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Maternal supply of cysteamine alleviates oxidative stress and enhances angiogenesis in porcine placenta

Journal of Animal Science and Biotechnology 2022年第1期13卷 181-196页

作者： shuangbo huang Zifang Wu Zihao huang Xiangyu Hao Longmiao Zhang Chengjun Hu Jianfu Wei Jinping Deng Chengquan Tan Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center for Breeding Swine IndustryInstitute of Subtropical Animal Nutrition and FeedCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Guangzhou DaBeiNong Agri-animal Huabandry Science and Technology Co. Ltd.Guangzhou 510642GuangdongChina Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510642GuangdongChina

Background:Oxidative stress in placenta is associated with the occurrence of adverse pregnancy outcomes in sow,but there are few satisfactory treatment strategies for these conditions.This study investigated the potential of cysteamine(CS)as an antioxidant protectant for regulating the reproductive performance,redox status,and placental angiogenesis of sows.Methods:The placental oxidative stress status and vascular density of piglets with different birth weights:<1.0 kg(low birth weight,LBW)and 1.4–1.6 kg(normal birth weight,NBW)were evaluated,followed by allotting 84 sows to four treatments(n=21)and feeding them with a basal diet supplemented with 0,100,300,or 500 mg/kg of CS from d 85 of gestation to d 21 of lactation,respectively.Placenta,serum,and colostrum samples of sows or piglets were collected,and the characteristics of sows and piglets were recorded.Furthermore,the in vivo results were validated using porcine vascular endothelial cells(PVECs).Results:Compared with the NBW placentae,the LBW placentae showed increased oxidative damage and were vulnerable to angiogenesis impairment.Particularly,H2O2-induced oxidative stress prompted intracellular reactive oxygen species generation and inhibited the tube formation and migration of PVECs as well as the expression of vascular endothelial growth factor-A(VEGF-A)in vitro.However,dietary CS supplementation can alleviate oxidative stress and improve the reproductive performance of sows.Specifically,compared with the control group,dietary 100 mg/kg CS could(1)decrease the stillbirth and invalid rates,and increase both the piglet birth weight in the low yield sows and the placental efficiency;(2)increase glutathione and reduce malondialdehyde in both the serum and the colostrum of sows;(3)increase the levels of total antioxidant capacity and glutathione in LBW placentae;(4)increase the vascular density,the mRNA level of VEGF-A,and the immune-staining intensity of platelet endothelial cell adhesion molecule-1 in the LBW placentae.Furthermore,the in vitro experiment

关键词： Angiogenesis Cysteamine Oxidative stress Placenta Sow

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Dietary fibers with low hydration properties exacerbate diarrhea and impair intestinal health and nutrient digestibility in weaned piglets

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Journal of Animal Science and Biotechnology 2023年第2期14卷 771-791页

作者： shuangbo huang Zhijuan Cui Xiangyu Hao Chuanhui Cheng Jianzhao Chen Deyuan Wu Hefeng Luo Jinping Deng Chengquan Tan Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center for Breeding Swine IndustryInstitute of Subtropical Animal Nutrition and FeedCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou 510642GuangdongChina Dekon Food and Agriculture Group Chengdu 610031China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou 510642GuangdongChina.

Background:This study aimed to investigate the hydration properties of different-source fibrous materials by com-paring their water-binding capacity(WBC),water swelling capacity(WSC),viscosity,and in vivo effects of selected samples on growth performance,nutrient digestibility,diarrhea,and intestinal health in weaned piglets.Methods:A total of 13 commercially available fibrous materials were first compared in chemical composition and in vitro hydration property.Subsequently,40 weaned piglets were randomized to five experimental dietary groups(8 piglets per group):control diet(a basal diet without dietary fiber,CON),basal diet supplemented with 5%microcrys-talline cellulose(MCC),5%wheat bran(WB),5%Moringa oleifera leaf powder(MOLP),or 5%sugar beet pulp(SBP),fol-lowed by analyzing their growth performance and diarrhea rate in a 28-d experiment.After the feeding experiment,anaesthetized piglets were killed,and their intestinal and colon content or plasma samples were analyzed in nutrient digestibility,intestinal morphology,intestinal barrier,short-chain fatty acids(SCFAs),and bacterial population.Results:In vitro studies showed low hydration properties for WB and MCC,while medium hydration properties for MOLP and SBP.In vivo studies indicated that compared with medium hydration property groups,low hydration prop-erty groups showed(1)exacerbated diarrhea,impaired intestinal health,and reduced apparent fecal digestibility of dry matter,gross energy,acid detergent fiber,and neutral detergent fiber;(2)decreased SCFAs concentration and rela-tive levels of Lactobacillus and Bifidobacterium,but increased levels of Escherichia coli and Brachyspira hyodysenteriae in colon contents.Additionally,SBP showed optimal performance in reducing diarrhea and increasing SCFAs produc-tion.Correlation analysis revealed a positive correlation of fiber hydration properties with in vitro SCFAs production,and diarrhea index and nutrient digestibility were negatively and positively correlated with SCFAs levels in the colon contents of weaned

