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Expression pattern of a β_2 tubulin gene in zebrafish embryos identified by whole mount in situ hybridization

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Chinese Science Bulletin 1998年第22期43卷 1894-1898页

作者： LI shuhong, GUO Shaodong and sun Fangzhen Institute of Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China Institute of Developmental Biology Chinese Academy of Sciences Beijing China

Zebrafish is a good vertebrate model for development studies. Research on gene expression and regulation during zebrafish embryonic development plays an important role in revealing the molecular mechanism of morphogenesis in vertebrate. Tubulin locates the cytoplasmic determinants in eggs, organizes the formation of basic cytoskeleton and controls various cell movements. There are several types of tubulin, each of which has specific expression regions and functions. In this study, the full_length cDNA of a Xenopus β 2 tubulin was used as template to generate Dig_labeled antisense RNA which was used as a probe to carry out in situ hybridization on whole mount zebrafish embryos. Under the experimental conditions, the tubulin transcript was first detected in a group of cells in embryos at the midblastula stage. With the development of the embryo, this transcript was gradually restricted to the nervous system. These results demonstrate that the expression of this β 2 tubulin gene in zebrafish embryos is neural specific.

关键词： tubulin gene whole mount in situ hybridization zebrafish.

Mixed infection of avian hepatitis E virus and Marek's disease virus in hens in China

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Mixed infection of avian hepatitis E virus and Marek's disea...

根系分区交替滴灌条件下葡萄根系分布特征及生长动态

第四届全国禽病分子生物技术青年工作者会议

作者： Shuqing Yang shuhong sun Shan Dong Agricultural University the Shandong Agricultural University

The average mortality of a flock of Hy-Line variety brown hens in Jiangsu Province,China,was 0.44%over ***-mortem inspection revealed that the Pathological changes was consistent with hepatitis-splenomegaly syndrome,including hepatomegaly,serosanguineous peritoneal fluid and hepatic subcapsular *** lesions of the liver were characterized by multifocal necrotizing hepatitis and mild lymphocytic *** of avian HEV RNA samples were detected from 56%to 80%of the liver from clinical onset chickens(14/25)and clinical healthy chickens(4/5)by polymerase chain reaction(PCR).Sequencing analysis of a 242-base-pair gene fragment from clinical onset chicken and clinical healthy chicken showed 97.9%to99.2%nucleotide sequence identity with avian HEV genomes,whereas identities ranged from 77.3%to 97.9%compared with other representative *** addition,40%to 48%of liver samples from clinical healthy chickens(2/5)and clinical onset chickens(12/25)were detectable amounts of MDV meq DNA by PCR,*** analysis of a 396-base-pair gene fragment showed that the MDV strain belonged to serotype 1 *** co-infection rate of avian HEV and MDV was 30%(9/30).In contrast to other reported avian HEV outbreaks that occurred in broiler breeder in China,in the present investigation clinical avian HEV was identified in Hy-Line variety brown *** study first found and reported the mixed infection of MDV and avian HEV in chicken.

关键词： Avian HEV MDV Co-infection Hy-Line variety brown hens.

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农业机械学报 2011年第9期42卷 59-63,58页

作者：周青云王仰仁孙书洪天津农学院水利工程系天津300384

采用原位取土法和根系生态监测系统连续两年研究了葡萄的根系空间分布和全生育期根系生长动态,结果表明:葡萄根系在水平方向主要分布在距离树干100 cm的范围内,占到总根系的80%以上,而且在径向方向呈指数衰减;葡萄根系在垂直方向主要分... 详细信息

采用原位取土法和根系生态监测系统连续两年研究了葡萄的根系空间分布和全生育期根系生长动态,结果表明:葡萄根系在水平方向主要分布在距离树干100 cm的范围内,占到总根系的80%以上,而且在径向方向呈指数衰减;葡萄根系在垂直方向主要分布在0～60 cm范围的土层内,占到总根系的75%以上。根系分区交替滴灌条件下干燥区与湿润区根系生长是不同的,葡萄的新生根系受到土壤水分条件的限制和自身生长的影响。在整个生育期,葡萄根系分区交替滴灌两侧根系生长均呈抛物线变化。

关键词：交替灌溉葡萄根系生长动态微根管

Effects of dietary supplementation of Clostridium butyrtcum on growth performance,immune response,intestinal microbiota in broiler chickens challenged with Salmonella enteritidis

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Effects of dietary supplementation of Clostridium butyrtcum ...

