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Chinese Journal of Chemistry 2021年第5期39卷 1288-1296页

作者： Zhi-Lin Luan Fei Qiao Wen-Yu Zhao Wen-Hua Ming Zhen-Long Yu jie liu Sheng-Yun Dai Shuang-Hui Jiang Chao-jie Lian Cheng-Peng Sun Bao-Jing Zhang Jian Zheng Shuang-Cheng Ma Xiao-Chi Ma Dalian Key Laboratory of Metabolic Target Characterization and Traditional Chinese Medicine Intervention College of PharmacyCollege of Integrative MedicineDalian Medical UniversityDalianLiaoning 116044China National Institutes for Food and Drug Control Beijing 102629China

The investigation of Morinda officinalis led to the isolation of twelve compounds(1-12),including three new iridoid glycosides morindallns A-C(1-3)and nine known compounds(4-12).Their structural identifications were conducted using HRMS,1D and 2D NMR,and electronic circular dichroism(ECD)spectra as well as quantum chemical *** 6 displayed the most significantly agonistic activity against farnesoid X receptor(FXR)with an EC_(50) value of 7.18 μM,and its agonistic effect was verified through the investigation of FXR downstream target genes including small heterodimer partner 1(SHP1),bile salt export pump(BSEP),and organic solute transporter subunit alpha and beta(OSTα and OSTβ).The potential interaction of compound 6 with FXR was analyzed by molecular docking and molecular dynamics stimulation,revealing that amino acid residues Leu287;Thr288,and Ser332 played a crucial role in the activation of compound 6 towards *** findings suggested that compound 6 could be regarded as a potential candidate for the development of FXR agonists.

关键词： Morinda officinalis Iridoid Farnesoid X receptor Agonistic effect

PlantscRNAdb: A database for plant single-cell RNA analysis

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Molecular Plant 2021年第6期14卷 855-857页

作者： Hongyu Chen Xinxin Yin Longbiao Guo jie Yao Yiwen Ding Xiaoxu Xu Lu liu Qian-Hao Zhu Qinjie Chu Longjiang Fan Institute of Crop Science and Institute of Bioinformatics Zhejiang UniversityHangzhou 310058China State Key Lab for Rice Biology China National Rice Research InstituteChinese Academy of Agricultural SciencesHangzhou 310006China CSIRO Agriculture and Food CanberraACT 2601Australia Shandong(Linyi)Institute of Modern Agriculture of Zhejiang University Fengyi StreetLinyiShandong ProvinceChina

Dear Editor,Many functional characteristics of plant tissue attribute to highly specialized cells in the *** cell types and profiling the transcriptome and proteome of individual cell types of plant tissues are important for understanding cellular activities underlying plant development and stress adaptation(Shaw et al.,2021).Single-cell RNA sequencing(scRNA-seq)provides such a tool for studying the transcriptional activities of individual cell types at single-cell resolution in any given organism(Rich-Griffin et al.,2020).

关键词： specialized cRNA

Further evidence of endogenous hydrogen sulphide as a mediator of relaxation in human and rat bladder

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Asian Journal of Andrology 2013年第5期15卷 692-696页

作者： Jun-Wei Gai Wasilijiang Wahafu Hui Guo Miao liu Xu-Chang Wang Yun-Xiang Xiao Liang Zhang Zhong-Cheng Xin jie Jin Department of Urology Peking University First Hospital Beijing 100034 China The Institute of Urology Peking University Beijing 100034 China Department of Urology PLA General Hospital Beijing 100853 China Department of Urology Tsinghua University First Hospital Beijing 100016 China Hedong Center for Disease Control and Prevention Tianjin 300151 China Department of Orthopaedic Surgery Beijing Jishuitan Hospital Fourth Clinical College of Peking University Beijing 100035 China Andrology Center Peking University First Hospital Beijing 100034 China