关键词： Diarrhea Dietary fiber Hydration property Intestinal health Nutrient digestibility Weaned piglet

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Dietary adenosine supplementation improves placental angiogenesis in IUGR piglets by up-regulating adenosine A2a receptor

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Animal Nutrition 2023年第2期13卷 282-288页

作者： Zifang Wu Jiawei Nie Deyuan Wu shuangbo huang Jianzhao Chen Huajin Liang Xiangyu Hao Li Feng Hefeng Luo Chengquan Tan Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center for Breeding Swine IndustryInstitute of Subtropical Animal Nutrition and FeedCollege of Animal ScienceSouth China Agricultural UniversityGuangzhouGuangdong 510642China Dekon Food and Agriculture Group ChengduChina

Abnormal placental angiogenesis is associated with the occurrence of intrauterine growth restriction(IUGR)in piglets,and effective treatment strategies against this occurrence remain to be explored.Adenosine has been reported to play an important role in angiogenesis,but its role in placental angio-genesis is still unknown.Here,we investigated the effect of dietary adenosine supplementation on IUGR occurrence in piglets by analyzing the role of adenosine in placental angiogenesis for Normal and IUGR piglets.Specifically,88 sows were allotted to 2 treatments(n=44)and fed a basal diet supplemented with 0%or 0.1%of adenosine from day 65 of gestation until farrowing,followed by collecting the placental samples of Normal and IUGR piglets,and recording their characteristics.The results showed that adenosine supplementation increased the mean birth weight of piglets(P<0.05)and placental efficiency(P<0.05),while decreasing the IUGR piglet rate(P<0.05).Expectedly,the placenta for IUGR neonates showed a down-regulated vascular density(P<0.05)and angiogenesis as evidenced by the expression level of vascular cell adhesion molecule-1(VCAM1)(P<0.05).Notably,dietary adenosine supplementation promoted angiogenesis(P<0.05)both in the Normal and IUGR placenta.More importantly,the expression level of adenosine A2a receptor(ADORA2A)was lower(P<0.05)in the IUGR placenta than in Normal placenta,whereas adenosine treatment could significantly increase ADORA2A expression,and also had an interaction effect between factors IUGR and Ado.Collectively,placentae for IUGR piglets showed impaired angiogenesis and down-regulated expression level of ADORA2A,while dietary adenosine supplementation could activate ADORA2A expression,improve the placental angio-genesis,and ultimately decrease the occurrence of IUGR in piglets.

关键词： Angiogenesis IUGR Placenta Adenosine ADORA2A

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Inclusion of wheat aleurone in gestation diets improves postprandial satiety,stress status and stillbirth rate of sows

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Animal Nutrition 2021年第2期7卷 412-420页

作者： Jinping Deng Chuanhui Cheng Haoyuan Yu shuangbo huang Xiangyu Hao Jianzhao Chen Jiansen Yao Jianjun zuo Chengquan Tan Guangdong Provincial Key Laboratory of Animal Nutrition Control National Engineering Research Center for Breeding Swine IndustryInstitute of Subtropical Animal Nutrition and FeedCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou510642China Guangdong Laboratory for Lingnan Modern Agriculture South China Agricultural UniversityGuangzhou510642China Jiaxing Zhishifang Food Science and Technology Co. Ltd.JiaxingChina

