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Journal of Integrative Plant Biology 2024年第3期66卷 468-483页

作者： shuanghe cao Bingyan Liu Daowen Wang Awais Rasheed Lina Xie Xianchun Xia Zhonghu He State Key Laboratory of Crop Gene Resources and Breeding/National Wheat Improvement Center Institute of Crop SciencesBeijing 100081China College of Agronomy Henan Agricultural UniversityZhengzhou 450002China International Maize and Wheat Improvement Center(CIMMYT)China Office Chinese Academy of Agricultural SciencesBeijing 100081China

Achieving high yield and good quality in crops is essential for human food security and ***,there is usually disharmony between yield and *** storage protein(SSP)and starch,the predominant components in cereal grains,determine yield and quality,and their coupled synthesis causes a yield–quality ***,dissection of the underlying regulatory mechanism facilitates simultaneous improvement of yield and ***,we summarize current findings about the synergistic molecular machinery underpinning SSP and starch synthesis in the leading staple cereal crops,including maize,rice and *** further evaluate the functional conservation and differentiation of key regulators and specify feasible research approaches to identify additional regulators and expand *** also present major strategies to leverage resultant information for simultaneous improvement of yield and quality by molecular ***,future perspectives on major challenges are proposed.

关键词： cereal crops grain yield and quality seed storage protein starch synergistic regulation

Dissecting conserved cis-regulatory modules of Glu-1 promoters which confer the highly active endosperm-specific expression via stable wheat transformation

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The Crop Journal 2019年第1期7卷 8-18页

作者： Jihu Li Ke Wang Genying Li Yulian Li Yong Zhang Zhiyong Liu Xingguo Ye Xianchun Xia Zhonghu He shuanghe cao National Wheat Improvement Center Institute of Crop Sciences Chinese Academy of Agricultural Sciences College of Agronomy and Biotechnology China Agricultural University Crop Research Institute Shandong Academy of Agricultural Sciences International Maize and Wheat Improvement Center (CIMMYT) China Office c/o CAAS

Wheat high-molecular-weight glutenin subunits(HMW-GS) determine dough elasticity and play an essential role in processing quality. HMW-GS are encoded by Glu-1 genes and controlled primarily at transcriptional level, implemented through the interactions between cis-acting elements and trans-acting factors. However, transcriptional mechanism of Glu-1 genes remains elusive. Here we made a comprehensive analysis of cis-regulatory elements within 1-kb upstream of the Glu-1 start codon(-1000 to-1) and identified 30 conserved motifs. Based on motif distribution pattern, three conserved cis-regulatory modules(CCRMs), CCRM1(-300 to-101), CCRM2(-650 to-400), and CCRM3(-950 to-750), were defined, and their functions were characterized in wheat stable transgenic lines transformed with progressive 5′ deletion promoter::GUS fusion constructs. GUS staining, qP CR and enzyme activity assays indicated that CCRM2 and CCRM3 could enhance the expression level of Glu-1, whereas the 300-bp promoter(-300 to-1), spanning CCRM1 and core region(-100 to-1), was enough to ensure accurate Glu-1 initiation at 7 days after flowering(DAF) and shape its spatiotemporal expression pattern during seed development. Further transgenic assays demonstrated that CCRM1-2(-300 to-209) containing Complete HMW Enhancer(-246 to-209) was important for expression level but had no effect on expression specificity in the endosperm. In contrast, CCRM1-1(-208 to-101) was critical for both expression specificity and level of Glu-1. Our findings not only provide new insights to uncover Glu-1 transcription regulatory machinery but also lay foundations for modifying Glu-1 expression.

关键词： Conserved cis-regulatory modules Glu-1 Transcriptional regulation Transgenic wheat Triticum aestivum

QTL mapping for pre-harvest sprouting in a recombinant inbred line population of elite wheat varieties Zhongmai 578 and Jimai 22

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The Crop Journal 2023年第3期11卷 863-869页

作者： Rabiu Sani Shawai Dan Liu Lingli Li Tiantian Chen Ming Li shuanghe cao Xianchun Xia Jindong Liu Zhonghu He Yong Zhang Institute of Crop Sciences National Wheat Improvement CenterChinese Academy of Agricultural SciencesBeijing 100081China Department of Crop Science Faculty of Agriculture and Agricultural TechnologyKano University of Science and Technology WudilKano 713281Nigeria International Maize and Wheat Improvement Center(CIMMYT) China Officec/o CAASBeijing 100081China

