检索结果-南通市图书馆

Unsynchronized butyrophilin molecules dictate cancer cell evasion of Vγ9Vδ2 T-cell killing

Cellular & Molecular Immunology 2024年第4期21卷 362-373页

作者： Zeguang Wu Qiezhong Lamao Meichao Gu Xuanxuan Jin Ying Liu Feng Tian Ying Yu Pengfei Yuan shuaixin gao Thomas S.Fulford Adam P.Uldrich Catherine C.L Wong Wensheng Wei Biomedical Pioneering Innovation Center Peking-Tsinghua Center for Life SciencesPeking University Genome Editing Research CenterState Key Laboratory of Protein and Plant Gene ResearchSchool of Life SciencesPeking University100871BeijingChina EdiGene Inc. Life Science ParkChangping District102206BeijingChina Center for Precision Medicine Multi-Omics Research Peking University Health Science CenterPeking University100191BeijingChina Department of Microbiology and Immunology at the Peter Doherty Institute for Infection and Immunity The University of MelbourneParkvilleVIC3010Australia Cancer Immunology Program Peter MacCallum Cancer CentreMelbourneVICAustralia State Key Laboratory for Complex Severe and Rare DiseasesClinical Research InstitutePeking Union Medical College HospitalChinese Academy of Medical Science&Peking Union Medical CollegePeking-Tsinghua Center for Life Sciences100871BeijingChina

Vγ9Vδ2 T cells are specialized effector cells that have gained prominence as immunotherapy agents due to their ability to target and kill cells with altered pyrophosphate *** our effort to understand how cancer cells evade the cell-killing activity of Vγ9Vδ2 T cells,we performed a comprehensive genome-scale CRISPR screening of cancer *** found that four molecules belonging to the butyrophilin(BTN)family,specifically BTN2A1,BTN3A1,BTN3A2,and BTN3A3,are critically important and play unique,nonoverlapping roles in facilitating the destruction of cancer cells by primary Vγ9Vδ2 T *** coordinated function of these BTN molecules was driven by synchronized gene expression,which was regulated by IFN-γsignaling and the RFX ***,an enzyme called QPCTL was shown to play a key role in modifying the N-terminal glutamine of these BTN proteins and was found to be a crucial factor in Vγ9Vδ2 T cell killing of cancer *** our research,we offer a detailed overview of the functional genomic mechanisms that underlie how cancer cells escape Vγ9Vδ2 T ***,our findings shed light on the importance of the harmonized expression and function of gene family members in modulating T-cell activity.

关键词： Butyrophilin Vγ9Vδ2 T-cell Cancer-specific immune evasion Glutaminyl-peptide cyclotransferase-like Pyrophosphate metabolite Immunotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multiple Signaling Roles of CD3ε and Its Application in CAR-T Cell Therapy

Multiple Signaling Roles of CD3ε and Its Application in CAR...

引用

第十四届全国免疫学学术大会

作者： Wei Wu Qiuping Zhou Takeya Masubuchi Xiaoshan Shi Hua Li Xinyi Xu Min Huang Li Meng Xing He Hengyu Zhu shuaixin gao Nan Zhang Ruirui Jing Jie Sun Haopeng Wang Enfu Hui Catherine Chiulan Wong Chenqi Xu the First Hospital of Jilin University State Key Laboratory of Molecular Biology Shanghai Institute of Biochemistry and Cell Biology Center for Excellence in Molecular Cell Science Chinese Academy of Sciences Section of Cell & Developmental Biology Division of Biological Sciences University of California Center for Precision Medicine Multi-omics Research Peking University Health Science Center Peking University First Hospital Bone Marrow Transplantation Center of the First Affiliated Hospital and Department of Cell Biology Zhejiang University School of Medicine School of Life Science and Technology ShanghaiTech University

A T cell receptor(TCR) mediates antigen-induced signaling through its associated CD3ε, δ, γ, and ζ, but the contributions of different CD3 chains remain elusive. Using quantitative mass spectrometry, we simultaneously quantitated the phosphorylation of the immunoreceptor tyrosine-based activation motif(ITAM) of all CD3 chains upon TCR stimulation. A subpopulation of CD3ε ITAMs was mono-phosphorylated, owing to Lck kinase selectivity, and specifically recruited the inhibitory Csk kinase to attenuate TCR signaling, suggesting that TCR is a self-restrained signaling machinery containing both activating and inhibitory motifs. Moreover, we found that incorporation of the CD3ε cytoplasmic domain into a second-generation chimeric antigen receptor(CAR) improved antitumor activity of CAR-T cells. Mechanistically, the Csk-recruiting ITAM of CD3ε reduced CAR-T cytokine production whereas the basic residue rich sequence(BRS) of CD3ε promoted CAR-T persistence via p85 recruitment. Collectively, CD3ε is a built-in multifunctional signal tuner, and increasing CD3 diversity represents a strategy to design next-generation CAR.

