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Journal of Rare Earths 2022年第8期40卷 1255-1263页

作者： Xuejun Xu Tianhua Huang Yisheng Xu Huawen Hu shuang liao Xiaohong Hu Dongchu Chen Min Zhang School of Materials Science and Hydrogen Energy Foshan UniversityFoshan 528000China Guangdong Key Laboratory for Hydrogen Energy Technologies Foshan 528000China

CeO_(2)/g-C_(3)N_(4)photocatalysts have attracted tremendous attention in the photocatalytic degradation of organic pollutants.The design and construction of highly active CeO_(2)/g-C_(3)N_(4)photocatalysts without harsh conditions are still challenging.Herein,highly dispersed CeO_(2-x)nanoparticles with rich oxygen vacancies were successfully precipitated on the surface of g-C_(3)N_(4)under mild conditions.The fabricated CeO_(2-x)/g-C_(3)N_(4)exhibits remarkable activity and stability for photocatalytic degradation of MO pollutant.The optimal rate constant of MO degradation over CeO_(2-x)/g-C_(3)N_(4)is about 0.031 min^(-1),which is three times higher than that of g-C_(3)N_(4).A negligible activity decrease is observed after three cycling runs.The enhanced catalytic performance can be ascribed to the excellent dispersion of CeO_(2-x)with rich oxygen vacancies that benefit O_(2)adsorption and visible light absorption.In addition,the proper band alignment between CeO_(2-x)and gC_(3)N_(4)is conducive to the highly efficient separation of photogenerated electron-hole pairs.

关键词： CeO_(2-x) G-C_(3)N_(4) Heterojunctions Oxygen vacancies Rare earths

重庆外贸企业参与西部陆海新通道建设的现状、问题及对策

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西部学刊 2024年第1期 1-4页

作者：廖双重庆对外经贸学院外语外贸学院重庆401520

西部陆海新通道是重庆借助陆路和海运进行国际贸易和物流的新兴通道,它的建设对于新形势下重庆加强与世界的贸易联系和经济合作具有重要意义。经过六年发展,重庆外贸企业经新通道的交易在运输规模、产品多元化、密切联系东盟、业态创新... 详细信息

西部陆海新通道是重庆借助陆路和海运进行国际贸易和物流的新兴通道,它的建设对于新形势下重庆加强与世界的贸易联系和经济合作具有重要意义。经过六年发展,重庆外贸企业经新通道的交易在运输规模、产品多元化、密切联系东盟、业态创新等方面成效显著,但受到企业文化、组织结构、资源配置的内部制约,同时还面临物流成本、机制协同、服务配套、交通基建等外部环境方面的突出难题。解决这些问题,对企业而言,要营造开放、创新的学习型企业文化氛围,构建扁平、分权的客户导向型组织结构,形成保量、提质的数智型资源配置模式。同时,要完善灵活、合理的一站式物流运价体系,探索便捷、高效的标准化多式联运管理模式,打造专业、个性的高质量综合服务链,建设配套密集、畅通的国际级基础设施网络。

关键词：重庆外贸企业西部陆海新通道通道经济

Structural and functional characterization of multiple myeloma associated cytoplasmic poly(A) polymerase FAM46C

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Cancer Communications 2021年第7期41卷 615-630页

作者： Hong Zhang Shi-Hui Zhang Jia-Li Hu Yu-Tong Wu Xiao-Yan Ma Yang Chen Bing Yu shuang liao Huilin Huang Song Gao State Key Laboratory of Oncology in South China Collaborative Innovation Center for Cancer MedicineSun Yat-sen University Cancer CenterGuangzhouGuangdong 510060P.R.China Department of Oncology The Second Affiliated Hospital of Nanchang UniversityNanchangJiangxi 330006P.R.China Guangzhou Regenerative Medicine and Health Guangdong Laboratory GuangzhouGuangdong 510530P.R.China

