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Development and characterization of novel polymorphic microsatellite markers for the Korean freshwater snail Semisulcospira coreana and cross-species amplification using next-generation sequencing

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Journal of Oceanology and Limnology 2020年第2期38卷 503-508页

作者： Yeon Jung PARK Mi Nan lee Eun-Mi KIM Jung Youn PARK Jae Koo NOH Tae-Jin CHOI Jung-Ha KANG Biotechnology Research Division National Institute of Fisheries ScienceBusan 46083Republic of Korea Department of Microbiology Pukyoung National UniversityBusan 48513Republic of Korea

Korean freshwater snails of the genus Semisulcospira are widely distributed across East *** has been a very popular nutritional food in Korea,and is an ecologically important water quality indicator because it lives only in clean ***,no microsatellite markers have been generated to study the population genetic diversity of this *** the present study,we developed and characterized 18 novel microsatellite loci from Semisulcospira coreana genomic *** microsatellites were isolated using 454 GS-FLX titanium sequencing and 18 markers were used for genotyping in *** addition,we also tested the cross-species transferability of the microsatellite markers in four additional Semisulcospira *** identified 18 polymorphic loci and the number of alleles per loci,and their polymorphism information content values ranged from 2 to 17 and 0.203 to 0.902,*** observed and expected heterozygosities of the loci ranged from 0.063 to 0.924 and 0.226 to 0.924,*** to the analysis of the cross-species transferability of these markers,four species,***,***,***,and ***,showed a very high transferability(80%–85%).These results show that this set of nuclear markers could be useful for population genetics studies of this species and closely related species.

关键词： Semisulcospira next-generation sequencing(NGS) microsatellite markers cross-species transferability

Comparison of the clinical efficacy of medical treatment of symptomatic benign prostatic hyperplasia between normal and obese patients

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Asian Journal of Andrology 2011年第5期13卷 728-731页

作者： Seung Hwan lee Cheol Young Oh Kyung Kgi Park Mutt Su Chung Se Jeong Yoo Byung Ha Chung Department of Urology Yonsei University Health System Seoul Korea Department of Urology Hallym University Anyang 431-070 Chuncheon

We aimed to investigate the difference in efficacy of medical treatment of symptomatic benign prostatic hyperplasia （BPH） between normal and obese patients with BPH; obesity was determined by either body mass index （BMI） or waist circumference （WC）. In this 12-week prospective observational study, a total of 175 patients aged ≥40 years with International Prostate Symptom Scores （IPSS） ≥12 points and prostate volume≥20 ml were prospectively enrolled. The patients were divided into two groups according to BMI or WC. Patients received the doxazosin gastrointestinal therapeutic system （GITS） at a dose of 4 mg once per day for 12 weeks. The changes from baseline in the IPSS, maximal urinary flow rate （Qmax）, post-void residual volume, quality of life （QoL） scores and adverse events （AEs） were analysed. Of the 175 enrolled patients, 132 completed the study. Sixty-seven patients had BMI 〉23 kg m-2, and 43 had WC 〉90 cm. Obese patients represented by WC 〉90 cm or BMI≥ 23 kg m-2 had a significantly greater prostate volume compared with non-obese patients at baseline. Total IPSS was significantly higher in the WC 〉90 cm group compared to the WC ≤ 90 cm group. Total I PSS was positively correlated with prostate volume （P=-0.031） and WC （P=0.045）. All groups showed significant improvements in total IPSS and QoL at 12 weeks. However, the improvement of total IPSS was greater in the high-BMI and high-WC groups. The most frequent AE was dizziness （n= 13）, and it was significantly lower in the obese BPH patients. Obesity was associated with increased prostate volume and lower urinary tract symptoms. Alpha-blockers appear to be efficacious for controlling symptoms, especially in obese men.

