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PM_(2.5) obtained from urban areas in Beijing induces apoptosis by activating nuclear factor-kappa B

Military Medical Research 2017年第4期4卷 200-209页

作者： Hui peng Xiao-Hong Zhao Ting-Ting Bi Xiao-Yan Yuan Jia-Bin Guo shuang-qing peng Evaluation and Research Center for Toxicology Institute of Disease Control and Prevention Academy of Military Medical Sciences Beijing Key Laboratory of Bioactive Substances and Functional Foods Beijing Union University

Background: Particulate matter(PM), which has adverse effects on citizen health, is a major air pollutant in Beijing city. PM_(2.5) is an indicator of PM in urban areas and can cause serious damage to human health. Many epidemiological studies have shown that nuclear factor-kappa B(NF-κB) is involved in PM_(2.5)-induced cell injury, but the exact mechanisms are not well ***: The cytotoxic effects of PM_(2.5) at 25–1600μg/ml for 24 h were determined by MTT assay in Chinese hamster ovary cells(CHO) cells. Flow cytometry was used to determine the apoptosis rate induced by PM_(2.5). The destabilized enhanced green fluorescent protein(d2 EGFP) green fluorescent protein reporter system was used to determine the NF-κB activity induced by PM_(2.5). The expression of pro-apoptotic Bcl-2-associated death promoter(BAD) proteins induced by PM_(2.5) was determined by Western blotting to explore the relationship between PM_(2.5) and the NF-κB signaling pathway and to determine the toxicological mechanisms of PM_(2.5).Results: PM_(2.5) collected in Beijing urban districts induces cytotoxic effects in CHO cells according to MTT assay with 72.28% cell viability rates even at 200μg/ml PM_(2.5) and flow cytometry assays with 26.97% apoptosis rates at 200μg/ml PM_(2.5). PM_(2.5) increases the activation levels of NF-κB, which have maintained for 24 h. 200μg/ml PM_(2.5) cause activation of NF-κB after exposure for 4 h, the activation peak appears after 13.5 h with a peak value of 25.41%. The average percentage of NF-κB activation in whole 24 h is up to 12.90% by 200μg/ml PM_(2.5). In addition, PM_(2.5) decreases the expression level of the pro-apoptotic protein BAD in a concentration-dependent ***: PM_(2.5) induces NF-κB activation, which persists for 24 h. The expression of pro-apoptotic protein BAD decreased with increased concentrations of PM_(2.5). These findings suggest that PM_(2.5) plays a major role in apoptosis by activating the NF-κB signaling path

关键词： Particulate matter NF-κB pathway Apoptosis BAD protein

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广防己的肾毒性及代谢组学研究

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中西医结合学报 2009年第8期7卷 746-752页

作者：梁琦倪诚谢鸣张琪张艳霞颜贤忠杨美娟彭双清张宇忠北京中医药大学方剂学学科北京100029 国家生物医学分析中心北京100850

目的:采用代谢组学方法研究大鼠予广防己灌胃后尿液代谢产物的改变情况。方法:雄性SD大鼠64只随机分为广防己组和正常对照组,每组32只。广防己组大鼠予广防己8.1g/(kg.d)灌胃,正常对照组灌服等体积蒸馏水,连续灌胃4周。在灌胃前,灌胃2周... 详细信息

目的:采用代谢组学方法研究大鼠予广防己灌胃后尿液代谢产物的改变情况。方法:雄性SD大鼠64只随机分为广防己组和正常对照组,每组32只。广防己组大鼠予广防己8.1g/(kg.d)灌胃,正常对照组灌服等体积蒸馏水,连续灌胃4周。在灌胃前,灌胃2周、4周及停药2周后收集大鼠24h尿液,利用核磁共振(nuclear magnetic resonance,NMR)技术测定大鼠尿液代谢产物1HNMR谱,进行模式识别分析;取血检测血尿素氮(blood urea nitrogen,BUN)和血清肌酐(serum creatinine,SCr)含量;取肾脏组织行病理学检查。结果:与正常对照组比较,给药2周时广防己组大鼠血BUN含量明显升高(P<0.05);肾组织出现肾小管细胞肿胀,肾小球球囊结构破坏,肾间质炎性细胞浸润。给药4周时,BUN和SCr含量显著升高(P<0.05),肾组织病理改变加重。停药恢复2周后,广防己组BUN含量仍较正常对照组明显升高(P<0.05),且病理改变与给药4周时无明显差异。大鼠尿样代谢谱经主成分分析提示,广防己组与正常对照组在各时间点代谢物图谱均有差异,整个给药期间,广防己组柠檬酸含量持续下降,牛磺酸含量持续上升。在给药2周时,伴马尿酸盐含量上升;给药4周时伴马尿酸盐、2-酮戊二酸含量下降,氧化三甲胺和肌酐/肌酸含量上升;停药恢复2周后肌酐/肌酸和2-酮戊二酸含量下降,马尿酸盐和氧化三甲胺含量上升。结论:10倍药典剂量广防己给药2周可造成肾功能损害,随给药时间延长,损伤加重,停药后有一定恢复。广防己可能还具有一定的肝脏毒性作用。

