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ALKBH5 suppresses autophagic flux via N6-methyladenosine demethylation of ZKSCAN3 mRNA in acute pancreatitis

World Journal of Gastroenterology 2024年第12期30卷 1764-1776页

作者： Tao Zhang shuai zhu Geng-Wen Huang Department of General Surgery Xiangya HospitalCentral South UniversityChangsha 410005HunanChina. National Clinical Research Center for Geriatric Disorders Xiangya HospitalCentral South UniversityChangsha 410005HunanChina.

BACKGROUND Increasing evidence has demonstrated that N6-methyladenosine(m6A)RNA modification plays an essential role in a wide range of pathological conditions.Impaired autophagy is a critical hallmark of acute pancreatitis(AP).AIM To explore the role of the m6A modification of ZKSCAN3 in the regulation of autophagy in AP.METHODS The AP mouse cell model was established by cerulein-treated mouse pancreatic acinar cells(MPC-83),and the results were confirmed by the levels of amylase and inflammatory factors.Autophagy activity was evaluated by specific identification of the autophagy-related microstructure and the expression of autophagy-related genes.ZKSCAN3 and ALKBH5 were knocked down to study the function in AP.A m6A RNA binding protein immunoprecipitation assay was used to study how the m6A modification of ZKSCAN3 mRNA is regulated by ALKBH.RESULTS The increased expression of amylase and inflammatory factors in the supernatant and the accumulation of autophagic vacuoles verified that the AP mouse cell model was established.The downregulation of LAMP2 and upregulation of LC3-II/I and SQSTM1 demonstrated that autophagy was impaired in AP.The expression of ZKSCAN3 was upregulated in AP.Inhibition of ZKSCAN3 increased the expression of LAMP2 and decreased the expression of the inflammatory factors,LC3-II/I and SQSTM1.Furthermore,ALKBH5 was upregulated in AP.Knockdown of ALKBH5 downregulated ZKSCAN3 expression and restored decreased autophagic flux in AP.Notably,the bioinformatic analysis revealed 23 potential m6A modification sites on ZKSCAN3 mRNA.The m6A modification of ZKSCAN3 mRNA was significantly decreased in AP.Knockdown of ALKBH5 increased the modification of ZKSCAN3 mRNA,which confirmed that ALKBH5 upregulated ZKSCAN3 expression in a m6A-dependent manner.CONCLUSION ALKBH5 inhibits autophagic flux through m6A demethylation of ZKSCAN3 mRNA in AP,thereby aggravating the severity of the disease.

关键词： Acute pancreatitis Autophagy ZKSCAN3 N6-methyladenosine ALKBH5

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Research on blade tip clearance cavitation and turbulent kinetic energy characteristics of axial flow pump based on the partially-averaged Navier-Stokes model

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Journal of Hydrodynamics 2024年第1期36卷 184-201页

作者： Xiao-qi Jia shuai-kang Zhang Zu-chao zhu Key Laboratory of Fluid Transmission Technology of Zhejiang Province Zhejiang Sci-Tech UniversityHangzhou310018China

To reveal the cavitation forms of tip leakage vortex(TLV)of the axial flow pump and the flow mechanism of the flow field,this research adopts the partially-averaged Navier-Stokes(PANS)model to simulate the cavitation values of an axial flow pump,followed by experimental validation.The experimental result shows that compared with the shear stress transport(SST)k-ωmodel,the PANS model significantly reduces the eddy viscosity of the flow field to make the vortex structure clearer and allow the turbulence scale to be more robustly analyzed.The cavitation area within the axial flow pump mainly comprises of TLV cavitation,clearance cavitation and tip leakage flows combined effect of triangular cloud cavitation formed.The formation and development of cavitation are accompanied by the formation and evolution of vortex,and variations in vortex structure also generate and promote the development of cavitation.In addition,an in-depth analysis of the relationship between the turbulent kinetic energy(TKE)transport equation and cavitation patterns was also conducted,finding that the regions with relatively high TKE are mainly distributed around gas/liquid boundaries with serious cavitation and evident gas-liquid change.This phenomenon is mainly attributed to the combined effect of the pressure action term,stress diffusion term and TKE production term.

