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Journal of Integrative Plant Biology 2008年第3期50卷 329-337页

作者： Bin-shuang pang Xue-Yong Zhang Key Laboratory of Crop Germplasm and Biotechnology Ministry of Agriculture Institute of Crop Sciences Chinese Academy of Agricultural Sciences Beijing 100081 China

The high molecular weight glutenin subunit （HMW-GS） pair 1Bx13＋1By16 are recognized to positively correlate with bread-making quality; however, their molecular data remain unknown. In order to reveal the mechanism by which 1By16 and 1Bx13 creates high quality, their open reading frames （ORFs） were amplified from common wheat Atlas66 and Jimai 20 using primers that were designed based on published sequences of HMW glutenin genes. The ORF of 1By16 was 2220bp, deduced into 738 amino acid residues with seven cysteines including 59 hexapeptides and 22 nanopeptides motifs. The ORF of 1Bx13 was 2385bp, deduced into 795 amino acid residues with four cysteines including 68 hexapeptides, 25 nanopeptides and six tripepUdes motifs. We found that 1By16 was the largest y-type HMW glutenin gene described to date in common wheat. The 1By16 had 36 amino acid residues inserted in the central repetitive domain compared with 1By15. Expression in bacteria and western-blot tests confirmed that the sequence cloned was the ORF of HMW-GS 1By16, and that 1Bx13 was one of the largest 1Bx genes that have been described so far in common wheat, exhibiting a hexapeptide （PGQGQQ） insertion in the end of central repetitive domain compared with 1Bx7. A phylogenetic tree based on the deduced full-length amino acid sequence alignment of the published HMW-GS genes showed that the 1By16 was clustered with Glu-1B-2, and that the 1Bx13 was clustered with Glu-1B-1 alleles.

关键词： bacterial expression high molecular weight glutenin subunit 1Bx13 1By16 western-blot wheat.

Scaled radar cross section measurement method for lossy targets via dynamically matching reflection coefficients in THz band

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Chinese Physics B 2022年第6期31卷 759-764页

作者： shuang pang Yang Zeng Qi Yang Bin Deng Hong-Qiang Wang College of Electronic Science and Technology National University of Defense TechnologyChangsha 410073China

In the terahertz band,the dispersive characteristic of dielectric material is one of the major problems in the scaled radar cross section(RCS)measurement,which is inconsistent with the electrodynamics similitude deducted according to the Maxwell’s *** on the high-frequency estimation method of physical optics(PO),a scaled RCS measurement method for lossy objects is proposed through dynamically matching the reflection coefficients according to the distribution of the object *** of the model of SLICY are conducted,and the inversed RCS of the lossy prototype is obtained using the proposed *** the inversed RCS with the calculated results,the validity of the proposed method is *** proposed method provides an effective solution to the scaled RCS measurement for lossy objects in the THz band.

关键词： scaled measurement method radar cross section lossy targets terahertz

Disturbance-level-dependent post-disturbance succession in a Eurasian steppe

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Science China(Life Sciences) 2022年第1期65卷 142-150页

作者： Junjie Yang Minjie Xu shuang pang Lili Gao Zijia Zhang Zhiping Wang Yunhai Zhang Xingguo Han Ximei Zhang State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China Key Laboratory of Dryland Agriculture Ministry of AgricultureInstitute of Environment and Sustainable Development in AgricultureChinese Academy of Agricultural SciencesBeijing 100081China

Anthropogenic disturbances may decrease as we take measures to control ***,the patterns and mechanisms of postdisturbance ecosystem succession have rarely been *** we reported that disturbance level determined the importance of stochastic relative to deterministic changes in ecosystem components(plant community composition,soil microbial community composition,and soil physicochemical indices),and thus predefined the pattern of post-disturbance ecosystem *** proposed a theoretical framework with five disturbance levels corresponding to distinct succession *** conducted a nitrogen addition experiment in a temperate steppe,monitored these ecosystem components during"disturbance"treatment(2010-2014)and post-treatment"succession"(2014-2018).The disturbance level experienced by each component in each treatment was inferred by fitting the observed succession patterns into the theoretical *** mean disturbance level of these components was found to increase quadratically with nitrogen addition *** was because increasing nitrogen addition reduced the importance of stochastic relative to deterministic changes in these components,and these changes had a quadratic relationship with disturbance ***,our results suggested that by monitoring the importance of stochastic relative to deterministic changes in an ecosystem,we can estimate disturbance levels and predict succession patterns,as well as propose disturbance-level-dependent strategies for post-disturbance restoration.