Pre-harvest sprouting(PHS)is one of the serious global issues in wheat *** of quantitative trait loci(QTL)and closely-linked markers is greatly helpful for wheat *** the present study,a recombinant inbred line(RIL)population derived from the cross of Zhongmai 578(ZM578)/Jimai 22(JM22)and parents were phenotyped in five environments and genotyped by the wheat 50 K single-nucleotide polymorphism(SNP)*** QTL of germination index(GI),***-3A and ***-5A,were detected,explaining 4.33%–5.58%and 4.43%–8.02%of the phenotypic variances,*** resistant effect of ***-3A was contributed by JM22,whereas that of ***.5A was from *** two QTL did not correspond to any previously identified genes or genetic loci for PHSrelated traits according to their locations in the Chinese Spring reference genome,indicating that they are likely to be new loci for PHS *** kompetitive allele-specific PCR(KASP)markers K_AX-109605367and K_AX-179559687 flanking ***-3A,and K_AX-111258240 and K_AX-109402944flanking ***-5A,were developed and validated in a natural population of 100 wheat *** distribution frequency of resistance alleles at ***-3A and ***-5A loci were 82.7%and57.1%,respectively,in the natural *** findings provide new QTL and tightly linked KASP markers for improvement of PHS resistance in wheat.

关键词： Pre-harvest sprouting Germination index Quantitative trait loci KASP marker Triticum aestivum L.

Identification and validation of stable quantitative trait loci for yield component traits in wheat

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The Crop Journal 2023年第2期11卷 558-563页

作者： Lingli Li Yingjie Bian Yan Dong Jie Song Dan Liu Jianqi Zeng Fengju Wang Yong Zhang Zhonghu He Xianchun Xia Yan Zhang shuanghe cao Institute of Crop Sciences National Wheat Improvement CenterChinese Academy of Agricultural SciencesBeijing 100081China International Maize and Wheat Improvement Center(CIMMYT)China Office Beijing 100081China

Grain weight and grain number are important yield component traits in wheat and identification of underlying genetic loci is helpful for improving ***,we identified eight stable quantitative trait loci(QTL)for yield component traits,including five loci for thousand grain weight(TGW)and three for grain number per spike(GNS)in a recombinant inbred line population derived from cross Yangxiaomai/Zhongyou 9507 across four *** grain size is a major determinant of grain weight,we also mapped QTL for grain length(GL)and grain width(GW).***-2D,***-3B,***-5A and ***-7A.2 for TGW co-located with those for grain ***-2D also had a consistent genetic position with ***-2D,suggesting that the pleiotropic locus is a modulator of trade-off effect between TGW and *** and linkage mapping showed that TaGL3-5A and WAPO-A1 were candidate genes of ***-5A and ***-7A.2,*** developed Kompetitive allele specific PCR(KASP)markers linked with the stable QTL for yield component traits and validated their genetic effects in a diverse panel of wheat cultivars from the Huang-Huai River Valley ***-based genotyping analysis further revealed that the superior alleles of all stable QTL for TGW but not GNS were subject to positive selection,indicating that yield improvement in the region largely depends on increased *** analyses with previous studies showed that most of the QTL could be detected in different genetic backgrounds,and ***-7A.1 is likely a new *** findings provide not only valuable genetic information for yield improvement but also useful tools for marker-assisted selection.

关键词： Grain number per spike KASP marker QTL mapping Thousand grain weight Triticum aestivum

In silico curation of QTL-rich clusters and candidate gene identification for plant height of bread wheat

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作物学报:英文版 2023年第5期11卷 1480-1490页

作者： Dengan Xu Chenfei Jia Xinru Lyu Tingzhi Yang Huimin Qin Yalin Wang Qianlin Hao Wenxing Liu Xuehuan Dai Jianbin Zeng Hongsheng Zhang Xianchun Xia Zhonghu He shuanghe cao Wujun Ma College of Agronomy Qingdao Agricultural UniversityQingdao 266109ShandongChina National Wheat Improvement Center Institute of Crop ScienceChinese Academy of Agricultural SciencesBeijing 100081China

Many genetic loci for wheat plant height(PH) have been reported, and 26 dwarfing genes have been catalogued. To identify major and stable genetic loci for PH, here we thoroughly summarized these functionally or genetic verified dwarfing loci from QTL linkage analysis and genome-wide association study published from 2003 to 2022. A total of 332 QTL, 270 GWAS loci and 83 genes for PH were integrated onto chromosomes according to their locations in the IWGSC RefSeq v2.1 and 65 QTL-rich clusters(QRC) were defined. Candidate genes in each QRC were predicted based on IWGSC Annotation v2.1 and the information on functional validation of homologous genes in other species. A total of 38 candidate genes were predicted for 65 QRC including three GA2ox genes in QRC-4B-IV, QRC-5A-VIII and QRC-6A-II(Rht24) as well as GA 20-oxidase 2(TaSD1-3A) in QRC-3A-IV. These outcomes lay concrete foundations for mapbased cloning of wheat dwarfing genes and application in breeding.

