 NA and B25-HT cells project to the spinal horn and form descending *** recorded G-Ca MP6 green fluorescence signal intensities in the A5/A7 NA and the B25-HT cell groups of awake mice in response to acute tail pinch stimuli,acute heat stimuli,and in the context of a non-noxious control test,using fiber photometry with a calcium imaging *** first introduced G-Ca MP6 in the A5/A7 NA or B25-HT neuronal soma,using transgenic mice carrying the tetracycline-controlled transactivator transgene under the control of either a dopamineβ-hydroxylase or a tryptophan hydroxylase-2 promoters and by the site-specific injection of adeno-associated virus(AAV-Tet O(3 G)-G-Ca MP6).After confirming the specific expression patterns of G-Ca MP6,we recorded G-Ca MP6 green fluorescence signals in these sites in awake mice in response to acute nociceptive stimuli.G-Ca MP6 fluorescence intensity in the A5,A7,and B2 cell groups was rapidly increased in response to acute nociceptive stimuli and soon after,it returned to baseline fluorescence *** was not observed in the non-noxious control *** results indicate that acute nociceptive stimuli rapidly increase the activities of A5/A7 NA or B25-HT neurons but the non-noxious stimuli do *** present study suggests that A5/A7 NA or B25-HT neurons play important roles in nociceptive processing in the central nervous *** suggest that A5/A7/B2 neurons may be new therapeutic *** performed procedures were approved by the Institutional Animal Use Committee of Kagoshima University(MD17105)on February 22,2018.

关键词： A5 NA neurons A7 NA neurons B25-HT neurons DBH-tTA mice fiber photometry G-CaMP6 mCherry monoaminergic signaling nociception TPH-t TA mice

麦根腐平脐蠕孢cDNA文库的构建及BsTup1互作蛋白筛选

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河南农业大学学报 2024年第2期58卷 218-227页

作者：李长水耿月华姚萌赵炳森谢顺培徐超马庆周张猛河南农业大学植物保护学院菌物研究所河南郑州450046

【目的】深入探究麦根腐平脐蠕孢(Bipolaris sorokiniana)生长发育及致病力的分子作用机制,并鉴定BsTup1的互作蛋白。【方法】利用麦根腐平脐蠕孢(***)孢子和不同时期的菌丝体为材料,构建酵母双杂交cDNA文库,以BsTup1基因为诱饵来筛选... 详细信息

【目的】深入探究麦根腐平脐蠕孢(Bipolaris sorokiniana)生长发育及致病力的分子作用机制,并鉴定BsTup1的互作蛋白。【方法】利用麦根腐平脐蠕孢(***)孢子和不同时期的菌丝体为材料,构建酵母双杂交cDNA文库,以BsTup1基因为诱饵来筛选酵母双杂交文库,确定与BsTup1相互作用的蛋白。【结果】1)利用SMART(switching mechanism at 5′end of the RNA transcript)技术首次成功构建了麦根腐平脐蠕孢(***-ana)分生孢子和菌丝体的混合cDNA文库。文库鉴定结果表明,构建的cDNA文库库容为4.8×10^(7) cfu·mL^(-1),文库插入片段重组率达100%且平均大小为1000 bp。2)构建了pGBKT7-BsTup1诱饵载体,无自激活活性。3)使用诱饵蛋白载体pGBKT7-BsTup1对麦根腐平脐蠕孢(***)酵母双杂交cDNA文库进行筛选,经测序、序列比对和酵母回转验证,获得38个与BsTup1相互作用的候选蛋白。【结论】成功构建了麦根腐平脐蠕孢(***)的cDNA文库,并鉴定出38个与BsTup1相互作用的候选蛋白。

关键词：麦根腐平脐蠕孢 SMART技术 cDNA文库酵母双杂 BsTup1

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大直径桥桩近接运营地铁施工扰动的分区控制

土工基础 2024年第2期38卷 199-203页

作者：黄顺佩王良俊朱尚明翟浪宝中国水利水电第七工程局有限公司成都611730 中电建铁路建设投资集团有限公司北京100070 武汉理工大学武汉430070

桥梁桩基近接既有地铁隧道是开拓城市地下空间时常遇到的问题,探讨桥桩施工对周围土层扰动规律是保证既有地铁隧道安全的重要一环。依据相应土层受扰规律提供更为安全和经济的施工工艺同样也是相关研究目的所在。依托福州某大直径超长... 详细信息

桥梁桩基近接既有地铁隧道是开拓城市地下空间时常遇到的问题,探讨桥桩施工对周围土层扰动规律是保证既有地铁隧道安全的重要一环。依据相应土层受扰规律提供更为安全和经济的施工工艺同样也是相关研究目的所在。依托福州某大直径超长桥桩近接地铁隧道施工工程,基于理论及数值分析结果,揭示了地层扰动传播随开挖深度的三阶段演化特征,并提出了一种“上部永久钢护筒微扰动贯入+下部泥浆护壁成孔组合的超长大直径桩快速施工方法”。现场实测结果显示,该方法经济、安全,隧道扰动最终变形均在安全控制值内。

