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Dose-dependent effects of lead on cell-cycle arrest, DNA damage, and cyclin D1 expression in primary cultured rat hippocampal neurons

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Neural Regeneration Research 2010年第3期5卷 221-225页

作者： shuang gao liguang sun yuanyuan you China Medical University Shenyang 110001 Liaoning Province China

BACKGROUND： Previous studies have suggested that the hippocampus is one of the neurotoxic target sites for lead. However, the molecular mechanisms of action, including the effect of lead on cell-cycle arrest, remain poorly understood. OBJECTIVE： To investigate the effects of different lead concentrations on cell-cycle arrest, DNA damage, and cyclin D1 expression in primary cultured rat hippocampal neurons. DESIGN, TIME AND SETTING： A randomized, controlled, in vitro experiment was performed at the China Medical University between July 2008 and May 2009. MATERIALS： Antibodies specific to cyclin D1 and actin were synthesized and purified by Santa Cruz Biotechnology, USA. FACStar flow cytometer was purchased from Becton Dickinson, San Jose, California, USA. METHODS： Wistar rat hippocampal neurons were primary cultured for 7 days. Neurons in the control group were treated with 0.01 mol/L phosphate buffered saline. Neurons in the 0.2, 1.0, and 10 umol/L lead acetate groups were subjected to 0.2, 1.0, and 10 umol/L lead acetate. Subsequently hippocampal neurons in each group were cultured for 24 hours. MAIN OUTCOME MEASURES： The effects of lead on cell cycle were measured by flow cytometry, DNA damage was measured using the comet assay, and cyclin D1 expression was measured using Western blot analysis. RESULTS： Treatment of hippocampal neurons with 0.2 umol/L lead acetate did not significantly alter cell cycle phase distribution, i.e., sub-G1, S, G0/G1, G2/M, whereas treatment with 1.0 and 10 umol/L lead acetate significantly increased the percentage of S and sub-G1 phase cells （P 〈 0.05）. Olive tail moment in all lead-treated groups and the percentage of DNA in the tail in 1.0 umol/L and 10 umol/L lead acetate groups were significantly greater compared with the control group （P 〈 0.05）. In addition, the percentage of tail DNA was greater in the 0.2 umol/L lead acetate group compared with the control group （P 〉 0.05）. Following incubation with 0.2, 1.0, and 10 umol/L lead

关键词： lead cell-cycle arrest DNA damage cyclin D1 hippocampal neurons nerve factor neural regeneration

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Increased hippocampal Disrupted-In-Schizophrenia 1 expression in mice exposed prenatally to lead

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Neural Regeneration Research 2012年第25期7卷 1939-1945页

作者： yuanyuan you liguang sun Bo Peng Yan Li Songbin Ben shuang gao Department of Biochemical and Molecular Biology China Medical University Shenyang 110001 Liaoning Province China Outpatient Department of China Medical University Shenyang 110001 Liaoning Province China Department of Biochemical and Molecular Biology Jilin Medical College Jilin 132013 Jilin Province China School of Life Science Liaoning University Shenyang 110036 Liaoning Province China School of Public Health China Medical University Shenyang 110001 Liaoning Province China

Disrupted-in-Schizophrenia 1 is a susceptibility gene for schizophrenia and other psychiatric disorders. Developmental lead exposure can cause neurological disorders similar to hyperactivity disorder, dyslexia and schizophrenia. In the present study, we examined the impact of developmental lead exposure, administered in vitro and in vivo, on hippocampal Disrupted-In- Schizophrenia 1 expression. Our results show that in cultured hippocampal neurons, in vitro exposure to 0.1-10 pM lead, inhibited neurite growth and increased Disrupted-In-Schizophrenia 1 mRNA and protein expression dose-dependently. In addition, blood lead levels in mice were increased with increasing mouse maternal lead （0.01-1 mM） exposure. Hippocampal neurons from these mice showed a concomitant increase in Disrupted-in-Schizophrenia 1 mRNA and protein expression. Overall our findings suggest that in vivo and in vitro lead exposure increases Disrupted-In-Schizophrenia 1 expression in hippocampal neurons dose-dependently, and consequently may influence synapse formation in newborn neurons.

