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SlWRKY30 and SlWRKY81 synergistically modulate tomato immunity to Ralstonia solanacearum by directly regulating SlPR-STH2

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Horticulture Research 2023年第5期10卷 99-111页

作者： Fengfeng Dang Jinhui Lin Yajing Li Ruoyun Jiang Yudong Fang Fei Ding shuilin he Yanfeng Wang State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources College of Life SciencesSouth China Agricultural UniversityGuangzhou 510642China Shaanxi Key Laboratory of Chinese Jujube Yan’an UniversityYan’anShaanxi 716000China College of Agriculture Fujian Agriculture and Forestry UniversityFuzhou 350002China College of Horticulture Fujian Agriculture and Forestry UniversityFuzhou 350002China School of Life Sciences Liaocheng UniversityLiaocheng 252000China

Bacterial wilt is a devastating disease of tomato(Solanum lycopersicum)caused by Ralstonia solanacearum that severely threatens tomato *** III WRKY transcription factors(TFs)are implicated in the plant response to pathogen infection;however,their roles in the response of tomato to *** infection(RSI)remain largely ***,we report the crucial role of SlWRKY30,a group III SlWRKY TF,in the regulation of tomato response to ***30 was strongly induced by ***30 overexpression reduced tomato susceptibility to RSI,and also increased H2O2 accumulation and cell necrosis,suggesting that SlWRKY30 positively regulates tomato resistance to *** sequencing and reverse transcription–quantitative PCR revealed that SlWRKY30 overexpression significantly upregulated pathogenesis-related protein(SlPR-STH2)genes SlPR-STH2a,SlPR-STH2b,SlPR-STH2c,and SlPR-STH2d(hereafter SlPR-STH2a/b/c/d)in tomato,and these SlPR-STH2 genes were directly targeted by ***,four group III WRKY proteins(SlWRKY52,SlWRKY59,SlWRKY80,and SlWRKY81)interacted with SlWRKY30,and SlWRKY81 silencing increased tomato susceptibility to *** SlWRKY30 and SlWRKY81 activated SlPR-STH2a/b/c/d expression by directly binding to their *** these results together,SlWRKY30 and SlWRKY81 synergistically regulate resistance to RSI by activating SlPR-STH2a/b/c/d expression in *** results also highlight the potential of SlWRKY30 to improve tomato resistance to RSI via genetic manipulations.

关键词： directly synergistic immunity

Proteomic analysis of blast-resistant near-isogenic lines derived from japonica rice, var. Yunyin, infected with Magnaporthe oryzae

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Chinese Science Bulletin 2014年第32期59卷 4312-4322页

作者： Weimin Xue Xiaohui Mao Yidong Wei Lian Ling Yongsheng Zhu shuilin he Jianfu Zhang Huaan Xie Rice Research Institute Fujian Academy of Agricultural Sciences National Engineering Laboratory of Rice South Base of National Key Laboratory of Hybrid Rice of China College of Crop Science Fujian Agriculture and Forestry

Rice blast is one of the most devastating diseases in the world and outbreaks occur *** differences in protein expression between blast resistant and susceptible near-isogenic lines(NILs)of japonica rice *** infected with Magnaporthe oryzae were analyzed using proteomics,indicated that 67 different proteins were identified from 75 obtained proteins using the matrixassisted laser desorption/ionization time-of-flight mass spectrometry *** specific expression proteins,43 up-regulated proteins and 17 down-regulated proteins were identified among the 67 *** bioinformatical analysis demonstrated that these 67 different proteins were involved in many biological physiological processes including five proteins related to photosynthesis,25 proteins related to metabolism,six proteins related to antioxidants,10 proteins related to protein synthesis and modification,five proteins related to signal transduction,four proteins related to adversity stress and 12 non-functional *** identified proteins were directly or indirectly related to *** proteins related to different physiological processes were *** cDNA sequences were predicted and their expression patterns were analyzed using real-time PCR,demonstrated that the genes would response to *** and the response blindingly different between blast resistant and blast susceptible NILs.

