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IgE Reactivity of Potential Allergens from the Swimming Crab Portunus trituberculatus and the Research of ELISA Reagent for Detecting Crab Food Anaphylaxis

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Journal of Ocean University of China 2022年第2期21卷 421-429页

作者： QIU Xueni ZHOU Qiongyan ZHU Xiaoxia LIN Wei MU Changkao LI Ronghua YE yangfang SONG Weiwei SHI Ce LIU Lei WANG huan WANG Chunlin XU Suling Key Laboratory of Aquatic Biotechnology Ministry of EducationNingbo UniversityNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo UniversityNingbo 315211China The Affiliated Hospital of Medical School of Ningbo University Ningbo 315020China

Crustacean is one of the major allergic *** is of great significance to identify more crab allergens and research the detection methods for crab food *** this study,IgE reactivity to three recombinant proteins from Portunus trituberculatus,including tropomyosin(rPtTM),myosin light chain(rPtMLC),and pancreatic lipase(rPtPL),were detected by enzyme-linked im-munosorbent assay(ELISA).The expressions of tropomyosin(TM)in various tissues of *** were detected by Western blot(WB).Furthermore,microplates were coated with rPtTM and the ELISA conditions were *** cut-off value was deter-mined by the receiver operating characteristic(ROC)*** 51 crab-allergic sera,21(41.2%)showed positive IgE to *** 70 crab-allergic sera more frequently recognized rPtPL(9/70;12.9%),followed by rPtMLC(1/70;1.4%).WB results showed that TM was mainly expressed in the muscle,followed by the heart and a small amount in gills and the *** optimal results showed that the coating condition of rPtTM was 50 ng per well with coating for 3 h at 37℃.The optimal blocking condition was 1.2%BSA with blocking for 3 h at 37℃,and the optimal dilution of the second antibody was 1:*** ROC curve showed that the ELISA reagent had high sensitivity(83.52%)and specificity(98.00%)when the cut-off value was *** results indicated that tropomyosin is the major allergen of ***,and myosin light chain and pancreatic lipase are the potential ***-tionally,the ELISA reagent developed with the rPtTM was feasible for laboratory detection of crab anaphylaxis.

关键词： Portunus trituberculatus potential allergens food anaphylaxis ELISA reagent

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Growth of Gallium Nitride Films on Multilayer Graphene Template Using Plasma-Enhanced Atomic Layer Deposition

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Acta Metallurgica Sinica(English Letters) 2019年第12期32卷 1530-1536页

作者： Ying-Feng He Mei-Ling Li San-Jie Liu Hui-Yun Wei huan-Yu Ye Yi-Meng Song Peng Qiu Yun-Lai An Ming-Zeng Peng Xin-He Zheng School of Mathematics and Physics Beijing Key Laboratory for Magneto-Photoelectrical Composite and Interface ScienceUniversity of Science and Technology Beijing

In this work,the GaN thin films were directly deposited on multilayer graphene(MLG)by plasma-enhanced atomic layer *** deposition was carried out at a low temperature using triethylgallium(TEGa)precursor and Ar/N2/H2 *** properties of the bulk GaN and GaN-graphene interface were analyzed using X-ray photoelectron *** sharp interface between GaN and graphene was verified via X-ray reflectivity and transmission electron *** microstructures and the nucleation behaviors of the GaN grown on graphene have been also *** results of grazing incidence X-ray diffraction and Raman spectrum indicate that the as-deposited sample is polycrystalline with wurtzite structure and has a weakly tensile *** properties of the sample were investigated by photoluminescence(PL)at room *** successful growth of GaN on MLG at a low temperature opens up the possibility of ameliorating the performance of electronic and optical devices based on GaN/graphene heterojunction.

关键词： Plasma-enhanced atomic layer deposition Gallium nitride Graphene Interface microstructure

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大型碟式太阳能聚光器网架缩模相似设计及数值模拟

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机械设计 2016年第7期33卷 71-77页

作者：马俊彭佑多颜健余佳焕湖南科技大学机械设备健康维护省重点实验室湖南湘潭411201

开展了25 k W大型碟式太阳能聚光器网架缩比模型相似准则分析并进行了相似设计,根据网架原型与缩比模型的静动力相似参数,建立了原型及缩比网架的有限元模型,通过对原型和缩比网架的力学特性数值模拟,揭示了不同工况下二者在位移、应力... 详细信息