关键词： Alpha-blocker benign prostatic hyperplasia body mass index prostate waist circumference

Intrinsic electrochemical activity of Ni in Ni_(3)Sn_(4) anode accommodating high capacity and mechanical stability for fast-charging lithium-ion batteries

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Journal of Energy Chemistry 2022年第8期31卷 470-477,I0013页

作者： Janghyuk Moon Trung Dinh Hoang Seong Soo Park Seowan Park Dong Young Rhee Junwon lee Sang A Han Min-Sik Park Jung Ho Kim School of Energy Systems Engineering Chung-Ang UniversityHeukseok-RoDongjak-guSeoul 06974Republic of Korea Department of Advanced Materials Engineering for Information and Electronics Integrated Education Institute for Frontier Science&Technology(BK21 Four)Kyung Hee University1732 Deogyeong-daeroGiheung-guYongin 17104Republic of Korea Institute for Superconducting&Electronic Materials(ISEM) Australian Institute of Innovative Materials(AIIM)University of WollongongInnovation CampusSquires WayNorth WollongongNSW 2500Australia

Fast interfacial kinetics derived from bicontinuous three-dimensional(3D)architecture is a strategic feature for achieving fast-charging lithium-ion batteries(LIBs).One of the main reasons is its large active surface and short diffusion ***,understanding of unusual electrochemical properties still remain great challenge due to its *** this study,we proposed a nickel–tin compound(Ni_(3)Sn_(4))supported by 3D Nickel scaffolds as main frame because the Ni_(3)Sn_(4) clearly offers a higher reversible capacity and stable cycling performance than bare tin(Sn).In order to verify the role of Ni,atomic-scale simulation based on density functional theory systematically addressed to the reaction mechanism and structural evolution of Ni_(3)Sn_(4) during the lithiation *** findings are that Ni enables Ni_(3)Sn_(4) to possess higher mechanical stability in terms of reactive flow stress,subsequently lead to improve Li storage *** study elucidates an understanding of the lithiation mechanism of Ni_(3)Sn_(4) and provides a new perspective for the design of high-capacity and high-power 3D anodes for fast-charging LIBs.

关键词： Lithium-ion batteries Ni_(3)Sn_(4) High-capacity anode 3D-structured electrode Inverse opal structure Density functional theory

Comparison of Bacterial Profiles of Fish between Storage Conditions at Retails Using DGGE and Banding Pattern Analysis: Consumer’s Perspective

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Food and Nutrition Sciences 2012年第2期3卷 190-200页

作者： Chongtao Ge Chang Soo lee Zhongtang Yu Jiyoung lee College of Public Health Division of Environmental Health Sciences The Ohio State University Columbus USA Department of Animal Sciences The Ohio State University Columbus USA. Department of Food Science & Technology The Ohio State University Columbus USA Department of Food Science & Technology The Ohio State University Columbus USA 2College of Public Health Division of Environmental Health Sciences The Ohio State University Columbus USA

Bacterial profiles between storage conditions (frozen vs fresh) were compared using PCR-DGGE and banding pattern analysis. Salmon and tuna were collected and the bacteria cells were separated from fish cells using a somatic cell re-leasing agent. The results demonstrated that some were indigenous waterborne bacteria. However, the majority were identified as spoilage bacteria, pathogens and potential fecal contamination bacteria. Banding pattern analysis showed that the storage conditions were a significant factor in clustering bacteria. Frozen fish showed a smaller number of bac-terial species than fresh samples. Freezing seemed to play a role as a selective pressure by inactivating some microor-ganisms while favoring resistant ones to low temperature. The bacterial profiles seemed to be more influenced by afterharvest practices than the original environmental contamination. These findings provide consumers insight into fish quality, potential health risks of raw fish consumption, and the impact of storage conditions on bacterial group of raw fish.

关键词： Fish PCR-DGGE Bacterial Profiles Storage

Factors Affecting the Genetic Diagnostic Rate in Congenital Heart Disease

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Congenital Heart Disease 2022年第6期17卷 653-673页

作者： Jun Sung Park Go Hun Seo Yunha Choi Soojin Hwang Minji Kang Hyo-Sang Do Young-Hwue Kim Jeong Jin Yu Ellen Ai-Rhan Kim Euiseok Jung Byong Sop lee Jae Suk Baek Beom Hee lee Department of Pediatrics Asan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea 3 Billion Inc.SeoulKorea Genome Research Center for Birth Defects and Genetic Diseases Asan Institute for Life SciencesAsan Medical CenterSeoulKorea Division of Cardiology Department of PediatricsAsan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea Division of Neonatology Department of PediatricsAsan Medical CenterUniversity of Ulsan College of MedicineSeoulKorea Medical Genetics Center Asan Medical Center Children’s HospitalUniversity of Ulsan College of MedicineSeoulKorea