Polylysine-modified DNA vaccine showed elevated prophylactic and therapeutic effects in peanut allergy in mice

中国畜牧兽医学会动物微生态学分会第五届第十三次全国学术研讨会

作者： Zijing Ju Chengfang Men Yu Li shuhong sun College of Animal Science and Technology Shandong Agricultural University

Introduction Salmonella was one of the most important food-borne pathogens worldwide and caused tremendous economic losses in poultry *** was estimated that approximately 70%-80%of food-borne bacterial outbreaks were caused by Salmonella in *** were used to control Salmonella in the past decades,however,due to the emergence and of antimicrobial resistance,the use of probiotics

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Polylysine-modified DNA vaccine showed elevated prophylactic...

第十一届全国免疫学学术大会

作者： Zhu Zhenlai sun shuhong Niu Yuan Li Wei Department of Dermatology Xijing HospitalFourth Military Medical University

Objective:The prevalence of food allergy has been increasing,but the treatment is very *** vaccination hasbeen recognized as a promising method for the treatment of allergic diseases;however,poor immunogenicity has hindered its *** the present study,we assessed the prophylactic and therapeutic effects of poly-L-lysine(PLL)-pretreated DNA vaccine encoding the major peanut allergen Ara h 2 in ***:BALB/c mice were intradermally injected with plasmid DNA encoding the peanut protein Ara h 2(p Ara h 2) or p Ara h 2 pre-treated with PLL before or after sensitization with Ara h 2 *** h 2-specific antibodies were measured by *** cells(LCs) and Treg cells in draining lymph nodes were analyzed by flow cytometry after DNA immunization,and IL-10 production in splenocytes was also ***:In the prophylactic study,pre-treatment with p Ara h 2 or PLL-p Ara h 2 resulted in lower level of Ara h 2-specific Ig G1,Ig G2 a and Ig E after sensitization with Ara h 2 protein,and mice in PLL-p Ara h 2 group showed significantly lower level of antibodies than that of p Ara h *** the treatment study,intradermal injection with p Ara h 2 or PLL-p Ara h 2 after Ara h 2 protein sensitization significantly decreased the level of Ara h 2-specific antibodies,and PLL-p Ara h 2 had stronger effects than p Ara h *** were increased numbers of LCs and Treg cells in the mice receiving intradermal injection with PLL-p Ara h 2,and splenocytes from PLL-p Ara h 2-treated mice secreted increased level of ***:Modification of pA ra h 2 with PLL improved its prophylactic and therapeutic effects in peanut allergy in mice.

关键词： Ara h 2 Allergy vaccines Peanut allergy Polylysine Regulatory T cells

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微波法制备铜锌锡硫的研究进展

材料导报 2019年第13期33卷 2159-2166页

作者：沈韬柴鲜花孙淑红朱艳昆明理工大学信息与自动化工程学院昆明650093 昆明理工大学材料科学与工程学院昆明650093 中国材料学会青年工作委员会

尽管以铜铟镓硒(CIGS)和碲化镉(CdTe)为代表的第二代薄膜太阳能电池已成功实现商业化,截至目前,以CIGS为吸收层的电池的效率已经突破23%,但由于In元素稀缺,Cd元素有剧毒,限制了该电池的大规模生产。铜锌锡硫(Cu 2ZnSnS 4,CZTS)是一种直... 详细信息