关键词：蛋白质组学近等基因系稻瘟病菌 VAR 粳稻学分感染基质辅助激光解吸

Dilute Aqueous-Aprotic Electrolyte Towards Robust Zn-Ion Hybrid Supercapacitor with High Operation Voltage and Long Lifespan

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Nano-Micro Letters 2024年第9期16卷 1-12页

作者： shuilin Wu Yibing Yang Mingzi Sun Tian Zhang Shaozhuan Huang Daohong Zhang Bolong Huang Pengfei Wang Wenjun Zhang Key Laboratory of Catalysis and Energy Materials Chemistry of Ministry of Education&Hubei Key Laboratory of Catalysis and Materials Science South-Central Minzu UniversityWuhan 430074People’s Republic of China Department of Materials Science and Engineering and Center of Super‑Diamond and Advanced FilmsCity University of Hong Kong83 Tat Chee AvenueKowloon TongHong Kong SARPeople’s Republic of China Department of Applied Biology and Chemical Technology The Hong Kong Polytechnic UniversityHung HomKowloonHong Kong SARPeople’s Republic of China Key Laboratory of Photochemical Conversion and Optoelectronic Materials Technical Institute of Physics and ChemistryChinese Academy of SciencesBeijingPeople’s Republic of China

With the merits of the high energy density of batteries and power density of supercapacitors,the aqueous Zn-ion hybrid supercapacitors emerge as a promising candidate for applications where both rapid energy delivery and moderate energy storage are ***,the narrow electrochemical window of aqueous electrolytes induces severe side reactions on the Zn metal anode and shortens its *** also limits the operation voltage and energy density of the Zn-ion hybrid ***'water in salt'electrolytes can effectively broaden their electrochemical windows,but this is at the expense of high cost,low ionic conductivity,and narrow temperature compatibility,compromising the electrochemical performance of the Zn-ion hybrid ***,designing a new electrolyte to balance these factors towards high-performance Zn-ion hybrid supercapacitors is urgent and *** developed a dilute water/acetonitrile electrolyte(0.5 m Zn(CF_(3)SO_(3))_(2)+1 m LiTFSI-H_(2)O/AN)for Zn-ion hybrid supercapacitors,which simultaneously exhibited expanded electrochemical window,decent ionic conductivity,and broad temperature *** this electrolyte,the hydration shells and hydrogen bonds are significantly modulated by the acetonitrile and *** a result,a Zn-ion hybrid supercapacitor with such an electrolyte demonstrates a high operating voltage up to 2.2 V and long lifespan beyond 120,000 cycles.

关键词： Zn-ion supercapacitors Zn metal anode Electrolyte engineering Hydrogen bonds Solvation structures

Tunning the bandgap of MnO_(2)homojunction by building active high-index facet to achieve rapid electron transfer for enhanced photocatalytic sterilization

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Journal of Materials Science & Technology 2024年第1期168卷 265-275页

作者： Tao Zhang Bo Li Chaofeng Wang shuilin Wu Shengli Zhu Hui Jiang Yufeng Zheng Zhaoyang Li Zhenduo Cui Yu Zhang Paul K Chu Xiangmei Liu Biomedical Materials Engineering Research Center Hubei Key Laboratory of Polymer MaterialsMinistry-of-Education Key Laboratory for the Green Preparation and Application of Functional MaterialsSchool of Materials Science and Engineering Hubei UniversityWuhan 430062China School of health Science&Biomedical Engineering Hebei University of TechnologyTianjin 300401China School of Materials Science&Engineering Peking UniversityBeijing 100871China School of Materials Science&Engineering the Key Laboratory of Advanced Ceramics and Machining Technology by the Ministry of Education of ChinaTianjin UniversityTianjin 300072China Department of Orthopedics Guangdong Provincial People’s HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Department of Physics Department of Materials Science and Engineering and Department of Biomedical EngineeringCity University of Hong KongHong Kong 999077China

Photocatalysis has been a research hotspot in recent years,and the design and modification of photocat-alysts have been the key *** methods for designing photocatalysts,including constructing heterojunctions and homojunctions,have been developed on the basis of *** this study,two homojunctions of manganese dioxide(MnO_(2)),including a high-index crystal plane homojunction and a general homojunction,are prepared using a stepwise hydrothermal *** a capping agent,the high-index crystal surface of the MnO_(2)is *** is found that the electron transport efficiency be-tween the two components of the homojunction with high-index planes is higher and the adsorption capacity of the oxygen is stronger,which leads to higher photocatalytic *** addition,the newly designed high-index homojunction is used for the treatment of bacterial infections,and it kills Staphy-lococcus aureus(***)and Escherichia coli(***)at rates of 99.95%±0.04%and 99.31%±0.25%,*** also has excellent therapeutic effects on mouse wounds,which implies superb practical application *** work provides a new strategy for the improved design of homojunctions and the application of photocatalytic materials.

