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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第1期11卷 30-40页

作者： Jian liu Meng LI Jun-min LI Chang-jun HUANG Xue-ping ZHOU Fang-cheng XU shu-sheng liu Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogens and Insects Institute of Insect Sciences Zhejiang University Hangzhou 310029 China) Institute of Biotechnology Zhejiang University Hangzhou 310029 China Wenzhou Academy of Agricultural Sciences Wenzhou 325006 China

The ecological effects of plant-virus-vector interactions on invasion of alien plant viral vectors have been rarely investigated. We examined the transmission of Tomato yellow leaf cur/China virus （TYLCCNV） by the invasive Q biotype and the indigenous ZHJ2 biotype of the whitefly Bemisia tabaci, a plant viral vector, as well as the influence of TYLCCNV-infection of plants on the performance of the two whitefly biotypes. Both whitefly biotypes were able to acquire viruses from infected plants and retained them in their bodies, but were unable to transmit them to either tobacco or tomato plants. However, when the Q biotype fed on tobacco plants infected with TYLCCNV, its fecundity and longevity were increased by 7- and 1-fold, respectively, compared to those of the Q biotype fed on uninfected tobacco plants. When the ZHJ2 biotype fed on virus-infected plants, its fecundity and longevity were increased by only 2- and 0.5-fold, respectively. These data show that the Q biotype acquired higher beneficial effects from TYLCCNV-infection of tobacco plants than the ZHJ2 biotype. Thus, the Q biotype whitefly may have advantages in its invasion and displacement of the indigenous ZHJ2 biotype.

关键词： Bemisia tabaci Biotypes Begomovirus Vector-virus interaction Biological invasion

Sequencing and comparison of the Rickettsia genomes from the whitefly Bemisia tabaci Middle East Asia Minor I

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Insect Science 2016年第4期23卷 531-542页

作者： Dan-Tong Zhu Wen-Qiang Xia Qiong Rao shu-sheng liu Murad Ghanim Xiao-Wei Wang Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou China Department of Entomology Agricultural Research Organization The Volcani Center Bet Dagan Israel School of Agriculture and Food Science Zhejiang Agriculture and Forestry University Lin'an Hangzhou China

The whitefly, Bemisia tabaci, harbors the primary symbiont ＇Candidatus Portiera aleyrodidarum＇ and a variety of secondary symbionts. Among these secondary symbionts, Rickettsia is the only one that can be detected both inside and outside the bacteriomes. Infection with Rickettsia has been reported to influence several aspects of the whitefly biology, such as fitness, sex ratio, virus transmission and resistance to pesticides. However, mechanisms underlying these differences remain unclear, largely due to the lack of genomic information of Rickettsia. In this study, we sequenced the genome of two Rickettsia strains isolated from the Middle East Asia Minor 1 （MEAM1） species of the B. tabaci complex in China and Israel. Both Rickettsia genomes were of high coding den- sity and AT-rich, containing more than 1000 coding sequences, much larger than that of the coexisted primary symbiont, Portiera. Moreover, the two Rickettsia strains isolated from China and Israel shared most of the genes with 100% identity and only nine genes showed sequence differences. The phylogenetic analysis using orthologs shared in the genus, inferred the proximity of Rickettsia in MEAM1 and Rickettsia bellii. Functional analysis revealed that Rickettsia was unable to synthesize amino acids required for complementing the whitefly nutrition. Besides, a type IV secretion system and a number of virulence- related genes were detected in the Rickettsia genome. The presence of virulence-related genes might benefit the symbiotic life of the bacteria, and hint on potential effects of Rickettsia on whiteflies. The genome sequences of Rickettsia provided a basis for further understanding the function of Rickettsia in whiteflies.

关键词： Bemisia tabaci comparison endosymbiont genome Rickettsia symbiosis

Genome-wide identification and characterization of HSP gene superfamily in whitefly (Bemisia tabacl)and expression profiling analysis under temperature stress

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Insect Science 2019年第1期26卷 44-57页

作者： Xin-Ru Wang Chao Wang Fei-Xue Ban Dan-Tong Zhu shu-sheng liu Xiao-Wei Wang Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect SciencesZhejiang UniversityHangzhouChina