关键词：桩隧工程盾构隧道土体扰动组合工艺

Development of a Hybrid Pin Joint with a Compressed Wooden Dowel and Metal Pipe

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Open Journal of Civil Engineering 2015年第1期5卷 84-96页

作者： Kiho Jung shunpei Nakamine Faculty of Education Shizuoka University Shizuoka Japan

A newly developed hybrid pin (HP), composed of a compressed wooden dowel inserted into a stainless steel pipe is suggested in this research. This configuration is expected to grant high stiffness by bending performance of the metal pipe and rich ductility through shear deformation of compressed wooden dowel without brittle split of the joint member. Experimental tests were performed in order to verify your assumptions and pursue an optimum design. Double shear test perpendicular to the grain of HP was conducted with parameter of thickness and loading direction for base member for pin’s diameter. Rotational test for mortise and tenon joint inserted with HP was performed in order to evaluate the moment resisting performance. Consequently, the hybrid pin showed satisfactory performance as shear type fastener by virtues of not only relatively high stiffness but also rich ductility originated from the properties of each component, stain less steel pipe and compressed wood.

关键词： Hybrid Pin Compressed Wooden Dowel Double Shear Moment Resisting Performance

Development of Epstein-Barr virus-associated gastric cancer:Infection,inflammation,and oncogenesis

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World Journal of Gastroenterology 2022年第44期28卷 6249-6257页

作者： Hisashi Iizasa Andy Visi Kartika Sintayehu Fekadu shunpei Okada Daichi Onomura Afifah Fatimah Azzahra Ahmad Wadi Mosammat Mahmuda Khatun Thin Myat Moe Jun Nishikawa Hironori Yoshiyama Department of Microbiology Shimane University Faculty of MedicineIzumo 693-8501ShimaneJapan Faculty of Medicine Muslim University of IndonesiaMakassar 90231Indonesia Department of Medical Microbiology and Parasitology Hawassa UniversityCollege of Medicine and Health ScienceHawassa 1560Ethiopia Faculty of Laboratory Science Yamaguchi University Graduate School of MedicineUbe 755-8505Japan

Epstein-Barr virus(EBV)-associated gastric cancer(EBVaGC)cells originate from a single-cell clone infected with ***,more than 95%of patients with gastric cancer have a history of Helicobacter pylori(***)infection,and *** is a major causative agent of gastric ***,it has long been argued that *** infection may affect the development of EBVaGC,a subtype of gastric *** gastrointestinal inflammation,a symptom of *** infection,is observed in the gastric mucosa of ***,it remains unclear whether *** infection is a cofactor for gastric carcinogenesis caused by EBV infection or whether *** and EBV infections act independently on gastric cancer *** has been reported that EBV infection assists in the oncogenesis of gastric cancer caused by *** *** contrast,several studies have reported that *** infection accelerates tumorigenesis initiated by EBV *** reviewing both clinical epidemiological and experimental data,we reorganized the role of *** and EBV infections in gastric cancer formation.

关键词： Helicobacter pylori Epstein-Barr virus Epstein-Barr virus-associated gastric cancer Coreceptor Inflammation Oncogenesis

Electrical Stimulation in Addition to Passive Exercise Has a Small Effect on Spasticity and Range of Motion in Bedridden Elderly Patients: A Pilot Randomized Crossover Study

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Health 2019年第8期11卷 1072-1086页

作者： Hideki Kataoka shunpei Nakashima Hideki Aoki Kyo Goto Junichiro Yamashita Yuichiro Honda Yasutaka Kondo Tatsuya Hirase Junya Sakamoto Minoru Okita Department of Rehabilitation Nagasaki Memorial Hospital Nagasaki Japan Department of Physical Therapy Science Nagasaki University Graduate School of Biomedical Sciences Nagasaki Japan Department of Rehabilitation Nagasaki University Hospital Nagasaki Japan Department of Rehabilitation Nagasaki Genbaku Hospital Nagasaki Japan Institute of Biomedical Sciences Nagasaki University Nagasaki Japan

Purpose: Belt electrode skeletal muscle electrical stimulation (B-SES) can stimulate large portions of muscles including deep sites without localisation of the stimulation area. The purpose of this study is to investigate both immediate treatment effects of B-SES and long-term treatment effects of B-SES with passive exercise on range of motion (ROM) and muscle tone of lower extremities in bedridden elderly patients. Methods: Outcome measures before and after B-SES treatment alone (4 Hz, 20 min, both lower extremities) were examined for the immediate effect. Outcome measures were: ROM and Modified Ashworth scale (MAS) of hip flexion and adduction;knee flexion and extension;and knee joint distance at position of flexion abduction in hip (distance of knee). A randomized crossover trial was conducted to examine the long-term effect of adding B-SES to passive exercise on ROM and MAS. Results and Discussion: The immediate effect study had 18 patients. ROM and MAS of 4 joint angles in 2 joints and distance of knee significantly improved after B-SES treatment. The long-term effect study had 11 patients. Friedman test revealed ROM and MAS of 4 joint angles in 2 joints and distance of knee significantly improved during B-SES intervention but not control intervention. B-SES in addition to passive stretch has a more statistically significant effect on contracture and spasticity in large portions of the lower extremities of bedridden elderly patients than passive stretching alone. Conclusions: We consider B-SES a useful tool to improve the ROM in lower extremities of bedridden patients.