This study investigated the effects of different amounts of wheat aleurone(WA)(0,15%,30%)inclusion in gestation diets on the reproductive performance,postprandial satiety,stress status and stereotypic be-haviors of sows.A total of 84 Landrace×Yorkshire sows(parity 4.87±1.32)at breeding were randomly allotted to one of the three isoenergetic and isonitrogenous dietary treatments based on parity and body weight.The results showed that,compared with the control(0),sows fed the WA diet had a higher serum concentration of peptide YY(PYY)(P<0.05)and glucagon like peptide-1(GLP-1)(P<0.05)and a lower concentration of saliva cortisol(P<0.01).Importantly,compared with the control group,only the 15%WA group had a higher concentration of the total antioxidant capacity(T-AOC)(P<0.05),lower pro-portions of sitting(P=0.05)and stillbirth rates(P<0.01).Accordingly,the production cost per piglet born alive($6.9 vs.$7.6)or per piglet born healthy($7.4 vs.$7.9)declined in the 15%WA group versus the control group.Overall,15%WA inclusion in gestation diets contributed to enhancing postprandial satiety,alleviating stress status and decreasing stillbirth rate of sows.This study provides a reference for the application of WA as a partial substitute for conventional feed ingredients to improve sows'repro-ductive performance.

关键词： Wheat aleurone Stillbirth rate Stress Postprandial satiety Sow

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Effects of dietary supplementation of nucleotides from late gestation to lactation on the performance and oxidative stress status of sows and their offspring

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Animal Nutrition 2021年第1期7卷 111-118页

作者： Chengquan Tan Yongcheng Ji Xichen Zhao Zhongquan Xin Jiaying Li shuangbo huang Zhiying Cui Lijun Wen Caihua Liu Sung Woo Kim Jinping Deng Yulong Yin Guangdong Laboratory of Lingnan Modern Agriculture Guangdong Provincial Key Laboratory of Animal Nutrition ControlNational Engineering Research Center for Breeding Swine IndustryCollege of Animal ScienceSouth China Agricultural UniversityGuangzhou510642China National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production Institute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China Department of Animal Science North Carolina State University RaleighNC27695USA Guangdong Hinabiotech Co. Ltd.Guangzhou510642China Hubei Hiyee Biological Technology Co. Ltd.Xiangyang510642China

Increased metabolic burdens in breeding sows,which are induced by elevated systemic oxidative stress,could increase the need for nucleotides to repair lymphocyte DNA damage;however,de novo synthesis of nucleotides may be insufficient to cover this increased need.This study investigated the effects of dietary nucleotides on milk composition,oxidative stress status,and the reproductive and lactational performance of sows.Forty multiparous sows were assigned to 2 dietary treatments(Control group,and 1 g/kg Nucleotides group)based on a randomized complete block design using their BW at 85 d of gestation as a block.Sows from 2 groups were fed a restricted diet during gestation and ad libitum during lactation.The experiment lasted from 85 d of gestation to 21 d of lactation.The reproductive perfor-mance of sows and the growth performance of suckling piglets were measured.Oxidative stress pa-rameters and milk components were also analysed.Data were analyzed using contrasts in the MIXED procedure of SAS.Sows in the Nucleotides group consumed more feed during the first week(P<0.01)and from 1 to 21 d(P<0.05)of lactation than those in Control group.Correspondingly,the litter weight gain of piglets showed a tendency to increase from cross-fostering to 9 d(P=0.09)and from cross-fostering to 20 d(P=0.10)in the Nucleotides group relative to the Control group.Additionally,the Nucleotides group was higher(P<0.01)than the Control group in the concentrations of uridine 5'monophosphate,guanosine 5'monophosphate,inosine 5'monophosphate,adenosine 5'mono-phosphate and total nucleotides in milk.Furthermore,the Nucleotides group was higher(P<0.01)than the Control group in the serum levels of total antioxidant capacity(P<0.01)for sows at 109 d of gestation and glutathione peroxidase for weaning piglets,but lower at the levels of thiobarbituric acid-reactive substances(P<0.05)in serum of weaning piglets.This study indicated that maternal dietary nucleotides could promote piglet growth,probably due to the higher lactational feed intake and

关键词： Feed intake Nucleotide Oxidative stress Piglet Sow

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克唑替尼相关性肾囊肿1例

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中华结核和呼吸杂志 2024年第4期47卷 356-359页

