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Smyd1 is essential for myosin expression and sarcomere organization in craniofacial,extraocular,and cardiac muscles

Journal of Genetics and Genomics 2021年第3期48卷 208-218页

作者： shuang jiao Rui Xu Shaojun Du Department of Biochemistry and Molecular Biology Institute of Marine and Environmental TechnologyUniversity of Maryland School of MedicineBaltimoreMD 21202USA Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China School of Life Sciences Shandong University of TechnologyZibo 255049China

Skeletal and cardiac muscles are striated myofibers that contain highly organized sarcomeres for muscle *** studies revealed that Smyd1,a lysine methyltransferase,plays a key role in sarcomere assembly in heart and trunk skeletal ***,Smyd1 expression and function in craniofacial muscles are not ***,we analyze the developmental expression and function of two smyd1 paralogous genes,smyd1 a and smyd1 b,in craniofacial and cardiac muscles of zebrafish *** data show that loss of smyd1a(smyd1a^(mb5))or smyd1b(smyd1b^(sa15678))has no visible effects on myogenic commitment and expression of myod and myosin heavy-chain m RNA transcripts in craniofacial ***,myosin heavy-chain protein accumulation and sarcomere organization are dramatically reduced in smyd1b^(sa15678) single mutant,and almost completely diminish in smyd1a^(mb5);smyd1b^(sa15678) double mutant,but not in smyd1a^(mb5) *** defects are also observed in cardiac muscles of smyd1b^(sa15678) *** craniofacial and cardiac muscle formation is associated with an upregulation of hsp90α1 and unc45b mRNA expression in smyd1b^(sa15678) and smyd1a^(mb5);smyd1b^(sa15678) ***,our studies indicate that Smyd1b,but not Smyd1a,plays a key role in myosin heavy-chain protein expression and sarcomere organization in craniofacial and cardiac *** of smyd1b results in muscle-specific stress response.

关键词： Smyd1 Myosin Sarcomere Craniofacial muscle Cardiac muscle

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Multiple Isoforms of Olive Flounder (Paralichthys olivaceus) Pax3a and Pax3b Display Differential Regulations on Myogenic Differentiation

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Journal of Ocean University of China 2022年第5期21卷 1295-1306页

作者： jiao shuang TAN Xungang YOU Feng PANG Qiuxiang Anti-Aging&Regenerative Medicine Research Institution School of Life Sciences and MedicineShandong University of TechnologyZibo 255000China Laboratory of Developmental and Evolutionary Biology School of Life Sciences and MedicineShandong University of TechnologyZibo 255000China CAS and Shandong Province Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Paired box 3(Pax3)is a critical upstream regulator of the onset of *** have previously identified two spliced isoforms of pax3a(pax3a-1 and pax3a-2)and three spliced isoforms of pax3b(pax3b-1,pax3b-2,and pax3b-3)in olive flounder,but their roles in myogenesis are *** this study,we investigated their cellular localization,transcriptional activity on myod gene regulation,and roles in *** Pax3a and Pax3b isoforms revealed various subcellular localizations,which were related to their corresponding protein ***3a-1,Pax3a-2,and Pax3b-1 promoted the transcriptional activity of myod to dif-ferent degrees,whereas Pax3b-2 and Pax3b-3 had a slight inhibitory or no *** pairwise interaction analysis demonstrated the synergistic effect of Pax3b-1 and Pax3b-3 on myod transcriptional *** overexpression of different pax3a and pax3b isoforms differentially altered the spatial expression patterns of myod and differentially regulated the expression levels of their target genes(myod,myf5,and c-met)in zebrafish embryonic *** addition,the different flounder myod promoter-driven pax3a/3b isoform expression vectors were successfully introduced into the skeletal muscles of juvenile flounder by ***-ever,none of them could change the mRNA expression levels of mstn,myf5,myod,myogenin,pax7a,and pax7b in the electroporated *** results suggest that different Pax3a and Pax3b isoforms may precisely and collaboratively regulate embryonic myogenesis,but their roles in juvenile myogenesis are uncertain.

