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Detection and Identification of Avian, Duck, and Goose Reoviruses by RT-PCR: Goose and Duck Reoviruses Grouped the Same Specified Genogroup in Orthoreoviruses Genus SubgroupⅡ

Detection and Identification of Avian, Duck, and Goose Reovi...

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中国畜牧兽医学会家畜传染病学分会第六届全国会员代表大会暨第11次学术研讨会

作者： Yun Zhang Ming Liu shuidong ouyang Qilin Hu Zhou Han National Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences Virology Divisions Fujian Husbandry and Veterinary Research Institute Veterinary Department Northeast Agriculture University

A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck and goose reovirus (ARV, DRV and GRV) RNA from cell culture supernatant and clinical samples was established. The RNA of the virus was purified by the Trizol reagent and reverse transcription made by using random hexamers. The amplified, cloned, and sequenced 598-base pairs products on the σA-encoding gene fragment from 24 isolates (5 DRV, 3 GRV, and 8 ARV ranging more than 30 years) indicated that the primer regions were well conserved. The sensitivity of this method was determined to be 10 -2 PFU. Specificity of the RT-PCR method was determined by testing specimens containing avian influenza A viruses, newcastle disease virus, and Infectious bronchitis virus, all of which yielded negative results with no discernible background. The efficiency of the system for detection of ARV, DRV, and GRV directly in 71/83 clinical samples was confirmed. The nucleotide sequence analysis indicates that all the duck and goose reoviruses isolated from China different locale and year were closely related, showing 97.4-100 % identity to each other, but only with 86.7-88.5 % identity to French MDRV 89026. The homology of amplified σA-encoding gene and amino acids sequences in this study and GenBank were varied from 74.2-78.4% and 87.4-92.5%, respectively, between duck/geese and chicken species. Reoviruses σA-encoding gene segment shared 88.6-100% and 87.3-100% nucleotide identities, respectively, within duck/geese and chicken species. Phylogenetic analysis based on σA-encoding gene indicated that GRV with DRV segregated into the same specified genogroup within orthoreoviruses genus subgroup II and more related to ARV than to Nelson Bay virus (NBV). Overall, this study developed a sensitive and specific technique for the identification ARV, DRV and GRV and sequencing analysis enhanced our understudying of ARV, DRV, and GRV evolution relationship.

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Detection and Identification of Avian,Duck,and Goose Reoviruses by RT-PCR:Goose and Duck Reoviruses Grouped the Same Specified Genogroup in Orthoreoviruses Genus Subgroup Ⅱ

Detection and Identification of Avian,Duck,and Goose Reoviru...

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第六届全国会员代表大学暨第11次学术研讨会

作者： Yun Zhang, Ming Liu, shuidong ouyang, Qilin Hu, Zhou Han National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of ChineseAcademy of Agricultural Sciences, Harbin. 150001, P. R. ChinaVirology Divisions, Fujian Husbandry and Veterinary Research Institute, Fujian, 350003, P. R. China Veterinary Department, Northeast Agriculture University, Harbin, 150030, P. R. China

A reverse transcription-polymerase chain reaction (RT-PCR) procedure for the detection of avian, duck and goose reovirus (ARV, DRV and GRV) RNA from cell culture supernatant and clinical samples was established. The RNA of the virus was purified by the Trizol reagent and reverse transcription made by using random hexamers. The amplified, cloned, and sequenced 598-base pairs products on the σA-encoding gene fragment from 24 isolates (5 DRV, 3 GRV, and 8 ARV ranging more than 30 years) indicated that the primer regions were well conserved. The sensitivity of this method was determined to be 10-2 PFU. Specificity of the RT-PCR method was determined by testing specimens containing avian influenza A viruses, newcastle disease virus, and Infectious bronchitis virus, all of which yielded negative results with (?)discernible background. The efficiency of the system for detection of ARV, DRV, and GRV directly in 71/83 clinical samples was confirmed. The nucleotide sequence analysis indicates that all the duck and goose reoviruses isolated from China different locale and year were closely related, showing 97.4-100 % identity to each other, but only with 86.7-88.5 % identity to French MDRV 89026. The homology of amplified σA-encoding gene and amino acids sequences in this study and GenBank were varied from 74.2-78.4% and 87.4-92.5%, respectively, between duck/geese and chicken species. Reoviruses σA-encoding gene segment shared 88.6-100% and 87.3-100% nucleotide identities, respectively, within duck/geese and chicken species, Phylogenetic analysis based on σA-encoding gene indicated that GRV with DRV segregated into the same specified genogroup within orthoreoviruses genus subgroup 11 and more related to ARV than to Nelson Bay virus (NB V). Overall, this study developed a sensitive and specific technique for the identification ARV, DRV and GRV and sequencing analysis enhanced our understudying of ARV, DRV, and GRV evolution relationship.