关键词： disturbance grassland microbial diversity nitrogen deposition restoration stochastic process succession

Nitrogen deposition mediates more stochastic processes in structuring plant community than soil microbial community in the Eurasian steppe

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Science China(Life Sciences) 2024年第4期67卷 778-788页

作者： Wei Yang Shuhan Zhang Ang Li Junjie Yang shuang pang Zonghao Hu Zhiping Wang Xingguo Han Ximei Zhang Key Laboratory of Dryland Agriculture Ministry of AgricultureInstitute of Environment and Sustainable Development in AgricultureChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China

Anthropogenic environmental changes may affect community assembly through mediating both deterministic(e.g.,competitive exclusion and environmental filtering)and stochastic processes(e.g.,birth/death and dispersal/colonization).It is traditionally thought that environmental changes have a larger mediation effect on stochastic processes in structuring soil microbial community than aboveground plant community;however,this hypothesis remains largely *** we report an unexpected pattern that nitrogen(N)deposition has a larger mediation effect on stochastic processes in structuring plant community than soil microbial community(those<2 mm in diameter,including archaea,bacteria,fungi,and protists)in the Eurasian *** performed a ten-year nitrogen deposition experiment in a semiarid grassland ecosystem in Inner Mongolia,manipulating nine rates(0–50 g N m^(-2)per year)at two frequencies(nitrogen added twice or 12 times per year)under two grassland management strategies(fencing or mowing).We separated the compositional variation of plant and soil microbial communities caused by each treatment into the deterministic and stochastic components with a recently-developed *** nitrogen addition rate increased,the relative importance of stochastic component of plant community first increased and then decreased,while that of soil microbial community first decreased and then *** the whole,the relative importance of stochastic component was significantly larger in plant community(0.552±0.035;mean±standard error)than in microbial community(0.427±0.035).Consistently,the proportion of compositional variation explained by the deterministic soil and community indices was smaller for plant community(0.172–0.186)than microbial community(0.240–0.767).Meanwhile,as nitrogen addition rate increased,the linkage between plant and microbial community composition first became weaker and then became *** larger stochasticity in plant community relative to microbial

关键词： deterministic process environmental change grassland restoration nitrogen deposition stochastic process

Facile fabrication of branched-chain carbohydrate chips for studying carbohydrate-protein interactions by QCM biosensor

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Chinese Chemical Letters 2018年第1期29卷 65-68页

作者： Yuchao Lu Siyu Song Chenxi Hou shuang pang Xueming Li Xiaowen Wu Chen Shao Yuxin Pei Zhichao Pei Shaanxi Key Laboratory of Natural Products & Chemical Biology College of Chemistry and Pharmacy Northwest A & F University Attana

A novel approach for fabricating branched-chain （BC） carbohydrate chips to study carbohydrate-protein interactions using Quartz Crystal Microbalance （QCM） biosensor was developed. This approach utilizes functional alkynyl-branch molecule modified chip surfaces, which through the terminal alkynyl group for covalent linking of unprotected azide-carbohydrates. The unprotected azide-carbohydrates were syhthesized in one-step using 2-azido-l,B-dimethylqmidazolinium as catalyst, avoiding complex chemical modifications. Additionally, the branch surface modified with the carbohydrates not only supplies more specific binding site but also reveals significant cluster effect. To exemplify the sugar cluster effect on BC carbohydrate chips, BC Galactose and Mannose chips prepared in this work were used to determine carbohydrate-lectin interactions using QCM biosensor. The results clearly showed that BC chip significantly improves the detection sensitivity compared with the straight-chain （SC） chip. More importantly, the BC galactose chip sensitivity was enhanced 40% compared with the SC galactose chip.

关键词： MultivalentBrach carbohydrate chipO CMCarbohydrate-protein interactions

Tradeoff between productivity and stability across above-and below-ground communities

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Journal of Integrative Plant Biology 2024年第11期66卷 2321-2324页

作者： Zonghao Hu Haiyan Liu Junjie Yang Bin Hua Michael Bahn shuang pang Tingting Li Wei Yang Honghui Wu Xingguo Han Ximei Zhang State Key Laboratory of Efﬁcient Utilization of Agricultural Water Resources Institute of Environment and Sustainable Development in AgricultureChinese Academy of Agricultural SciencesBeijing 100081China State Key Laboratory of Vegetation and Environmental Change Institute of BotanyChinese Academy of SciencesBeijing 100093China College of Forestry Henan Agricultural UniversityZhengzhou 450002China Department of Ecology University of InnsbruckInnsbruck 6020Austria State Key Laboratory of Efﬁcient Utilization of Arid and Semi-arid Arable Land in Northern China Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China Key Laboratory of Arable Land Quality Monitoring and Evaluation Ministry of Agriculture and Rural AffairsInstitute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081China

Because of the aggravated agricultural fertilization and combustion of fossil fuels,the nitrogen(N)deposition rate has increased from the pre-industrial levels of about 0.1–0.3 to as high as 10 g N m^(-2)year^(-1)in some developed countries,and it is predicted to increase similarly over the next 50 years in many developing countries(Galloway et al.,2004).