作者：全玲丽黄彭梁彦超陈芳玮刘双柏辛雯雯株洲市中心医院/中南大学湘雅医学院附属株洲医院呼吸与危重症医学科株洲412007 株洲市中心医院/中南大学湘雅医学院附属株洲医院病理科株洲412007

克唑替尼相关性肾囊肿(CARC)是指在使用克唑替尼治疗后新发肾囊肿或先前存在的肾囊肿发生变化,停用克唑替尼后,大多数CARC会自行消退。少数CARC表现出侵袭性行为,可超过肾皮质侵入附近结构,包括肾周间隙、腰大肌、肠道和腹壁等。本文报... 详细信息

克唑替尼相关性肾囊肿(CARC)是指在使用克唑替尼治疗后新发肾囊肿或先前存在的肾囊肿发生变化,停用克唑替尼后,大多数CARC会自行消退。少数CARC表现出侵袭性行为,可超过肾皮质侵入附近结构,包括肾周间隙、腰大肌、肠道和腹壁等。本文报道了1例浸润性肺腺癌EML4-ALK融合突变,给予克唑替尼治疗后反复出现双肾、右侧竖直肌、腰大肌多发囊性改变,停用药物后自行吸收消退过程。

关键词：克唑替尼肾囊肿肾周间隙腰大肌肾皮质 CAR 囊性改变相关性

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Error Analysis of Approximate Calculation of Voltage Divider Biased Common-Emitter Amplifier

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Circuits and Systems 2017年第10期8卷 247-252页

作者： Xinwu Chen Jingjing Xue shuangbo Xie Wenxia huang Peng Wang Ke Gong Lijuan Zhong School of Physics and Electronic Engineering Xinyang Normal University Xinyang China

Voltage divider biasing common emitter amplifier is one of the core contents in analog circuit curriculum, and almost all of traditional textbooks apply approximate calculation method to estimate all characteristic parameters. In calculating quiescent point, transistor base current is generally ignored to get the approximate base potential and emitter current, then other operating parameters, and AC small signal parameters can be acquired. The main purpose of this paper is to compare traditional and Thevenin equivalent methods and to get the difference of the two methods. A Formula is given to calculate the error of the traditional method. Example calculating reveals that the traditional method can generate an error about 10%, and even severe for small signal amplifier with higher quiescent point.

关键词： Common-Emitter Amplifier Quiescent Point Thevenin Equivalent Circuit Small Signal Amplifier

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侵袭性肺炎克雷伯菌肝脓肿综合征12例临床特征分析

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中华全科医师杂志 2023年第9期22卷 954-959页

作者：刘斌梁彦超刘双柏陈芳玮谢波黄彭株洲市中心医院中南大学湘雅医学院附属株洲医院呼吸与危重症医学科株洲412007 株洲市中心医院中南大学湘雅医学院附属株洲医院医务部质控办株洲412007

目的总结分析侵袭性肺炎克雷伯菌肝脓肿综合征(IKLAS)患者的临床特征,以提高对该病的认识。方法回顾性分析株洲市中心医院2020年1月至2023年1月确诊为IKLAS的12例患者的临床资料。结果12例患者中男性6例,女性6例,年龄49~90(65.3±12.2)... 详细信息

目的总结分析侵袭性肺炎克雷伯菌肝脓肿综合征(IKLAS)患者的临床特征,以提高对该病的认识。方法回顾性分析株洲市中心医院2020年1月至2023年1月确诊为IKLAS的12例患者的临床资料。结果12例患者中男性6例,女性6例,年龄49~90(65.3±12.2)岁,9例合并糖尿病。主要临床表现为发热(9例),畏寒(6例),寒战(4例),恶心、呕吐(2例),上腹痛(2例),乏力、纳差(2例),咳嗽、咳痰(1例),意识障碍(1例),咯血(1例)。8例患者白细胞计数水平升高,10例淋巴细胞计数减少,3例血小板计数减少。所有患者C反应蛋白、降钙素原水平均升高。所有患者血清白蛋白水平均降低。7例患者丙氨酸氨基转移酶水平升高,7例门冬氨酸氨基转移酶水平升高。8例患者肝脓肿位于肝右叶,1例位于肝左叶,3例发生于双侧;7例为单发脓肿,5例为多发脓肿。病原学标本主要来源于肝脓液和血液培养,分别有10例和5例阳性。侵袭部位或系统主要为肺和血液系统,分别为10例和5例。12例患者培养鉴定的肺炎克雷伯菌多为敏感菌株,总体耐药率低。所有患者经抗感染治疗,其中10例患者行肝脓肿穿刺引流。11例患者治疗后好转出院,1例因脓毒症休克死亡。结论IKLAS的临床症状多样,多合并糖尿病,主要侵袭肺和血液系统,及时诊断、积极筛查肝外其他转移性感染部位、及时脓肿引流和合适的抗菌药物治疗可提高患者生存率。