Background:Multiple myeloma(MM)is a hematologic malignancy characterized by the accumulation of aberrant plasma cells within the bone marrow.The high frequent mutation of family with sequence similarity 46,member C(FAM46C)is closely related with the occurrence and progression of MM.Recently,FAM46C has been identified as a non-canonical poly(A)polymerase(PAP)that functions as a tumor suppressor in MM.This study aimed to elucidate the structural features of this novel non-canonical PAP and how MM-related mutations affect the structural and biochemical properties of FAM46C,eventually advancing our understandings towards FAM46C mutation-related MM occurrence.Methods:We purified and crystallized a mammalian FAM46C construct,and solved its structure.Next,we characterized the property of FAM46C as a PAP through a combination of structural analysis,site-directed mutagenesis and biochemical assays,and by comparison with its homolog FAM46B.Finally,we structurally analyzed MM-related FAM46C mutations and tested the enzymatic activity of corresponding mutants.Results:We determined the crystal structure of a mammalian FAM46C protein at 2.35 A,and confirmed that FAM46C preferentially consumed adenosine triphosphate(ATP)and extended A-rich RNA substrates.FAM46C showed a weaker PAP activity than its homolog FAM46B,and this difference was largely dependent on the residue variance at particular sites.Of them,residues at positions 77,290,and 298 of mouse FAM46C weremost important for the divergence in enzymatic activity.Among the MM-associated FAM46C mutants,those residing at the catalytic site(D90G and D90H)or putative RNA-binding site(I155L,S156F,D182Y,F184L,Y247V,andM270V)showed abolished or compromised PAP activity of FAM46C,while N72A and S248A did not severely affect the PAP activity.FAM46C mutants D90G,D90H,I155L,S156F,F184L,Y247V,and M270V had significantly lower inhibitory effect on apoptosis of RPMI-8226 cells as compared to wild-type FAM46C.Conclusions:FAM46C is a prokaryotic-like PAP with preference forA-richRNA substra

关键词： apoptosis crystal structure FAM46C miRNA multiple myeloma poly(A)polymerase PTEN

优质杂交水稻新组合绵两优2115高产制种技术

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杂交水稻 2024年第1期39卷 99-101页

作者：廖爽刘定友余华清项祖芬石军黄廷友绵阳市农业科学研究院作物特色资源创制及应用四川省重点实验室四川绵阳621023

绵两优2115是绵阳市农业科学研究院利用自主选育的优质两用核不育系绵13S与四川农业大学农学院选育的恢复系雅恢2115配组育成的优质两系杂交水稻新组合,2021年通过四川省农作物品种审定委员会审定。根据该组合亲本特性以及四川绵阳的气... 详细信息

绵两优2115是绵阳市农业科学研究院利用自主选育的优质两用核不育系绵13S与四川农业大学农学院选育的恢复系雅恢2115配组育成的优质两系杂交水稻新组合,2021年通过四川省农作物品种审定委员会审定。根据该组合亲本特性以及四川绵阳的气候特点,基于其2018—2020年在绵阳的制种生产实践,总结了其高产制种技术。

关键词：杂交水稻绵两优2115 制种

Role of CAST-Drp1 Pathway in Retinal Neuron-Regulated Necrosis in Experimental Glaucoma

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Current Medical Science 2023年第1期43卷 166-172页

作者： Su-mei LIU Lv-shuang liao Ju-fang HUANG Shu-chao WANG Department of Anesthesiology The Second Xiangya Hospital of Central South UniversityChangsha410011China Center for Medical Research The Second Xiangya Hospital of Central South UniversityChangsha410011China Department of Anatomy and Neurobiology School of Basic Medical SciencesCentral South UniversityChangsha410013China School of Physical Education Hunan Institute of Science and TechnologyYueyang430600China

Objective Numerous studies have indicated that excitatory amino acid toxicity,such as glutamate toxicity,is involved in glaucoma.In addition,excessive glutamate can lead to an intracellular calcium overload,resulting in regulated necrosis.Our previous studies have found that the calpastatin(CAST)-calpain pathway plays an important role in retinal neuron-regulated necrosis after glutamate injury.Although inhibition of the calpain pathway can decrease regulated necrosis,necrotic cells remain.It has been suggested that there are other molecules that participate in retinal neuron-regulated necrosis.CAST is an important regulator of dynamin-related protein 1(Drp1)-mediated mitochondrial defects.Thus,the aim of this study was to determine whether the CAST-Drp1 pathway may be an underlying signaling axis in neuron-regulated necrosis.Methods Using cultured retinal neurons and in an in-vivo glaucoma model induced by glutamate overload,members of the CAST-Drp1 pathway were assessed by immunofluorescence,Western blotting,Phos-tagTM SDS-PAGE,and co-immunoprecipitation assays.Moreover,the black and white box test was performed on the rats.Results We found that more retinal neuron-regulated necrosis and Drp1 activation as well as lower CAST levels were present in the glutamate-induced glaucoma model.Rats with glutamate-induced glaucoma exhibited impaired visual function.We also observed retinal neuron-regulated necrosis and Drp1 activity decreased,and impaired vision recovered after CAST active peptide application,indicating that the CAST-Drp1 pathway plays a critical role in retinal neuron-regulated necrosis and visual function.Conclusion The results of this study indicate that the CAST-Drp1 pathway protects against retinal neuron-regulated necrosis,which may expand the therapeutic targets for the treatment of neurodegenerative disorders involving dysfunction of glutamate metabolism,such as glaucoma.