开展了25 k W大型碟式太阳能聚光器网架缩比模型相似准则分析并进行了相似设计,根据网架原型与缩比模型的静动力相似参数,建立了原型及缩比网架的有限元模型,通过对原型和缩比网架的力学特性数值模拟,揭示了不同工况下二者在位移、应力及自振特性方面的相似关系。结果表明,不同工况下缩比模型与原型的数值模拟结果误差均小于5%,二者在位移和应力分布及各阶振型上基本一致;网架基频频率相似误差较大,为进一步降低基频相似误差,缩比模型应增加构件侧向弯曲刚度,所作研究能够为后续网架模型的精确制作与模型试验提供参考。

关键词：碟式网架数值模拟缩比模型静动力特性

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The miR-21-5p enriched in the apoptotic bodies of M2 macrophage-derived extracellular vesicles alleviates osteoarthritis by changing macrophage phenotype

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Genes & Diseases 2023年第3期10卷 1114-1129页

作者： Leilei Qin Jianye yang Xudong Su Xilan li Yiting Lei Lili Dong Hong Chen Cheng Chen Chen Zhao huan Zhang Jun Deng Ning Hu Wei huang Department of Orthopaedics The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Orthopedic Laboratory of Chongqing Medical University The First Affiliated Hospital of Chongqing Medical UniversityChongqing 400016China Institute of Burn Research Southwest HospitalState Key Lab of TraumaBurn and Combined InjuryArmy Medical UniversityChongqing 400038China

Macrophages (Mφs) play a crucial role in the pathological progression of osteoarthritis (OA) by regulating inflammation and tissue repair. Decreasing pro-inflammatory M1-Mφs and increasing anti-inflammatory M2-Mφs can alleviate OA-related inflammation and promote cartilage repair. Apoptosis is a natural process associated with tissue repair. A large number of apoptotic bodies (ABs), a type of extracellular vesicle, are produced during apoptosis, and this is associated with a reduction in inflammation. However, the functions of apoptotic bodies remain largely unknown. In this study, we investigated the role of M2-Mφs-derived apoptotic bodies (M2-ABs) in regulating the M1/M2 balance of macrophages in a mouse model of OA. Our data show that M2-ABs can be targeted for uptake by M1-Mφs, and this reprograms M1-to-M2 phenotypes within 24 h. The M2-ABs significantly ameliorated the severity of OA, alleviated the M1-mediated pro-inflammatory environment, and inhibited chondrocyte apoptosis in mice. RNA-seq revealed that M2-ABs were enriched with miR-21–5p, a microRNA that is negatively correlated with articular cartilage degeneration. Inhibiting the function of miR-21–5p in M1-Mφs significantly reduced M2-ABs-guided M1-to-M2 reprogramming following in vitro cell transfection. Together, these results suggest that M2-derived apoptotic bodies can prevent articular cartilage damage and improve gait abnormalities in OA mice by reversing the inflammatory response caused by M1 macrophages. The mechanism underlying these findings may be related to miR-21-5p-regulated inhibition of inflammatory factors. The application of M2-ABs may represent a novel cell therapy, and could provide a valuable strategy for the treatment of OA and/or chronic inflammation.

关键词： Apoptotic body Extracellular vesicles Macrophage phenotype switch Micr oRNA-21 Osteoarthritis

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Adjuvant sorafenib after heptectomy for Barcelona Clinic Liver Cancer-stage C hepatocellular carcinoma patients

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World Journal of Gastroenterology 2016年第23期22卷 5384-5392页

作者： Feng Xia Li-Li Wu Wan-Yee Lau Hong-Bo huan Xu-Dong Wen Kuan-Sheng Ma Xiao-Wu Li Ping Bie Institute of Hepatobiliary Surgery Southwest HospitalSouthwest Cancer CenterThird Military Medical University Faculty of Medicine The Chinese University of Hong Kong