关键词： QTL-rich clusters Plant height Semi-dwarfism Reduced height genes Candidate genes

Isolation and Characterization of Novel Variants of HMW Glutenin Subunits Genes from the St Genome of Pseudoroegneria Stipifolia and Elytrigia Stipifolia

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Isolation and Characterization of Novel Variants of HMW Glut...

2010全国植物生物学研讨会

作者： Li Zhixin cao shuanghe Zhang Xiangqi Zhang Huaigang Institute of Crop Genetics and Breeding Yangtze University Northwest Institute of Plateau Biology Chinese Academy of Sciences Institute of Genetics and Developmental Biology Chinese Academy of Sciences

The x-and y-type high molecular weight(HMW)glutenin subunits are conserved seed storage proteins in wheat and related *** we characterized the HMW glutenin subunits from Pseudoroegneria stipifolia(St St genomes)and El... 详细信息

关键词： HMW glutenin subunit Pseudoroegneria stipifolia Elytrigia stipifolia phylogenetic analysis evolution

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贵州导线覆冰的类型与分布特征

南方电网技术 2015年第4期9卷 84-89页

作者：彭赤罗洪黄欢曹双和陈百炼贵州电网有限责任公司贵阳550002 贵州电力试验研究院贵阳550003 贵州电力设计研究院贵阳550001 贵州山地环境气候研究所贵阳550002

综合利用气象站导线积冰观测资料和电力部门线路覆冰调查资料,全面分析了贵州导线覆冰的类型和分布特征。贵州导线覆冰类型包括雨凇、雾凇、湿雪和多种混合冻结类型,混合冻结类型比例高于单一类型覆冰;雨凇和湿雪的混合冻结雪凝在全省... 详细信息

综合利用气象站导线积冰观测资料和电力部门线路覆冰调查资料,全面分析了贵州导线覆冰的类型和分布特征。贵州导线覆冰类型包括雨凇、雾凇、湿雪和多种混合冻结类型,混合冻结类型比例高于单一类型覆冰;雨凇和湿雪的混合冻结雪凝在全省较为普遍,出现比例不亚于雨凇;贵州冰冻灾害多年常态分布总体上呈西部多东部少、中部多南北少的特征,但各地覆冰类型随海拔高度与地形环境变化较大;由于贵州中、东部地区覆冰以危害极大的雨凇、雪凝为主,故极端情况下覆冰强度要大于西部冰冻高发区,这也是2008年特大冰灾中东部地区受灾更重的原因。

关键词：导线覆冰覆冰类型分布特征

MIKESHE在电厂洪峰计算中的应用与结果分析

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人民长江 2015年第9期46卷 26-30页

作者：汤旻曹双和胡红兵任曦贵州电力设计研究院贵州贵阳550002

传统的水电厂、变电站设计洪水计算方法都是以经验公式或推理公式为基础建立的简单计算法,计算结果的正确率都较低。采用MIKE SHE分布式水文模型,结合GIS与RS技术,对贵州喀斯特地貌区的六硐河甲茶水电站进行了洪峰流量计算,并与其他模... 详细信息

传统的水电厂、变电站设计洪水计算方法都是以经验公式或推理公式为基础建立的简单计算法,计算结果的正确率都较低。采用MIKE SHE分布式水文模型,结合GIS与RS技术,对贵州喀斯特地貌区的六硐河甲茶水电站进行了洪峰流量计算,并与其他模型方法的计算结果进行了比较。结果表明,在洪峰计算中,MIKE SHE模型具有模拟结果更加精确、详细等独特优势,且适用于喀斯特地貌区。该模型的成功使用为今后类似地区的洪峰计算奠定了坚实的基础。

关键词： MIKE SHE模型分布式水文模型洪峰计算甲茶水电站贵州省

维普期刊数据库博看期刊

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贵州冬季电线积冰及其天气成因分析

气象 2014年第3期40卷 355-363页

作者：陈百炼吴战平张艳梅曹双和贵州省山地环境气候研究所贵阳550002 贵州山地气候与资源重点实验室贵阳550002 贵州省气候中心贵阳550002 贵州省六盘水市气象局六盘水553001 贵州省电力设计研究院贵阳550002

利用气象部门长期电线积冰观测和电力部门线路覆冰调查资料,对贵州冬季电线积冰的分布、类型特征与气象条件进行了综合分析,着重探讨了贵州电线积冰的形成机理和天气成因,主要结论:(1)贵州电线积冰的类型复杂多样,除具有雨凇、雾凇、雪... 详细信息

