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转录因子Afndt6对黄曲霉毒力和侵染力的影响

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河南工业大学学报（自然科学版） 2024年第3期45卷 9-16页

作者：兰海儿梁柳柯魏闪雷阳张帅兵胡元森吕扬勇河南工业大学生物工程学院河南郑州450001

为探究转录因子Afndt6对黄曲霉生长发育、毒素生物合成及侵染粮食籽粒能力的影响,通过同源重组构建Afndt6基因缺失的黄曲霉菌株,采用体视显微镜和切片观察手段研究Afndt6基因缺失对黄曲霉生长发育和孢子产生的影响,采用薄层色谱层析测... 详细信息

为探究转录因子Afndt6对黄曲霉生长发育、毒素生物合成及侵染粮食籽粒能力的影响,通过同源重组构建Afndt6基因缺失的黄曲霉菌株,采用体视显微镜和切片观察手段研究Afndt6基因缺失对黄曲霉生长发育和孢子产生的影响,采用薄层色谱层析测定黄曲霉毒素生物合成量,以花生和玉米籽粒为基质评价基因缺失菌株的侵染力。结果表明:与对照菌株相比,ΔAfndt6菌株的生长速度、分生孢子的产量没有显著变化,但菌落分生孢子颜色变为白色;基因缺失菌株对细胞壁胁迫试剂刚果红和氧化胁迫压力不敏感,200μg/mL刚果红处理后对照菌株和ΔAfndt6菌株的生长抑制率分别为31.10%和25.81%,15 mmol/L H_(2)O_(2)处理后对照菌株和ΔAfndt6菌株的生长抑制率分别为47.94%和17.05%;ΔAfndt6菌株的黄曲霉毒素产量显著升高,对花生、玉米种子的侵染能力增强。Afndt6基因编码的转录因子能够影响黄曲霉生长发育,负调控黄曲霉毒素的生物合成,可为储粮过程中防控黄曲霉及黄曲霉毒素污染提供参考。

关键词： Afndt6 黄曲霉黄曲霉毒素侵染力

Insights into the mechanism of Sub3 inhibiting Fusarium moniliforme infection in maize

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Grain & Oil Science and Technology 2022年第1期5卷 22-34页

作者： Haojie Yang Wei zhang Pingping Tian Bangbang Li Shan Wei shuaibing zhang Na Li Yangyong Lyu Yuansen Hu College of Biological Engineering Henan University of TechnologyZhengzhou 450001China Henan Provincial Key Laboratory of Biological Processing and Nutritional Function of Wheat Zhengzhou 450001China

Fusarium moniliforme(***) and its secondary metabolite fumonisin pose a severe threat to food safety,and searching for effective antimicrobial agents is a focus of current *** this study,the secondary structure of Sub3 was analyzed by circular dichroism,meanwhile,the inhibition rate of Sub3 against spores,mycelia of *** and infected maize was *** explore the possible inhibition mechanisms,morphological and structural changes of spores treated with Sub3 at0,1/2 MIC(minimum inhibitory concentration) and MIC were observed by scanning electron microscopy and transmission electron microscopy;the cell wall integrity,membrane integrity,reactive oxygen species,mitochondrial membrane potential,ATP synthase activity,redox reactions,and the nuclear damage of *** were also *** results showed that Sub3 was mostly in the state of random in deionized water,while mainly showed the β-sheet structure in the hydrophobic environment of 50% Trifluoroethanol(TFE) solution,indicating that Sub3 might generate partial structure deformation when acting on the cell membrane;and its MIC on *** spores was 0.2 g/*** the 1/2 MIC and MIC,the inhibition rates of Sub3 against *** infected maize were 34.3% and75.6%,*** results of inhibition mechanisms revealed that the defective pathogenicity of *** caused by Sub3 was attributed to damages on both the cell wall and the cell membrane,which might upset balance of intracellular redox system and mitochondrial energy metabolism and trigger nucleus damage,ultimately leading to cell ***,Sub3 could diminished ATP synthase enzyme activity in a dose-dependent *** results provided direct evidence for inhibition of *** infection of maize by Sub3,and useful knowledge applicable for food preservation.