AIM: To investigate the efficacy and safety of adjuvant sorafenib after curative resection for patients with Barcelona Clinic Liver Cancer(BCLC)-stage C hepatocellular carcinoma(HCC).METHODS: Thirty-four HCC patients, classified as BCLC-stage C, received adjuvant sorafenib for highrisk of tumor recurrence after curative hepatectomy at a tertiary care university hospital. The study group was compared with a case-matched control group of 68 patients who received curative hepatectomy for HCC during the study period in a 1:2 ***: The tumor recurrence rate was markedly lower in the sorafenib group(15/34, 44.1%) than in the control group(51/68, 75%, P = 0.002). The median disease-free survival was 12 mo in the study group and 10 mo in the control group. Tumor number more than 3, macrovascular invasion, hilar lymph nodes metastasis, and treatment with sorafenib were significant factors of disease-free survival by univariate analysis. Tumor number more than 3 and treatment with sorafenib were significant risk factors of diseasefree survival by multivariate analysis in the Cox proportional hazards model. The disease-free survival and cumulative overall survival in the study group were significantly better than in the control group(P = 0.034 and 0.016, respectively). CONCLUSION: Our study verifies the potential benefit and safety of adjuvant sorafenib for both decreasing HCC recurrence and extending disease-free and overall survival rates for patients with BCLC-stage C HCC after curative resection.

关键词： Hepatectomy Hepatocellular carcinoma Sorafenib Survival Tumor recurrence

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地方政府与外商直接投资规模:一个三方博弈的解释

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世界经济研究 2007年第7期 15-20,81页

作者：张欢北京师范大学社会发展与公共政策研究所

随着中国利用外商直接投资规模的不断扩大,有关FDI规模是否适度的问题就值得关注。本文利用缺口理论,通过对中央政府、地方政府和外商直接投资者三方的博弈分析,探讨了FDI规模问题,以此解释了中国改革开放以来利用FDI规模增长的内在机制... 详细信息

随着中国利用外商直接投资规模的不断扩大,有关FDI规模是否适度的问题就值得关注。本文利用缺口理论,通过对中央政府、地方政府和外商直接投资者三方的博弈分析,探讨了FDI规模问题,以此解释了中国改革开放以来利用FDI规模增长的内在机制,并得出FDI规模问题实质是中央政府和地方政府间关系的结论。相应地,解决FDI规模问题就需要中央政府清理整顿地方政府与中央政策不一致的招商优惠政策。

关键词：外商直接投资规模地方政府

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Mechanism of CO_(2) enhanced oil recovery in shale reservoirs

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Petroleum Science 2021年第6期18卷 1788-1796页

作者： Hai-Bo Li Zheng-Ming yang Rui-Shan Li Ti-Yao Zhou He-Kun Guo Xue-Wei Liu Yi-Xin Dai Zhen-Guo Hu huan Meng PetroChina Research Institute of Petroleum Exploration and Development Beijing100083PR China Institute of Flow and Fluid Mechanics Chinese Academy of SciencesLangfang065007HebeiPR China Sinopec Jianghan Oilfield Company Research Institute of Exploration and Development Wuhan430223HubeiPR China University of Chinese Academy of Sciences Beijing100049PR China

Combined with NMR,core experiment,slim-tube tests,nano-CT and oil composition analysis,the mechanism of CO(2) enhanced oil recovery had been ***_(2) flooding under supercritical state could achieve higher oil *** the process of crude oil displaced by supercritical CO2,the average oil recovery was 46.98% at low displacement pressures and 73.35% at high displacement *** permeability of cores after CO2 flooding was only 28%-64% of those before *** to the expelled oil,the contents of asphaltenes and non-hydrocarbons decreased,and saturated hydrocarbons of above C25 were absent in some samples,indicating that they had been retained in cores as demonstrated by CT and NMR *** slim-tube tests,the heavy components of oil were expelled when the pressure increased to 30 *** was a reasonable bottom hole pressure(BHP) below which the heavy components driven out from the far-well zone would deposit in the near-well zone,and when the pressure was too high,the nonhydrocarbon detention may cause *** smaller throat and worse physical properties the porous media had,the higher displacement pressure would be required to achieve a good oil displacement *** increase in displacement pressure or time of interaction between oil and CO2 could effectively enhance oil recovery.

关键词： Shale oil CO_(2) Supercritical pressure NMR Core physical simulation Slim-tube test Component analysis Nano-CT

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Climatology of Cloud-base Height from Long-term Radiosonde Measurements in China

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Advances in Atmospheric Sciences 2018年第2期35卷 158-168页

作者： Yong ZHANG Lejian ZHANG Jianping GUO Jinming FENG Lijuan CAO yang WANG Qing ZHOU Liangxu LI Bai LI Hui XU Lin LIU Ning AN huan LIU Meteorological Observation Center China Meteorological Administration State Key Laboratory of Severe Weather Chinese Academy of Meteorological Sciences Key Laboratory of Regional Climate-Environment for Temperate East Asia Institute of Atmospheric PhysicsChinese Academy of Sciences National Meteorological Information Center China Meteorological Administration College of Global Change and Earth System Science Beijing Normal University

