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The CsMYB12. and CsbHLH111 are involved in drought stress‑induced anthocyanin biosynthesis in Chaenomeles speciosa

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Molecular Horticulture 2023年第1期3卷 51-63页

作者： Yanshen Ren shuangyu Zhang Qianyi Zhao Yang Wu Houhua Li College of Landscape Architecture and Art Northwest A&F UniversityYangling 712100ShaanxiChina

Drought stress has been demonstrated to enhance the biosynthesis of anthocyanins in the leaves,resulting in an increased aesthetic ***,the molecular mechanisms underlying drought-induced anthocyanin biosynthesis in Chaenomeles speciosa remain *** this study,the metabolites of *** leaves were analyzed,and it was found that the content of cyanidin-3-O-rutinoside increased significantly under drought *** differentially expressed genes CsMYB12. and CsbHLH111 were isolated by transcriptomics data analysis and gene cloning,and gene overexpression and VIGS experiments verified that both play important roles in anthocyanin ***,Y1H and Dual-luciferase reporter assay showed that CsMYB12. binds to the promoters of anthocyanin biosynthesis-related structural genes(such as CsCHI,CsF3H,and CsANS),while CsbHLH111 was shown to bind to the promoter of CsCHI,positively regulating its ***,BIFC and Y2. assays unveiled potential protein–protein interactions between CsMYB12. and CsbHLH111 at the cell ***,these results shed light on the critical roles played by CsMYB12. and CsbHLH111 in anthocyanin biosynthesis,thus providing a valuable insight into understanding the molecular mechanisms of how the MYB and bHLH genes regulate anthocyanin biosynthesis in the process of leaf coloration in ***.

关键词： Chaenomeles speciosa Drought CsbHLH111 CsMYB12. Anthocyanin

康复训练配合神经肌肉电刺激用于脑出血患者术后康复的效果

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中国实用医刊 2024年第11期51卷 53-56页

作者：李依婷程霜玉郝珺焦婧婧张津华开封市中心医院康复科开封475000

目的分析康复训练配合神经肌肉电刺激(NMES)用于脑出血患者术后康复的效果。方法队列研究。抽取2.2.年1月至2.2.年12.于开封市中心医院接受手术治疗的脑出血患者100例,根据随机数字表法分为对照组和观察组,每组50例。对照组采用常规康... 详细信息

目的分析康复训练配合神经肌肉电刺激(NMES)用于脑出血患者术后康复的效果。方法队列研究。抽取2.2.年1月至2.2.年12.于开封市中心医院接受手术治疗的脑出血患者100例,根据随机数字表法分为对照组和观察组,每组50例。对照组采用常规康复训练,观察组在对照组的基础上采用NMES治疗。比较两组康复效果、神经功能[美国国立卫生研究院卒中量表(NIHSS)评分]、运动功能[Fugl-Meye运动功能评分量表(FMA)评分]、日常生活能力[改良Barthel指数(MBI)、生活自理能力量表(ADL)评分]、凝血功能[纤维蛋白原(Fib)、D-二聚体(D-D)]。结果观察组总有效率(98.00%,49/50)高于对照组(84.00%,42.50),P<0.05。治疗后,观察组NIHSS评分低于对照组,且FMA评分高于对照组(P<0.05);观察组步速、步宽、步幅大于对照组(P<0.05);观察组Fib、D-D水平低于对照组(P<0.05)。结论康复训练配合NMES用于脑出血患者术后康复效果显著,能纠正血液高凝状况,可改善神经功能,提高生活质量。

关键词：脑出血康复训练神经肌肉电刺激术后康复

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基于淡以养脾理论从脾阴论治慢性萎缩性胃炎

新中医 2024年第18期56卷 194-198页

作者：陈必华陈双钰柴银英李佳扬裘生梁浙江中医药大学附属第一医院/浙江省中医院浙江杭州310006 浙江中医药大学第一临床医学院浙江杭州310053

通过查阅中医古籍与现代医学文献,从脾阴角度探析慢性萎缩性胃炎(CAG),并探微淡以养脾理论,且以之作为切入点详述治法方药,发现多种病因可造成脾阴虚,而脾阴虚作为病机在脾之运及化方面与CAG存在密切的关联。张锡纯提出的淡以养脾理论... 详细信息

