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Diagnose and treatment for Type D congenital esophageal atresia with tracheoesophageal fistula

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儿科学研究(英文) 2024年第1期8卷 37-43页

作者： Wang Dingding Zhao Yong Zhang Yanan Hua Kaiyun Gu Yichao li shuangshuang liao Junmin Yang Shen Yang Ting Zhao Jiawei Huang Jinshi Department of Neonatal Surgery Beijing Children’s Hospital Capital Medical University National Center for Children’s Health Beijing China

Importance: Type D esophageal atresia (EA) with tracheoesophageal fistula (TEF) is characterized by EA with both proximal and distal TEFs. It is a rare congenital anomaly with a very low incidence.Objective: To investigate diagnostic and treatment strategies for this rare condition.Methods: We retrospectively reviewed the clinicopathological features of patients with EA/TEF treated at our institution between January 2007 and September 2021.Results: Among 386 patients with EA/TEF, 14 (3.6%) had type D EA/TEF. Only two patients were diagnosed with proximal TEF preoperatively. Seven patients were diagnosed intraoperatively. Five patients were missed for diagnosis during the initial surgery but was later confirmed by bronchoscopy. During the neonatal period, seven patients underwent a one-stage repair of proximal and distal TEF via thoracoscopy or thoracotomy. Due to missed diagnosis and other reasons, the other 7 patients underwent two-stage surgery for repair of the proximal TEF, including cervical incision and thoracoscopy. Ten of the 14 patients experienced postoperative complications including anastomotic leakage, pneumothorax, esophageal stricture, and recurrence. Patients who underwent one-stage repair of distal and proximal TEF during the neonatal period showed a higher incidence of anastomotic leak (4/7). In contrast, only one of seven patients with two-stage repair of the proximal TEF developed an anastomotic leak.Interpretation: Type D EA/TEF is a rare condition, and proximal TEFs are easily missed. Bronchoscopy may aim to diagnose and determine the correct surgical approach. A cervical approach may be more suitable for repairing the proximal TEF.

关键词： Diagnosis Therapy Tracheoesophageal fistula Type D congenital esophageal atresia

Establishment of a humanized ST6GAL1 mouse model for influenza research

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Animal Models and Experimental Medicine 2024年第3期7卷 337-346页

作者： Lyu Chao Han Feng Gao Qian Lv limin Lu Ziwei Lu shuangshuang li Xiaoyan Hu Yuechao Yang Mengjie Zhao Yingze liu Jun Lu Xuancheng Duo Shuguang National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases Chinese Center for Disease Control and PreventionBeijingChina Institute of Zoology Chinese Academy of SciencesBeijingChina NHC Key Laboratory of Biosafety National Key Laboratory of Intelligent Tracking and Forecasting for Infectious DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijingChina Zhejiang Provincial Key Laboratory of Medical Genetics Key Laboratory of Laboratory MedicineMinistry of EducationSchool of Laboratory Medicine and Life SciencesWenzhou Medical UniversityWenzhouChina

Background:This study aimed to construct and characterize a humanized influenza mouse model expressing hST6GAL1.Methods:Humanized fragments,consisting of the endothelial cell-specific K18 promoter,human ST6GAL1-encoding gene,and luciferase gene,were microinjected into the fertilized eggs of mice.The manipulated embryos were transferred into the oviducts of pseudopregnant female mice.The offspring were identified using PCR.Mice exhibiting elevated expression of the hST6GAL1 gene were selectively bred for propagation,and in vivo analysis was performed for screening.Expression of the humanized gene was tested by performing immunohistochemical(IHC)analysis.Hematologic and biochemical analyses using the whole blood and serum of humanized hST6GAL1 mice were performed.Results:Successful integration of the human ST6GAL1 gene into the mouse genome led to the overexpression of human SiaT ST6GAL1.Seven mice were identified as carrying copies of the humanized gene,and the in vivo analysis indicated that hST6GAL1gene expression in positive mice mirrored influenza virus infection characteristics.The IHC results revealed that hST6GAL1 was expressed in the lungs of humanized mice.Moreover,the hematologic and biochemical parameters of the positive mice were within the normal range.Conclusion:A humanized influenza mouse model expressing the hST6GAL1 gene was successfully established and characterized.