We investigated the expression of hydrogen sulphide （H2S） in human and rat lower urinary tract （including bladder, prostate and urethra） tissues, and we sought to determine whether H2S induces relaxation of human and Sprague-Dawley （SD） rat bladder strips. Human normal lower urinary tract tissue was obtained for the evaluation of endogenous H2S productivity using a sulphide-sensitive electrode and for the analysis of the expression levels of all three synthases of endogenous H2S, cystathionine β-synthase （CBS）, cystathionine y lyase （CSE） and 3-mercaptopyruvate sulphur transferase （MPST, as known as 3-MST） by Western blot assay. CBS, CSE and MPST were located in human sample slides by immunohistochemistry. Human and male adult SD rat bladder strips were tested for H2S function with a transducer and recorded. All experiments were repeated six times. The endogenous H2S productivity and the H2S synthases had various distributions in the human and rat lower urinary tract tissues and were located in both epithelial and stromal sections. L-cysteine （L-Cys, a substrate of CBS, CSE and MPST） elicited relaxation in a dose-dependent manner on human bladder strips ere-contracted by acetylcholine chloride. This effect could be diminished by the ATe-sensitive potassium ion （KATe） channel blocker glibenclamide （GLB）, the CSE inhibitor DL-propargylglycine （PEG） and the CBS inhibitor hydroxylamine （HA）. H2S and its three synthases were present in the human and rat lower urinary tract tissues and relaxed human and rat bladder strips, which implied that endogenous H2S might play a role in physiological function and pathological disorders of the lower urinary tract symptoms （LUTS） or overactive bladder （OAB）.

关键词： cystathionine β-synthase cystathionine γ lyase detrusor hydrogen sulphide lower urinary tract symptoms 3-mercapto- pyruvate sulphur transferase

Environmental hypoxia induces apoptosis in large yellow croaker Larimichthys crocea via both intrinsic and extrinsic pathways

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Journal of Oceanology and Limnology 2023年第6期41卷 2429-2443页

作者： Shengyu LUO Cheng liu Xinming GAO Jingqian WANG Yibo ZHANG jie DING Congcong HOU Junquan ZHU Bao LOU Weiliang SHEN Xiongfei WU Chundan ZHANG Key Laboratory of Applied Marine Biotechnology by the Ministry of Education School of Marine SciencesNingbo UniversityNingbo 315211China State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Zhejiang Academy of Agricultural SciencesHangzhou 310021China State Key Laboratory of Large Yellow Croaker Breeding Ningbo Academy of Oceanology and FisheryNingbo 315012China

Hypoxia has become an unfavorable factor affecting the sustainable development of the large yellow croaker Larimichthys crocea,an economically important mariculture fish in *** is a consequence of hypoxia on ***,the effects of hypoxia stress on apoptosis in *** remain largely *** investigated the effect of environmental hypoxia on apoptosis in *** show that hypoxia induced apoptosis in *** both in vivo and in *** mitochondrial membrane potential was significantly reduced in large yellow croaker fry(LYCF)*** expression levels of Bcell lymphoma/leukemia-2(Bcl-2)m RNA and protein were also significantly decreased in the liver and LYCF cells during 96 h and 48 h of hypoxia stress,respectively,whereas the expression level of Bcl-2 associated X(Bax)mRNA,Casp3 mRNA,and activity of caspase-3/7/9 were significantly increased,indicating that hypoxia induced caspase-dependent intrinsic apoptosis in *** expression level of the apoptosis-inducing factor(AIF)protein was significantly increased in the liver and LYCF *** level of AIF protein was significantly decreased in the cytoplasm but increased in the nuclei of ***,demonstrating that hypoxia induced the AIF-mediated caspase-independent intrinsic *** addition,the activity of caspase-8 was significantly increased,indicating that hypoxia stress induced extrinsic apoptosis in ***,hypoxia induced apoptosis in *** through both the intrinsic and extrinsic *** present study accumulated basic biological information to help elucidate the mechanism of hypoxia response in marine fish.

关键词： hypoxia large yellow croaker mitochondrial apoptosis pathway death receptor apoptosis pathway

Static-to-kinematic modeling and experimental validation of tendon-driven quasi continuum manipulators with nonconstant subsegment stiffness

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Chinese Physics B 2024年第1期33卷 316-326页

作者：郑先杰丁萌刘辽雪王璐郭毓 School of Automation Nanjing University of Science and TechnologyNanjing 210094China

Continuum robots with high flexibility and compliance have the capability to operate in confined and cluttered environments. To enhance the load capacity while maintaining robot dexterity, we propose a novel non-constant subsegment stiffness structure for tendon-driven quasi continuum robots(TDQCRs) comprising rigid-flexible coupling *** at real-time control applications, we present a novel static-to-kinematic modeling approach to gain a comprehensive understanding of the TDQCR model. The analytical subsegment-based kinematics for the multisection manipulator is derived based on screw theory and product of exponentials formula, and the static model considering gravity loading,actuation loading, and robot constitutive laws is established. Additionally, the effect of tension attenuation caused by routing channel friction is considered in the robot statics, resulting in improved model accuracy. The root-mean-square error between the outputs of the static model and the experimental system is less than 1.63% of the arm length(0.5 m). By employing the proposed static model, a mapping of bending angles between the configuration space and the subsegment space is established. Furthermore, motion control experiments are conducted on our TDQCR system, and the results demonstrate the effectiveness of the static-to-kinematic model.