关键词： Cavitation axial flow pump tip leakage vortex(TLV) partially-averaged Navier-Stokes(PANS)model turbulent kinetic energy(TKE)

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Synthesis of a High-Efficiency Demulsifier and Study on Optimal Demulsification Conditions

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China Petroleum Processing & Petrochemical Technology 2024年第1期26卷 107-115页

作者： zhu shuai Zhang YingYu Gui Xia College of Chemical Engineering Nanjing Tech University30 Puzhunan RoadNanjing 211816China

Oil and water separation has always been a top priority in the oil industry.In this study,a series of hyperbranched fluorinated polyamine-amine polymers(HFPA1-5)were synthesized directly using an improved“one-pot method.”The highly active fluorinated p-trifluoromethylaniline was used as the core raw material,while diethylenetriamine and methyl acrylate were used as the chain segment.A hyperbranched fluorine-containing polyamine-amine demulsifier(NHFPA6)was obtained through nano-grafting copolymerization of HFPA5.To enhance the demulsification and dehydration performance,the copolymerized HFPA6 was modified and combined.Then,the effects of the combination ratio,demulsifier concentration,demulsification time,and demulsification temperature on the demulsification effect were investigated.The results revealed that a combination ratio of DE-401:NHFPA6=1:1,a demulsification temperature of 50℃,a demulsification time of 60 min,and a demulsifier concentration of 150 mg/L yielded a dehydration rate as high as 99.80%.A response surface optimization design of demulsification conditions was performed.The model verified that the optimal demulsification conditions were 50℃,300 mg/L,and 90 min.However,considering the economic benefits of factories,it is more favorable to select demulsification conditions with a shorter time and lower concentration when the dehydration standard is met.Therefore,the demulsification conditions were selected as 50℃,150 mg/L,and 60 min.Compared to existing demulsifiers,the demulsifier developed in this study exhibits a lower demulsification temperature and higher demulsification efficiency.

关键词： hyperbranched fluorinated polyamine-amine nano-SiO_(2)

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Synergistic catalysis of the N-hydroxyphthalimide on flower-like bimetallic metal-organic frameworks for boosting oxidative desulfurization

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Petroleum Science 2024年第1期21卷 674-682页

作者： Jing He Kun zhu Wei Jiang Dong-Ao zhu Lin-Hua zhu Hai-Yan Huang Wen-shuai zhu Hua-Ming Li Institute for Energy Research Jiangsu UniversityZhenjiang212013JiangsuChina College of Chemistry and Chemical Engineering Hainan Normal UniversityKey Laboratory of Water Pollution Treatment and Resource Reuse of Hainan ProvinceHaikou571158HainanChina

Synergic catalytic effect between active sites and supports greatly determines the catalytic activity for the aerobic oxidative desulfurization of fuel oils.In this work,Ni-doped Co-based bimetallic metal-organic framework(CoNi-MOF)is fabricated to disperse N-hydroxyphthalimide(NHPI),in which the whole catalyst provides plentiful synergic catalytic effect to improve the performance of oxidative desulfurization(ODS).As a bimetallic MOF,the second metal Ni doping results in the flower-like morphology and the modification of electronic properties,which ensure the exposure of NHPI and strengthen the synergistic effect of the overall catalyst.Compared with the monometallic Co-MOF and naked NHPI,the NHPI@CoNi-MOF triggers the efficient activation of molecular oxygen and improves the ODS performance without an initiator.The sulfur removal of dibenzothiophene-based model oil reaches 96.4%over the NHPI@CoNi-MOF catalyst in 8 h of reaction.Furthermore,the catalytic product of this aerobic ODS reaction is sulfone,which is adsorbed on the catalyst surface due to the difference in polarity.This work provides new insight and strategy for the design of a strong synergic catalytic effect between NHPI and bimetallic supports toward high-activity aerobic ODS materials.

关键词： Metal-organic frameworks Doped Bimetallic N-hydroxyphthalimide Aerobic processes Oxidative desulfurization

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Expression Complementary Disentanglement Network for Facial Expression Recognition

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Chinese Journal of Electronics 2024年第3期33卷 742-752页

作者： Shanmin WANG Hui shuai Lei zhu Qingshan LIU College of Computer Science and Technology Nanjing University of Aeronautics and Astronautics College of Computer and Software Nanjing University of Information Science and Technology School of Information and Control Nanjing University of Information Science and Technology

Disentangling facial expressions from other disturbing facial attributes in face images is an essential topic for facial expression recognition.Previous methods only care about facial expression disentanglement(FED)itself,ignoring the negative effects of other facial attributes.Due to the annotations on limited facial attributes,it is difficult for existing FED solutions to disentangle all disturbance from the input face.To solve this issue,we propose an expression complementary disentanglement network(ECDNet).ECDNet proposes to finish the FED task during a face reconstruction process,so as to address all facial attributes during disentanglement.Different from traditional reconstruction models,ECDNet reconstructs face images by progressively generating and combining facial appearance and matching geometry.It designs the expression incentive(EIE) and expression inhibition(EIN) mechanisms,inducing the model to characterize the disentangled expression and complementary parts precisely.Facial geometry and appearance,generated in the reconstructed process,are dealt with to represent facial expressions and complementary parts,respectively.The combination of distinctive reconstruction model,EIE,and EIN mechanisms ensures the completeness and exactness of the FED task.Experimental results on RAF-DB,AffectNet,and CAER-S datasets have proven the effectiveness and superiority of ECDNet.

