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Single-particle detection of cholesterol based on the host-guest recognition induced plasmon resonance energy transfer

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Chinese Chemical Letters 2023年第7期34卷 222-225页

作者： shu-min wang Hui wang Wei Zhao Jing-Juan Xu Hong-Yuan Chen State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China Institute of Nanochemistry and Nanobiology.School of Environmental and Chemical Engineering Shanghai UniversityShanghai 200444China

Plasmon resonance energy transfer(PRET) occurs between the plasmonic nanoparticles(NPs) and organic dyes forming donor-acceptor pairs, which has great potential in quantitative analytical chemistry because of its excellent sensitivity under dark-field microscopy(DFM). Herein, we introduce supramolecular β-cyclodextrin(β-CD) to design a host-guest recognition plasmonic nano-structure modified gold nanoparticles(GNPs), while GNPs and rhodamine molecule(RB) act as the donor and acceptor, respectively. In the presence of the target cholesterol, due to the stronger binding of cholesterol with β-CD, RB molecules are released, inducing the inhibition of PRET, as well as the increase of the scattering intensity of *** proposed strategy achieves a linear range from 0.02 μmol/L to 2.0 μmol/L for cholesterol detection,and reaches a limit of detection(LOD) of 6.7 nmol/L. This host-guest recognition strategy can easily integrate receptor-donor pair into one nanoparticle, which simplifies the construction of the PRET platform,and further provides an effective approach for PRET-based analytical applications. Afterwards, the proposed PRET strategy was successfully applied for the detection of cholesterol in serum samples with high sensitivity and specificity. The proposed method provides an effective clinically potential means for the detection of cholesterol and other disease-related biomarkers.

关键词： Dark field microscopy Localized surface plasmon resonance Plasmon resonance energy transfer GNPs@CD nanoparticles Cholesterol

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Successful management of infected right iliac pseudoaneurysm caused by penetration of migrated inferior vena cava filter:A case report

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World Journal of Clinical Cases 2021年第30期9卷 9211-9217页

作者： Cheng-Xin Weng shu-min wang Tie-Hao wang Ji-Chun Zhao Ding Yuan Department of Vascular Surgery West China Hospital of Sichuan UniversityChengdu 610041Sichuan ProvinceChina

BACKGROUND Indwelling inferior vena cava(IVC)filters might cause various complications,including filter penetration,filter fracture,filter migration,and thrombosis of the *** and migration complications are common,while a caudal migrated double-basket filter with associated infected iliac pseudoaneurysm has seldom been *** SUMMARY We report a 64-year-old female admitted for sudden onset of severe right abdominal pain after IVC filter placement for 3 *** patient had a history of failed endovascular IVC filter *** tomography showed that the retrieval hook of the filter penetrated the right common iliac artery and vein,leading to right iliac artery pseudoaneurysm accompanied by right ureteral obstruction with ipsilateral hydronephrosis,and bilateral iliac veins were *** open repair was performed to remove the IVC filter,the right iliac pseudoaneurysm,and the compromised segments of the iliac veins and IVC with right common iliac artery *** aureus was isolated from the tissue *** patient was discharged on postoperative day 12 with anticoagulation therapy and antibiotic therapy after ***-month follow-up computed tomography revealed that the right common iliac artery was patent,and only mild hydronephrosis was *** An indwelling IVC filter,even‘embedded’within organized thrombus,could still cause life-threatening *** procedures remain the last resort for IVC filters with severe complications.

关键词： Inferior vena cava filter Infected pseudoaneurysm Hydronephrosis Filter migration Open repair Antibiotic therapy Case report

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Oxidative stress and mitochondrial dysfunction of retinal ganglion cells injury exposures in long-term blue light

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International Journal of Ophthalmology(English edition) 2020年第12期13卷 1854-1863页

作者： Ke-Xin Guo Chen Huang Wei wang Pei Zhang Ying Li Zi-Yuan Liu min-shu wang Peking University Third Hospital Beijing 100191China Medical Research Center Peking University Third HospitalBeijing 100191China Department of Ophthalmology Peking University Third HospitalBeijing 100191China

