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Maternal stress and the early embryonic microenvironment:investigating long‑term cortisol effects on bovine oviductal epithelial cells using air–liquid interface culture

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Journal of Animal Science and Biotechnology 2025年第1期16卷 153-166页

作者： Fiona Wahl Jianchao Huo shuaizhi Du Jennifer Schoen shuai chen Department of Reproduction Biology Leibniz Institute for Zoo and Wildlife Research(IZW)Alfred‑Kowalke‑Straße 1710315 BerlinGermany Institute of Biotechnology Technische Universität BerlinStraße Des 17.Juni 13510623 BerlinGermany Institute of Reproductive Biology Research Institute for Farm Animal Biology(FBN)Wilhelm‑Stahl‑Allee 218196 DummerstorfGermany

The oviduct epithelium is the initial maternal contact site for embryos after fertilization,offering the microenviron-ment before *** early gestation period is particularly sensitive to stress,which can cause reduced fertil-ity and reproductive disorders in ***,the local impact of elevated stress hormones on the ovi-duct epithelium has received limited attention to date,except for a few reports on polyovulatory species like mice and *** this study,we focused on the effects of chronic maternal stress on cattle,given its association with infertil-ity issues in this monoovulatory *** oviduct epithelial cells(BOEC)differentiated at the air–liquid interface(ALI)were stimulated with 250 nmol/L cortisol for 1 or 3 ***,they were assessed for morphology,bioelectrical properties,and gene expression related to oviduct function,glucocorticoid pathway,cortisol metabo-lism,inflammation,and *** revealed adverse effects of cortisol on epithelium structure,featured by deciliation,vacuole formation,and ***,cortisol exposure led to an increase in transepithelial potential difference,downregulated mRNA expression of the major glucocorticoid receptor(NR3C1),upregulated the expression of cortisol-responsive genes(FKBP5,TSC22D3),and significant downregulation of oviductal glycopro-tein 1(OVGP1)and steroid receptors PGR and *** systematic comparison to a similar experiment previously performed by us in porcine oviduct epithelial cells,indicated that bovine cultures were more susceptible to elevated cortisol levels than *** distinct responses between both species are likely linked to their divergence in the cortisol-induced expression changes of HSD11B2,an enzyme controlling the cellular capacity to metabolise *** findings provide insights into the species-specific reactions and reproductive consequences triggered by maternal stress.

关键词： Air–liquid interface Bovine Cortisol Early embryonic microenvironment Epithelial cells Maternal stress Oviduct

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Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan shuai chen Dan Liu Qian Xiao Jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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Alkali-extracted polysaccharides from mung bean skin possess protective effect on H_(2)O_(2)-induced IEC-6 cells:the role of NF-κB and MAPK signaling pathways

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Food Science and Human Wellness 2025年第1期14卷 101-111页

作者： shuai chen Liuming Xie Pingwei Wen Qiang Yu Yi chen Hanyu Lu Ting chen Xin Tao Jianhua Xie State Key Laboratory of Food Science and Resources Nanchang UniversityNanchang 330047China International Institute of Food Innovation Co. Ltd.Nanchang UniversityNanchang 330200China

Intestinal oxidation-reduction balance is essential to protect host *** on the underlying mechanisms of oxidation homeostasis can provide conditions to the treatment or protection of intestinal oxidative ***,the mechanism of polysaccharide(MBP)with alkali-extracted from mung bean skin in alleviating oxidative damage induced by H_(2)O_(2)-induced in intestinal epithelial cells(IEC)-6 cells was *** indicated that MBP effectively mitigated the H_(2)O_(2)-induced decrease in IEC-6 cell viability,restored superoxide dismutase(SOD)activity,lowered malondialdehyde(MDA)content,and decreased reactive oxygen species(ROS)*** blotting showed that MBP may mitigate oxidative damage through NF-κB,MAPK and Nrf2 signaling pathway,and RNA sequencing(RNA-seq)analysis confirmed that MBP can protect cells by regulating MAPK and NF-κB signaling ***,the results of the inhibitor experiment also support the above *** results verified that MBP has the capacity to mitigate oxidative damage induced by H_(2)O_(2)in IEC-6 cells through MAPK and NF-κB signaling *** study showed that MBP can assist preventing intestinal diseases relating to oxidative stress,which could facilitate the development of functional foods.