Heat shock proteins (HSP)are essential molecular chaperones that play important roles in the stress stimulation of *** tabaci,a phloem feeder and invasive species,can cause extensive crop damage through direct feeding and transmission of plant *** we employed comprehensive genomics approaches to identity HSP superfamily members in the Middle East Asia Minor 1 whitefly *** total,we identified 26 Hsp genes,including three Hsp90,17 Hsp70,one Hsp60 and five sHSP (small heat shock protein)*** HSP gene superfamily of whitefly is expanded compared with the other five insects surveyed *** gene structures among the same families are relatively ***,the motif compositions and secondary structures of BtHsp proteins were *** addition,quantitative polymerase chain reaction analysis showed that the expression patterns of BtHsp gene superfamily were diverse across different tissues of *** Hsp genes were induced or repressed by thermal stress (40℃)and cold treatment (4℃)in *** the expression of BtHsp70-6 significantly decreased the survival rate of whitefly under 45℃.All the results showed the Hsps conferred thermo-tolerance or cold-tolerance to whiteflies that protect them from being affected by detrimental temperature *** observations highlighted the molecular evolutionary properties and the response mechanism to temperature assaults of Hsp genes in whitefly.

关键词： cold stress comparative genomics HSP phylogenetic thermal stress whitefly

Temporal changes of symbiont density and host fitness after rifampicin treatment in a whitefly of the Bemisia tabaci species complex

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Insect Science 2016年第2期23卷 200-214页

作者： Hong-Wei Shan Chang-Rong Zhang Ting-Ting Yan Hai-Qin Tang Xiao-Wei Wang shu-sheng liu Yin-Quan liu Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou China

Microbial symbionts are essential or important partners to phloem-feeding insects. Antibiotics have been used to selectively eliminate symbionts from their host insects and establish host lines with or without certain symbionts for investigating functions of the symbionts. In this study, using the antibiotic rifampicin we attempted to selectively eliminate certain symbionts from a population of the Middle East-Asia Minor 1 whitefly of the Bemisia tabaci species complex, which harbors the primary symbiont ＂Candidatus Portiera aleyrodidarum＂ and two secondary symbionts ＂Candidatus Hamiltonella defensa＂ and Rickettsia. Neither the primary nor the secondary symbionts were completely depleted in the adults （F0） that fed for 48 h on a diet treated with rifampicin at concentrations of 1 100/zg/mL. However, both the primary and secondary symbionts were nearly completely depleted in the offspring （F 1） of the rifampicin-treated adults. Although the F1 adults produced some eggs （F2）, most of the eggs failed to hatch and none of them reached the second instar, and consequently the rifampicin-treated whitefly colony vanished at the F2 generation. Interestingly, quantitative polymerase chain reaction assays showed that in the rifampicin-treated whiteflies, the density of the primary symbiont was reduced at an obviously slower pace than the secondary symbionts. Mating experiments between rifampicin-treated and untreated adults demonstrated that the negative effects ofrifampicin on host fitness were expressed when the females were treated by the antibiotic, and whether males were treated or not by the antibiotic had little contribution to the negative effects. These observations indicate that with this whitefly population it is not feasible to selectively eliminate the secondary symbionts using rifampicin without affecting the primary symbiont and establish host lines for experimental studies. However, the extinction of the whitefly colony at the second generation after rifampicin

关键词： antibiotic primary symbiont secondary symbiont selective elimination ofsymbiont whitefly

Cloning of a putative extracellular Cu/Zn superoxide dismutase and functional differences of superoxide dismutases in invasive and indigenous whiteflies

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Insect Science 2015年第1期22卷 52-64页

作者： Xian-Long Gao Jun-Min Li Hong-Xing Xu Gen-Hong Yan Min Jiu shu-sheng liu Xiao-Wei Wang Ministry of Agriculture Key Laboratory of Agricultural Entomology Institute of Insect Sciences Zhejiang University Hangzhou Instituteof Virology and Biotechnology Zhejiang Academy of Agricultural Sciences Hangzhou College of Food and Bioengineering HenanUniversity of Science and Technology Luoyang China