通过查阅中医古籍与现代医学文献,从脾阴角度探析慢性萎缩性胃炎(CAG),并探微淡以养脾理论,且以之作为切入点详述治法方药,发现多种病因可造成脾阴虚,而脾阴虚作为病机在脾之运及化方面与CAG存在密切的关联。张锡纯提出的淡以养脾理论以淡入脾胃、阴虚专责重于脾为架构,并从阴阳五行、脏腑生理特性、解剖学、药性五味等方面阐释淡味补养脾阴的缘由及优势,从而引申出以淡为主,甘淡合用的治法及多种相应方药,治疗脾阴虚证有独到之处,为治疗脾阴虚型CAG拓展了思路,具有积极的临床意义。

关键词：慢性萎缩性胃炎脾阴虚淡以养脾张锡纯

Synergy between metallic components of MoNi alloy for catalyzing highly efficient hydrogen storage of MgH2

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Nano Research 2020年第8期13卷 2063-2071页

作者： Meng Chen Yanhui Pu Zhenyang Li Gang Huang Xiaofang Liu Yao Lu Wukui Tang Li Xu shuangyu Liu Ronghai Yu Jianglan Shui School of Materials Science and Engineering Beihang UniversityBeijing100191China Beijing Key Laboratory of Civil Aircraft Structures and Composite Material COMAC Beijing Aircraft Technology Research InstituteBeijing102211China Material Laboratory of State Grid Corporation of China Global Energy Interconnection Research Institute BeijingBeijing102211China

Catalysts play a critical role in improving the hydrogen storage kinetics in Mg/MgH2.*** highly efficient catalysts and catalyst design principles are hot topics but *** catalytic activity of metallic elements on dehydrogenation kinetics generally follows a sequence of Ti>Nb>Ni>V>Co>***,we report a highly efficient alloy catalyst composed of low-active elements of Mo and Ni(*** alloy)for MgH2.*** alloy nanoparticles show excellent catalytic effect,even outperforming most advanced Ti-based *** synergy between Mo and Ni elements can promote the break of Mg-H bonds and the dissociation of hydrogen molecules,thus significantly improves the kinetics of Mg/MgH2.*** MoNi-catalyzed Mg/MgH2.system can absorb and release 6.7 wt.%hydrogen within 60 s and 10 min at 300℃,respectively,and exhibits excellent cycling stability and low-temperature hydrogen storage *** study provides a strategy for designing efficient catalysts for hydrogen storage materials using the synergy of metal elements.

关键词： hydrogen storage Mg hydride catalyst synergetic effect MoNi alloy

小麦响应白粉菌侵染的表型特征、生理应答和免疫信号传导通路分析

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植物遗传资源学报 2024年第4期25卷 522-532页

作者：白双宇崔原瑗王昭懿何俊涛刘彩霞刘凤楼王掌军李清峰宁夏大学农学院银川750021

了解植物应对病原物入侵的反应对挖掘抗性基因具有重要意义。通过白粉菌侵染小麦中作9504幼苗,观察侵染后0 h、6 h、1 d、4 d和7 d小麦叶片生长、生理代谢相关指标和基因表达变化,揭示白粉菌侵染对小麦生长、渗透调节物质及活性氧的响... 详细信息