利用气象部门长期电线积冰观测和电力部门线路覆冰调查资料,对贵州冬季电线积冰的分布、类型特征与气象条件进行了综合分析,着重探讨了贵州电线积冰的形成机理和天气成因,主要结论:(1)贵州电线积冰的类型复杂多样,除具有雨凇、雾凇、雪凇(湿雪)三种基本类型外,还有雨雾混合凇、雨雪混合凇(雪凝)两种混合冻结类型;贵州电线积冰以危售最大的雨凇冰为主,混合冻结在贵州省分布十分普遍,这也是造成2008年贵州电网严重冰害的重要原因;(2)持续低温阴雨的凝冻大气是贵州电线积冰总的天气成因,其表现形式主要是冻雨但不局限于冻雨,此外还包括冻毛雨、雨夹雪、湿雪和过冷雾等多种天气现象,持续的凝冻天气过程往往有多种天气现象同时或交替出现,从而导致严重的覆冰灾害;(3)贵州电线积冰总体上属于较高环境温度下的低风速湿物理过程积冰,当地面气温持续降到0℃以下,出现降雨或浓雾是电线积冰开始与增长的关键气象条件,而最高温度上升到0℃以上是判断积冰转入消融的临界气象指标。

关键词：电线积冰导线覆冰积冰机理天气成因

Wheat genomic study for genetic improvement of traits in China

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Science China(Life Sciences) 2022年第9期65卷 1718-1775页

作者： Jun Xiao Bao Liu Yingyin Yao Zifeng Guo Haiyan Jia Lingrang Kong Aimin Zhang Wujun Ma Zhongfu Ni Shengbao Xu Fei Lu Yuannian Jiao Wuyun Yang Xuelei Lin Silong Sun Zefu Lu Lifeng Gao Guangyao Zhao shuanghe cao Qian Chen Kunpu Zhang Mengcheng Wang Meng Wang Zhaorong Hu Weilong Guo Guoqiang Li Xin Ma Junming Li Fangpu Han Xiangdong Fu Zhengqiang Ma Daowen Wang Xueyong Zhang Hong-Qing Ling Guangmin Xia Yiping Tong Zhiyong Liu Zhonghu He Jizeng Jia Kang Chong Key Laboratory of Plant Molecular Physiology Institute of BotanyChinese Academy of SciencesBeijing 100093China Institute of Crop Sciences Chinese Academy of Agricultural SciencesBeijing 100081China The State Key Laboratory of Plant Cell and Chromosome Engineering Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China State Key Laboratory for Agrobiotechnology and Key Laboratory of Crop Heterosis and Utilization(MOE)and Beijing Key Laboratory of Crop Genetic Improvement China Agricultural UniversityBeijing 100193China The Key Laboratory of Plant Development and Environment Adaptation Biology Ministry of EducationSchool of Life ScienceShandong UniversityQingdao 266237China State Key Laboratory of Crop Biology College of AgronomyShandong Agricultural UniversityTai’an 271018China Crop Genomics and Bioinformatics Center and National Key Laboratory of Crop Genetics and Germplasm Enhancement Nanjing Agricultural UniversityNanjing210095China Key Laboratory of Molecular Epigenetics Northeast Normal UniversityChangchun 130024China College of Agronomy State Key Laboratory of Wheat and Maize Crop Scienceand Center for Crop Genome EngineeringHenan Agricultural UniversityZhengzhou 450002China State Key Laboratory of Crop Stress Biology for Arid Areas College of AgronomyNorthwest A&F UniversityYangling 712100China College of Agronomy Qingdao Agricultural UniversityQingdao 266109China Ministry of Education Key Laboratory of Molecular and Cellular Biology Hebei Collaboration Innovation Center for Cell SignalingHebei Key Laboratory of Molecular and Cellular BiologyCollege of Life SciencesHebei Normal UniversityShijiazhuang 050024China Institute of Crop Research Sichuan Academy of Agricultural SciencesChengdu 610066China State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China State Key Laboratory of Soil and Sustainable Agriculture Institute of Soil ScienceChinese Academy of Sciences

Bread wheat(Triticum aestivum L.)is a major crop that feeds 40%of the world’s *** the past several decades,advances in genomics have led to tremendous achievements in understanding the origin and domestication of wheat,and the genetic basis of agronomically important traits,which promote the breeding of elite *** this review,we focus on progress that has been made in genomic research and genetic improvement of traits such as grain yield,end-use traits,flowering regulation,nutrient use efficiency,and biotic and abiotic stress responses,and various breeding strategies that contributed mainly by Chinese *** genomic research in wheat is entering a new era with the availability of multiple reference wheat genome assemblies and the development of cutting-edge technologies such as precise genome editing tools,highthroughput phenotyping platforms,sequencing-based cloning strategies,high-efficiency genetic transformation systems,and speed-breeding *** insights will further extend our understanding of the molecular mechanisms and regulatory networks underlying agronomic traits and facilitate the breeding process,ultimately contributing to more sustainable agriculture in China and throughout the world.

关键词： wheat genomics genetic improvement China