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Responses of phytoplankton and its satellite bacteria to exogenous ethanol

Journal of Oceanology and Limnology 2023年第1期41卷 203-214页

作者： Rediat ABATE Buce Hanoch HETHARUA Vishal PATIL Daner LIN Demeke KIFLE Junrong LIANG Changping CHEN Lin SUN shuh-ji kao Yonghong BI Bangqin HUANG Yahui GAO Key Laboratory of Algal Biology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China School of Life Sciences Xiamen UniversityXiamen 361102China Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystem College of Environment&EcologyXiamen UniversityXiamen 361102China State Key Laboratory of Marine Environmental Sciences Xiamen UniversityXiamen 361101China Department of Zoological Sciences Addis Ababa UniversityAddis AbabaPO Box 1176Ethiopia

The response of phytoplankton and its satellite bacteria to various concentrations(0.01%-10%v/v)of ethanol is *** elucidate the effect of ethanol,single-strains of phytoplankton(SSP)culture,pure strains of satellite bacteria isolated from nonaxenic SSP cultures,and Escherichia coli were *** indicate that ethanol could promote the growth and photo synthetic efficiency(F_(v)/F_(m))of S SP at 0.01%and the growth of satellite bacteria at 0.01%-1%.Nevertheless,ethanol inhibited the growth and F_(v)/F_(m)of SSP at 0.1%-1%,and killed bacteria and SSP at 10% *** investigation on a satellite bacterium(Mameliella alba)revealed that ethanol promotes growth by serving as a growth stimulant rather than a metabolic carbon *** 16 S rRNA gene amplicon indicated that all nonaxenic S SP cultures harbor distinct satellite bacteria communities where the SSP culture of Skeletonema costatum,Phaeodactylum tricornutum,and Dunaliella bardawil were dominated by bacteria genera of Marivita(~80%),Dinoroseobacter(~47%),and Halomonas(~87%),respectively,indicating that every SSP cultures have their own distinct satellite bacterial *** bacteria family Rhodobacteraceae was dominant in the two marine diatoms,whereas Halomonadaceae was dominant in the saline green *** to their respective controls,the supply of 0.5% ethanol to SSP cultures promoted the growth of the satellite bacteria but did not cause a significant difference in species composition of satellite ***,a low concentration of ethanol can promote the growth of bacteria in a non-selective *** study enriched our knowledge about the effect of ethanol on aquatic microbes and provided a baseline for basic and applied biotechnological re search in the aquatic environment in the future.

关键词： aquatic microbes bacteria diversity ethanol effect growth inhibition growth stimulation

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Evaluation of Atmospheric PCDD/F Depositions via Automated and Traditional Water Surface Samplers in Taiwan

Evaluation of Atmospheric PCDD/F Depositions via Automated a...

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第九届海峡两岸气溶胶技术研讨会暨第二届海峡两岸环境保护双门论坛

作者： Kai Hsien Chi shuh ji kao Shu Hao Chang Moo Been Chang Institute of Environmental and Occupational Health Sciences Taiwan Yang Ming University Research Center for Environmental Changes Academia Sinica Graduate Institute of Environmental Engineering Taiwan Central University

Polychlorinated dibenzo-p-dioxins and dibenzofurans(PCDD/Fs)are a group of compounds of major environmental *** emitted into the atmosphere,PCDD/Fs undergo photochemical reactions and enter other environmental compartments via wet and dry *** this study,atmospheric PCDD/F depositions were collected via an automated PCDD/F deposition sampler and traditional cylindrical vessels,respectively,in northern,central and southern Taiwan from 2008 to *** automated PCDD/F precipitation sampler used in this study can prevent both re-suspension and photodegradation of the PCDD/Fs collected and also effectively separates the PCDD/F samples into dry and wet *** results indicate that the average atmospheric PCDD/F concentrations collected by the high-volume sampling trains were:13.6±10(n=10),15.6+5.2(n=7)and 10.9+6.3(n=6)fg I-TEQ/m in northern,central and southern Taiwan,*** addition,the results also indicate that the PCDD/F deposition flux collected with an automated PCDD/F sampler(1.84±0.90.68±5.1 pg I-TEQ/m/day,n=23)is significantly higher than that sampled with cylindrical vessels(1.11±0.69.64±5.2 pg I-TEQ/m/day,n=23).Based on the Mann-Whitney statistical analysis,the p value(0.037)of PCDD/F deposition flux between those two samplers measurement is lower than *** difference is attributed to the fact that part of the PCDD/F depositions collected by traditional cylindrical vessels is photo degraded and re-volatilization.