Background: Over 400 genes contribute to the development of congenital heart disease (CHD). Additionally,multisystemic manifestations accompanying syndromic CHD pose a higher risk of genetic diseases. This studyinvestigated the diagnostic yield of whole-exome sequencing (WES) in patients with sporadic syndromic CHDand the phenotypic factors affecting the genetic diagnostic rate. Methods: Sixty-four patients with sporadic syndromicCHD aged 0.05), betweenthe groups with and without a diagnostic variant. However, patients with ≥3 extracardiac phenotypes had a significantlyhigher likelihood of having a diagnostic variant than those with ≤2 (38.3% vs. 5.9%, odds ratio = 9.93,95% confidence interval = 1.21–81.44, P = 0.013). Conclusions: The number of extracardiac phenotypes is importantin predicting the possibility of genetic diagnosis. Physicians will be able to select patients with a high probabilityof genetic diagnosis and provide appropriate genetic counseling based on the results of this study.

关键词： Heart defects congenital whole-exome sequencing genetic testing phenotype

A Concept Compact City Vehicle Design for the Disabled Aging People

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Journal of Health Science 2015年第2期3卷 62-70页

作者： Wu Jun-Chieh lee Tsung-lee Chang Chien-Cheng Dept of Industrial Design Huafan University New Taipei City 223 Taiwan Dept of Industrial Design National United University Miaoli City 360 Taiwan

The degeneration of physical function of the aging often causes physical disability. The aging people have difficulties in getting in and out of the compact car, decreasing their chance to go outdoors. The present study is based on observation of six disabled aged users of light to moderate physical disability in moving inside and getting in and out of a car. From the video and interviews, problems the disabled aged people encountered in using compact cars were addressed. To solve the inconvenience and difficulty elderly disabled passengers have in a compact car, a concept design of a welfare vehicle was developed. In the concept welfare vehicle, a big hand bar on the side of the car door that can be controlled with the operation of car door was proposed to help the aging people support their body. Moreover, special enlarged chair, hand grip, and lighting under the rear seat were offered to help the aging people get in and out of the ear. In addition, a hand grip hidden on the ceiling can help the elderly disabled passengers move around inside a compact car.

关键词： Aging people disabled welfare vehicle design.

基于玻色凝聚^(87)Rb原子气体相位Ramsey干涉的磁场探测

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深圳大学学报（理工版） 2024年第5期41卷 517-523页

作者：吴嘉涛方瑞环韩成银马翥詹畅李懋捷黄嘉豪鹿博李朝红中山大学物理与天文学院量子工程与精密测量实验室广东珠海519082 深圳大学量子精密测量研究所物理与光电工程学院射频异质异构集成全国重点实验室广东深圳518060 粤港澳大湾区量子科学中心广东深圳518045

基于玻色凝聚原子气体的磁场探测可兼顾高灵敏度和高空间分辨率.利用一维光晶格中玻色凝聚^(87)Rb原子气体F=1,m_(F)=1→F=2,m_(F)=-1(F为原子总角动量的量子数,m_(F)为F能级的磁量子数)的双光子跃迁,已实现基于相位Ramsey干涉的精密磁... 详细信息

基于玻色凝聚原子气体的磁场探测可兼顾高灵敏度和高空间分辨率.利用一维光晶格中玻色凝聚^(87)Rb原子气体F=1,m_(F)=1→F=2,m_(F)=-1(F为原子总角动量的量子数,m_(F)为F能级的磁量子数)的双光子跃迁,已实现基于相位Ramsey干涉的精密磁场探测.本研究利用交叉光阱中玻色凝聚^(87)Rb原子气体F=1,m_(F)=1→F=2,m_(F)=0的单光子跃迁,实验研究了频域Ramsey干涉、时域Ramsey干涉及相位Ramsey干涉,并基于相位Ramsey干涉评估了静态磁场测量的灵敏度和空间分辨率.通过对比上述3种Ramsey干涉的实验结果,发现相位Ramsey干涉可有效避免频域Ramsey干涉条纹的畸变和时域Ramsey干涉的退相干效应,从而提高静态磁场测量的灵敏度和准确度.在无磁屏蔽环境中,利用体积为(1.31±0.03)×10^(-9)cm^(3)的玻色凝聚^(87)Rb原子气体,获得了130 nT/√Hz的灵敏度,通过压缩原子气体制备时间和提高信号积累时间,灵敏度有望得到进一步提升.