关键词： Sub3 Antifungal activity Inhibition mechanism F.moniliforme Maize

Characteristics of Paleontological Communities in Surface Sediments of the Southern South China Sea and Their Paleoclimatic and Paleoenvironmental Significance

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Journal of Earth Science 2024年第1期35卷 144-154页

作者： Jing Zhao Li zhang Xiaoyan Wang Jinglian Ge Min Xu Wei Chen Mao Luo Qianyong Liang Qiuhua Yu shuaibing Luo Wenkun Qie Key Laboratory of Marine Mineral Resoures Ministry of Natural ResourcesGuangzhou Marine Geological SurveyChina Geological SurveyGuangzhou 511458China National Engineering Research Center for Gas Hydrate Exploration and Development Guangzhou 511458China State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008China

Understanding the spatial and temporal distribution of different paleontological communities in the southern South China Sea(SCS)is fundamental to explore its paleoclimatic and paleoenvironmental *** this study,foraminifera,pollen and diatoms from 100 surface sediment samples covering 40000 km^(2) of sea floor in the southern SCS were comprehensively investigated in terms of their assemblage and *** results showed the existence of abundant foraminifera and pollen in most of the samples,although diatom communities were relatively *** were dominated by ***,***,***,while diatoms were characterized by ***,*** and ***,indicating a typical tropical marine *** pollen assemblages showed a better reflection of montane rainforest,tropical rainforest and *** spatial differences among foraminifera assemblages may indicate the effect of water depth and a warmer environment in the southeast part of the study area,while the spatial patterns of diatom and pollen assemblages imply the influence of coastal *** study also noted that the compositions of paleontological communities in the SCS can vary significantly in a short distance,and synthesized studies on multiple biological groups are needed to reconstruct the Quaternary climate and the oceanographic environment.

关键词： southern South China Sea sediments foraminifera diatom pollen

Effects of hexanal fumigation on fungal spoilage and grain quality of stored wheat

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Grain & Oil Science and Technology 2021年第1期4卷 10-17页

作者： shuaibing zhang Minghui Zheng Huanchen Zhai Ping'an Ma Yangyong Lyu Yuansen Hu Jingping Cai College of Biological Engineering Henan University of TechnologyZhengzhou 450001China

The prevention of fungal spoilage is an essential consideration in wheat *** studies have revealed that volatile organic compounds(VOCs),possibly with natural fungicidal properties,could be produced from stored wheat *** this study,the antifungal effect of hexanal,a main component of VOCs from stored wheat,against spoilage fungi on agar plate and in high-moisture wheat grains were investigated via the gas fumigation *** the impact of hexanal fumigation on grain quality was evaluated through analysis of the malondialdehyde content,fatty acid values,germination percentages and vigor of 16%and 18%moisture wheat grains fumigated with 1.66,2.49,and 3.31 mmol/L hexanal *** results of in vitro antifungal experiments on agar plates revealed that the minimum inhibitory concentration and minimum fungicidal(fatal)concentration of hexanal against the five fungi were 4–14 folds and 4–7 folds lower than those of propionic acid,*** fungal spoilage of high-moisture wheat grains inoculated with pure fungal spores and with naturally occurring fungi could be completely inhibited by 1.66 mmol/L hexanal *** 5-week storage of high-moisture wheat grains fumigated with 1.66,2.49,and 3.31 mmol/L hexanal vapor,the malondialdehyde content in high-moisture wheat grains did not change significantly in all samples,and fatty acid values were slightly higher in 18%moisture wheat than in 16%moisture *** germination percentages and vigor of wheat samples decreased with increased hexanal vapor concentrations and moisture *** results indicated that hexanal fumigation could be used as an alternative chemical control method to prevent the fungal spoilage of postharvest wheat.