关键词： retinal ganglion cells glaucoma calpastatin dynamin-related protein l central nervous system regulated necrosis glutamate

Do pyroptosis, apoptosis, and necroptosis (PANoptosis) exist in cerebral ischemia? Evidence from cell and rodent studies

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Neural Regeneration Research 2022年第8期17卷 1761-1768页

作者： Wei-Tao Yan Yan-Di Yang Xi-Min Hu Wen-Ya Ning Lyu-shuang liao shuang Lu Wen-Juan Zhao Qi Zhang Kun Xiong Department of Neurobiology and Human Anatomy School of Basic Medical ScienceCentral South UniversityChangshaHunan ProvinceChina Department of Dermatology Xiangya HospitalCentral South UniversityChangshaHunan ProvinceChina Department of Human Resources Third Xiangya Hospital of Central South UniversityChangshaHunan ProvinceChina Hunan Key Laboratory of Ophthalmology ChangshaHunan ProvinceChina

Some scholars have recently developed the concept of PANoptosis in the study of infectious diseases where pyroptosis,apoptosis and necroptosis act in consort in a multimeric protein complex,PANoptosome.This allows all the components of PANoptosis to be regulated simultaneously.PANoptosis provides a new way to study the regulation of cell death,in that different types of cell death may be regulated at the same time.To test whether PANoptosis exists in diseases other than infectious diseases,we chose cerebral ischemia/reperfusion injury as the research model,collected articles researching cerebral ischemia/reperfusion from three major databases,obtained the original research data from these articles by bibliometrics,data mining and other methods,then integrated and analyzed these data.We selected papers that investigated at least two of the components of PANoptosis to check its occurrence in ischemia/reperfusion.In the cell model simulating ischemic brain injury,pyroptosis,apoptosis and necroptosis occur together and this phenomenon exists widely in different passage cell lines or primary neurons.Pyroptosis,apoptosis and necroptosis also occurred in rat and mouse models of ischemia/reperfusion injury.This confirms that PANoptosis is observed in ischemic brain injury and indicates that PANoptosis can be a target in the regulation of various central nervous system diseases.

关键词： apoptosis brain central nervous system ischemia/reperfusion middle cerebral artery occlusion necroptosis oxygen and glucose deprivation PANoptosis pyroptosis regulated cell death

1例生痰二氧化碳嗜纤维菌脑膜炎合并肾功能亢进患儿的抗感染治疗实践

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儿科药学杂志 2024年第6期30卷 1-5页

作者：何翠瑶李听松罗媛媛廖双重庆医科大学附属儿童医院国家儿童健康与疾病临床医学研究中心儿童发育疾病研究教育部重点试验室儿科学重庆市重点实验室重庆400014

目的:探讨临床药师在儿童细菌性脑膜炎抗感染治疗中的作用。方法:临床药师通过参与1例由生痰二氧化碳嗜纤维菌引起的儿童细菌性脑膜炎患者的多次药学会诊,与医师通力合作,对患儿治疗效果欠佳的原因进行分析,结合患儿存在肾功能亢进这一... 详细信息

目的:探讨临床药师在儿童细菌性脑膜炎抗感染治疗中的作用。方法:临床药师通过参与1例由生痰二氧化碳嗜纤维菌引起的儿童细菌性脑膜炎患者的多次药学会诊,与医师通力合作,对患儿治疗效果欠佳的原因进行分析,结合患儿存在肾功能亢进这一特殊生理病理特点及抗菌药物药动学/药效学(PK/PD)理论,优化抗感染治疗方案并实施药学监护。结果:临床药师提出的治疗方案被医师采纳,患儿临床症状改善、脑脊液恢复正常并顺利出院。结论:临床药师对临床上罕见致病菌引起的特殊部位感染,应充分发挥药学专业特长,进一步提高抗感染药物的临床疗效。

关键词：生痰二氧化碳嗜纤维菌细菌性脑膜炎肾功能亢进临床药师

Base editor-mediated large-scale screening of functional mutations in bacteria for industrial phenotypes

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Science China(Life Sciences) 2024年第5期67卷 1051-1060页