关键词： CAR-T therapy Csk ITAM T cell receptor TCR cancer cell persistence cytokine release p85 phosphorylation pattern quantitative mass spectrometry

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

基于UPLC-PDA指纹图谱评价白蒲黄不同复方制剂的质量

引用

常州大学学报（自然科学版） 2019年第6期31卷 82-87,93页

作者：高帅鑫周晓鹰常州大学制药与生命科学学院

建立白蒲黄复方制剂(配方颗粒、片剂及胶囊)的超高效液相色谱-二极管阵列检测(UPLC-PDA)指纹图谱,为全面控制白蒲黄复方制剂提供参考。将白蒲黄不同复方制剂加70%甲醇超声60 min;提取物的分析采用Agilent Poroshell 120 EC-C18柱(直径2.... 详细信息

建立白蒲黄复方制剂(配方颗粒、片剂及胶囊)的超高效液相色谱-二极管阵列检测(UPLC-PDA)指纹图谱,为全面控制白蒲黄复方制剂提供参考。将白蒲黄不同复方制剂加70%甲醇超声60 min;提取物的分析采用Agilent Poroshell 120 EC-C18柱(直径2.1 mm×长度150 mm,C18粒径2.7μm),流速1.0 mL·min-1,柱温40℃,检测波长254 nm,流动相为甲醇(B)-0.1%甲酸水溶液(A),采用梯度洗脱0~15 min:5%~40%B;15~35 min:40%B;35~50 min:40%~70%B;50~55 min:70%B;55~62 min:70%~100%B;62~75 min:100%B。分别对不同厂家、剂型、批次的白蒲黄复方制剂进行相似度拟合,标定了白蒲黄不同复方制剂指纹图谱的24个共有峰,并对白蒲黄制剂的色谱条件及提取方法进行优化,其精密度、稳定性、重复性的变异系数(RSD)值均小于2%;测得的10批次样品与拟合指纹图谱相似度均大于0.95。所建立的指纹图谱可为规范白蒲黄不同复方制剂的均一性和稳定性的质量控制提供参考依据。

关键词：白蒲黄复方制剂指纹图谱 UPLC-PDA 质量控制

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

采动覆岩离层注浆对矿井安全的影响评价

引用

煤炭技术 2022年第8期41卷 170-174页

作者：姚文涛寇天昊樊斌高泽鑫岳鹏温帅鑫中煤地质集团有限公司北京100043 中国地质大学(北京)工程技术学院北京100083 山西潞安环保能源开发股份有限公司五阳煤矿山西长治046200

煤矿采动覆岩离层注浆技术中,注浆层位与采场覆岩导水裂隙带的水力联系是影响矿井安全的重要因素。为了评价五阳煤矿采动覆岩离层注浆对矿井安全的影响,通过覆岩岩性特征及富水空间性质划分3^(#)煤层上覆岩体含、隔水层层位,利用地震及... 详细信息

煤矿采动覆岩离层注浆技术中,注浆层位与采场覆岩导水裂隙带的水力联系是影响矿井安全的重要因素。为了评价五阳煤矿采动覆岩离层注浆对矿井安全的影响,通过覆岩岩性特征及富水空间性质划分3^(#)煤层上覆岩体含、隔水层层位,利用地震及测井等物探方法探明工作面范围内断裂构造的分布,采用经验公式法和数值模拟法综合确定采场覆岩导水裂隙带的发育高度。基于以上研究,综合分析论证了注浆层位与导水裂隙带间的隔水层保护能力及断层能否使注浆层位与导水裂隙带或煤层间产生水力联系,结果表明,导水裂隙带顶部与注浆位置之间有2层较厚的隔水层阻断其水力联系,构造离层注浆浆液及浆液析出水不会通过工作面范围内的7处断层进入导水裂隙带或矿井内,本工作面实施的采动覆岩离层注浆施工不会对矿井安全造成影响。

关键词：离层注浆矿井安全导水裂隙带浆液渗漏

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：