关键词： Hexanal Chemical control Spoilage fungi Wheat storage Grain quality

Correlation of Gluten Molecular Conformation with Dough Viscoelastic Properties during Storage

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Grain & Oil Science and Technology 2018年第1期1卷 1-7页

作者： JIA Feng zhang shuaibing zhang Yijun WANG Jinshui College of Biological Engineering Henan University of Technology Innovative Experimental College Northwest A&F University

Significant correlations exist for the total sample between the dough viscoelastic properties and quality, but little information about these links is available. This study investigates the relationship between the gluten molecular conformation and the dough viscoelastic properties of the Zhengmai 379 wheat variety with different relative humidity(RH) values during *** results showed that protein extractability, free sulfhydryl(SH) groups, and wet gluten contents decreased to a certain degree during storage, while the glutenin quantity significantly varied. Significant negative correlation coefficients were found between the dough viscosity at 50% RH and the β-turn contents at 40% RH(-0.918) or 60% RH(-0.949)(P < 0.01), the dough viscosity at50% RH and the β-turn contents at 50% RH(-0.912)(P < 0.05), and the dough viscosity at 50% RH and the α-helix/β-sheet ratio at 40% RH(-0.875), 50% RH(-0.869), or 60% RH(-0.843)(P < 0.05). Significant correlation coefficients were further observed between the dough viscosity at 50% RH and the β-sheet contents at 60% RH(0.927)(P < 0.01) and between the dough viscosity at50% RH and the β-sheet contents at 40% RH(0.910) or 50% RH(0.908)(P < 0.05). A decrease in the free SH groups of gluten and an increase in the low-molecular weight contents suggested that gliadin was incorporated via SS crosslinking to the glutenin-like protein, which did not dissolve in ethanol.

关键词： Wheat gluten Viscoelastic properties Free sulfhydryl groups Gluten secondary structure

AnHog1基因缺失对黑曲霉中赭曲霉毒素A生物合成的影响

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河南工业大学学报（自然科学版） 2023年第6期44卷 76-82页

作者：张艺格魏闪吕扬勇翟焕趁张帅兵胡元森河南工业大学生物工程学院河南郑州450001

黑曲霉菌株能够产生强致癌的赭曲霉毒素A(ochratoxin A,OTA),对食品安全和人类健康存在巨大隐患。为深入研究OTA生物合成调控机理,构建了黑曲霉AnHog1基因缺失菌株,通过高效液相色谱-荧光检测器检测该基因对OTA合成的影响,采用荧光定量... 详细信息

黑曲霉菌株能够产生强致癌的赭曲霉毒素A(ochratoxin A,OTA),对食品安全和人类健康存在巨大隐患。为深入研究OTA生物合成调控机理,构建了黑曲霉AnHog1基因缺失菌株,通过高效液相色谱-荧光检测器检测该基因对OTA合成的影响,采用荧光定量PCR技术探究该基因缺失对OTA生物合成簇内基因表达水平的影响,并检测对过氧化氢(H_(2)O_(2))耐受性与活性氧(ROS)水平的影响。结果表明:AnHog1基因缺失不影响黑曲霉生长及产孢,但显著抑制OTA生物合成,OTA含量下降了40.8%;敲除AnHog1基因后,与OTA合成基因簇相关的基因pks、p450、nrps、hal和bzip的表达水平分别下调了64.19%、25.82%、18.88%、51.31%和48.92%;在H_(2)O_(2)胁迫条件下,AnHog1基因缺失菌株对H2O2敏感性增加,且菌丝体内ROS水平升高。AnHog1基因缺失后可能通过抑制OTA合成基因的表达和破坏氧化还原平衡抑制OTA生物合成,研究结果为挖掘真菌防控新靶标提供科学依据。

关键词：黑曲霉赭曲霉毒素A AnHog1 OTA合成基因簇氧化还原平衡

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双柱体系统局部电场计算的互偶极子模型

电工技术学报 2024年第12期39卷 3869-3883页

作者：张嘉琳康永强蒲绪宏李帅兵兰州交通大学新能源与动力工程学院兰州730070 大型电气传动系统与装备技术国家重点实验室天水741020

两相体局部电场计算模型是研究两相体电场分布、相互作用机制等重要课题的关键,而线电偶极子模型是当前双柱体系统电场计算的经典模型,但当柱体间距较近且介电常数较大时,其计算误差显著增大。针对该问题,该文考虑双柱体系统在均匀电场... 详细信息