关键词：广防己肾毒性代谢组学核磁共振模式识别大鼠

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A cellulose-based lithium-ion battery separator with regulated ionic transport and high thermal stability for extreme environments

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Nano Research 2025年第1期18卷 315-322页

作者： Kun-peng Yang Song Xie Zi-Meng Han Hao-Cheng Liu Chong-Han Yin Wen-Bin Sun Zhang-Chi Ling Huai-Bin Yang De-Han Li qing-Fang Guan Shu-Hong Yu Department of Chemistry New Cornerstone Science LaboratoryInstitute of Biomimetic Materials&ChemistryAnhui Engineering Laboratory of Biomimetic MaterialsDivision of Nanomaterials&ChemistryHefei National Research Center for Physical Sciences at the MicroscaleUniversity of Science and Technology of ChinaHefei 230026China Institute of Innovative Materials Department of Materials Science and EngineeringDepartment of ChemistrySouthern University of Science and TechnologyShenzhen 518055China

Separators play a critical role in lithium-ion ***,the restrictions of thermal stability and inferior electrical performance in commercial polyolefin separators significantly limit their applications under harsh ***,we report a cellulose-assisted self-assembly strategy to construct a cellulose-based separator massively and *** an ultrahigh ionic conductivity in electrolytes of 3.7 mS·cm^(-1) and the ability to regulate ion transport,the obtained separator is a promising alternative for high-performance lithium-ion *** addition,integrated with high thermal stability,the cellulose-based separator endows batteries with high safety at high temperatures,greatly expanding the application scenarios of energy storage devices in extreme environments.

关键词： lithium-ion batteries bio-inspired materials battery separators cellulose nanofiber ultrahigh ionic conductivity high safety

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Structure-based Screening for the Non-zinc-chelating Selective MMP-13 Inhibitors of Natural Products

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Chinese Journal of Structural Chemistry 2020年第11期39卷 1990-2000页

作者： ZOU qing GAO Qiu-shuang YAO peng YAO Qi-Zheng ZHANG Ji Department of Physical Chemistry China Pharmaceutical UniversityNanjing 210009China State Key Laboratory of Natural Medicines China Pharmaceutical UniversityNanjing 210009China School of Pharmacy China Pharmaceutical UniversityNanjing 210009China

Matrix metalloproteinase-13(MMP-13) has been considered as a promising therapeutic target for osteoarthritis. In this work, the experimental crystal structures of five MMP-13-ligand complexes are used to build the multiple structure-based pharmacophore model of MMP-13 inhibitors. The reliability of pharmacophore model is validated using a decoy set. The pharmacophore model contains four chemical features: two hydrogen bond acceptor(HBA), one hydrophobic(HY) feature, and one ring aromatic(RA) feature. Particularly, the HY feature is found to orient the MMP-13 inhibitors deep into the S1’ pocket of MMP-13 to produce selective inhibition. By carrying out the screening of pharmacophore model and subsequent molecular docking, the four non-zinc-chelating selective MMP-13 inhibitors of natural products(NP-015973, NP-000814, STOCK1 N-24933, and STOCK1 N-69443) are identified. It is found that the binding modes of MMP-13 with our screened four natural products are very similar to the reported experimental binding mode of MMP-13 with the most active inhibitor(GG12003, IC50: 0.67 n M), and each of them involves the interactions of a ligand with the three amino acid residues Thr226, Lys119, and His201 of MMP-13 receptor. This shows that our modeling results are in good agreement with the relevant experimental results, which strongly supports our screened MMP-13 inhibitors of natural products. These screened natural products may be used as the lead compounds of MMP-13 inhibitors in the future studies of structural modifications.

关键词： matrix metalloproteinase-13 non-zinc-chelating inhibitor of MMP-13 pharmacophore model molecular docking screening

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Tailoring thermal conductivity of bulk graphene oxide by tuning the oxidation degree

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Chinese Chemical Letters 2018年第5期29卷 711-715页

作者： qing-Long Meng Hengchang Liu Zhiwei Huang shuang Kong peng Jiang Xinhe Bao State Key Laboratory of Catalysis CAS Center for Excellence in Nanoscience Dalian Institute of Chemical Physics Chinese Academy of Sciences Dalian 116023 China School of Physical Science and Technology Shanghai Tech University Shanghai 200031 China University of Chinese Academy of Sciences Beijing 100039 China