关键词： Pax3a Pax3b isoforms myod myogenesis

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Recyclable adsorbent of BiFeO_3/Carbon for purifying industrial dye wastewater via photocatalytic reproducible

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Green Energy & Environment 2019年第1期4卷 66-74页

作者： shuang jiao Yiming Zhao Chensha Li Binsong Wang Yang Qu Key Laboratory of Functional Inorganic Materials Chemistry (Heilongjiang University) Ministry of Education School of Chemistry and Materials ScienceInternational Joint Research Center for Catalytic Technology Key Laboratory of Functional Inorganic Materials Chemistry(Heilongjiang University) Ministry of Education School of Chemistry and Materials ScienceInternational Joint Research Center for Catalytic Technology

It is essential to prepare highly-efficiency reproducible adsorbent for purifying industrial dye wastewater. In this work, biscuit with a layered porous structure as a template is applied to prepare a photocatalytic recyclable adsorbent of BiFeO3/Carbon nanocomposites for purifying simulative industrial dye wastewater. It is found that the structure of the prepared BiFeO3/Carbon nanocomposite is related to the natural structure of the biscuit, annealing temperatures and immersing times, demonstrated by XRD, TEM, UV-Vis and adsorptive activities. Kinetics data shows that the adsorption rate of the adsorbent to the dye is rapid and fitted well with the pseudo-second-order model, that more than 80% of dyes can be removed in the beginning 30 min. The adsorption isotherm can be perfectly described by the Langmuir model as well. It can be seen from the adsorption data that the adsorption performance can reach over 90% at pH ? 2–12, which can imply its universal utilization. The prepared BiFeO_3/Carbon nanocomposites have also displayed excellent capacities(over 90% within 30 min) for adsorption of seven different dyes and their mixed one. According to the five times photocatalytic reproducible experiments, it is proved that BiFeO_3/Carbon nanocomposites show the excellent stability and reproduction for purifying simulative industrial dyes, even the sample have been placed for one year. These research results indicate that the adsorbent BiFeO_3/Carbon can be a suitable material used in treating industrial dye wastewater potentially.

关键词： Reproducible adsorbent BiFeO3/Carbon nanocomposites Photocatalysis Purifying industrial dye wastewater Pseudo-second-order model

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Combined effects of electroacupuncture and anti-depression drugs on the hippocampus and frontal lobe

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Neural Regeneration Research 2010年第22期5卷 1723-1727页

作者： Dongmei Duan Ya Tu shuang jiao Liping Chen Department of TCM of Nan Lou Chinese PLA General Hospital Beijing 100853 China School of Acupuncture and Moxibustion Beijing University of Chinese Medicine Beijing 100029 China

Electroacupuncture （EA） has been clinically used to treat depression and has resulted in favorable effects in China. However, results from animal studies and pathology do not reflect the influence of electroacupuncture treatment on in vivo physiological functions. To thoroughly and dynamically observe pathological changes during depression, the present study established EA ＋ fluoxetine and fluoxetine groups to observe depression in patients. 1H-magnetic resonance spectroscopy was utilized to determine the correlation between hippocampal frontal lobe metabolite changes and mental disorder scale. Results revealed significantly increased N-acetylaspartate （NAA）/creatine （Cr） in the bilateral hippocampus and right frontal lobe of depression patients treated with EA compared with fluoxetine. Changes in NAA/Cr in bilateral hippocampus and right frontal lobe in both groups, before and after treatment, negatively correlated with severity and curative effects. Choline/Cr changes in the bilateral frontal lobes of both groups were significant before and after treatment, but negatively correlated with curative effects. Choline/Cr changes in the bilateral hippocampus were significant in the EA ＋ fluoxetine group before and after treatment, but negatively correlated with severity and the curative effects of depression. These results demonstrate abnormal biochemical metabolism in bilateral frontal lobes and hippocampus of depression patients, and show that EA significantly altered biochemical indices in the frontal lobes and hippocampus compared with fluoxetine.

关键词： depression electroacupuncture fluoxetine hippocampus frontal lobe traditional Chinese medicine magnetic resonance spectroscopy neural regeneration

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Antidepressant effect of electroacupuncture regulates signal targeting in the brain and increases brain- derived neurotrophic factor levels

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Neural Regeneration Research 2016年第10期11卷 1595-1602页

作者： Dong-mei Duan Ya Tu Ping Liu shuang jiao Department of Health Care of South Building Chinese PLA General Hospital Beijing China Acupuncture College of Beijing University of Chinese Medicine Beijing China Department of Clinical Pharmacology Chinese PLA General Hospital Beijing China Journal of Traditional Chinese Medicine Beijing China