关键词： Bedridden Patients Belt Electrode Skeletal Muscle Electrical Stimulation Lower Extremity Muscle Tone Restriction of Range of Joint Motion

Diaporthe actinidiicola:A Novel Species Causing Branch Canker or Dieback of Fruit Trees in Henan Province,China

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Diaporthe actinidiicola:A Novel Species Causing Branch Canke...

中国植物病理学会2023年学术年会

作者： CAO Jiayuan GAO Wenkai YAO Meng XIE shunpei YIN Xinming XU Chao WU Haiyan ZHANG Meng GUO Yashuang College of Plant Protection Henan Agricultural University

Pear(Pyrus),walnut(Juglans) and kiwifruit(Actinidia) are important cash crops in ***,symptoms of branch canker or dieback have been frequently observed in many orchards in Henan Province and result in high losses in fruit *** were collected for etiological isolation from three hosts during 2020 and *** this study,seven isolates were collected from branches of three hosts and identified as one novel species,based on morphological characteristics and phylogenetic analyses of the concatenated internal transcribed spacer of the ribosomal DNA(ITS),translation elongation factor 1-α(TEF),α-tubulin(TUB),histone(HIS) and calmodulin(CAL).We proposed the name Diaporthe actinidiicola and(completed Koch’s postulates to confirm *** range evaluation suggested that *** could infect all inoculated fruit trees pear,kiwifruit,walnut,apple(Malus) and peach(Prunus) and the isolates from different hosts were most virulent on the original *** study characterises a novel Diaporthe species from three different hosts in China and contributes useful data for practicable disease management.

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针推并治寰枢椎错缝19例临床观察

新中医 2001年第5期33卷 42-43页

作者：许舜沛柴铁劬广州中医药大学针灸推拿学院广东广州510405

为了提高少年儿童的寰枢椎错缝的临床疗效，对１９例患者进行了针刺风池（双）、风府、哑门、天柱（双）、后溪（双）等结合枕颌牵旋侧扳复位法治疗。结果治愈１３例，占６８．４％；有效６例，占３１．６％；总有效率１００％；认为针... 详细信息

为了提高少年儿童的寰枢椎错缝的临床疗效，对１９例患者进行了针刺风池（双）、风府、哑门、天柱（双）、后溪（双）等结合枕颌牵旋侧扳复位法治疗。结果治愈１３例，占６８．４％；有效６例，占３１．６％；总有效率１００％；认为针刺配合推拿手法治疗本病可活血化瘀、解痉止痛、整复错移．治疗效果显著；同时也强调病愈后应保持坐、卧姿的正确位置．才能巩固疗效，防止复发．

关键词：寰枢错缝针刺疗法推拿手法治疗儿童

针刀疗法对膝骨关节炎兔关节软骨细胞凋亡的影响

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辽宁中医杂志 2006年第7期33卷 894-895页

作者：何新芳明顺培俞杰广州中医药大学针灸推拿学院武警北京总队第二医院

目的:观察针刀疗法治疗膝骨关节炎兔对其细胞凋亡的影响,探讨可能的作用机制。方法:正常新西兰兔随机分为:正常组、模型组、针刀组、中药组。左后膝伸直位石膏管型固定6周建立膝骨关节炎兔模型,原位末端标记(TUNEL)检测关节软骨细胞凋... 详细信息

目的:观察针刀疗法治疗膝骨关节炎兔对其细胞凋亡的影响,探讨可能的作用机制。方法:正常新西兰兔随机分为:正常组、模型组、针刀组、中药组。左后膝伸直位石膏管型固定6周建立膝骨关节炎兔模型,原位末端标记(TUNEL)检测关节软骨细胞凋亡。结果:细胞凋亡指数正常组为(4.9±1.7),模型组为(25·2±4.3),针刀组为(11.6±3.1),中药组为(19.1±3.4)。模型组细胞凋亡指数显著升高,与正常组比较,经统计学处理,差异具有显著性意义(P<0.01)。针刀治疗和中药治疗均能降低细胞凋亡指数,两组比较,差异具有显著性意义(P<0.01)。结论:针刀疗法能抑制膝骨关节炎兔关节软骨细胞的凋亡,促进关节软骨形态恢复,这可能是针刀治疗膝骨关节炎的作用机制之一。

关键词：针刀疗法膝骨关节炎病理形态学细胞凋亡