尽管以铜铟镓硒(CIGS)和碲化镉(CdTe)为代表的第二代薄膜太阳能电池已成功实现商业化,截至目前,以CIGS为吸收层的电池的效率已经突破23%,但由于In元素稀缺,Cd元素有剧毒,限制了该电池的大规模生产。铜锌锡硫(Cu 2ZnSnS 4,CZTS)是一种直接带隙半导体,因具有禁带宽度合适、光吸收系数高、组分无毒和储量丰富等优点,而成为目前太阳能电池中最具有潜力的吸收层材料之一。目前以CZTS为吸收层的太阳能电池的效率已突破12%,已接近于商业化的多晶硅太阳能电池的效率。目前,CZTS粉体的制备方法主要为以溶剂热法、一锅法和热注入法为代表的溶液法。由于这些方法需要昂贵的仪器设备、复杂的操作顺序、较长的反应时间,且易产生杂相,严重制约着生产效率的提高。微波法也是一种溶液化学反应法,由于具有反应速度快、操作简单、效率高、加热均匀、能够减小热梯度等优势,近年在太阳能电池材料制备领域引起了广泛关注。微波法制备CZTS薄膜通常有一步成膜和两步成膜两种途径。两步成膜法先通过微波合成CTZS的粉体,再将粉体分散之后通过旋涂等方法获得CZTS的薄膜。采用这种方法得到的薄膜更加均匀、致密、稳定。然而,CZTS属于四元化合物,化学计量比的少量偏离就很容易产生其他杂相,因此如何减少和控制杂相的生成,制备纯相、形貌可控的CZTS纳米晶体并将其应用于薄膜太阳能电池显得至关重要。最近几年,除了研究CZTS对器件性能的影响外,研究者们主要从选择合适的有机溶剂、原料配比、反应时间、反应温度及表面活性剂等制备工艺方面不断尝试,并取得了丰硕的成果,在充分发挥微波法反应速度快、操作简单、效率高、加热均匀优势的同时大幅提升了器件效率。目前,以微波法合成的CZTS为吸收层材料制备的太阳能电池的转换效率已从最初的0.25%快速提高到4.92%。本文综述了近年来微波法制备CZTS粉末和CZTS薄膜的主要方法,总结了原料配比、溶剂、反应温度、反应时间和表面活性剂等对产物形貌、结构、光学性能等的影响,并对微波法制备CZTS的发展前景进行了展望,以期为制备更高转换效率的CZTS基太阳能电池提供参考。

关键词：微波 Cu2ZnSnS4 粉体薄膜

山东省某地鹦鹉喙羽病病毒的全基因组序列及对SPF鸡胚的致病性探究

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野生动物学报 2019年第3期40卷 670-678页

作者：吴少鹏宋艳孙淑红山东农业大学动物科技学院

2017年4月,山东省某鹦鹉场发生疑似喙羽病感染,鹦鹉出现异形喙和羽毛脱落,甚至急性猝死。目的:了解该地区鹦鹉喙羽病病毒( Psittacine beak and feather disease,PBFD )的流行现状、分子生物学特征和遗传变异规律,为PBFDV的防控提供参... 详细信息