关键词： Geometry Image recognition Annotations Face recognition Task analysis Image reconstruction Facial features

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Incidence,prevalence,and causes of spinal injuries in China,1990-2019:Findings from the Global Burden of Disease Study 2019

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Chinese Medical Journal 2024年第6期137卷 704-710页

作者： Chenjun Liu Tingling Xu Weiwei Xia shuai Xu Zhenqi zhu Maigeng Zhou Haiying Liu Department of Spinal Surgery Peking University People’s HospitalPeking UniversityBeijing 100044China Chinese Preventive Medicine Association Committee on Prevention and Control of Spinal DiseaseBeijing 100009China National Center for Chronic and Noncommunicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 100050China School of Public Health Xi’an Jiaotong UniversityXi’anShaanxi 710061China

Background:Spinal injuries are an urgent public health priority;nevertheless,no China-wide studies of these injuries exist.This study measured the incidence,prevalence,causes,regional distribution,and annual trends of spinal injuries in China from 1990 to 2019.Methods:We used data from the Global Burden of Diseases,Injuries,and Risk Factors Study 2019 to estimate the incidence and prevalence of spinal injuries in China.The data of 33 provincial-level administrative regions(excluding Taiwan,China)provided by the National Center for Chronic and Noncommunicable Disease Control and Prevention,Chinese Center for Disease Control and Prevention(CDC)were use to systematically analyze the provincial etiology,geographical distribution,and annual trends of spinal injuries.The Bayesian meta-regression tool DisMod-MR 2.1 was used to ensure the consistency among incidence,prevalence,and mortality rates in each case.Results:From 1990 to 2019,the number of living patients with spinal injuries in China increased by 138.32%,from 2.14 million to 5.10 million,while the corresponding age-standardized prevalence increased from 0.20%(95%uncertainty interval[UI]:0.18-0.21%)to 0.27%(95%UI:0.26-0.29%).The incidence of spinal injuries in China increased by 89.91%(95%UI:72.39-107.66%),and the prevalence increased by 98.20%(95%UI:89.56-106.82%),both the most significant increases among the G20 countries;71.00%of the increase could be explained by age-specific prevalence.In 2019,the incidence was 16.47(95%UI:12.08-22.00,per 100,000 population),and the prevalence was 358.30(95%UI:333.96-386.62,per 100,000 population).Based on the data of 33 provincial-level administrative regions provided by CDC,age-standardized incidence and prevalence were both highest in developed provinces in Eastern China.The primary causes were falls and road injuries;however,the prevalence and specific causes differed across provinces.Conclusions:In China,the overall disease burden of spinal injuries increased significantly during the past three decades but varied consi

关键词： Spinal injuries Global Burden of Diseases,Injuries and Risk Factors Study 2019 Incidence Prevalence Falls Road injuries

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Unresolved Excess Accumulation of Myelin-Derived Cholesterol Contributes to Scar Formation after Spinal Cord Injury

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Research 2024年第1期2023卷 17-32页

作者： Bolin Zheng Yijing He shuai Yin Xu zhu Qing Zhao Huiyi Yang Zhaojie wang Rongrong zhu Liming Cheng Key Laboratory of Spine and Spinal Cord Injury Repair and Regeneration Ministry of EducationDepartment of OrthopedicsTongji HospitalSchool of MedicineTongji UniversityShanghai 200092China Frontier Science Center for Stem Cell Research School of Life Science and TechnologyTongji UniversityShanghai 200092China Clinical Center for Brain and Spinal Cord Research Tongji UniversityShanghai 200092China

Spinal cord injury triggers complex pathological cascades,resulting in destructive tissue damage and incomplete tissue repair.Scar formation is generally considered a barrier for regeneration in the central nervous system.However,the intrinsic mechanism of scar formation after spinal cord injury has not been fully elucidated.Here,we report that excess cholesterol accumulates in phagocytes and is inefficiently removed from spinal cord lesions in young adult mice.Interestingly,we observed that excessive cholesterol also accumulates in injured peripheral nerves but is subsequently removed by reverse cholesteroltransport.Meanwhile,preventing reverse cholesterol transport leads to macrophage accumulation and fibrosis in injured peripheral nerves.Furthermore,the neonatal mouse spinal cord lesions are devoid of myelin-derived lipids and can heal without excess cholesterol accumulation.We found that transplantation of myelin into neonatal lesions disrupts healing with excessive cholesterol accumulation,persistent macrophage activation,and fibrosis.Myelin internalization suppresses macrophage apoptosis mediated by CD5L expression,indicating that myelin-derived cholesterol plays a critical role in impaired wound healing.Taken together,our data suggest that the central nervous system lacks an efficient approach for cholesterol clearance,resulting in excessive accumulation of myelin-derived cholesterol,thereby inducing scar formation after injury.