关键词： hST6GAL1 humanized mice influenza animal model

A surface-eroding poly(1,3-trimethylene carbonate)coating for magnesium based cardiovascular stents with stable drug release and improved corrosion resistance

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Bioactive Materials 2022年第1期7卷 144-153页

作者： Hongyan Tang shuangshuang li Yuan Zhao Cunli liu Xuenan Gu Yubo Fan Key Laboratory for Biomechanics and Mechanobiology of Ministry of Education School of Biological Science and Medical EngineeringBeihang UniversityBeijing10083China Beijing Advanced Innovation Centre for Biomedical Engineering Beihang UniversityBeijing102402China

Magnesium alloys with integration of degradability and good mechanical performance are desired for vascular stent application.Drug-eluting coatings may optimize the corrosion profiles of magnesium substrate and reduce the incidence of restenosis simultaneously.In this paper,poly(trimethylene carbonate)(PTMC)with different molecular weight(50,000 g/mol named as PTMC5 and 350,000 g/mol named as PTMC35)was applied as drug-eluting coatings on magnesium alloys.A conventional antiproliferative drug,paclitaxel(PTX),was incorporated in the PTMC coating.The adhesive strength,corrosion behavior,drug release and biocompatibility were investigated.Compared with the PLGA control group,PTMC coating was uniform and gradually degraded from surface to inside,which could provide long-term protection for the magnesium substrate.PTMC35 coated samples exhibited much slower corrosion rate 0.05μA/cm^(2)in comparison with 0.11μA/cm^(2)and 0.13μA/cm^(2)for PLGA and PTMC5 coated counterparts.In addition,PTMC35 coating showed more stable and sustained drug release ability and effectively inhibited the proliferation of human umbilical vein vascular smooth muscle cells.Hemocompatibility test indicated that few platelets were adhered on PTMC5 and PTMC35 coatings.PTMC35 coating,exhibiting surface erosion behavior,stable drug release and good biocompatibility,could be a good candidate as a drug-eluting coating for magnesium-based stent.

关键词： Magnesium alloy Polymeric coating Surface erosion Drug release Cardiovascular stents

Trace ruthenium promoted dual-reconstruction of CoFeP@C/NF for activating overall water splitting performance beyond precious-metals

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Nano Research 2023年第8期16卷 10810-10821页

作者： Jinhong Ren Jie liu Yunmei Du shuangshuang li Mengmeng Wang Bin li Bo Yang Lei Wang Yanru liu College of Environment and Safety Engineering Qingdao University of Science and TechnologyQingdao 266042China College of Chemistry and Molecular Engineering Qingdao University of Science and TechnologyKey Laboratory of Eco-chemical EngineeringKey Laboratory of Optic-electric Sensing and Analytical Chemistry of Life ScienceTaishan Scholar Advantage and Characteristic Discipline Team of Eco Chemical Process and TechnologyQingdao 266042China College of Materials Science and Engineering Qingdao University of Science and TechnologyQingdao 266042China

At present,Ru dopants mainly enhance electrocatalytic performance by inducing strain,vacancy,local electron difference,and synergy.Surprisingly,this work innovatively proposes that trace Ru atoms induce dual-reconstruction of phosphide by regulating the electronic configuration and proportion of Co–P/Co–O species,and ultimately activate superb electrocatalytic performance.Specifically,Ru-CoFeP@C/nickel foam(NF)is reconstructed to generate hydrophilic Co(OH)_(2)nanosheets during the hydrogen evolution reaction(HER)process,further accelerating the alkaline HER kinetics of phosphide.And the as-formed CoOOH during the oxygen evolution reaction(OER)process directly accelerates the oxygen overflow efficiency.As expected,the overpotential at 100 mA·cm^(−2)(η100)values of the reconstructed Ru-CoFeP@C/NF are 0.104 and 0.257 V for HER and OER,which are greatly lower than that of Pt/C-NF and RuO_(2)-NF benchmarks,respectively.This work provides guidance for the construction of highperformance catalysts for HER and OER dual reconstruction.This work provides a new idea for the optimization of catalyst structure and electrocatalytic performance.

关键词： Ru doping dual reconstruction overall water splitting phosphide Ru-CoFeP@C/nickel foam(NF)

Ultralight and ordered lamellar polyimide-based graphene foams with efficient broadband electromagnetic absorption

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Journal of Materials Science & Technology 2022年第7期102卷 97-104页

作者： Yawei Zhang shuangshuang li Xinwei Tang Wei Fan Qianqian Lan Le li Piming Ma Weifu Dong Zicheng Wang Tianxi liu The Key Laboratory of Synthetic and Biological Colloids Ministry of EducationInstitute of Nanocomposites and Energy MaterialsSchool of Chemical and Material EngineeringJiangnan UniversityWuxi 214122China State Key Laboratory for Modification of Chemical Fibers and Polymer Materials College of Materials Science and EngineeringDonghua UniversityShanghai 201620China

The development of high-performance microwave absorption materials with strong absorption capacity and broad bandwidth is highly desirable in the field of electromagnetic pollution protection.Herein,ultralight polyimide-based graphene foam with ordered lamellar structure is precisely designed and controllably constructed by bidirectional freezing process.More lamellar interfaces formed inside the foam per unit volume effectively facilitate the layer-by-layer dissipation for the vertical incident electromagnetic waves,thereby endowing the foam with efficient broadband electromagnetic absorption performance.More importantly,electromagnetic absorption performance can be controllably adjusted by optimizing impedance distribution and microstructure of skeletons.As a result,the optimized foam with an ultralow density of 9.10 mg/cm^(3)presents a minimum reflection loss value of-61.29 dB at 9.25 GHz and an effective absorption bandwidth of 5.51 GHz(7.06-12.57 GHz,covering the whole X band) when the thickness is 4.75 mm.