关键词： Polysaccharide Alkali-extracted Oxidative stress RNA sequencing Molecular mechanism

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Targeting IGF1 to alleviate obesity through regulating energy expenditure and fat deposition

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Science China Life Sciences 2025年

作者： Ping Rong Yinqiu Mu Meiqin Wang Liang chen Fangtong Liu Yuxin Jin Weikuan Feng Kun Zhou Hui Liang Hongyu Wang shuai chen State Key Laboratory of Pharmaceutical Biotechnology Department of Endocrinology Nanjing Drum Tower Hospital The Affiliated Hospital of Nanjing University Medical School Model Animal Research Center Nanjing University MOE Key Laboratory of Model Animal for Disease Study Model Animal Research Center School of Medicine Nanjing University College of Life Sciences Anhui Medical University Department of General Surgery First Affiliated Hospital Nanjing Medical University

Insulin-like growth factor 1 (IGF1) is a regulator of both cellular hypertrophy and lipogenesis,which are two key processes for pathogenesis of ***,the in vivo role of IGF1 in the development of obesity remains ***,we show that IGF1 expression is increased in adipose tissue in obese human patients and animal *** of IGF1 is associated with increased lipogenic gene expression and decreased energy *** down-regulation of IGF1 normalizes lipogenic gene expression,restores aberrant energy metabolism and alleviates obese phenotype of a genetic mouse model with ***,genetic down-regulation of IGF1 exerts similar effects on development of diet-induced ***,berberine that is an AMP-activated protein kinase (AMPK) activator in medicinal herbs inhibits IGF1 secretion,decreases lipogenic gene expression and alleviates diet-induced ***,our findings demonstrate that hypersecretion of IGF1 is a critical factor for the development of obesity and can be targeted using AMPK activators to alleviate obesity.

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Si对奥氏体不锈钢应力腐蚀开裂性能的影响

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稀有金属材料与工程 2025年第1期54卷 109-117页

作者：公维佳陈帅刘禄鹏杜东海西北工业大学太仓长三角研究院/清洁能源研究院江苏太仓215400 中船黄埔文冲船舶有限公司广东广州510715

堆芯内奥氏体不锈钢的辐照促进应力腐蚀开裂(IASCC)是影响核反应堆服役安全和寿命的重要影响因素之一。大量的研究表明,奥氏体不锈钢的IASCC与辐照引起的Si在晶界富集有关。为探索晶界Si富集程度对奥氏体不锈钢应力腐蚀开裂速率的影响,... 详细信息

堆芯内奥氏体不锈钢的辐照促进应力腐蚀开裂(IASCC)是影响核反应堆服役安全和寿命的重要影响因素之一。大量的研究表明,奥氏体不锈钢的IASCC与辐照引起的Si在晶界富集有关。为探索晶界Si富集程度对奥氏体不锈钢应力腐蚀开裂速率的影响,测量了Si含量不同的模型合金在320℃高温水中的裂纹扩展速率(CGR),深入分析了不同合金的裂纹扩展路径和断口形貌特征。研究发现,在含氧水环境中,含Si合金的CGR较高且对Si含量无明显依赖关系;而在含氢水化学环境中,随着Si含量的增加,CGR增加并达到在含氧水环境中的水平。应力腐蚀裂纹主要沿着大角度晶界扩展,而且Si含量越高,样品的裂纹尖端就越尖锐。晶界富集的Si在高温水中被氧化,生成的氧化膜易溶于水,导致晶界氧化膜的强度降低,在应力的作用下更容易开裂,引起应力腐蚀开裂敏感性上升。

关键词：奥氏体不锈钢 Si偏析应力腐蚀裂纹扩展速率

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g-C_(3)N_(4)基异质结光还原CO_(2)的研究进展

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燃料化学学报(中英文) 2025年第1期53卷 40-52页

作者：任富彦孙振马涛张浩卫萌陈帅新疆科技学院新疆库尔勒841000

光催化技术能够将CO_(2)转化为有价值的烃类化合物,为解决化石燃料短缺和全球变暖问题提供了新的途径。然而,传统半导体光催化剂由于比表面积小和吸附CO_(2)能力不足,效果有限。g-C_(3)N_(4)凭借其无毒、高稳定性和低成本特性,在光催化... 详细信息