了解植物应对病原物入侵的反应对挖掘抗性基因具有重要意义。通过白粉菌侵染小麦中作9504幼苗,观察侵染后0 h、6 h、1 d、4 d和7 d小麦叶片生长、生理代谢相关指标和基因表达变化,揭示白粉菌侵染对小麦生长、渗透调节物质及活性氧的响应机制。结果表明,随着侵染时间的增加,小麦叶片死细胞数目、过氧化物酶活性和超氧阴离子数量呈上升趋势,白粉菌在7 d后生长出成熟的分生孢子。苯丙氨酸解氨酶和多酚氧化酶分别在4 d和1 d后发挥作用,可溶性蛋白含量随侵染时间增加基本呈上升趋势,叶绿素含量在7 d时显著下降,进而影响植物生长,过氧化氢含量在整个侵染时期变化不大。转录组分析发现侵染初期(0 h到6 h)PTI信号传导部分受抑制下调,侵染前中期(6 h到1 d和1 d到4 d)PTI信号传导积极响应,ETI出现在侵染前期,侵染后期(4 d到7 d)由于白粉菌已经完全定殖于叶片表面,影响小麦的光合作用,PTI和ETI信号传导通路均有下调的趋势。研究结果为进一步了解小麦响应白粉菌侵染的防御机制提供一定参考。

关键词：小麦白粉菌活性氧信号传导转录组分析

Effects of Foliage Boron-Spraying on Seed Yields and Fatty Acid Composition of Tree Peony(Paeonia ostii ‘Feng Dan’)

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Wuhan University Journal of Natural Sciences 2018年第1期23卷 56-62页

作者： LI Chengcheng JI Wenli SUN Jiayi LI Qiaoli YANG Jingxuan WEI shuangyu ZHANG Yanlong College of Landscape Architecture and Arts NorthwestAgriculture and Forestry University Yangling 712100 ShaanxiChina College of Forestry Northwest Agriculture and ForestryUniversity Yangling 712100 Shaanxi China

The influence of the foliage boron（B）-spaying concentration on the yield and fatty acid composition of tree peony（Paeonia ostii ＇Feng Dan＇） was explored in the experiment of 2.14-2.15. In this research, a statistically significant correlation was found between the mass concentration of foliage boron-spraying and tree peony seed yield. Maximum yield increment of tree peony seeds was obtained when the boron-spraying concentration was 4 g/L. The composition of fatty acid was analyzed by gas chromatography-mass spectrometry（GC-MS）, and the main components of the fatty acid composition were quantified by internal standard method. The increase in the oil extraction rate after foliage boron-spraying nutrition of different concentration was significant and ranged between 0.04% and 11.43%. Seed oil production and oil extraction rate were also increased due to the increase of seed yields. Furthermore, foliage boron-spraying had a significant effect on the content of linolenic acid and linoleic acid in seed oil.

关键词： tree peony foliage boron-spraying seed yield GC-MS fatty acid composition

硅烷偶联剂对氧化铝陶瓷浆料流动性能和3D打印性能的影响

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硅酸盐通报 2024年第3期43卷 1058-1069页

作者：缪新宇刘双宇陆萍张福隆 Vasilieva Tatiana Mikhailovna 黄传锦王立岩王斌华盐城工学院机械工程学院盐城224051 盐城工学院汽车工程学院盐城224051 莫斯科物理技术学院多尔戈普鲁德内141700 江苏铬莱伯数字科技有限公司盐城224001

基于光固化3D打印技术需要高固相含量、低黏度的陶瓷浆料以防止烧结陶瓷部件产生裂纹、孔洞、翘曲等缺陷,通过测试流变性能与固化性能,本文优化了树脂单体的选用及配比,采用KH550、KH560、KH570三种硅烷偶联剂对Al_(2.O_(3)粉体表面改性... 详细信息

基于光固化3D打印技术需要高固相含量、低黏度的陶瓷浆料以防止烧结陶瓷部件产生裂纹、孔洞、翘曲等缺陷,通过测试流变性能与固化性能,本文优化了树脂单体的选用及配比,采用KH550、KH560、KH570三种硅烷偶联剂对Al_(2.O_(3)粉体表面改性,以改善陶瓷浆料的流变性能和稳定性,探讨了硅烷偶联剂降低Al_(2.O_(3)陶瓷浆料体系黏度的机理,获得了固相含量为75%(质量分数)(体积分数为45.5%)、黏度为4540 mPa·s的Al_(2.O_(3)陶瓷浆料,并提出了一种光固化Al_(2.O_(3)陶瓷浆料制备的优化方法,这有望对用于制备复杂陶瓷的高固含量、低黏度的3D打印Al_(2.O_(3)陶瓷浆料提供帮助。