2017年4月,山东省某鹦鹉场发生疑似喙羽病感染,鹦鹉出现异形喙和羽毛脱落,甚至急性猝死。目的:了解该地区鹦鹉喙羽病病毒( Psittacine beak and feather disease,PBFD )的流行现状、分子生物学特征和遗传变异规律,为PBFDV的防控提供参考依据。方法:收集怀疑有喙羽病病毒( PBFDV )感染的非洲灰鹦鹉的新鲜肝脏组织和羽毛样本,根据PBFDV全基因组序列设计特异性引物,从患病灰鹦鹉肝脏和羽毛组织中扩增PBFDV的全基因组序列,克隆到pMD19-T载体中,经测序获得PBFDV全长cDNA序列,利用DNAstar软件对全基因组进行遗传变异分析。由于目前尚未发现适合PBFDV繁殖的培养细胞或胚胎,我们尝试使用患病鹦鹉的羽毛研磨液注入7日龄SPF鸡胚的卵黄膜中。结果:分离到3株PBFDV ( GenBank登录号:MH279594,MH180298和MH190788 ),全基因组序列测序后,发现它们彼此具有93.3%—99.9%的相似性,并且它们也与GenBank中的一些参考序列具有86.1%—98.8%的相似性。卵黄囊注射病毒液后胚胎发育变缓,48—72 h内鸡胚死亡,全身出血。接着我们收集鸡胚的肝组织的研磨液在鸡胚上传代至第5代,均能使鸡胚死亡。提取来自每一代鸡胚肝组织的DNA都可以通过PCR扩增出PBFDV的目的条带。结论:以上实验结果表明,成功从患病鹦鹉体内克隆出PBFDV的全基因,并完成了序列分析,同时PBFDV接种鸡胚卵黄膜后成功复制。我们针对在该地区新出现的病毒从分子方面进行相关研究,分析其遗传变异特征,以研究该病毒与其他地区喙羽病病毒之间的亲缘关系,这些数据为研究该病毒的遗传变异和流行病学提供分子生物学依据。

关键词：喙羽病病毒全基因遗传分析

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基于黑臭水体治理方案研究及效果分析

水处理技术 2019年第10期45卷 137-140页

作者：赵鹏孙书洪天津农学院水利工程学院天津市节水灌溉技术与装备校企协同创新实验室天津市农业水利技术工程中心天津300384

以天津市大沽排水河黑臭水体为研究对象,采取分级处理措施:一级采用孔板式细格栅、沉砂池和沉淀池进行初步栅渣、沉砂和污泥处理,二级运用生物膜-膜生物反应器(复合MBR)处理。结果表明,COD和NH3-N、TP的质量浓度由最初的57.13 mg/L和8.8... 详细信息

以天津市大沽排水河黑臭水体为研究对象,采取分级处理措施:一级采用孔板式细格栅、沉砂池和沉淀池进行初步栅渣、沉砂和污泥处理,二级运用生物膜-膜生物反应器(复合MBR)处理。结果表明,COD和NH3-N、TP的质量浓度由最初的57.13 mg/L和8.82、2.18 mg/L分别下降到36.61 mg/L和3.55、0.48 mg/L,去除率分别达到35.92%和59.75%、77.98%。NH3-N、TP含量满足初期设计出口水质指标,COD达到GB 3838-2002的V类水标准。该装置体积小、整体性强、工作压力小且节能,实现了河道的优化管理。

关键词：黑臭水体生物膜-膜生物反应器综合水质标识指数

NONTRIVIAL SOLUTION OF A NONLINEAR SECOND-ORDER THREE-POINT BOUNDARY VALUE PROBLEM

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Applied Mathematics(A Journal of Chinese Universities) 2007年第1期22卷 37-47页

作者： Li shuhong sun Yongping Department of Mathematics, Lishui University, Lishui 323000,China Department of Applied Mathematics, Zhejiang Sci-Tec University, Hangzhou 310018, China Department of Electron and Information, Zhejiang University of Media and Communications, Hangzhou 310018, China. Department of Mathematics Lishui University Lishui China Department of Applied Mathematics Zhejiang Sci-Tec University Hangzhou China Department of Electron and Information Zhejiang University of Media and Communications Hangzhou China

In this paper, for a second-order three-point boundary value problem u″＋f（t,u）=0,0〈t〈1,au（0）-bu′（0）=0,u（1）-au（η）=0,where η∈ （0, 1）, a, b, α ∈R with a^2 ＋ b^2 〉 0, the existence of its nontrivial solution is studied. The＇conditions on f which guarantee the existence of nontrivial solution are formulated. As an application, some examples to demonstrate the results are given.

关键词： three-point boundary value problem nonlinear alternative of Leray-Schauder, nontrivial solution.