光催化技术能够将CO_(2)转化为有价值的烃类化合物,为解决化石燃料短缺和全球变暖问题提供了新的途径。然而,传统半导体光催化剂由于比表面积小和吸附CO_(2)能力不足,效果有限。g-C_(3)N_(4)凭借其无毒、高稳定性和低成本特性,在光催化领域备受关注。尽管纯g-C_(3)N_(4)的光催化效率受到光生电子/空穴对快速复合、比表面积小和光吸收不足的制约,但通过与大带隙半导体形成异质结构,g-C_(3)N_(4)的电荷分离、比表面积和光吸收能力得到了显著增强。这种基于g-C_(3)N_(4)的异质结构包括半导体支持型、炭材料支持型、非金属支持型以及金属有机骨架支持型,它们在CO_(2)光转换中展现出巨大潜力。然而,改性g-C_(3)N_(4)基异质结构在CO_(2)光转换中仍面临挑战,需要进一步的研究和设计创新。这篇综述强调了基于g-C_(3)N_(4)的异质结构在环保且可持续的CO_(2)还原方法中的重要作用。

关键词： g-C_(3)N_(4) 光还原CO_(2) 异质结半导体材料金属有机框架

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基于波形匹配的柔性直流输电线路单端量保护研究

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电力系统保护与控制 2025年第3期53卷 13-26页

作者：贾科刘可歆毕天姝姚昆鹏陈帅李再男新能源电力系统国家重点实验室(华北电力大学) 北京102206

柔性直流输电技术是解决大规模新能源接入问题的有力抓手,其线路保护是保障系统安全稳定的关键。线路单端量保护不需要借助通信,能较好地满足柔性直流输电系统对时间的要求。然而现有柔性直流输电系统单端量保护性能受后续行波影响较大... 详细信息

柔性直流输电技术是解决大规模新能源接入问题的有力抓手,其线路保护是保障系统安全稳定的关键。线路单端量保护不需要借助通信,能较好地满足柔性直流输电系统对时间的要求。然而现有柔性直流输电系统单端量保护性能受后续行波影响较大,存在一定范围保护死区。对此,在充分考虑后续行波对首行波影响的基础上,深度挖掘区内外故障行波差异,进而提出基于波形匹配的故障识别判据。该判据可通过匹配误差与匹配波形特征相结合衡量后续行波影响程度,最终实现全线速动保护。基于PSCAD/EMTDC搭建了四端柔性直流输电系统。仿真结果验证了所提方法的可行性,且具有保护范围长、耐受过渡电阻能力强、抗噪声干扰能力强的优势。

关键词：柔性直流输电后续行波波形匹配波形特征单端量保护

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热塑性纤维素与PVA混溶性的分子动力学模拟

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原子与分子物理学报 2025年第3期42卷 149-158页

作者：冯彦洪陈帅杰孙航华南理工大学机械与汽车工程学院聚合物新型成型装备国家工程研究中心聚合物成型加工工程教育部重点实验室广东省高分子先进制造技术及装备重点实验室广州510000

首次提出了将氧化还原改性纤维素(Oxidation-reduction modified cellulose,ORC)与聚乙烯醇(Poly(vinyl alcohol),PVA)共混进行制备复合材料,并采用分子动力学模拟研究了ORC与PVA的混溶性.对分子链间相互作用能和氢键数量分析发现,随着... 详细信息

首次提出了将氧化还原改性纤维素(Oxidation-reduction modified cellulose,ORC)与聚乙烯醇(Poly(vinyl alcohol),PVA)共混进行制备复合材料,并采用分子动力学模拟研究了ORC与PVA的混溶性.对分子链间相互作用能和氢键数量分析发现,随着改性程度增加,ORC与PVA会产生更强烈的相互作用,这促进了组分间的混溶.当ORC的改性程度达到40%后,ORC与PVA的溶解度参数差值为2.02(J/cm^(3))^(1/2),达到混溶条件.根据Flory-Huggins理论确定了醇解度为88%和99%的PVA与ORC的混溶性,结果表明PVA(88%)与ORC的混溶性更好.对共混体系中形成氢键原子的径向分布函数分析发现,醋酸乙烯酯重复单元上的羰基更倾向与ORC形成分子链间氢键,而乙烯醇重复单元上的羟基更倾向形成分子链内氢键,这促使PVA(88%)/ORC共混体系中形成更多的分子链间氢键,从而混溶性更好.研究结果可为ORC/PVA复合材料的制备提供理论指导.