关键词： static-to-kinematic modeling scheme tendon-driven quasi continuum robot nonconstant subsegment stiffness tension attenuation effect

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基于模型设计的自动定制IP策略

浙江大学学报（理学版） 2013年第6期40卷 627-633页

作者：刘杰福州大学物理与信息工程学院福建福州350002

为了克服传统手工定制IP的开发瓶颈,加快IP的设计与确认的进程.引入了基于模型的自动定制用户IP的新思想,给出了自动生成IP的完整工具链.即在System Generator可视化开发平台上,给出进行算法模型的功能验证、自动HDL代码的RTL级验证、定... 详细信息

为了克服传统手工定制IP的开发瓶颈,加快IP的设计与确认的进程.引入了基于模型的自动定制用户IP的新思想,给出了自动生成IP的完整工具链.即在System Generator可视化开发平台上,给出进行算法模型的功能验证、自动HDL代码的RTL级验证、定制IP前的硬件协同仿真,用户IP的自动生成及定制IP后的硬件测试等的方法.并以Fibonacci数列的IP定制为例,介绍了其IP的定制及软硬件验证与测试过程.

关键词：基于模型设计 SystemGenerator

Low Temperature Tetragonal Tungsten Bronze Oxides for Li-ion Storage

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Chemical Research in Chinese Universities 2024年第5期40卷 922-926页

作者： XU Chengxin ZHANG Wenda LOU Chenjie LI Chengyu XU Ligang SHI Yongchao liu jie LUO Huajie FU Jipeng KUANG Xiaojun TANG Mingxue Guangxi Key Laboratory of Electrochemical and Magnetochemical Function Materials College of Chemistry and BioengineeringGuilin University of TechnologyGuilin541006P.R.China Center for High Pressure Science and Technology Advanced Research Beijing100193P.R.China Guangxi Key Laboratory of Optic and Electronic Materials and Devices Key Laboratory of New Processing Technology for Nonferrous Metals and MaterialsMinistry of EducationCollege of Materials Science and EngineeringGuilin University of TechnologyGuilin541004P.R.China School of Materials Science and Engineering&Key Laboratory of Advanced Materials and Devices for Post-Moore Chips Ministry of EducationUniversity of Science and Technology BeijingBeijing100083P.R.China Key Laboratory of Rare Earth Optoelectronic Materials and Devices of Zhejiang Province Institute of Optoelectronic Materials and DevicesChina Jiliang UniversityHangzhou310018P.R.China

Nb-based tungsten bronze oxides have emerged as attractive materials in various fields,owing to the structural openings and simple synthesis *** this work,the tetragonal tungsten bronze(TTB)NaWNbO_(6) was prepared by solid state reaction at a relatively low temperature of 775°*** local structure was systematically studied by solid state nuclear magnetic resonance(SSNMR)with the aid of transition electronic microscopy(TEM).The analysis indicates that NaWNbO6 has pentagonal,square,and triangular ***,square tunnels were partly occupied(50%)by Na,which creates the ability for the Li-ion storage with a volumetric capacity of 210 A·h·L^(−1)at 0.2 *** 2D ^(23)Na-^(23)Na EXSY results further suggest the ability of ions to fast exchange between the tetragonal and pentagonal tunnels,resulting in a high-rate performance 20 C.