关键词： Polyimide Graphene Foam Ordered lamellar structure Microwave absorption

Effects of combined soluble gas stabilization， modified atmosphere packaging and cold plasma on controlling chilled chicken breasts spoilage

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Food Science and Human Wellness 2024年

作者： Jing li Chen Xu Yifeng Lu Xiaoming Wang shuangshuang Jin Yun Bai Xia Wang Xinglian Xu Minyi Han Key Laboratory of Meat Processing and Quality Control Ministry of Education Jiangsu Synergetic Innovation Center of Meat Production and Processing and College of Food Science and Technology Nanjing Agricultural University Wens Foodstuff Group Co. Ltd.

The hurdle technology for food preservation effectively addresses the limitations of individual antimicrobial technologies by integrating their strengths. It can not only prolong the storage time of food but also maintains its high quality. In this study， three antimicrobial and bactericidal technologies， namely soluble gas stabilization (SGS)， modified atmosphere packaging (MAP)， and cold plasma (CP)， were applied to chilled chicken breasts. The packaging， total viable count， and physicochemical properties of chilled chicken breasts after treatments and storage at 4℃ were monitored. The microbial diversity at the initial and end points of the storage time of each group was also analyzed. The results indicated that a (3-5) h SGS treatment can effectively increase the proportion of carbon dioxide in the MAP during the storage process of chilled chicken breasts， thereby alleviating the packaging collapse problem. Simultaneously， the effect of SGS， MAP， and CP combinational treatments significantly extended the storage time of chilled chicken breasts while maintaining the physicochemical qualities of samples. Compared to the control group， the total viable count (TVC) of chicken breast treated with SGS， MAP， and CP treatments decreased by 0.58 log CFU/g at 0 d. The shelf life was extended by 5 days. After 8 d， the total volatile basic nitrogen (TVB-N) was 26.67 vs 19.50 mg/100g， thiobarbituric acid reactive substances (TBARS) was 0.99 vs 0.72 mg MDA/kg， and TVC was 8.22 vs 6.52 log CFU/g. High-throughput sequencing results showed that SGS and MAP treatments significantly reduce the proportion of Pseudomonas and Psychrobacter， which are sensitive to carbon dioxide， in the total bacterial genera. This study underscores the potential of integrating multiple antimicrobial technologies for effective food preservation.

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Clinical characteristics and prognosis of 69 cases of neonatal appendicitis

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Pediatric Investigation 2023年第2期7卷 95-101页

作者： Yong Zhao Caihan Tang Jinshi Huang Junmin liao Yichao Gu Kaiyun Hua Yanan Zhang Yongwei Chen shuangshuang li Department of Neonatal Surgery Beijing Children’s HospitalCapital Medical UniversityNational Center for Children’s HealthBeijingChina

Importance:Neonatal appendicitis(NA)is a rare and potentially fatal neonatal disease.However,misdiagnosis is common owing to atypical clinical manifestations and non-specific laboratory tests.Objective:The aim of this study was to summarize the clinical characteristics,treatments,and prognoses of infants with NA.Methods:This retrospective analysis included 69 patients diagnosed with NA admitted to Beijing Children’s Hospital between 1980 and 2019.The patients were divided into surgical and non-surgical groups based on whether surgery was performed.Their clinical characteristics were analyzed using the chi-square test,t-test,or the Mann-Whitney U test.Results:The study included 47 males and 22 females with NA.The primary symptoms were abdominal distension(n=36,52.2%),fever(n=19,27.5%),refusal to feed or decreased feeding(n=16,23.2%),and vomiting(n=15,21.7%).Sixty-five patients underwent abdominal ultrasound examinations;43 had definite appendiceal abnormalities,10 had right lower abdominal adhesive masses,and 14 had neonatal enterocolitis manifestations.Twenty-nine and 40 patients were in the surgical and non-surgical groups,respectively.No statistically significant differences were observed between the groups regarding sex,age at onset,birth weight,admission weight,or hospitalization time.However,parenteral nutrition was prolonged in the surgical group(P=0.001).Additionally,two patients(2.9%)died.Interpretation:NA is a rare neonatal disease with atypical clinical manifestations.Abdominal ultrasonography may aid in the diagnosis.Similarly,appropriate treatment can improve the prognosis.