关键词：光固化技术氧化铝陶瓷浆料优化表面改性硅烷偶联剂 3D打印性能

Fe-N-C catalysts for PEMFC: Progress towards the commercial application under DOE reference

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Journal of Energy Chemistry 2019年第12期28卷 77-87页

作者： Lina Wang Xin Wan shuangyu Liu Li Xu Jianglan Shui School of Materials Science and Engineering Beihang UniversityBeijing 100083China Material Laboratory of State Grid Corporation of China Global Energy Interconnection Research InstituteBeijing 102211China

Proton exchange membrane fuel cells(PEMFC)have attracted much attention because of their high energy conversion efficiency,high power density and zero emission of ***,the high cost of the cathode platinum group metal(PGM)catalysts creates a barrier for the large-scale application of *** efforts have been devoted to the development of low-cost PGM-free catalysts,especially the Fe-N-C catalysts,to replace the expensive PGM ***,the characterization methods and evaluation standards of the catalysts varies,which is not conducive to the comparison of PGM-free *** of energy(DOE)is the only authority that specifies the testing standards and activity targets for PGM-free *** this review,the major breakthroughs of Fe-N-C catalysts are outlined with the reference of DOE standards and *** preparation and characteristics of these highly active Fe-N-C catalysts are briefly ***,the efforts on improving the mass transfer and the durability issue of Fe-N-C fuel cell are ***,the prospective directions concerning the comprehensive evaluation of the Fe-N-C catalysts are proposed.

关键词： PEMFC Fe-N-C catalysts U.S.DOE Test standards Activity targets

Arabidopsis RNA polymerase Ⅱ C-terminal domain phosphatase-like 1 targets mitogen-activated protein kinase cascades to suppress plant immunity

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Journal of Integrative Plant Biology 2023年第10期65卷 2380-2394页

作者： Junjun Wei Wei Sun Xinhang Zheng Shanshan Qiu shuangyu Jiao Kevin Babilonia Hisashi Koiwa Ping He Libo Shan Wenxian Sun Fuhao Cui Department of Plant Pathology and the Ministry of Agriculture Key Laboratory of Pest Monitoring and Green Management China Agricultural UniversityBeijing 100193China Department of Biochemistry and Biophysics Texas A&M UniversityCollege StationTX 77843USA Department of Horticultural Sciences Texas A&M UniversityCollege StationTX 77843USA

Mitogen-activated protein kinase(MAPK) cascades play pivotal roles in plant defense against phytopathogens downstream of immune receptor complexes. The amplitude and duration of MAPK activation must be strictly controlled, but the underlying mechanism remains unclear. Here, we identified Arabidopsis CPL1(C-terminal domain phosphatase-like 1)as a negative regulator of microbe-associated molecular pattern(MAMP)-triggered immunity via a forward-genetic screen. Disruption of CPL1 significantly enhanced plant resistance to Pseudomonas pathogens induced by the bacterial peptide fg2.. Furthermore, fg2.-induced MPK3/MPK4/MPK6 phosphorylation was dramatically elevated in cpl1 mutants but severely impaired in CPL1 overexpression lines, suggesting that CPL1 might interfere with fg2.-induced MAPK activation. Indeed, CPL1 directly interacted with MPK3 and MPK6, as well as the upstream MKK4 and MKK5. A firefy luciferase-based complementation assay indicated that the interaction between MKK4/MKK5 and MPK3/MPK6 was significantly reduced in the presence of CPL1. These results suggest that CPL1 plays a novel regulatory role in suppressing MAMP-induced MAPK cascade activation and MAMP-triggered immunity to bacterial pathogens.

关键词： Arabidopsis C-terminal domain(CTD)phosphatase-like 1(CPL1) microbe-associated molecular pattern(MAMP) mitogen-activated protein kinase(MAPK) pattern-triggered immunity(PTI)