关键词： Tetragonal tungsten bronze Solid-state^(23)Na NMR ^(93)Nb NMR spectrum Fast ion exchange

A multiplex real-time PCR assay for simultaneous detection of classical swine fever virus,African swine fever virus,and atypical porcine pestivirus

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Journal of Integrative Agriculture 2023年第2期22卷 559-567页

作者： SONG Xiang-peng XIA Ying-ju XU Lu ZHAO Jun-jie WANG Zhen ZHAO Qi-zu liu Ye-bing ZHANG Qian-yi WANG Qin China Institute of Veterinary Drug Control/National Reference Laboratory for Classical Swine Fever Beijing 100081P.R.China

With the implementation of the C-strain vaccine,classical swine fever(CSF) has been under control in China,which is currently in a chronic atypical epidemic *** swine fever(ASF) emerged in China in 2018 and spread quickly across the *** is presently occurring sporadically due to the lack of commercial vaccines and farmers’ increased awareness of *** porcine pestivirus(APPV) was first detected in Guangdong Province,China,in 2016,which mainly harms piglets and has a local epidemic situation in southern *** three diseases have similar clinical symptoms in pig herds,which cause considerable losses to the pig *** are difficult to be distinguished only by clinical ***,developing an early and accurate simultaneous detection and differential diagnosis of the diseases induced by these viruses is *** this study,three pairs of specific primers and Taq-man probes were designed from highly conserved genomic regions of CSFV(5’ UTR),African swine fever virus(ASFV)(B646L),and APPV(5’ UTR),followed by the optimization of reaction conditions to establish a multiplex real-time PCR detection *** results showed that the method did not cross-react with other swine pathogens(porcine circovirus type 2(PCV2),porcine reproductive and respiratory syndrome virus(PRRSV),foot-and-mouth disease virus(FMDV),pseudorabies virus(PRV),porcine parvovirus(PPV),and bovine viral diarrhea virus BVDV).The sensitivity results showed that CSFV,ASFV,and APPV could be detected as low as 1 copy μL–1;the repeatability results showed that the intra-assay and interassay coefficient of variation of ASFV,CSFV,and APPV was less than 1%.Twenty-two virus samples were detected by the multiplex real-time PCR,compared with national standard diagnostic and patented method assay for CSF(GB/T 27540–2011),ASF(GB/T 18648–2020),and APPV(CN108611442A),*** sensitivity of this triple real-time PCR for CSFV,ASFV,and APPV was almost the

关键词： classical swine fever virus African swine fever virus atypical porcine pestivirus real-time PCR

High-Efficiency Reduction of Rice Amylose Content via CRISPR/Cas9-Mediated Base Editing

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Rice science 2020年第6期27卷 445-448页

作者： Li He Li Xiufeng Xu Yang liu Hualong He Mingliang Tian Xiaojie Wang Zhenyu Wu Xiuju Bu Qingyun Yang jie College of Life Science Northeast Agricultural UniversityHarbin 150030China Northeast Institute of Geography and Agroecology Key Laboratory of Soybean Molecular Design BreedingChinese Academy of SciencesHarbin 150081China Institute of Food Crops Jiangsu Academy of Agricultural SciencesNanjing 210014China Rice Research Institute College of AgricultureNortheast Agricultural UniversityHarbin 150030China

Rice is a staple food for more than half of the human *** has been estimated that by 2030,rice production must increase by 40%to meet the growing demand(Khush,2005).In addition,with the improvement of people's living standards,the demand for elite rice with better eating and cooking quality(ECQ)is *** is determined by several factors,including amylose content(AC),gel consistency(GC),gelatinization temperature(GT)and viscosity,where AC is the predominant factor(Juliano,1998).

关键词： CRISPR/Cas9 elite cooking

Discovery of C19-9 as a novel non-RGD inhibitor of αvβ3 to overcome enzalutamide resistance in castration-resistant prostate cancer

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Signal Transduction and Targeted Therapy 2023年第3期8卷 891-894页

作者： Xiaocong Pang Xiaojiao Sun Yanlun Gu Xu He Kan Gong Song Song Jixin Zhang jie Xia Zhenming liu Yimin Cui Peking University First Hospital Xishiku StreetXicheng District100034 BeijingChina School of Pharmaceutical Sciences Peking UniversityXueyuan Road 38Haidian District100191 BeijingChina Institute of Materia Medica Chinese Academy of Medical SciencesNanwei RoadXicheng District100050 BeijingChina

Dear Editor,The integrinαvβ3 receptor is a promising target for anticancer therapy.1,2 However,there are no effective marketed treatments targetingαvβ*** possible limitation of Arginine-Glycine-Aspartic(RGD)-mimeticαvβ3 antagonists has been shown to cause partial agonism,which could induce major conformational changes that trigger paradoxical cell adhesion and angiogenesis.

关键词： αvβ3 overcome resistance