关键词： healing Spinal impaired

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Hepatic perivascular epithelioid cell tumors:The importance of preoperative diagnosis

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World Journal of Gastroenterology 2024年第13期30卷 1926-1933页

作者： shuai Yan Jia-Jie Lu Lin Chen Wei-Hua Cai Jin-zhu Wu Department of Medical School Nantong UniversityNantong 226300Jiangsu ProvinceChina Department of Hepatobiliary Surgery Affiliated Nantong Hospital 3 of Nantong UniversityNantong 226006Jiangsu ProvinceChina Nantong Institute of Liver Disease Affiliated Nantong Hospital 3 of Nantong UniversityNantong 226006Jiangsu ProvinceChina

Accurate preoperative diagnosis is highly important for the treatment of perivascular epithelioid cell tumors(PEComas)because PEComas are mainly benign tumors and may not require surgical intervention.By analyzing the causes,properties and clinical manifestations of PEComas,we summarize the challenges and solutions in the diagnosis of PEComas.

关键词： Hepatic perivascular epithelioid cell tumors Liver Preoperative diagnosis Angiomyolipomas Mesenchymal tissue-derived tumors

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Chemical profiling of principle active and toxic constituents in herbs containing aristolochic acids

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Chinese Herbal Medicines 2024年第2期16卷 293-300页

作者： Lijun Yue Kaijun Yang Feng Jiang shuai Dong Kang Yang Dan zhu School of Pharmacy Minzu University of ChinaBeijing 100081China Key Laboratory of Ethnomedicine Ministry of Education Minzu University of ChinaBeijing 100081China Hospital of Minzu University of China Beijing 100081China

Objective:To clear the amounts of the principal active/toxic components in herbs containing aristolochic acids(HCAAs),which are still used as medicine and/or seasoning in many ethnic minority areas of China.Methods:In this study,six major active and toxic components in HCAAs were extracted with ultrasonic extraction.With 6-O-methyl guanosine as internal standard,the target compounds were analyzed qualitatively and quantitatively by using ultrahigh performance liquid chromatography-electrospray ionization-tandem mass spectrometry(UPLC-ESI-MS/MS)with multiple reaction monitoringinformation dependent acquisition-enhanced production ion scanning mode(MRM-IDA-EPI)combined with dynamic background subtraction(DBS)function.Results:The method showed good linearity in the linear range of the six analytes.The limit range of detection was from 0.01 ng/mL to 0.27 ng/mL.All of the detection repeatability,extraction repeatability and accuracy of the method were good.After extraction,the samples remained stable at 15℃ within 24 h.Six analytes were all found in samples except aristolactam(AL)in sample 2,and the contents varied greatly.The contents of these compounds decreased in fruits,leaves and stems of Aristolochia delavayi successively.Conclusion:This method has the advantages of less sample dosage,simple operation,short analysis cycle,high sensitivity,specificity and accuracy.It laid a good foundation for guiding the safety of HCAAs,the indepth study of pharmacological and toxicological effects and the scientific and standardized processing and compatibility of HCAAs.

关键词： aristolactam aristolochic acids chemical profiling herbs containing aristolochic acids ultrahigh performance liquid chromatography-electrospray ionizationtandem mass spectrometry(UPLC-ESI-MS/MS)

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FUSE: a federated learning and U-shape split learning-based electricity theft detection framework

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Science China(Information Sciences) 2024年第4期67卷 339-340页

作者： Xuan LI Naiyu WANG Liehuang zhu shuai YUAN Zhitao GUAN School of Control and Computer Engineering North China Electric Power University School of Cyberspace Science and Technology Beijing Institute of Technology Department of Finance Operationsand Information Systems(FOIS)Brock University

In recent years, the data-driven electricity theft detection methods integrated with edge cloud computing [1, 2] have not only demonstrated superior detection accuracy but also improved efficiency, making them viable alternatives to indoor inspections. Energy service providers(ESPs) typically manage regions by dividing them into various transformer districts(TDs). The detection of electricity theft in a particular region is performed by the associated TD,

关键词： Sensitive data

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