关键词：克雷伯菌,肺炎肝脓肿临床特征

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二代测序协助诊断多杀巴斯德菌脓胸1例

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中南大学学报（医学版） 2021年第8期46卷 920-924页

作者：刘斌黄彭刘双柏梁彦超陈芳玮全玲丽罗细萍周娟株洲市中心医院/中南大学湘雅医学院附属株洲医院呼吸与危重症医学科一区湖南株洲412007

多杀巴斯德菌脓胸少见,容易被漏诊。1例81岁患者因"咳嗽、咳痰、发热3 d"入院,诊断为"社区获得性肺炎",经抗感染治疗后仍发热,胸X射线摄影检查提示胸腔积液,行胸腔闭式引流,引流出红褐色液体,将胸腔积液行二代测序,检测出多杀巴斯德菌... 详细信息

多杀巴斯德菌脓胸少见,容易被漏诊。1例81岁患者因"咳嗽、咳痰、发热3 d"入院,诊断为"社区获得性肺炎",经抗感染治疗后仍发热,胸X射线摄影检查提示胸腔积液,行胸腔闭式引流,引流出红褐色液体,将胸腔积液行二代测序,检测出多杀巴斯德菌。多杀巴斯德菌对生长条件要求比较严格,是一种难以培养的细菌,而二代测序的应用有助于快速诊断病原体。

关键词：二代测序多杀巴斯德菌脓胸

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肥胖和支气管哮喘之间的因果关联:一项双向孟德尔随机化研究

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国际呼吸杂志 2023年第12期43卷 1421-1427页

作者：刘斌梁彦超刘双柏陈芳玮罗细萍周娟徐藤娟谢波黄彭株洲市中心医院、中南大学湘雅医学院附属株洲医院呼吸与危重症医学科一区株洲412007 株洲市中心医院、中南大学湘雅医学院附属株洲医院医务部质控办株洲412007

目的探讨肥胖与支气管哮喘(哮喘)的因果关联,为哮喘的防治提供参考。方法本研究为双向孟德尔随机化研究。通过与体质量指数(BMI)、哮喘相关的2项全基因组关联研究,收集单核苷酸多态性(SNP)资料。采用逆方差加权法随机效应模型(IVW-RE)... 详细信息

目的探讨肥胖与支气管哮喘(哮喘)的因果关联,为哮喘的防治提供参考。方法本研究为双向孟德尔随机化研究。通过与体质量指数(BMI)、哮喘相关的2项全基因组关联研究,收集单核苷酸多态性(SNP)资料。采用逆方差加权法随机效应模型(IVW-RE)、孟德尔随机化Egger回归法(MR-Egger)、加权中位数法和加权众数法。以BMI相关的370个SNP作为工具变量,以哮喘作为研究结局进行正向孟德尔随机化(MR)分析。以哮喘相关的56个SNP作为工具变量,以BMI作为研究结局进行反向MR分析。结果IVW-RE结果显示,BMI与哮喘具有显著的因果关联,BMI每增加1个标准差,哮喘发病风险约增加1.26倍(OR=1.26,95%CI:1.19~1.33,P<0.001),在MR-Egger(OR=1.17,95%CI:1.01~1.36,P=0.035)、加权中位数法(OR=1.20,95%CI:1.11~1.29,P<0.001)和加权众数法(OR=1.15,95%CI:1.03~1.29,P=0.015)分析中同样发现BMI与哮喘具有正向因果关联。此外,使用IVW-RE进行反向MR分析,没有发现哮喘对BMI有影响的证据(OR=0.99,95%CI:0.98~1.00,P=0.193)。结论MR分析表明,成人BMI与哮喘的患病率存在因果关系,而哮喘对成人BMI的影响可能性很小。

关键词：哮喘体质量指数孟德尔随机化全基因组关联研究

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