关键词： Acute appendicitis Diagnosis Neonatal Therapy

Genomic Epidemiology of Carbapenemaseproducing Klebsiella pneumoniae in China

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1154-1167页

作者： Cuidan li Xiaoyuan Jiang Tingting Yang Yingjiao Ju Zhe Yin liya Yue Guannan Ma Xuebing Wang Ying Jing Xinhua Luo shuangshuang li Xue Yang Fei Chen Dongsheng Zhou CAS Key Laboratory of Genome Sciences&Information Beijing Institute of GenomicsChinese Academy of Sciences and China National Center for BioinformationBeijing 100101China State Key Laboratory of Pathogen and Biosecurity Beijing Institute of Microbiology and EpidemiologyBeijing 100071China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Pathogenesis Prevention and Treatment of High Incidence Diseases in Central AsiaUrumqi 830011China Beijing Key Laboratory of Genome and Precision Medicine Technologies Beijing 100101China

The rapid spread of carbapenemase-producing Klebsiella pneumoniae(cpKP)poses serious threats to public health;however,the underlying genetic basis for its dissemination is still unknown.We conducted a comprehensive genomic epidemiology analysis on 420 cpKP isolates collected from 70 hospitals in 24 provinces/autonomous regions/municipalities of China during 2009–2017 by short-/long-read sequencing.The results showed that most cpKP isolates were categorized into clonal group 258(CG258),in which ST11 was the dominant clone.Phylogenetic analysis revealed three major clades including the top one of Clade 3 for CG258 cpKP isolates.Additionally,carbapenemase gene analysis indicated that blaKPC was dominant in the cpKP isolates,and most blaKPC genes were located in five major incompatibility(Inc)groups of blaKPC-harboring plasmids.Importantly,three advantageous combinations of host–blaKPC-carrying plasmid(Clade 3.1+3.2–IncFIIpHN7A8,Clade 3.1+3.2–IncFIIpHN7A8:IncR,and Clade 3.3–IncFIIpHN7A8:IncpA1763-KPC)were identified to confer cpKP isolates the advantages in both genotypes(strong correlation/coevolution)and phenotypes(resistance/growth/competition)to facilitate the nationwide spread of ST11/CG258 cpKP.Intriguingly,Bayesian skyline analysis illustrated that the three advantageous combinations might be directly associated with the strong population expansion during 2007–2008 and subsequent maintenance of the population of ST11/CG258 cpKP after 2008.We then examined drug resistance profiles of these cpKP isolates and proposed combination treatment regimens for CG258/non-CG258 cpKP infections.Thus,the findings of our systematical analysis shed light on the molecular epidemiology and genetic basis for the dissemination of ST11/CG258 cpKP in China,and much emphasis should be given to the close monitoring of advantageous cpKP–plasmid combinations.

关键词： Klebsiella pneumoniae Drug resistance Carbapenemase Plasmid Genomic epidemiology

Submerged vegetation removal promotes shift of dominant phytoplankton functional groups in a eutrophic lake

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Journal of Environmental Sciences 2014年第8期26卷 1699-1707页

作者： Jing Dong Kai Yang shuangshuang li Genbao li lirong Song State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of Sciences University of Chinese Academy of Sciences Research Center for Eco-Environmental Sciences Chinese Academy of Sciences

Historical data indicate that the dominance of submerged plants in Dianchi Lake in the 1960 s was characterized by low algal density with dominance of non-toxic group J（Scenedesmus,Pediastrum,etc.）. The removal of submerged plants,which began in the 1970 s,resulted in the expansion of bloom-forming Microcystis（group M）. Laboratory experiments suggested that Microcystis aeruginosa was inclined to grow and develop at elevated temperatures. The growth of Scenedesmus obliquus was slower than that of co-cultivated M. aeruginosa in the absence of Ceratophyllum demersum,especially at higher temperatures. The existence of submerged plant C. demersum could inhibit the growth of the harmful algae M. aeruginosa and this inhibitory effect by C. demersum was enhanced with an increase in temperature. Instead,with C. demersum,the growth of S. obliquus was not inhibited,but the co-cultivated M. aeruginosa was eliminated in a short time. Combined with the historical data and laboratory experiments,it was indicated that the submerged plants might play important roles in the dominance of the non-toxic group J in the historical succession. Consequently,the introduction of the submerged plant such as C. demersum might alter the dominant phytoplankton functional groups from M to J and benefit the restoration of the eutrophic lake.

关键词： Submerged vegetation,Phytoplankton,Functional groups