关键词： Trade stability ground

类联接概念的演变及其与语料库语言学研究范式的内在联系

维普期刊数据库评论

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外语电化教学 2014年第2期 11-16页

作者：庞双子北京外国语大学中国外语教育研究中心北京100089 天津外国语大学英语学院天津300204

类联接概念自20世纪50年代初提出以来,从最初的词汇在"语法范畴间的共现关系"到2006年麦肯瑞(McEnery)将其列为词条为止,其内涵和外延逐渐扩大。本文从此概念提出的语言学史背景为起点进行分析,结合语料库语言学研究的两种范式(语料库... 详细信息

类联接概念自20世纪50年代初提出以来,从最初的词汇在"语法范畴间的共现关系"到2006年麦肯瑞(McEnery)将其列为词条为止,其内涵和外延逐渐扩大。本文从此概念提出的语言学史背景为起点进行分析,结合语料库语言学研究的两种范式(语料库驱动和基于语料库)及语料库技术的发展对类联接概念的演变进行总结和分析,从而得出结论:类联接概念的不断完善,正是这两种研究范式在对立中融合的体现。在将搭配研究推向更为抽象的层次的同时,类联接也充分反映了语言在各个层次的语法现象,从而将两种研究方式更加紧密地结合在一起。

关键词：类联接语料库驱动语料库语言学

“假说”和“原则”——沃尔夫语言相对论原则内涵解读

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外语学刊 2015年第1期 23-29页

作者：庞双子天津外国语大学天津300204 北京外国语大学北京100089

沃尔夫的语言相对论自提出以来,其名称不断演变,从沃尔夫假说到沃尔夫主义,又到新沃尔夫理论脉络,其间经历了3个阶段:验证、消沉和再认识。后人对于语言相对论褒贬不一,赞同者常被指责为浪漫主义、神秘主义;批判者经常被认为根本没有读... 详细信息

沃尔夫的语言相对论自提出以来,其名称不断演变,从沃尔夫假说到沃尔夫主义,又到新沃尔夫理论脉络,其间经历了3个阶段:验证、消沉和再认识。后人对于语言相对论褒贬不一,赞同者常被指责为浪漫主义、神秘主义;批判者经常被认为根本没有读过沃尔夫而将其简单粗俗化。本文通过对"假说"和"原则"的探源以及对于其内涵的系统化阐释的回顾,尝试分析沃尔夫语言相对论原则的内涵;从全面解读沃尔夫生前著述结集《论语言、思维和现实》入手,探求沃尔夫学说的本意:(1)通过对于语言范畴暗藏层面的比较,提高植根于语言内部文化属性的地位;(2)将科学与宗教结合起来,进一步探讨语言对于人类思维潜能的开发;(3)收集一手资料,进行一对一的实证尝试。

关键词：语言相对论原则沃尔夫假说思维

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翻译文本的语体特征——多维度语料库考察

上海翻译 2020年第6期 29-34页

作者：庞双子上海交通大学外国语学院上海200240

翻译研究的一个主要关注点就是源语经由翻译进入目标语言文化及其介入。通常翻译文本的考察多是集中在局部特征,缺乏语体层面多维度的考察分析。本研究通过历时复合语料库(约1000万字/词)考察近一百年间翻译文学语体特征的历史演变,并... 详细信息

翻译研究的一个主要关注点就是源语经由翻译进入目标语言文化及其介入。通常翻译文本的考察多是集中在局部特征,缺乏语体层面多维度的考察分析。本研究通过历时复合语料库(约1000万字/词)考察近一百年间翻译文学语体特征的历史演变,并分析翻译对于现代汉语语体发展的影响。研究发现,在反映语篇信息性的多项指标上,翻译文学语体的变化上呈现显著性差异,表现出信息严密化的发展趋势;而在指称明晰特征上,翻译文学语体表现出口语化发展趋势。

关键词：语料库语体演变翻译文本

双路混沌光注入的相位调制光反馈半导体激光器的混沌特性

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光学学报 2023年第3期43卷 156-165页

作者：庞爽冯玉玲于萍长春理工大学物理系吉林长春130022

混沌光的延时特征(TDS)和带宽(BW)是影响混沌激光应用的两个重要参量,常用来表征混沌光的混沌特性。将具有外腔光反馈的半导体激光器(SL)作为主激光器,将具有相位调制光反馈的SL作为从激光器,并将主激光器输出的混沌光单向双路注入到从... 详细信息

混沌光的延时特征(TDS)和带宽(BW)是影响混沌激光应用的两个重要参量,常用来表征混沌光的混沌特性。将具有外腔光反馈的半导体激光器(SL)作为主激光器,将具有相位调制光反馈的SL作为从激光器,并将主激光器输出的混沌光单向双路注入到从激光器中,构成具有外光单向双路注入的相位调制光反馈的SL系统。数值研究了外光注入系数和反馈系数等参数对系统输出混沌光TDS的影响,进而在TDS被有效抑制的参数条件下,对系统输出混沌光的带宽进行了研究。结果表明,用该方案和通过对参数值区间的适当选取可以有效地抑制混沌激光的TDS,并且混沌激光的3 dB带宽最大约为20 GHz。

关键词：激光器分布反馈半导体激光器混沌激光相位调制光反馈延时特征带宽