关键词： lead exposure Disrupted-In-Schizophrenia 1 hippocampus neuron neurotoxicity synapse neuralregeneration

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Recent Progress in Design of Organic Electro-optic Materials with Ultrahigh Electro-optic Activities

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Chinese Journal of Chemistry 2022年第24期40卷 3001-3012页

作者： Huajun Xu Yanna sun yuanyuan Kan Ke gao Science Center for Material Creation and Energy Conversion Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdaoShandong266237 China

Rationally designed organic electro-optic (OEO) materials demonstrate ultra-large electro-optic (EO) activities, affording inorganic-organic hybrid photonic devices with low drive voltage, large bandwidth, low energy consumption, and small footprint. OEO materials hold the potential to achieve EO coefficients (r_(33)) over 1000 pm/V. Over the past decade, however, the best performance of OEO materials is limited to 300—600 pm·V^(−1). This is partly because of the concern of increasing dipole moment and optical loss due to the redshifted absorbance of high hyperpolarizability chromophores. Recent advance of theory-guided design enables the OEO materials to achieve greatly enhanced hyperpolarizability and EO activity with dipole moment and propagation loss within acceptable constraints. Simultaneously, progress in hybrid device designs has greatly shortened the length of modulating waveguide, which resulted in significantly reduced sensitivity to propagation loss from redshifted absorption of OEO materials. Driven by theory-guided design method, several high-performance OEO materials have been presented with greatly enhanced EO coefficients beyond 1000 pm·V^(−1). This brief review summarizes the strategies to improve the EO activity including molecular engineering and hyperpolarizability, highlights the recent great progress in design of high-performance OEO materials, and discusses the problems needed to be solved in application for current OEO materials.

关键词： Nonlinear optics Chromophores Donor-acceptor systems Electro optic Materials Electro-optic Modulator

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Single-junction organic solar cell smashes performance record

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Science China Materials 2022年第9期65卷 2609-2610页

作者： Yanna sun Huajun Xu yuanyuan Kan Ke gao Science Center for Material Creation and Energy Conversion Institute of Frontier and Interdisciplinary ScienceShandong UniversityQingdao 266237China

Organic solar cells(OSCs)have become a major focus in the field of the third-generation renewable energy thanks to their outstanding advantages of low cost,light weight and *** recent years,efforts dedicated to OSCs via material design,morphology optimization,and mechanism analysis have made remarkable progress,with power conversion efficiency(PCE)exceeding 19%.However,compared with inorganic solar cells(e.g.,Si or perovskite),the performance of OSCs remains limited by non-ideal exciton and charge transport.

关键词： exceeding performance record

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Rapid and non-destructive decay detection of Yali pears using hyperspectral imaging coupled with 2D correlation spectroscopy

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International Journal of Agricultural and Biological Engineering 2022年第5期15卷 236-244页

作者： Yufan Zhang Wenxiu Wang Fan Zhang Qianyun Ma shuang gao Jie Wang Jianfeng sun yuanyuan Liu College of Food Science and Technology Hebei Agricultural UniversityBaoding 071000HebeiChina Agricultural Engineering Key Laboratory at Universities of Education Department of

The black spot disease caused by Alternaria alternata on Yali pears is a great concern as it compromises their edible quality and commercial *** realize rapid and non-destructive classification of this disease,hyperspectral imaging(HSI)technology was combined with two-dimensional correlation spectroscopy(2DCOS)analysis.A total of 150 pear samples at different decay grades were *** obtaining the HSI images,the whole sample was demarcated as the region of interest,and the spectral information was *** preprocessing methods were applied and compared to build the classification ***,using the inoculation day as an external perturbation,2DCOS was used to select the feature-related wavebands for black spot disease identification,and the result was compared to those obtained using competitive adaptive reweighting sampling and the successive projections *** demonstrated that the simplified least squares support vector model based on 2DCOS-identified feature wavebands yielded the best performance with the identification accuracy,precision,sensitivity,and specificity of 97.30%,94.60%,96.16%,and 98.21%,***,2DCOS can effectively interpret the feature-related wavebands,and its combination with HSI is an effective tool to predict black spot disease on Yali pears.