关键词： Dioxins deposition water surface sampler aerosol

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An Improved Training Algorithm Based on Ensemble Penalized Cox Regression for Predicting Absolute Cancer Risk

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China CDC weekly 2023年第9期5卷 206-212,I0009-I0014页

作者： Liyuan Liu Fu Yang Yeye Fan Chunyu kao Fei Wang Lixiang Yu Yong He jiadong ji Zhigang Yu Department of Breast Surgery The Second HospitalCheeloo College of MedicineShandong UniversityJinan CityShandong ProvinceChina School of Mathematics Shandong UniversityJinan CityShandong ProvinceChina Zhongtai Securities Institute for Financial Studies Shandong UniversityJinan CityShandong ProvinceChina Institute of Translational Medicine of Breast Disease Prevention and Treatment Shandong UniversityJinan CityShandong ProvinceChina.

Introduction:Biases in cancer incidence characteristics have led to significant imbalances in databases constructed by prospective cohort *** they use imbalanced databases,many traditional algorithms for training cancer risk prediction models perform ***:To improve prediction performance,we introduced a Bagging ensemble framework to an absolute risk model based on ensemble penalized Cox regression(EPCR).We then tested whether the EPCR model outperformed other traditional regression models by varying the censoring rate of the simulated ***:Six different simulation studies were performed with 100 *** assess model performance,we calculated mean false discovery rate,false omission rate,true positive rate,true negative rate,and the areas under the receiver operating characteristic curve(AUC)*** found that the EPCR procedure could reduce the false discovery rate(FDR)for important variables at the same true positive rate(TPR),thereby achieving more accurate variable *** addition,we used the EPCR procedure to build a breast cancer risk prediction model based on the Breast Cancer Cohort Study in Chinese Women *** for 3-and 5-year predictions were 0.691 and 0.642,representing improvements of 0.189 and 0.117 over the classical Gail model,***:We conclude that the EPCR procedure can overcome challenges posed by imbalanced data and improve the performance of cancer risk assessment tools.

关键词： Cancer false prediction

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Low-dose testosterone treatment decreases oxidative damage in TM3 Leydig cells

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Asian Journal of Andrology 2011年第3期13卷 432-437,512页

作者： Thomas IS Hwang Tien-Ling Liao ji-Fan Lin Yi-Chia Lin Shu-Yu Lee Yen-Chun Lai Shu-Huei kao Department of Urology Taipei Medical University Taipei Taiwan China Department of Urology School of Medicine Fu-Jen Catholic University Taipei Taiwan China Division of Urology Department of Surgery Shin Kong Wu Ho-Su Memorial Hospital Taipei Taiwan China School of Medical Laboratory Science and Biotechnology Taipei Medical University Taipei Taiwan China Central Laboratory Shin Kong Wu Ho-Su Memorial Hospital Taipei Taiwan China

Testosterone replacement therapy has benefits for aging men and those with hypogonadism. However, the effects of exogenous testosterone on Leydig cells are still unclear and need to be clarified. In this report, we demonstrate that testosterone supplementation can reduce oxidative damage in Leydig cells. The TM3 Leydig cell line was used as an in vitrocell model in this study. Cytoprotective effects were identified with 100-nmol l-1 testosterone treatment, but cytotoxic effects were found with ≥ 500-nmol l-1 testosterone supplementation. Significantly reduced reactive oxygen species （ROS） generation, lipid peroxide contents and hypoxia induction factor （HIF）-lα stabilization and activation were found with lO0-nmol 1-1 testosterone treatment. There was a 1.72-fold increase in ROS generation in the 500-nmol l-1 compared to the lO0-nmol l-1 testosterone treatment. A 1.58-fold increase in steroidogenic acute regulatory protein （STAR） expression was found in 50-nmol l-1 testosterone-treated cells （P〈0.01）. Chemically induced hypoxia was attenuated by testosterone supplementation. Leydig cells treated with low-dose testosterone supplementation showed cytoprotection by decreasing ROS and lipid peroxides, increasing StAR expression and relieving hypoxia stress as demonstrated by HIF-1α stabilization. Increased oxidative damage was found with ≥ 500-nmol l-1 testosterone manipulation. The mechanism governing the differential dose effects of testosterone on Leydig cells needs further investigation in order to shed light on testosterone replacement therapy.