Superoxide dismutases （SODs） are a group of important antioxidant defense enzymes. In this study, a putative extracellular Cu/Zn superoxide dismutase （ecCuZnSOD） complementary DNA was cloned and characterized from the whitefly, Bemisia tabaci. Quantitative polymerase chain reaction analysis showed that the expression level of BtecCuZnSOD was more than 10-fold higher in the invasive Middle East Asia Minor 1 （MEAM1） than in the native Asia II 3 species of the B. tabaci species complex. After exposure to low temperature （4 ℃）, the expression of Bt-ecCuZnSOD gene was significantly up-regulated in MEAM1 but not in Asia II 3. Furthermore, the expression level ofB. tabaci intracellular CuZnSOD （Bt-icCuZnSOD）, Bt-ecCuZnSOD and mitochondrial MnSOD （Bt-mMnSOD） was compared after transferring MEAM1 and Asia II 3 whiteflies from favorable （cotton） to unfavorable host plants （tobacco）. On cotton, both CuZnSOD genes were expressed at a higher level in MEAM1 compared with Asia II 3. Interestingly, after transferring onto tobacco, the expression of Bt-ecCuZnSOD was significantly induced in Asia II 3 but not in MEAM1. On the other hand, while Bt-mMnSOD was expressed equally in both species on cotton, Bt-mMnSOD messenger RNA was up-regulated in MEAM 1 on tobacco. Consistently, enzymatic activity assays of CuZnSOD and MnSOD demonstrated that CuZnSOD might play an important protective role against oxidative stress in Asia II 3, whereas MnSOD activation was critical for MEAM1 whiteflies during host adaptation. Taken together, our results suggest that the successful invasion ofMEAM 1 is correlated with its constitutive high activity of CuZnSOD and inducible expression of MnSOD under stress conditions.

关键词： Bemisia tabaci extracellular Cu/Zn superoxide dismutase oxidative strees superoxide dismutase whitefly

Enhancing cellular behavior in repaired tissue via silk fibroin-integrated triboelectric nanogenerators

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Microsystems & Nanoengineering 2024年第3期10卷 137-147页

作者： Zhelin Li shuxing Xu Zijie Xu sheng shu Guanlin liu Jianda Zhou Ding Lin Wei Tang Changsha Aier Eye Hospital Aier School of OphthalmologyCentral South UniversityChangshaHunanChina The Xiangya Hospital Central South UniversityChangshaHunanChina Beijing Institute of Nanoenergy and Nanosystems Chinese Academy of SciencesBeijing 101400China Center on Nanoenergy Research School of Physical Science&TechnologyGuangxi UniversityNanning 530004China School of Nanoscience and Technology University of Chinese Academy of SciencesBeijing 100049China Department of Plastic Surgery the Third Xiangya HospitalChangshaHunanChina

Triboelectric nanogenerators(TENGs)have emerged as a promising approach for generating electricity and providing electrical stimuli in medical electronic *** their potential benefits,the clinical implementation of TENGs faces challenges such as skin compliance and a lack of comprehensive assessment regarding their biosafety and ***,further research is imperative to overcome these limitations and unlock the full potential of TENGs in various biomedical *** this study,we present a flexible silk fibroin-based triboelectric nanogenerator(SFB-TENG)that features an on-skin substrate and is characterized by excellent skin compliance and air/water *** range of electrical output generated by the SFB-TENG was shown to facilitate the migration and proliferation of Hy926,NIH-3T3 and RSC96 ***,apoptosis of fibroblast NIH-3T3 cells was observed when the output voltage increased to more than 20 V at a frequency of 2 *** addition,the moderate electrical stimulation provided by the SFB-TENG promoted the cell proliferation cycle in Hy926 *** research highlights the efficacy of a TENG system featuring a flexible and skin-friendly design,as well as its safe operating conditions for use in biomedical *** findings position TENGs as highly promising candidates for practical applications in the field of tissue regeneration.

关键词： overcome spite generating

Fe/S oxidation-coupled arsenic speciation transformation mediated by AMD enrichment culture under different pH conditions

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Journal of Environmental Sciences 2024年第3期137卷 681-700页

作者： Yu-hang Zhou Wei-xi Huang Zhen-yuan Nie Hong-chang liu Yue liu Can Wang Jin-lan Xia Wen-sheng shu Key Lab of Biometallurgy of Ministry of Education of China School of Minerals Processing and BioengineeringCentral South UniversityChangsha 410083China School of Life Sciences South China Normal UniversityGuangzhou 510631China

Arsenic(As)speciation transformation in acid mine drainage(AMD)is comprehensively affected by biological and abiotic factors,such as microbially mediated Fe/S redox reactions and changes in environmental conditions(pH and oxidation-reduction potential).However,their combined impacts on arsenic speciation transformation remain poorly ***,we explored arsenic transformation and immobilization during pyrite dissolution mediated by AMD enrichment culture under different acidic pH *** results for incubation and mineralogical transformation of solid residues show that in the presence of AMD enrichment culture,pH 2.0,2.5,and 3.0 are more conducive to the formation of jarosites and ferric arsenate,which could immobilize high quantities of dissolved arsenic by adsorption and *** pH conditions significantly affect the initial adsorption of microbial cells to the minerals and the evolution of microbial community structure,further infuencing the biodissolution of pyrite and the release and oxidation process of Fe/*** results of Fe/S/As speciation transformation of the solid residues show that the transformation of Fe,S,and As in solution is mainly regulated by pH and potential values,which imposed significantly different effects on the formation of secondary minerals and thus arsenic oxidation and *** above results indicated that arsenic transformation is closely related to the Fe/S oxidation associated with pyrite bio-oxidation,and this correlation is critically regulated by the pH conditions of the system.