关键词： Photocatalysis Homojunction Bandgap High-index facet Antibacterial

辣椒非特异性脂质转移蛋白基因CaLTP1的克隆及表达分析

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热带作物学报 2012年第10期33卷 1784-1788页

作者：冯冬林赖燕何水林福建农林大学园艺学院福建福州350002 福建农业职业技术学院福建福州350007 福建农林大学生命科学学院福建福州350002

非特异性脂质转移蛋白(nsLTPs)是植物中大量存在的小分子脂类结合蛋白,具有多种生物学功能。从辣椒cDNA文库中筛选得到1个nsLTPs基因,命名为CaLTP1;序列分析结果表明:该基因全长cDNA序列为1 211 bp,推测编码1个包含129个氨基酸残基的前... 详细信息

非特异性脂质转移蛋白(nsLTPs)是植物中大量存在的小分子脂类结合蛋白,具有多种生物学功能。从辣椒cDNA文库中筛选得到1个nsLTPs基因,命名为CaLTP1;序列分析结果表明:该基因全长cDNA序列为1 211 bp,推测编码1个包含129个氨基酸残基的前体蛋白。CaLTP1蛋白含有典型的脂质转移蛋白N-端信号肽,保守的AAI结构域和半胱氨酸位点。预测CaLTP1蛋白含有多种功能位点,包括3个CK2磷酸化位点,3个N-豆蔻酰化位点和1个PKC磷酸化位点。实时荧光定量分析组织特异性表达模式表明,CaLTP1在辣椒茎、叶、花中表达量升高。

关键词：辣椒脂质转移蛋白(nsLTPs) 表达

辣椒CaDREB3基因的克隆和表达特性分析

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热带作物学报 2016年第12期37卷 2376-2383页

作者：冯冬林何水林赖燕福建农业职业技术学院福建福州350007 福建农林大学生命科学学院福建福州350002

从构建的辣椒c DNA文库中筛选阳性克隆,测序并获得辣椒CaDREB3基因,研究该基因的亚细胞定位、瞬时表达及组织表达特性,揭示CaDREB3的功能及其在植物抗逆过程中的分子机制。应用预测软件对其进行功能及生物信息学分析,利用q RT-PCR分析Ca... 详细信息

从构建的辣椒c DNA文库中筛选阳性克隆,测序并获得辣椒CaDREB3基因,研究该基因的亚细胞定位、瞬时表达及组织表达特性,揭示CaDREB3的功能及其在植物抗逆过程中的分子机制。应用预测软件对其进行功能及生物信息学分析,利用q RT-PCR分析CaDREB3基因的瞬时表达及在不同组织中的表达特性,并以基因枪轰击洋葱表皮的方法,对CaDREB3基因进行亚细胞定位分析。结果表明,辣椒CaDREB3长度为1 997 bp,含有1071bp的完整的开放阅读框,编码357个氨基酸,含有ERF亚族的AP_2/ERF保守结构域,与西红柿(NP_001234707.1)、马铃薯(AET99101.1)及拟南芥(NP_177931.1)具有较高的氨基酸相似性,其中与西红柿的相似性最高,达83%。亚细胞定位表明该基因位于细胞核,瞬时表达表明CaDREB3可以和GCC-box结合,q RTPCR检测表明,CaDREB3基因的相对表达量在辣椒茎、叶和果实中表达量较高。实验明确了辣椒CaDREB3基因亚细胞定位信息和组织表达情况,为进一步研究其功能及分子机制提供依据。

关键词：辣椒 CaDREB3 亚细胞定位瞬时表达荧光定量RT-PCR

Honeycomb-like biochar framework coupled with Fe_(3)O_(4)/FeS nanoparticles as efficient heterogeneous Fenton catalyst for phenol degradation

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Journal of Environmental Sciences 2024年第2期136卷 390-399页