Electroacupuncture improves depressive behavior faster and with fewer adverse effects than antidepressant medication. However, the antidepressant mechanism of electroacupuncture remains poorly understood. Here, we established a rat model of chronic unpredicted mild stress, and then treated these rats with electroacupuncture at Yintang （EX-HN3） and Baihui （DU20） with sparse waves at 2 Hz and 0.6 mA for 30 minutes, once a day. We found increased horizontal and vertical activity, and decreased immobility time, at 2 and 4 weeks after treatment. Moreover, levels of neurotransmitters （5-hydroxytryptamine, glutamate, and y-aminobutyric acid） and protein levels of brain-derived neurotrophic factor and brain-derived neurotrophic factor-related proteins （TrkB, protein kinase A, and phosphorylation of cyclic adenosine monophosphate response element binding protein） were increased in the hippocampus. Similarly, protein kinase A and TrkB mRNA levels were increased, and calcium-calmodulin-dependent protein kinase lI levels decreased. These findings suggest that electroacupuncture increases phosphorylation of cyclic adenosine monophosphate response element binding protein and brain-derived neurotrophic factor levels by regulating multiple targets in the cyclic adenosine rnonophosphate response element binding protein signal- ing pathway, thereby promoting nerve regeneration, and exerting an antidepressive effect.

关键词： nerve regeneration depression chronic unexpected mild stress electroacupuncture brain-derived neurotrophic factor neurotransmitter cyclic adenosine monophosphate response element binding protein signal pathway protein kinase A TrKB fluoxetine neural regeneration

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Molecular identification,expression pattern,and in-vitro bioactivity analysis of insulin-like growth factor 2 in olive flounder Paralichthys olivaceus

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Journal of Oceanology and Limnology 2023年第1期41卷 280-289页

作者： shuang jiao Xungang TAN Feng YOU Shujing ZHANG Qiuxiang PANG Anti-Aging&Regenerative Medicine Research Institution School of Life Sciences and MedicineShandong University of TechnologyZibo 255000China CAS and Shandong Province Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Insulin-like growth factors(IGFs)are key regulators of development and ***,we characterized the igf2 gene from olive flounder(Paralichthys olivaceus)and determined its temporal and spatial *** set up an in-vitro protein expression system in eukaryotic human embryonic kidney(HEK293 T)cells and explored its effects on cell *** flounder igf2 cDNA contained a 648-bp open reading frame(ORF)encoding a protein of 215 amino acids(aa),which spanned the complete signal peptide(47 aa),mature peptide(70 aa),and E domain(98 aa).In adult flounder,igf2 mRNA was detected in all selected *** early development,igf2 mRNA was detected throughout development from unfertilized eggs to hatching-stage ***-situ hybridization analysis indicated that igf2 mRNA was specially expressed in the brain region,floor plate,hypochord,otic vesicle,and pectoral fin during *** blotting analysis indicated that the soluble recombinant flounder IGF2 protein was successfully produced through eukaryotic expression in HEK293T *** addition,the recombinant IGF2 protein significantly promoted the proliferation of human cervical carcinoma(HeLa)and HEK293T *** results provide new information about the structural and functional conservation,expression patterns,and biological activity of the igf2 in teleosts.

关键词： aquaculture flatfish igf2 proliferation

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Genome Editing in the Olive Flounder(Paralichthys olivaceus)Using CRISPR/Cas9 and a Simple Microinjection System

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Journal of Ocean University of China 2021年第6期20卷 1528-1536页

作者： TAN Xungang WANG Ling WU Zhihao jiao shuang WANG Lijuan ZOU Yuxia JIANG Jingteng YOU Feng CAS and Shandong Province Key Laboratory of Experimental Marine Biology Center for Ocean Mega-ScienceInstitute of OceanologyChinese Academy of SciencesQingdao 266071China Laboratory for Marine Biology and Biotechnology Qingdao National Laboratory for Marine Science and TechnologyQingdao 266071China University of Chinese Academy of Sciences Beijing 100049China Weihai Fishery Technology Promotion Station Weihai 264200China Shenghang Aquatic Science and Technology Co. Ltd.Weihai 264200China

The whole-genome sequence of the olive flounder(Paralichthys olivaceus)provides a basis for gene functional analyses,which is important for the aquaculture *** gene function will help us to select better economic traits such as fast growth and better culture conditions,which further will increase the aquaculture *** knockout is an important reverse genetics approach for in vivo studies of gene *** this study,the CRISPR/Cas9 genome editing method with a microinjection system using a simple braked needle was employed in olive *** injection in embryos,green fluorescent protein expression was detected in 40%of *** proportion of normal-hatched larvae was approximately 50%.Different mutations,including short indels and fragment deletions,were found in our test genes gsdf and ***,we detected more than one mutation in a single *** summary,our microinjection technique and CRISPR/Cas9 can be applied to study gene functions in olive flounder.