关键词： Leydig cells oxidative damage reactive oxygen species testosterone

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HIV-1 Subtype Diversity and Factors Affecting Drug Resistance among Patients with Virologic Failure in Antiretroviral Therapy in Hainan Province,China,2014–2020

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生物医学与环境科学:英文版 2023年第9期36卷 800-813页

作者： YU De E XU Yu Jun LI Mu YANG Yuan LIANG Hua Yue ZHONG Shan Mei QIN Cai LAN Ya Nan LI Da Wei YU ji Peng PANG Yuan QIN Xue Qiu LIANG Hao ZHU kao kao YE Li LIANG Bing Yu Guangxi Key Laboratory of AIDS Prevention and Treatment School of Public HealthGuangxi Medical UniversityNanning 530021GuangxiChina International School of Public Health and One Health Hainan Medical UniversityHaikou 571199HainanChina Prevention and Treatment Department the Fifth People's Hospital of Hainan Province&Affiliated Dermatology Hospital of Hainan Medical UniversityHaikou 570102HainanChina Collaborative Innovation Centre of Regenerative Medicine and Medical BioResource Development and Application Co-constructed by the Province and Ministry Life Sciences InstituteGuangxi Medical UniversityNanning 530021GuangxiChina Oncology Medical College Guangxi Medical UniversityNanning 530021GuangxiChina The First Clinical Medical College Guangxi Medical UniversityNanning 530021GuangxiChinaGuangxi Key Laboratory of AIDS Prevention and TreatmentSchool of Public HealthGuangxi Medical UniversityNanning 530021GuangxiChina

Objective This study aimed to determine the HIV-1 subtype distribution and HIV drug resistance(HIVDR)in patients with ART failure from 2014 to 2020 in Hainan,*** A 7-year cross-sectional study was conducted among HIV/AIDS patients with ART failure in *** used online subtyping tools and the maximum likelihood phylogenetic tree to confirm the HIV subtypes with pol *** resistance mutations(DRMs)were analyzed using the Stanford University HIV Drug Resistance *** A total of 307 HIV-infected patients with ART failure were included,and 241 available pol sequences were *** 241 patients,CRF01_AE accounted for 68.88%,followed by CRF07_BC(17.00%)and eight other subtypes(14.12%).The overall prevalence of HIVDR was 61.41%,and the HIVDR against non-nucleoside reverse transcriptase inhibitors(NNRTIs),nucleotide reverse transcriptase inhibitors(NRTIs),and protease inhibitors(PIs)were 59.75%,45.64%,and 2.49%,*** patients,hypoimmunity or opportunistic infections in individuals,and samples from 2017 to 2020 increased the odd ratios of ***,HIVDR was less likely to affect female *** common DRMs to NNRTIs were K103N(21.99%)and Y181C(20.33%),and M184V(28.21%)and K65R(19.09%)were the main DRMs against *** The present study highlights the HIV-1 subtype diversity in Hainan and the importance of HIVDR surveillance over a long period.

关键词： HIV-1 subtypes Antiretroviral therapy Virological failure Drug resistance

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Robotic and laparoscopic hybrid pancreaticoduodenectomy： surgical techniques and early outcomes

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Chinese Medical Journal 2014年第16期127卷 3027-3029页

作者： ji Wu Ding Kai kao Xiaoming He Changsheng Li Ning Li jieshou Research Institute of General Surgery Jinling Hospital Nanjing University School of Medicine Nanjing Jiangsu 210002 China

In recent years, the use of minimally invasive surgery in pancreas has been gradually rising in importance. There has been growing interest in performing pancreatectomy by the laparoscopic approach. However, laparoscopic pancreaticoduodenectomy （LPD） is very complicated and difficult to control and requires surgeons to have a high level of laparoscopic skills. Since LPD provides no significant benefits in terms of blood loss, morbidity rate,

关键词： robotic surgery laparoscopic surgery pancreaticoduodenectomy

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共沉淀法合成Sm^(3+)掺杂的Sr_2CeO_4的荧光光谱

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光谱学与光谱分析 2005年第10期25卷 1739-1741页

作者：石士考栗俊敏周济河北师范大学化学学院河北石家庄050091 清华大学材料科学与工程系新型陶瓷和精细工艺国家重点实验室北京100084

以(NH4)2C2O4为沉淀剂,用Sr,Ce和Sm的硝酸盐为反应物,制备了Sr2CeO4:Sm的前驱体。将此前驱体烧结后,得到了Sm掺杂的Sr2CeO4白色荧光材料。其荧光光谱强度与烧结温度和Sm3+的掺杂浓度密切相关,当烧结温度为1 050℃以及Sm3+的掺杂浓度为1 ... 详细信息

以(NH4)2C2O4为沉淀剂,用Sr,Ce和Sm的硝酸盐为反应物,制备了Sr2CeO4:Sm的前驱体。将此前驱体烧结后,得到了Sm掺杂的Sr2CeO4白色荧光材料。其荧光光谱强度与烧结温度和Sm3+的掺杂浓度密切相关,当烧结温度为1 050℃以及Sm3+的掺杂浓度为1 mol%时,峰值为470 nm的宽带以及Sm3+在608和654 nm的线谱发射最强。同直接在高温固相反应下得到的样品进行比较,其发射光谱强度大大提高。