作者： Yaomeng Yuan Xihao liao shuang Li Xin-Hui Xing Chong Zhang MOE Key Laboratory for Industrial Biocatalysis Institute of Biochemical EngineeringDepartment of Chemical EngineeringTsinghua UniversityBeijing 100084China Institute of Biopharmaceutical and Health Engineering Tsinghua Shenzhen International Graduate SchoolShenzhen 518055China Institute of Biomedical Health Technology and Engineering Shenzhen Bay LaboratoryShenzhen 440300China Center for Synthetic and Systems Biology Tsinghua UniversityBeijing 100084China

Base editing,the targeted introduction of point mutations into cellular DNA,holds promise for improving genome-scale functional genome screening to single-nucleotide resolution.Current efforts in prokaryotes,however,remain confined to loss-of-function screens using the premature stop codons-mediated gene inactivation library,which falls far short of fully releasing the potential of base editors.Here,we developed a base editor-mediated functional single nucleotide variant screening pipeline in Escherichia coli.We constructed a library with 31,123 sgRNAs targeting 462 stress response-related genes in E.coli,and screened for adaptive mutations under isobutanol and furfural selective conditions.Guided by the screening results,we successfully identified several known and novel functional mutations.Our pipeline might be expanded to the optimization of other phenotypes or the strain engineering in other microorganisms.

关键词： base editor high-throughput large-scale screening Escherichia coli functional mutation

Effect of aflibercept combined with triamcinolone acetonide on aqueous humor growth factor and inflammatory mediators in diabetic macular edema

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 297-303页

作者： De-shuang Li Hong-Xia liao Jing-Lin Zhang Bo Qin Shenzhen Aier Eye Hospital Aier Eye HospitalJinan UniversityShenzhen 518031Guangdong ProvinceChina Guangzhou Aier Eye Hospital Jinan UniversityGuangzhou 510040Guangdong ProvinceChina

AIM:To investigate the efficacy of aflibercept combined with sub-tenon injection of triamcinolone acetonide(TA)in treating diabetic macular edema(DME)and to examine changes in growth factors and inflammatory mediator levels in aqueous humor after injection.METHODS:Totally 67 DME patients(67 eyes)and 30 cataract patients(32 eyes)were enrolled as the DME group and the control group,respectively.The DME group was divided into the aflibercept group(34 cases)and the aflibercept combined with TA group(combined group,33 cases).The aqueous humor of both groups was collected during the study period.The aqueous levels of vascular endothelial growth factor(VEGF),monocyte chemoattractant protein-1(MCP-1),interleukin-6(IL-6),interleukin-8(IL-8),and interleukin-1β(IL-1β)were detected using a microsphere suspension array technology(Luminex 200TM).Aqueous cytokines,best-corrected visual acuity(BCVA),central macular thickness(CMT),and complications before and after treatment were compared between the aflibercept group and combined group.RESULTS:The concentrations of VEGF,MCP-1,IL-6,and IL-8 in the aqueous humor were significantly higher in the DME group than those of the control group(all P0.05).CONCLUSION:The cytokines VEGF,MCP-1,IL-6,and IL-8 in the aqueous humor of DME patients are significantly increased.Aflibercept and aflibercept combined with TA have good efficacy in DME patients,can effectively reduce CMT,improve the patient’s vision,and have high safety.Aflibercept combined with TA can quickly downregulate the aqueous humor cytokines and help to relieve macular edema rapidly.However,the long-term efficacy is comparable to that of aflibercept alone

关键词： diabetic macular edema aqueous humor cytokines aflibercept triamcinolone acetonide

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天然橡胶与异戊橡胶支链结构对比分析

合成橡胶工业 2024年第1期47卷 50-52页

作者：李泽春齐兴国项健廖双泉杨昌金浙江万马高分子材料集团有限公司杭州311305 海南大学材料与化工学院/热带岛屿资源先进材料教育部重点实验室海口570228

使用核磁共振波谱仪对比分析了进口天然橡胶RSS-1与异戊橡胶分子链的支链结构。结果显示,RSS-1分子链的支链结构中环氧基团的摩尔分数为0.02%,羟基为0.16%,3,4-异戊二烯单元为0.20%;异戊橡胶的分子链支链结构中不含有环氧基团和羟基,3,4... 详细信息

使用核磁共振波谱仪对比分析了进口天然橡胶RSS-1与异戊橡胶分子链的支链结构。结果显示,RSS-1分子链的支链结构中环氧基团的摩尔分数为0.02%,羟基为0.16%,3,4-异戊二烯单元为0.20%;异戊橡胶的分子链支链结构中不含有环氧基团和羟基,3,4-异戊二烯单元的摩尔分数为0.94%。

关键词：天然橡胶异戊橡胶核磁共振波谱支链结构