AIM:To investigate the phototoxic effect of long-term excessive narrow-band blue light in staurosporine-induced differentiated retinal ganglion cells-5(SSRGC-5).METHODS:SSRGC-5 cells were divided into two groups,blue light group(BL group)and control *** viability was assessed by using CCK-8 *** profile analysis was performed by using Seahorse extracellular flux *** ultrastructure were studied via transmission electron microscope(TEM).Mitochondria contents and oxidative stress was evaluated by flow *** blotting was performed to monitor the changes in mitogen-activated protein kinases(MAPK)pathway and PI3 K/AKT ***:Blue light caused morphological changes of SSRGC-5 *** cell viability was significantly decreased from 3 h in BL *** ROS and mitochondrial superoxide levels were increased following blue light *** profiling identified blue light induced SSRGC-5 cells to have severely compromised mitochondrial *** was accompanied by impaired mitochondrial ultrastructure and remodeling,increased expression of the mitochondrial related proteins,and increased glycolysis as ***,the results showed that blue light induced higher expression of p-p38,p38,p-JNK,p-ERK,p-cJun,c-Jun,and ***:These findings indicate that excessive narrow-band blue light induces oxidative stress and mitochondrial metabolic remodeling dysregulate in SSRGC-5 *** MAPK and AKT signaling pathways are involved in this process.

关键词： blue light retinal ganglion cell oxidative stress mitochondria metabolism mitochondria abnormalities mitogen-activated protein kinases AKT

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Predictive value of a serum tumor biomarkers scoring system for clinical stage Ⅱ/Ⅲ rectal cancer with neoadjuvant chemoradiotherapy

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World Journal of Gastrointestinal Oncology 2022年第10期14卷 2014-2024页

作者： Jie-Yi Zhao Qing-Qing Tang Yu-Ting Luo shu-min wang Xiao-Rui Zhu Xiao-Yu wang Department of Neurosurgery West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina Department of Ophthalmology West China HospitalSichuan UniversityChengdu 610041Sichuan ProvinceChina West China Medical School Sichuan UniversityChengdu 610041Sichuan ProvinceChina

BACKGROUND Multiple classes of molecular biomarkers have been studied as potential predictors for rectal cancer(RC)*** antigen(CEA)is the most widely used blood-based marker of RC and has proven to be an effective predictive *** antigen 19-9(CA19-9)is another tumor biomarker used for RC diagnosis and postoperative monitoring,as well as monitoring of the therapeutic *** a panel of tumor markers for RC outcome prediction is a practical *** To assess the predictive effect of pre-neoadjuvant chemoradiotherapy(NCRT)CEA and CA19-9 levels on the prognosis of stage II/III RC *** CEA and CA19-9 levels were evaluated 1 wk before *** to the receiver operating characteristic curve analysis,the optimal cut-off point of CEA and CA19-9 levels for the prognosis were 3.55 and 19.01,*** novel serum tumor biomarker(NSTB)scores were as follows:score 0:Pre-NCRT CEA3.55 and CA19-9>19.01;score 1:Other *** information was recorded according to histopathological reports after the *** In the univariate analysis,pre-NCRT CEA<3.55[P=0.025 for overall survival(OS),P=0.019 for disease-free survival(DFS)],pre-NCRT CA19-9<19.01(P=0.014 for OS,P=0.009 for DFS),a lower NSTB score(0-1 vs 2,P=0.009 for OS,P=0.005 for DFS)could predict a better ***,in the multivariate analysis,only a lower NSTB score(0-1 vs 2;for OS,HR=0.485,95%CI:0.251-0.940,P=0.032;for DFS,HR=0.453,95%CI:0.234-0.877,P=0.019)and higher pathological grade,node and metastasis stage(0-I vs II-III;for OS,HR=0.363,95%CI:0.158-0.837,P=0.017;for DFS,HR=0.342,95%CI:0.149-0.786,P=0.012)were independent predictive *** The combination of post-NCRT CEA and CA19-9 was a predictive factor for clinical stage II/III RC patients receiving NCRT,and the combined index had a stronger predictive effect.