关键词：共沉淀法 Sr2CeO4 Sm^3+ 荧光光谱

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中国边缘海氮循环过程和源汇格局——以南海为例

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海洋与湖沼 2021年第2期52卷 314-322页

作者：杨进宇汤锦铭郭香会高树基厦门大学近海海洋环境科学国家重点实验室海洋与地球学院厦门361102

边缘海氮循环过程研究是全球海洋氮循环研究的重要组成部分,对全球氮源汇格局有显著影响,进而对全球气候变化产生反馈。人为活动和气候变化又是影响边缘海关键氮循环过程速率的重要因素。南海作为中国和西北太平洋最大的边缘海,是边缘... 详细信息

边缘海氮循环过程研究是全球海洋氮循环研究的重要组成部分,对全球氮源汇格局有显著影响,进而对全球气候变化产生反馈。人为活动和气候变化又是影响边缘海关键氮循环过程速率的重要因素。南海作为中国和西北太平洋最大的边缘海,是边缘海氮循环研究的理想场所。本文详细总结了南海近岸和海盆区的氮源汇过程及其内循环过程的最新研究进展,结果显示人为活动对上述过程的显著扰动。此外,全球变暖和海洋酸化正改变不同的氮循环过程速率,并可能引起南海氮收支平衡的不确定性。文章最后提出了边缘海氮循环研究的重要发展方向。

关键词：边缘海海洋氮循环源汇格局南海氮同位素

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KCl对含有纳米银的[Ru(bpy)_3]^(2+)荧光性能影响

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光谱学与光谱分析 2007年第7期27卷 1377-1380页

作者：王悦辉周济石士考清华大学新型陶瓷与精细工艺国家重点实验室北京100084

通过吸收光谱、荧光光谱等手段研究了KCl对含有纳米银的金属钌联吡啶配合物([Ru(bpy)3]2+)的荧光性能的影响。结果表明,KCl与纳米银粒子间存在较强的相互作用,这种强的相互作用破坏了[Ru(bpy)3]2+与纳米银粒子间的链状网络结构,形成大... 详细信息

通过吸收光谱、荧光光谱等手段研究了KCl对含有纳米银的金属钌联吡啶配合物([Ru(bpy)3]2+)的荧光性能的影响。结果表明,KCl与纳米银粒子间存在较强的相互作用,这种强的相互作用破坏了[Ru(bpy)3]2+与纳米银粒子间的链状网络结构,形成大颗粒和团聚体。同时,在吸收光谱的长波段处观察到明显的新的吸收带,随着KCl量的增加,新吸收带峰值逐渐红移并展宽。随着KCl量的增加,[Ru(bpy)3]2+荧光强度先猝灭而后逐渐增强,直至达到一个定值。文章从分子间的相互作用、能量传递等方面探讨了KCl对含有纳米银的[Ru(bpy)3]2+荧光性能影响机理。

关键词： KCl 纳米银 [Ru(bpy)3]^2+ 荧光增强荧光猝灭

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海洋氮循环中间体羟胺的研究进展

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地球科学进展 2023年第7期38卷 688-702页

作者：童森炜杨进宇万显会牛晴晴高树基厦门大学近海海洋环境科学国家重点实验室海洋与地球学院福建厦门361102 海南大学南海海洋资源利用国家重点实验室海洋学院海南海口570228

羟胺(NH_(2)OH)是海洋中极为活跃的痕量氮素之一,是氨氧化、硝酸盐异化还原成铵和厌氧氨氧化等诸多氮循环过程的关键中间产物,是构架海洋氮循环网络的重要组成。同时,NH_(2)OH也是温室气体氧化亚氮(N_(2)O)的重要前体物,与海洋N_(2)O的... 详细信息

羟胺(NH_(2)OH)是海洋中极为活跃的痕量氮素之一,是氨氧化、硝酸盐异化还原成铵和厌氧氨氧化等诸多氮循环过程的关键中间产物,是构架海洋氮循环网络的重要组成。同时,NH_(2)OH也是温室气体氧化亚氮(N_(2)O)的重要前体物,与海洋N_(2)O的产生与释放紧密关联。因此,系统理解NH_(2)OH在海洋中的源汇格局、时空变异及其调控机理,对刻画海洋氮循环以及气候效应至关重要。然而,由于NH_(2)OH在海洋中纳摩尔级别浓度及其复杂、活跃的迁移转化过程,使得海洋学界对于NH_(2)OH的认识仍不清晰。系统综述了当前关于海洋NH_(2)OH的研究进展,重点总结了NH_(2)OH潜在的源汇过程、测定方法及其对海洋N_(2)O产生的可能贡献,以及海洋中NH_(2)OH的分布特征及其潜在影响因素。最后,梳理了关于NH_(2)OH测定和影响其分布的可能机理等方面存在的问题和难点,提出未来海洋NH_(2)OH研究的建议与展望。

关键词：海洋氮循环羟胺氧化亚氮

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