两相体局部电场计算模型是研究两相体电场分布、相互作用机制等重要课题的关键,而线电偶极子模型是当前双柱体系统电场计算的经典模型,但当柱体间距较近且介电常数较大时,其计算误差显著增大。针对该问题,该文考虑双柱体系统在均匀电场中的相互作用与极化过程,将处于电场环境中的柱体极化过程分解为外电场极化与相互作用极化两个过程,其中外电场极化结果通过位于柱体轴心的自偶极矩表征,而柱体间极化结果通过一个偏离柱体轴心的互偶极矩表征,并由此构建了双柱体系统互偶极子模型。通过与经典线电偶极子模型及有限元模型计算结果的对比发现,所提的双柱体系统互偶极子模型即使在柱体间距D=0.1R且介电常数之比εi/εe=100时,其计算误差仍小于5%,该结果表明互偶极子模型能够在柱体间距较小且介电常数之比较大时,对双柱体系统的电场分布得到较为精确的计算结果。

关键词：双柱体系统柱体间相互作用电场分布互偶极子模型

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低勘探海域中建南盆地烃源岩早期预测

海洋地质前沿 2024年第4期40卷 39-46页

作者：余秋华张莉刘金萍骆帅兵中国地质调查局广州海洋地质调查局广州511458 自然资源部海底矿产资源重点实验室广州511458 南方海洋科学与工程广东省实验室(广州) 广州511458

烃源岩是油气生成的物质基础。利用最新采集的高品质二维地震资料和综合研究成果,在中建南盆地内划分出4套地震层序,识别了5个不整合地震反射界面。通过分析盆地沉积演化特征,推测中建南盆地发育3套烃源岩,分别为中始新统湖相烃源岩、... 详细信息

烃源岩是油气生成的物质基础。利用最新采集的高品质二维地震资料和综合研究成果,在中建南盆地内划分出4套地震层序,识别了5个不整合地震反射界面。通过分析盆地沉积演化特征,推测中建南盆地发育3套烃源岩,分别为中始新统湖相烃源岩、上始新统—渐新统湖相和海陆交互相烃源岩以及下—中中新统海相烃源岩。通过对其周边盆地进行类比分析,厘定了3套烃源岩的地震相特征;对不同沉积相带泥岩百分含量进行赋值,最终判识推测了中建南盆地新生代3套烃源岩泥岩厚度及分布特征。本研究可为盆地下一步油气资源评价、有利二级构造带分析和勘探部署提供参考。

关键词：中建南盆地低勘探区烃源岩地震相类比法

2016年以来中央政府反“台独”政策的进展、挑战及影响

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统一战线学研究 2024年第4期8卷 98-116页

作者：张建常帅兵上海国际问题研究院上海200233

2016年蔡英文上台以来拒不接受一个中国原则和“九二共识”,导致两岸制度化沟通和协商谈判机制中断,两岸关系面临前所未有的挑战。针对这种变化,中央政府反“台独”工作出现了新的进展:在政治层面相继出台多个反“台独”文件并发布“台... 详细信息

2016年蔡英文上台以来拒不接受一个中国原则和“九二共识”,导致两岸制度化沟通和协商谈判机制中断,两岸关系面临前所未有的挑战。针对这种变化,中央政府反“台独”工作出现了新的进展:在政治层面相继出台多个反“台独”文件并发布“台独”顽固分子清单;军事上形成了对台军事政治高压态势;外交上强化联合国2758号决议的作用,限缩台湾地区的所谓“国际空间”,同时推进中美共管“台独”;经济上推进两岸经济合作,同时采取措施对“台独”分子进行经济制裁;人文层面秉持“两岸一家亲”理念,促进两岸同胞心灵契合。中央反“台独”工作仍然面临民进党长期当政、岛内社会认同变化与外部势力持续干涉的严峻挑战。中央反“台独”政策显示了坚持一个中国原则、维护国家主权的鲜明态度,促进反“台独”、反分裂的态势向有利于内地的方向发展,在国际社会巩固了一个中国格局。未来进一步排除国家统一过程中的政治阻力,中央需要从自身实力、对台政策和国际叙事三个方面继续发力。

关键词：中央政府国家统一反“台独” 一个中国 “九二共识” 民进党当局

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