关键词： Acid mine drainage(AMD) pH conditions Fe/S/As speciation transformation Microbial community structure

A NOVEL METHOD FOR STUDYING THE EFFECT OF TEMPERATURE ARIATIONS ON THE DEVELOPMENT RATE OF INSECTS

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A NOVEL METHOD FOR STUDYING THE EFFECT OF TEMPERATURE ARIATI...

Cassiterite U-Pb age in the Bawapin tin deposit, Tanintharyi Region, southern Myanmar

全国昆虫生态学学术研讨会

作者： shu-sheng liu Department of Plant Protection Zhejiang Agricultural University

In statistical analysis of the effect of variable temperatures upon rate of development in insects,two separate reasons,i.e.,nonlinearity in the temperature-development rate relationship and the effect imposed by temperature variation per se,must be distinguished from each *** paper briefly reviewed the methods that have been used and pointed out the problems in this kind of analysis,and then developed a new *** new method took into account both the nonlinearity of the rate curve and the universal inherent variation in development rate among individuals in a population,yet imposed no assumption about the inherent *** method is simple to use and can reveal with certainty any effect of temperature variation upon rate of development in insects.

关键词： development rate variable temperature temperature variation

来源： cnki会议评论

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China Geology 2023年第2期6卷 358-360,I0046页

作者： Song-yang Wu shu-sheng liu Yong-fei Yang Bin Zhang Hui-min Liang Hong Zhang Xiang-ting Zeng Fei Nie Chengdu Center China Geological SurveyChengdu 610081China Applied Geological Survey Center China Geological SurveyChengdu 610036China

*** Southeast Asia has a 3500 km tin metallogenic belt in the world,extending from southern Yunnan Province to Bangka,*** region is also the world’s largest one regarding tin production,which accounts for 85%of historical tin production globally(Lehmann B,2021).Myanmar is one of the main tin producers within this region(33000 t in 2020)and has become the key supplier after China and *** on tin mineralization in Myanmar mostly focused on ore-related granites,for zircon geochronology,magma evolution,and tectonic settings(Mao W et al.,2020).

关键词： Myanmar mostly globally

Structure Regulation of Electric Double Layer via Hydrogen Bonding Effect to Realize High-Stability Lithium-Metal Batteries

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Energy & Environmental Materials 2024年第3期7卷 72-83页

作者： sheng liu Chaozhu shu Yu Yan Dayue Du Longfei Ren Ting Zeng Xiaojuan Wen Haoyang Xu Xinxiang Wang Guilei Tian Ying Zeng College of Materials and Chemistry&Chemical Engineering Chengdu University of Technology1#DongsanluErxianqiaoChengdu 610059China Institute of Fundamental and Frontier Sciences University of Electronic Science and Technology of ChinaChengdu 611731China State Key Laboratory of Polymer Materials Engineering Polymer Research InstituteSichuan UniversityChengdu 610065China

The interfacial chemistry of solid electrolyte interphases(SEI)on lithium(Li)electrode is directly determined by the structural chemistry of the electric double layer(EDL)at the ***,a strategy for regulating the structural chemistry of EDL via the introduction of intermolecular hydrogen bonds has been proposed(p-hydroxybenzoic acid(pHA)is selected as proof-of-concept).According to the molecular dynamics(MD)simulation and density functional theory(DFT)calculation results,the existence of hydrogen bonds realizes the anion structural rearrangement in the EDL,reduces the lowest unoccupied molecular orbital(LUMO)energy level of anions in the EDL,and the number of free solvent molecules,which promotes the formation of inorganic species-enriched SEI and eventually achieves the dendrite-free Li *** on this strategy,Li‖Cu cells can stably run over 185 cycles with an accumulated active Li loss of only 2.27 mAh cm^(-2),and the long-term cycle stability of Li‖Li cells is increased to 1200 *** addition,the full cell pairing with the commercial LiFePO_(4)(LFP)cathodes exhibits stable cycling performance at 1C,with a capacity retention close to 90%after 200 cycles.

关键词： electric double layer electrolyte additives intermolecular hydrogen bonds Li metal batteries p-Hydroxybenzoic acid