作者： Aihua Cheng Yi he Xiaohe Liu Chi he Department of Environmental Engineering College of Geology and EnvironmentXi’an University of Science and TechnologyXi’an 710054China State Key Laboratory of Multiphase Flow in Power Engineering School of Energy and Power EngineeringXi’an Jiaotong UniversityXi’an 710049China National Engineering Laboratory for VOCs Pollution Control Material and Technology University of Chinese Academy of SciencesBeijing 101408China

Achieving an efficient and stable heterogeneous Fenton reaction over a wide pH range is of great significance for wastewater ***,a pollen-derived biochar catalyst with a unique honeycomb-like structure,coupled with the dispersion of magnetic Fe_(3)O_(4)/FeS(Fe/S)nanoparticles,was synthesized by simple impregnation precursor,followed by *** prepared Fe/S-biochar catalyst demonstrated outstanding phenol degradation efficiency across a wide pH range,with 98%of which eliminated even under neutral conditions(pH 7.0).The high catalytic activity was due to the multilevel porous structure of pollenderived biochar provided enough active sites and allowed for better electron transfer,then increases oxidation ability to promote the ***,the acid microenvironment formed by SO_(4)^(2-)group from Fe/S composite extended the pH range for Fenton reaction,and S^(2-)facilitated the conversion of≡Fe^(3+)to≡Fe^(2+),resulting in remarkable degradation ***,biochar can effectively promote cycling stability by limiting Fe *** work may provide a general strategy for designing 3D framework biochar-based Fe/S catalysts with excellent performance for heterogeneous Fenton reactions.

关键词： Biomass Biochar Fenton reaction Fe_(3)O_(4)/FeS(Fe/S) Phenol degradation

CaWRKY5,An Transcription Factor of Capsicum annuum,confer tobacco cold resistance by Overexpression

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CaWRKY5,An Transcription Factor of Capsicum annuum,confer to...

EGTA和La^(3+)对几种逆境诱导辣椒样半萜环化酶基因表达的影响

2008中国作物学会学术年会

作者： Wang Yuna Li Weihong Lin Guohua he shuilin (College of Life Science,Fujian Agriculture and Forestry University,Fuzhou 350002,P.R.China)

WRKYs is a plant specific gene family and most of them play important roles in plant stresses resistance, but the function and their molecular machenism of the most members in the family remained largly unknown, especially in the non model plants, such as pepper. In this report, a full length cDNA termed CaWRKY5 was isolated from a cDNA library of Capsicium annuurn, the results of subcellar licalizaion and DNA binding experiment showed that CaWRKY5 was nuclear-localized and and

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应用生态学报 2001年第2期12卷 272-274页

作者：何水林林文雄陈如凯福建农业大学作物学院福州350002

用钙离子螯合剂EGTA及细胞膜钙离子通道拮抗剂La3 +预处理辣椒叶片 ,以破坏辣椒叶片中的钙信使系统 ,再用紫外线、CuCl2 、HgCl2 处理辣椒叶片 ,研究表明EGTA和La3 +预处理未能降低CuCl2 、HgCl2 、UV诱导辣椒倍半萜环化酶活化的作用 ,E... 详细信息

用钙离子螯合剂EGTA及细胞膜钙离子通道拮抗剂La3 +预处理辣椒叶片 ,以破坏辣椒叶片中的钙信使系统 ,再用紫外线、CuCl2 、HgCl2 处理辣椒叶片 ,研究表明EGTA和La3 +预处理未能降低CuCl2 、HgCl2 、UV诱导辣椒倍半萜环化酶活化的作用 ,EGTA预处理反而对CuCl2 、HgCl2 、UV的诱导辣椒倍半萜环化酶活性作用有一定的促进效应 .单独用EGTA处理也能诱导离体辣椒叶片表现出倍半萜环化酶活性 .NorthenBlot分析结果表明 ,EGTA能诱导辣椒倍半萜环化酶基因转录 .研究表明 ,在辣椒倍半萜环化酶基因表达过程中 ,还存在钙信使系统以外的信号传递途径 ;非生物诱发因子对倍半萜环化酶基因表达诱导作用与生物Elicitor的诱导作用在信号传递上有差异 .

关键词：辣椒钙信使系统倍半萜环化酶基因表达非生物诱发因子逆境植物