关键词： hyperspectral imaging technology black spot disease two-dimensional correlation spectroscopy Yali pear

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Secondary metabolites of mulberry leaves exert anti-lung cancer activity through regulating the PD-L1/PD-1 signaling pathway

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Journal of Pharmaceutical Analysis 2024年第6期14卷 914-925页

作者： Guiqin Ye Xin sun Jiuzhou Li yuanyuan Mai Ruilan gao Jianbin Zhang Cancer Center Department of Medical OncologyKey Laboratory of Tumor Molecular Diagnosis and Individualized Medicine of Zhejiang ProvinceZhejiang Provincial People's HospitalAffiliated People's HospitalHangzhou Medical CollegeHangzhou310014China Clinical Laboratory Yuhuan City HospitalTaizhouZhejiang317600China Hangzhou Medical College Hangzhou311300China Department of Neurosurgery Binzhou People's HospitalBinzhouShandong256600China Institute of Hematology Research The First Affiliated Hospital of Zhejiang Chinese Medical UniversityHangzhou310060China

Lung cancer ranks the top of malignancies that cause cancer-related deaths *** leaves of Morus alba L are traditional Chinese medicine widely applied in respiratory *** previous work has demonstrated the anti-lung cancer effect of secondary metabolites of mulberry leaf,but their mechanism of action has still not fully *** synthesized Moracin N(MAN)-Probe conjugated with alkyne to label lung cancer cells and identified protein targets by chemical proteomic *** and its probe exerted similar growth-inhibitory effect on human lung cancer *** proteomic results showed that MAN targeted the programmed death ligand 1(PD-L1)checkpoint pathway and T cell receptor(TCR)signaling pathway,indicating its immune-regulatory ***-free surface plasmon resonance(SPR)results showed the direct interaction of MAN with PD-L1 *** docking analysis demonstrated that MAN bound to E158 residue of PD-L1 *** downregulated the expression levels of PD-L1 in a time-and dose-dependent manner and disrupted the PD-L1/programmed death 1(PD-1)binding,including other secondary metabolites of mulberry leaves Guangsangon E(GSE)and Chalcomoracin(CMR).Human peripheral blood mononuclear cells(PBMCs)co-cultured with MAN-treated A549 cells,resulting in the increase of CD8^(+)GZMB^(+)T cells and the decrease of CD8^(+)PD-1^(+)T *** suggested that MAN exerts anti-cancer effect through blocking the PD-L1/PD-1 *** vivo,MAN combined with anti-PD-1 antibody significantly inhibited lung cancer development and metastasis,indicating their synergistic *** together,secondary metabolites of mulberry leaves target the PD-L1/PD-1 signaling,enhance T cell-mediated immunity and inhibit the tumorigenesis of lung *** modulatory effect on tumor microenvironment makes them able to enhance the therapeutic efficacy of immune checkpoint inhibitors in lung cancer.

关键词： Moracin N Click chemistry PD-L1 T cell Lung cancer

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Fabrication of(TiB/Ti)-TiAl composites with a controlled laminated architecture and enhanced mechanical properties

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Journal of Materials Science & Technology 2021年第3期62卷 221-233页

作者： Hao Ding Xiping Cui Naonao gao Yuan sun yuanyuan Zhang Lujun Huang Lin Geng School of Materials Science and Engineering Harbin Institute of TechnologyP.O.Box 433Harbin150001China Division of Superalloys Institute of Metal ResearchChinese Academy of SciencesShenyang110016China