关键词：光学 Ramsey光谱光学泵浦原子激光冷却与囚禁玻色凝聚原子气体精密磁场探测

H_2S Sensing Characteristics of Thin Film SnO_2 Sensor with N_2 Treatment

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稀有金属材料与工程 2006年第A3期35卷 385-388页

作者： Jong-Won Kim Kwang-Hyun Yun Geon-Young Cha Gi-Hong Rue Duk-Dong lee Jeung-Soo Huh Department of Nano-Science and Technology Sangyuk-Dong 1370Bukgu DaeguKorea Department of Materials Science and Metallurgy Sangyuk-Dong 1370Bukgu DaeguKorea School of Electronic Engineering Kyungpook National UniversitySangyuk-Dong 1370Bukgu DaeguKorea Department of Nano-Science and Technology Department of Materials Science and MetallurgySangyuk-Dong 1370Bukgu DaeguKorea

SnO_2 thin film sensors were fabricated by a thermal evaporation *** sensors were heated for thermal *** high porosity,SnO_2 thin film sensors were treated in a N_2 *** sensors that were treated with O_2 after being treated with N_2 showed 70 % sensitivity for 1×10~ -6) of H_2S,which is higher than the sensors that were only treated with O_*** Ni metal,as a catalyst,was evaporated on the thin film Sn on the Al_2O_3 *** sensor was heated to grow the Sn nanowire in a tube furnace with N_2 *** nanowire was heated for *** sensitivity of SnO_2 nanowire sensor was measured for 500×10~ -9) of H_*** selectivity of the SnO_2 nanowire sensor was compared with the thin film and the thick film SnO_*** sensor was measured for H_2S,CO,and NH_3 in this study.

关键词： SnO2 gas sensor N2 nanowire H2S

Degradation of 2,4-dichlorophenoxyacetic acid by UV 253.7 and UV-H_(2)O_(2):Reaction kinetics and effects of interfering substances

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Emerging Contaminants 2019年第1期5卷 53-60页

作者： Asok Adak Indrasis Das Bijoli Mondal Suman Koner Pallab Datta lee Blaney Department of Civil Engineering Indian Institute of Engineering Science and Technology ShibpurHowrah711103India Department of Civil Engineering Indian Institute of Technology KharagpurKharagpur721302India Department of Civil Engineering Jalpaiguri Government Engineering CollegeJalpaiguri735102India Center for Healthcare Science and Technology Indian Institute of Engineering Science and Technology ShibpurHowrah711103India Department of Chemical Biochemical and Environmental EngineeringUniversity of Maryland Baltimore CountyUSA

This work investigates the degradation of 2,4-dichlorophenoxy acetic acid(2,4-D)using UV irradiation and the UV-H_(2)O_(2) advanced oxidation process(AOP).For UV irradiation at 253.7 nm,~66%degradation was observed for a fluence of 20 J cm^(-2) and the apparent fluence-based,pseudo-first-order rate constant for 2,4-D was 5.77(±0.66)×10^(-5) cm^(2) mJ^(-1).With the UV-H2O2 AOP,approximately 97%degradation was observed for a fluence of 700mJ cm^(-2).Due to production of hydroxyl radicals,the apparent fluencebased rate constant was 100 times higher than that for direct UV *** effects of H2O2 dose,initial 2,4-D concentration,and water quality parameters,including pH(4e8),alkalinity(0e5mM HCO3),nitrate concentration(0e1mMas NO3),and ionic strength(0e17mM as NaCl),were *** observed rate constants were dependent on pH,alkalinity,and nitrate *** degradation of 2,4-D by the UV-H2O2 systemwas also examined in a real surface *** observed fluence-based rate constant in the surface water matrix was 2.6(±0.3)×10^(-3) cm^(2) mJ^(-1),and this value was similar to a distilled water matrix containing the same alkalinity and *** addition,the biodegradability of UV and UV-H_(2)O_(2) treated wastewater increased with irradiation time,suggesting that transformation products can be degraded by biological *** on this study,the UV-H_(2)O_(2) process represents a viable treatment method to transform 2,4-D into benign products.

关键词： 2,4-D UV-253.7 UV-H_(2)O_(2) Advanced oxidation Hydroxyl radicals