Bulk graphene oxide （GO） shows great potential in a variety of applications, such as sensors,photodetectors, supercapacitors, lithium ion batteries and catalysts. However, its thermal conductivity,one of the most important and fundamental physical properties, is still less known. Herein, we havesystematically investigated the thermal conductivity of bulk GOs and find that it can be tailored by tuningtheir oxidation degree during preparation process. Notably, the cross-plane thermal conductivity of bulkGO, in comparison with its precursor graphite, exhibits more than 100 times decrease at roomtemperature. The dependence of thermal conductivity of GO on oxidation degree is attributed to thechemical and structural changes by introducing oxygen atoms and oxygen-containing functional groups,which can lead to a significant enhancement in atomic- and nano-scale phonon scattering. Furthermore,we reveal that the thermal conductivity of bulk GOs exhibits evident anisotropic behavior. These resultsprovide fundamental understanding and valuable information on thermal transport properties of bulkGOs for various practical applications.

关键词： Graphene oxide Thermal conductivity Scattering center Anisotropy Oxidation degree

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18CrNiMo7-6钢的热处理工艺研究

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热处理技术与装备 2017年第4期38卷 29-33页

作者：王全振张忠和刘爽庆邹鹏王飞宇程亮沈阳鼓风机集团股份有限公司辽宁沈阳110869

本文对18CrNiMo7-6渗碳钢进行了不同热处理工艺试验,预备热处理采用调质、正回火工艺,渗碳后采用一次淬火、二次淬火工艺进行对比。采用金相法检验预备热处理后的金相组织、渗碳后的心部组织,和碳化物级别、马氏体、残余奥氏体、内氧化... 详细信息

本文对18CrNiMo7-6渗碳钢进行了不同热处理工艺试验,预备热处理采用调质、正回火工艺,渗碳后采用一次淬火、二次淬火工艺进行对比。采用金相法检验预备热处理后的金相组织、渗碳后的心部组织,和碳化物级别、马氏体、残余奥氏体、内氧化的评级等;同时对预备热处理后材料的力学性能进行了检验。结果表明:采用调质预备热处理、渗碳后二次淬火工艺的18CrNiMo7-6渗碳钢的渗碳层性能指标最好,适用于高参数齿轮渗碳淬火。

关键词：高速齿轮 18CrNiMo7-6钢渗碳淬火工艺

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Predicting lymph node metastasis in colorectal cancer:An analysis of influencing factors to develop a risk model

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World Journal of Gastrointestinal Surgery 2023年第10期15卷 2234-2246页

作者： Yun-peng Lei qing-Zhi Song shuang Liu Ji-Yan Xie Guo-qing Lv Department of Gastrointestinal Surgery Peking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina Department of Gastrointestinal Surgery Shenzhen Peking University-The Hong Kong University of Science and Technology Medical CenterPeking University Shenzhen HospitalShenzhen 518036Guangdong ProvinceChina

BACKGROUND Colorectal cancer(CRC)is a significant global health issue,and lymph node metastasis(LNM)is a crucial prognostic *** prediction of LNM is essential for developing individualized treatment strategies for patients with ***,the prediction of LNM is challenging and depends on various factors such as tumor histology,clinicopathological features,and molecular *** most reliable method to detect LNM is the histopathological examination of surgically resected specimens;however,this method is invasive,time-consuming,and subject to sampling errors and interobserver *** To analyze influencing factors and develop and validate a risk prediction model for LNM in CRC based on a large patient *** This study retrospectively analyzed 300 patients who underwent CRC surgery at two Peking University Shenzhen hospitals between January and December 2021.A deep learning approach was used to extract features potentially associated with LNM from primary tumor histological images while a logistic regression model was employed to predict LNM in CRC using machine-learning-derived features and clinicopathological variables as *** The prediction model constructed for LNM in CRC was based on a logistic regression framework that incorporated machine learning-extracted features and clinicopathological *** model achieved high accuracy(0.86),sensitivity(0.81),specificity(0.87),positive predictive value(0.66),negative predictive value(0.94),area under the curve for the receiver operating characteristic(0.91),and a low Brier score(0.10).The model showed good agreement between the observed and predicted probabilities of LNM across a range of risk thresholds,indicating good calibration and clinical *** The present study successfully developed and validated a potent and effective risk-prediction model for LNM in patients with *** model utilizes machine-learning-derived features extracted from pr

关键词： Colorectal cancer Lymph node metastasis Machine learning Risk prediction model Clinicopathological factors Individualized treatment strategies

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Phenotypic analysis of a dwarf and deformed flower3 (ddf3) mutant in rice (Oryza sativa L.) and characterization of candidate genes

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Journal of Integrative Agriculture 2018年第5期17卷 1057-1065页

作者： WANG Yu-peng TANG shuang-qin WU Zhi-feng SHI qing-hua WU Zi-ming Key Laboratory of Crop Physiology Ecology and Genetic BreedingMinistry of Education/Collaborative Innovation Center for the Modernization Production of Double Cropping Rice/College of AgronomyJiangxi Agricultural University