关键词：混溶性氧化还原改性纤维素分子动力学聚乙烯醇

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A dynamic PUF anti-aging authentication system based on restrict race code

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Science China(Information Sciences) 2016年第1期59卷 149-160页

作者： Bing LI shuai chen Southeast University School of Integrated Circuit

This paper presents a PUF(physical unclonable functions) authentication mechanism which can combat aging based on restrict race code(RRC). PUF provide a mechanism for identifying an integrated circuit based on the intrinsic variations of physical components uniquely and provide a cheap and secure alternative to random message storage on devices. However, due to its own characteristics, the chip-level PUF circuit reliability is easily affected by environment. The effect of reversible temporal noise on PUF is well studied and has obtained good effect(e.g. temperature, voltage), but sufficient attention has not been given so far to analyze the effect of the irreversible temporal variabilities(e.g. aging on PUF). This paper proposes a dynamic PUF authentication method, which is based on the RRC with two-ring strong characteristics structure. A combination of hardware and software system is used to improve the efficiency of authentication, and in an FPGA(field-programmable gate array)-based SRAM(static random access memory) PUF are used as an experimental object to verify the feasibility of this design. And this system can enhance the authentication system safety from the CVC(characteristic value challenge) randomness test.

关键词： aging PUF dynamically maintain RRC characteristic value

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New scoring system for the evaluation obstructive degrees based on computed tomography for obstructive colorectal cancer

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World Journal of Gastrointestinal Oncology 2025年第3期17.0卷 122-132页

作者： Shang-Guan, Xin-Chang Zhang, Jun-Rong Lin, Chao-Nan chen, shuai Wei, Yong chen, Wen-Xuan Pan, Lin Huang, Li-Qin Zheng, Shao-Hua chen, Xian-Qiang Department of Emergency Surgery Fujian Medical University Union Hospital Fujian Province Fuzhou 350001 China College of Physics and Information Engineering Fuzhou University Fujian Province Fuzhou 350001 China Department of Emergency Surgery Fujian Medical University Union Hospital No. 29 Xinquan Road Fujian Province Fuzhou 350001 China

BACKGROUND The degree of obstruction plays an important role in decision-making for obstructive colorectal cancer (OCRC). The existing assessment still relies on the colorectal obstruction scoring system (CROSS) which is based on a comprehensive analysis of patients’ complaints and eating conditions. The data collection relies on subjective descriptions and lacks objective parameters. Therefore, a scoring system for the evaluation of computed tomography-based obstructive degree (CTOD) is urgently required for OCRC. AIM To explore the relationship between CTOD and CROSS and to determine whether CTOD could affect the short-term and long-term prognosis. METHODS Of 173 patients were enrolled. CTOD was obtained using k-means, the ratio of proximal to distal obstruction, and the proportion of nonparenchymal areas at the site of obstruction. CTOD was integrated with the CROSS to analyze the effect of emergency intervention on complications. Short-term and long-term outcomes were compared between the groups. RESULTS CTOD severe obstruction (CTOD grade 3) was an independent risk factor [odds ratio (OR) = 3.390, 95% confidence interval (CI): 1.340–8.570, P = 0.010] via multivariate analysis of short-term outcomes, while CROSS grade was not. In the CTOD-CROSS grade system, for the non-severe obstructive (CTOD 1–2 to CROSS 1–4) group, the complication rate of emergency interventions was significantly higher than that of non-emergency interventions (71.4% vs 41.8%, P = 0.040). The postoperative pneumonia rate was higher in the emergency intervention group than in the non-severe obstructive group (35.7% vs 8.9%, P = 0.020). However, CTOD grade was not an independent risk factor of overall survival and progression-free survival. CONCLUSION CTOD was useful in preoperative decision-making to avoid unnecessary emergency interventions and complications. © The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.

关键词： Colorectal obstruction scoring system Computed tomography-based obstructive degree Emergency intervention Obstructive colorectal cancer Scoring system

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