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成分对水性醇酸树脂性能影响的研究

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广东化工 2014年第24期41卷 177-178页

作者：林水东童曼霞李小淳欧阳青海何立芳龙岩学院化学与材料学院福建龙岩364012

通过对桐油、中和剂、助溶剂和苯乙烯对水性醇酸树脂性能影响的研究,发现在用豆油油酸为原料,加入7%的桐油,用三乙胺作中和剂,用乙二醇丁醚作助溶剂,制取水性醇酸树脂,再用10%苯乙烯进行改性后,可得到水溶性、稳定性、干燥性、耐水性及... 详细信息

通过对桐油、中和剂、助溶剂和苯乙烯对水性醇酸树脂性能影响的研究,发现在用豆油油酸为原料,加入7%的桐油,用三乙胺作中和剂,用乙二醇丁醚作助溶剂,制取水性醇酸树脂,再用10%苯乙烯进行改性后,可得到水溶性、稳定性、干燥性、耐水性及粘度优良的水性醇酸树脂。

关键词：成分苯乙烯水溶性醇酸树脂性能

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种子表型平台的改进及其在芥菜种子活力研究中的应用

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种子 2023年第5期42卷 140-147页

作者：陆光远游永全刘羽婷李春生韦明肯欧阳乐军 Steven Penfield 赵永国广东石油化工学院生物与食品工程学院广东茂名525000 湖北工程学院湖北孝感432000 Department of Crop Genetics John Innes CentreNorwich NR47UHUK

为了研究芥菜萌发期的耐盐性,本研究通过增加喷雾装置等方面的改进,构建了新一代的高通量、低成本种子表型平台SeedGerm V 2.0,可实现无人值守运行。基于该种子表型平台,分析了地方特色芥菜品种水东甜芥菜在不同浓度NaCl胁迫下的种子萌... 详细信息

为了研究芥菜萌发期的耐盐性,本研究通过增加喷雾装置等方面的改进,构建了新一代的高通量、低成本种子表型平台SeedGerm V 2.0,可实现无人值守运行。基于该种子表型平台,分析了地方特色芥菜品种水东甜芥菜在不同浓度NaCl胁迫下的种子萌发动力学特征。结果表明,种子平台能够自动采集芥菜种子萌发数据并进行分析,可以用于盐胁迫研究。较低NaCl溶液对芥菜种子萌发率有一定促进作用,但随着盐浓度的升高,芥菜种子的萌发率迅速下降,浓度达到0.300 mol/L时发芽基本停止,表明其具有较强的耐盐性。估算了芥菜种子萌发的积盐模型(Halothermal time,HaloTT)参数,其中积盐常数θ_(halo)为2.26 mol/(L·h),中位值NaCl_(b(50))为0.22 mol/L,标准方差δ_(NaCl b)为0.067 mol/L。利用上述参数得到水东甜芥菜种子萌发预测方程:Probit(g)=(NaCl+2.26/t g-0.22)/0.067,可以准确预测不同盐浓度下芥菜种子的发芽时间和发芽率,对于芥菜的育种和生产具有重要参考价值。

关键词：芥菜种子平台种子萌发积盐模型盐胁迫

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