Dwarf mutants are the crucial resources for molecular biology research and rice breeding. Here, a rice mutant, dwarf and deformed flower3（ddf3）, was identified in tissue culture of Oryza sativa cv. Dongjin. Compared with wild type, the ddf3 mutant exhibited severe dwarfism, a greater number of tillers and significantly decreased fertility. In addition, leaf length, panicle length, and grain length, were significantly shorter. All internodes of ddf3 were shorter than those of wild type, and histological analysis revealed that internode cell elongation was significantly inhibited in ddf3. In the ddf3 mutant, pollen activity was significantly decreased, and the development of most stigmas was abnormal. Genetic analysis indicated that the ddf3 mutant phenotypes are controlled by a single or tightly linked nuclear genes. Using an F2 mapping population generated from a cross between ddf3 and Yangdao 6（9311）, the DDF3 gene was mapped to a 45.21-kb region between insertion-deletion（In Del） markers M15 and M16 on the long arm of chromosome 7. Sequencing revealed a 13.98-kbdeletion in this region in the ddf3 mutant genome that resulted in the complete or partial deletion of ZF（DHHC type zinc finger protein）, EP（expressed protein）, and FH2（actin-binding FH2 domain-containing protein） genes. Quantitative RT-PCR analyses revealed that in wild type, the transcript levels of FH2 were almost the same in all organs, while ZF was mainly expressed in the panicle, and no expression of EP was detected in any organ. Based on these results, ZF and FH2 could be potential DDF3 candidate genes involved in the regulation of rice morphology and flower organ development. Our work has laid the foundation for future functional analysis of these candidate genes and has provided a profitable gene resource for rice breeding for increased fertility in the future.

关键词： rice dwarf and deformed flower 3 mapping DHHC-type actin-binding FH2 domain

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Fuzzy Control of Spacecraft Attitude Maneuver with Actuator Saturation 36

Fuzzy Control of Spacecraft Attitude Maneuver with Actuator ...

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第36届中国控制会议

作者： qing Geng peng Li Ting Zhang shuang Ju Hongjiu Yang Institute of Electrical Engineering Yanshan University College of Science Yanshan University

ISBN: (纸本)9781538629185

Spacecraft attitude control is investigated via fuzzy control systems subject to actuator saturation and delta operator *** spacecraft attitude control model is transformed into a fuzzy delta operator system with appropriate fuzzy *** stability of fuzzy delta operator systems with actuator saturation is studied via Lyapunov *** a sufficient condition on stability for fuzzy delta operator systems is given and a fuzzy controller is ***,an example of spacecraft attitude control is considered to show the effectiveness of the proposed results.

关键词： Stability analysis spacecraft attitude control actuator saturation delta operator T-S fuzzy system

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Electrically tunable planar liquid-crystal singlets for simultaneous spectrometry and imaging

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Light(Science & Applications) 2024年第11期13卷 2589-2598页

作者： Zhou Zhou Yiheng Zhang Yingxin Xie Tian Huang Zile Li peng Chen Yan-qing Lu Shaohua Yu shuang Zhang Guoxing Zheng Electronic Information School and School of MicroelectronicsWuhan UniversityWuhan 430072China NUS Graduate School National University of SingaporeSingapore 119077Singapore Department of Electrical and Computer Engineering National University of SingaporeSingapore 117583Singapore National Laboratory of Solid State Microstructures Key Laboratory of Intelligent Optical Sensing and Manipulationand College of Engineering and Applied SciencesNanjing UniversityNanjing 210093China peng Cheng Laboratory Shenzhen 518055China New Cornerstone Science Laboratory Department of PhysicsUniversity of Hong KongHong KongChina Department of Electrical and Electronic Engineering University of Hong KongHong KongChina Wuhan Institute of Quantum Technology Wuhan 430206China

Conventional hyperspectral cameras cascade lenses and spectrometers to acquire the spectral datacube,which forms the fundamental framework for hyperspectral ***,this cascading framework involves tradeoffs among spectral and imaging performances when the system is driven toward ***,we propose a spectral singlet lens that unifies optical imaging and computational spectrometry functions,enabling the creation of minimalist,miniaturized and high-performance hyperspectral *** a paradigm,we capitalize on planar liquid crystal optics to implement the proposed framework,with each liquid-crystal unit cell acting as both phase modulator and electrically tunable spectral *** with various targets show that the resulting millimeter-scale hyperspectral camera exhibits both high spectral fidelity(>95%)and high spatial resolutions(~1.7 times the diffraction limit).The proposed“two-in-one”framework can resolve the conflicts between spectral and imaging resolutions,which paves a practical pathway for advancing hyperspectral imaging systems toward miniaturization and portable applications.

关键词： crystal planar singlet

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