The(TiB/Ti)-TiAl composites with a laminated structure composing of alternating TiB/Ti composite layers,α_(2)-Ti_(3)Al interfacial reaction layers of andγ-TiAl layers were successfully pre pared by spark plasma sintering of alternately stacked Tib_(2)/Ti powder layers and TiAl powder *** the influence of thickness ratio of Tib_(2)/Ti powder layers to TiAl powder layers on microstructure evolution and mechanical properties of the re sulting(TiB/Ti)-TiAl laminated composites were investigated *** results showed that the thickening ofα_(2)-Ti_(3)Al layers which originated from the reaction of Ti and TiAl was significantly hindered by introducing Tib_(2)particles into starting Ti *** the thickness ratio of Tib_(2)/Ti powder layers to TiAl powder layers increased,the bending fracture strength and fracture toughness at room temperature of the final(TiB/Ti)-TiAl laminated composites were remarkably improved,especially for the(TiB/Ti)-TiAl composites prepared by Tib_(2)/Ti powder layers with thickness of 800μm and TiAl powder layers with thickness of 400μm,whose fracture toughness and bending strength were up to 51.2 MPa·m^(1/2)and 1456 MPa,respectively,293%and 108%higher than that of the monolithic TiAl alloys in the present *** was attributed to the addition of high-performance network TiB/Ti composite ***,it was noteworthy that the ultimate tensile strength at 700℃of(TiB/Ti)-TiAl composites fabricated by 400μm thick Tib_(2)/Ti powder layers and 400μm thick TiAl powder layers was as high as that at 550℃of network TiB/Ti *** means the service temperature of(TiB/Ti)-TiAl laminated composites was likely raised by 150℃,meanwhile a good combination of high strength and high toughness at ambient tempe rature could be ***,the fracture mechanism of(TiB/Ti)-TiAl laminated composites was proposed.

关键词： Titanium aluminide matrix composites Laminated structure High temperature properties Strengthening-toughening

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Identifying the effective combination of acupuncture and traditional Chinese medicinal herbs for postmenopausal osteoporosis therapy through studies of their molecular regulation of bone homeostasis

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Journal of Traditional Chinese Medicine 2024年第1期44卷 212-219页

作者： REN Ping WANG Quanwu BAI Wei sun Miao LIU Zheling gao Ming WANG Liang PENG Bo XU liguang Department of Health Management the Third Affiliated Clinical Hospital of Changchun University of Chinese MedicineChangchun 130117China Department of Dirty Tuina the affiliated Hospital to Changchun University of Chinese MedicineChangchun 130117China Department of Acupuncture the affiliated Hospital to Changchun University of Chinese MedicineChangchun 130117China Department of Rehabilitation Medicine the 924th Hospital of the PLA Joint Logistic Support ForceFoshan 528226China Department of College of Acupuncture and Massage Changchun University of Chinese MedicineChangchun 130117China

Worldwide,as the population age,osteoporosis is becoming increasingly common,and osteoporotic fractures have a significant economic *** women are the most susceptible to developing osteoporosis and the most critical time to prevent it is during the perimenopausal and early menopausal *** this regard,we hypothesize rational combination of acupuncture and Traditional Chinese Medicine(TCM)in the form of herbal extract could prevent osteoporosis in *** deficiency during menopause causes low-level inflammation that stimulates the formation of osteoclasts,the boneresorbing cells,and simultaneously inhibits the viability and function of osteoblasts,the bone-forming *** most potent inflammatory cytokine in skeletal homeostasis is the receptor activator of nuclear factor kappa B ligand(RANKL)that stimulates osteoclast ***,the canonical Wnt pathway is essential for osteoblastogenesis and bone formation,and estrogen deficiency leads to diminished functioning of this *** and acupuncture could target the RANKL and the Wnt pathway in favorable ways to prevent the accelerated bone loss experienced during the early menopausal stage and promote the gain in bone mass in postmenopausal *** this review,we propose a rational combination of specific TCM and acupuncture targeting those signaling molecules/pathways by the drugs that are in clinical use for the treatment of postmenopausal *** rational approach revealed that Danshen(Radix Salviae Miltiorrhizae)could exert a synergistic effect with *** then propose a translational path for developing the putative combination in women with postmenopausal osteoporosis to curtail the risk of osteoporotic fractures.