Clouds are critical to the global radiation budget and hydrological cycle, but knowledge is still poor concerning the observed climatology of cloud-base height （CBH） in China. Based on fine-resolution sounding observations from the China Radiosonde Network （CRN）, the method used to estimate CBH was modified, and uncertainty analyses indicated that the CBH is good enough. The accuracy of CBH estimation is verified by the comparison between the sounding-derived CBHs and those estimated from the micro-pulse lidar and millimeter-wave cloud radar. As such, the CBH climatology was compiled for the period 2006-16. Overall, the CBH exhibits large geographic variability across China, at both 0800 Local Standard Time （LST） and 2000 LST, irrespective of season. In addition, the summertime cloud base tends to be elevated to higher altitudes in dry regions [i.e., Inner Mongolia and the North China Plain （NCP）]. By comparison, the Tibetan Plateau （TP）, Pearl River Delta （PRD） and Sichuan Basin （SCB） have relatively low CBHs （〈 2.4 km above ground level）. In terms of seasonality, the CBH reaches its maximum in summer and minimum in winter. A low cloud base tends to occur frequently （〉 70%） over the TP, PRD and SCB. In contrast, at most sites over the yangtze River Delta （YRD） and the NCP, about half the cloud belongs to the high-cloud category. The CBH does not exhibit marked diurnal variation in summer, throughout all CRN sites, probably due to the persistent cloud coverage caused by the East Asia Summer Monsson. To the best of our knowledge, this is the first CBH climatology produced from sounding measurements in China, and provides a useful reference for obtaining observational cloud base information.

关键词： cloud base height radiosonde relative humidity China climatology

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有效介质理论离子导电模型的修正

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中南大学学报（自然科学版） 2007年第2期38卷 297-302页

作者：潘春跃张倩戴潇燕高金环中南大学化学化工学院湖南长沙410083

针对现有有效介质理论(EMT)导电模型存在的问题和不足,通过对复合固态聚合物电解质(CSPE)导电性增强机制的分析,引入新的无机填料界面层离子导电模型及模型参数,对EMT离子导电模型进行修正。采用原位复合法制备聚环氧乙烷(PEO)基聚合物... 详细信息

针对现有有效介质理论(EMT)导电模型存在的问题和不足,通过对复合固态聚合物电解质(CSPE)导电性增强机制的分析,引入新的无机填料界面层离子导电模型及模型参数,对EMT离子导电模型进行修正。采用原位复合法制备聚环氧乙烷(PEO)基聚合物电解质,通过实验测定样品离子电导率与无机填料SiO2含量之间的关系,应用现有的EMT离子导电模型和修正后的EMT离子导电模型分别对样品的离子电导率与无机填料含量关系进行计算,将计算数值与实验数值进行比较。结果表明,修正后的EMT离子导电模型的计算值与实验值吻合较好。

关键词：复合固态聚合物电解质有效介质理论离子导电模型

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石墨烯/银纳米复合材料的制备及其影响因素研究

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物理学报 2013年第14期62卷 482-487页

作者：范冰冰郭焕焕李稳贾瑜张锐郑州大学材料科学与工程学院郑州450001 郑州大学物理工程学院郑州450001 郑州航空工业管理学院郑州450053

以硝酸银、鳞片石墨为原料,在强碱环境下,制备得到石墨烯/银纳米复合材料,采用X射线衍射、红外吸收光谱、透射电子显微镜、紫外可见分光光度计对所制备的石墨烯/银纳米复合材料进行了表征.结果表明:氧化石墨烯和银离子在强碱NaOH的作用... 详细信息

以硝酸银、鳞片石墨为原料,在强碱环境下,制备得到石墨烯/银纳米复合材料,采用X射线衍射、红外吸收光谱、透射电子显微镜、紫外可见分光光度计对所制备的石墨烯/银纳米复合材料进行了表征.结果表明:氧化石墨烯和银离子在强碱NaOH的作用下,氧化石墨烯失去部分含氧官能团,被部分还原为石墨烯(rGO),银离子被还原为纳米银颗粒,均匀分布在氧化石墨烯片层表面,颗粒大小和分布受硝酸银用量、反应温度、NaOH的加入顺序及前驱物混合方式等因素影响,在GO与Ag粒子质量比为1:1.08时,负载在石墨烯片层上的银纳米颗粒集中在12nm左右.

关键词：石墨烯银纳米复合材料强碱溶液

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