关键词： CRISPR/Cas9 gsdf microinjection myomaker needle with brake olive flounder

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Influence of original microstructure on the transformation behavior and mechanical properties of ultra-high-strength TRIP-aided steel

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International Journal of Minerals,Metallurgy and Materials 2015年第3期22卷 262-271页

作者： Hong-xiang Yin Ai-min Zhao Zheng-zhi Zhao Xiao Li shuang-jiao Li Han-jiang Hu Wei-guang Xia Metallurgical Engineering Research Institute University of Science and Technology Beijing Beijing Laboratory of Metallic Materials and Processing for Modern Transportation Key Laboratory for Mechanics in Fluid Solid Coupling Systems Institute of Mechanics Chinese Academy of Sciences

The transformation behavior and tensile properties of an ultra-high-strength transformation-induced plasticity （TRIP） steel （***） were investigated by different heat treatments for automobile applications. The results show that F-TRIP steel, a tradi- tional TRIP steel containing as-cold-rolled ferfite and pearlite as the original microstructure, consists of equiaxed grains of intercritical ferrite surrounded by discrete particles of M/RA and B. In contrast, M-TRIP steel, a modified TRiP-aided steel with martensite as the original mi- crostlucture, containing full martensite as the original microstructure is comprised of lath-shaped grains of ferrite separated by lath-shaped martensite/retained austenite and bainite. Most of the austenite in F-TRIP steel is granular, while the austenite in M-TRIP steel is lath-shaped. The volume fraction of the retained austenite as well as its carbon content is lower in F-TRIP steel than in M-TRIP steel, and austenite grains in M-TRIP steel are much finer than those in F-TRIP steel. Therefore, M-TRIP steel was concluded to have a higher austenite stability, re- sulting in a lower transformation rate and consequently contributing to a higher elongation compared to F-TRIP steel. Work hardening be- havior is also discussed for both types of steel.

关键词： high strength steels transformation-induced plasticity phase transformations mechanical properties original microstructure work hardening

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《中医杂志》打造媒体融合出版矩阵的探索

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编辑学报 2024年第5期36卷 544-547页

作者：焦爽中医杂志社北京100700

以《中医杂志》融合出版工作中的实践与探索为例,阐释科技期刊应根据自身定位确立融合出版方向,充分利用现有资源开展融合出版工作,针对不同需求灵活地运用多种媒体形式传播学术内容,借助现代技术实现刊网一体化传播矩阵运营,提高个性... 详细信息

以《中医杂志》融合出版工作中的实践与探索为例,阐释科技期刊应根据自身定位确立融合出版方向,充分利用现有资源开展融合出版工作,针对不同需求灵活地运用多种媒体形式传播学术内容,借助现代技术实现刊网一体化传播矩阵运营,提高个性化用户服务水平。同时,提出期刊应重视融合出版内容制作的创新与深耕、重视学术社交服务、重视版权保护,以期为科技期刊打造媒体融合出版矩阵提供参考。

关键词：融合出版科技期刊学术影响力学术传播刊网一体化

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物流产业集聚对碳排放跨区域转移的作用机制

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中国环境科学 2021年第7期41卷 3441-3452页

作者：王健林双娇福州大学经济与管理学院福建福州350108

基于2002~2015年中国多区域投入产出表(MRIO)数据,结合中介效应和动态门槛效应模型,检验物流产业集聚对物流业碳转移的影响机制,并拓展性地就物流产业集聚对物流业碳转移方向的非对称性影响进行研究.结果表明:物流产业集聚与碳转移之间... 详细信息

基于2002~2015年中国多区域投入产出表(MRIO)数据,结合中介效应和动态门槛效应模型,检验物流产业集聚对物流业碳转移的影响机制,并拓展性地就物流产业集聚对物流业碳转移方向的非对称性影响进行研究.结果表明:物流产业集聚与碳转移之间呈显著的正向关系.物流产业集聚不仅直接促进碳转移,还通过信息化运作和交通运输压力作用于物流业碳转移.信息化运作约束下,物流产业集聚对物流业碳转移的影响总体呈倒U字型特征;交通运输压力作用下,物流产业集聚对物流业碳转移的影响呈梯度式增强特征.物流产业集聚对物流业碳转移的影响在方向上具有非对称性特征.

关键词：物流产业集聚碳转移作用机制中介效应模型门槛效应模型

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