关键词： medicine traditional osteogenic anti-resorptive review

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Enhancing efficiency and stability of organic solar cells through a simplified four-step synthesis of fully non-fused ring electron acceptor

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Journal of Energy Chemistry 2024年第6期93卷 601-608,I0015页

作者： Chenyang Han Huanhuan gao Yanna sun yuanyuan Kan Zhaozhao Bi Wei Ma Yani Zhang Juan Antonio Zapien Yingguo Yang Ke gao College of New Energy Xi’an Shiyou UniversityXi’an 710065ShaanxiChina Shandong Provincial Key Laboratory for Science of Material Creation and Energy Conversion Institute of Frontier ChemistrySchool of Chemistry and Chemical EngineeringShandong UniversityQingdao 266237ShandongChina State Key Laboratory for Mechanical Behavior of Materials Xi’an Jiaotong UniversityXi’an 710049ShaanxiChina Department of Materials Science and Engineering City University of Hong KongKowloonHong Kong 99907China School of Microelectronics Fudan UniversityShanghai 200433China

Design and synthesis of superior cost-effective non-fullerene acceptors(NFAs)are still big challenges for facilitating the commercialization of organic solar cells(OSCs),yet to be ***,two medium bandgap fully non-fused ring electron acceptors(NFREAs,medium bandgap,i,e.,1,3-1,8 eV),namely PTR-2Cl and PTR-4Cl are synthesized with only four steps by using intramolecular noncovalent interaction central core,structured alkyl side chain orientation linking units and flanking with different electron-withdrawing end *** them,PTR-4C1 exhibits increased average electrostatic potential(ESP)difference with polymer donor,enhanced crystallinity and compactπ-πstacking compared with the control molecule *** a result,the PTR-4Cl-based OSC achieved an impressive power conversion efficiency(PCE)of 14.72%,with a much higher open-circuit voltage(V_(OC))of 0.953 V and significantly improved fill factor(FF)of 0.758,demonstrating one of the best acceptor material in the top-performing fully NFREA-based OSCs with both high PCE and V_(OC).Notably,PTR-4Cl-based cells maintain a good T_80lifetime of its initial PCE after over 936 h under a continuous thermal annealing treatment and over1300 h T_(80)lifetime without *** work provides a cost-effective design strategy for NFREAs on obtaining high V_(OC),efficient exciton dissociation,and ordered molecular packing and thus high-efficiency and stable OSCs.

关键词： Organic solar cells Fully non-fused ring acceptors End group engineering Morphology regulation High efficiency

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Silane or Siloxane-Side-Chain Engineering of Photovoltaic Materials for Organic Solar Cells

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Chinese Journal of Chemistry 2023年第24期41卷 3703-3713页

作者： Qian Wang Xu Zhang Wentao Zou Huajun Xu yuanyuan Kan Yanna sun Ke gao Shandong Provincial Key Laboratory for Science of Material Creation and Energy Conversion Science Center for Material Creation and Energy ConversionInstitute of Frontier and Interdisciplinary ScienceShandong UniversityQingdaoShandong266237 China

With the tactful material design,skillful device engineering,and in-depth understanding of morphology optimization,organic solar cells (OSCs) have achieved considerable ***,OSCs have reached high power conversion efficiencies (PCEs) exceeding 19%.Especially,continuously emerging new materials have been considered as one of the key factors to improve the PCEs of *** molecular design strategies,side-chain engineering is an easy and commonly-used means which can optimize the solubility,alter intermolecular stacking arrangement,fine-tune the open circuit voltage (VOC),thus ultimately improve the *** hybrid side chains,silane and siloxane side chains have considerable effects,not only in increasing the carrier mobility and tuning the energy level,but also in affecting the crystallinity and molecular *** this review,the latest developments in photovoltaic materials based on silane and siloxane side chains are presented to illustrate the structure-property *** review comprehensively includes silane-side based polymer/small molecule donors;siloxane-side based polymer/small molecule donors,and polymer/small molecule *** the similarities and differences between these two side chains are ***,the possible applications and future prospects of silane and siloxane side chains are presented.

关键词： Organic solar cell Silane side chain Siloxane side chain Side chain engineering High performance Batteries Applications

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