关键词： Rectal cancer Neoadjuvant chemoradiotherapy Scoring system Carcinoembryonic antigen Carbohydrate antigen 19-9 Predictive

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Effects of growth conditions on optical quality and surface morphology of InGaAsBi

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Chinese Physics B 2018年第4期27卷 509-513页

作者： Jia-Kai Li Li-Kun Ai ming Qi An-Hui Xu shu-min wang State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information Technology Chinese Academy of Sciences Shanghai 200050 China Department of Microtechnology and Nanoscience Chalmers University of Technology Gothenburg SE-41296 Sweden University of Chinese Academy of Sciences Beijing 100049 China

The effects of Bi flux and pressure of AsH3 on Bi incorporation,surface morphology and optical properties of InGa As Bi grown by gas source molecular beam epitaxy are *** is found that using relatively low pressure of AsH3 and high Bi flux can strengthen the effect on the incorporation of Bi and increase its content linearly with Bi flux until it nearly reaches a saturation *** result from Rutherford backscattering spectroscopy（RBS） confirms that the Bi incorporation can increase up to 1.13%.By adjusting Bi and As flux,we could improve the surface morphology of In Ga As Bi *** temperature photoluminescence shows strong and broad light emission at energy levels much smaller than the In Ga As bandgap.

关键词： compound semiconductor gas source molecular beam epitaxy bismide

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Production of human liver prolidase by Saccharomyces cerevisiae as host cells

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Acta Pharmacologica Sinica 2004年第6期25卷 92-98页

作者： shu-hao wang, min LIU, Mu-gen CHI, Qing-ding wang, Man-ji SUN2 Institute of Pharmacologyand Toxicology, Academy of Military Medical Science, Beijing 100850, China Institute of Pharmacology and Toxicology Academy of Military Medical Science 北京 100850

AIM: To clone and express the recombinant human liver prolidase in yeast and explore the activities of both dipeptidaseand organophosphoric acid anhydrolase(OPAA). METHODS:The cDNAencodinghuman liver prolidase derived from healthy adult liver was cloned into the pYES2, an expression vector of S cerevisiae, and then trans- formed into S cerevisiae INVSc1 by electroporation. The transformant with the highest enzymatic activity was induced by galactose for expression. The optimal induction conditions (temperature, induction time, and the initial amount of inoculation cells) were estimated by orthogonal experimental design. The recombinant prolidase and OPAA activities were assayed by spectrocolorimetric methods. RESULTS: The recombinant enzyme catalyzed the hydrolysis of organophosphorous compound soman as well as the hydrolysis of dipeptide Gly-Pro. Under the optimal induction conditions (20 h, 25 oC, initial OD600=0.4), the maximum activities of prolidase and OPAA came to 226.5 and 578 μmol·min-1·g-1 protein in cell lysate supernatants, respectively. SDS-PAGE of the recombinant enzyme in disrupted cell supernatants showed a molecular weight of 56 kDa. Intensity scanning of the SDS-PAGE gel revealed that the enzyme accounted for 3.16 % of the total protein in the supernatant. One liter incubation medium produced 7 g of wet yeast cell containing 4.56 mg of the recombination protein. CONCLUSION: The recombinant human liver prolidase produced by yeast cell (S cerevisiae) exhibited both dipeptidase and OPAA activities.

关键词： prolidase liver human S Cerevisiae organophosphorous compounds

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Glaucoma drainage device implantation and cyclophotocoagulation in the management of refractory glaucoma after Descemet-stripping automated endothelial keratoplasty

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International Journal of Ophthalmology(English edition) 2024年第2期17卷 257-264页

作者： min-shu wang Xue-Chuan Dong Mi-Yun Zheng Xiang Fan Ge-Ge Xiao Jing Hong Ling-Ling Wu Department of Ophthalmology Peking University Third HospitalBeijing 100191China Key Laboratory of Restoration of Damaged Ocular Nerve Beijing 100191China NIMO Ophthalmology Research Institute Beijing 100176China Department of Ophthalmology the First Hospital of PutianTeaching HospitalFujian Medical UniversityPutian 351100Fujian ProvinceChina

AIM:To compare the surgical outcomes of glaucoma drainage device implantation(GDI)and trans-scleral neodymium:YAG cyclophotocoagulation(CPC)in the management of refractory glaucoma after Descemetstripping automated endothelial keratoplasty(DSAEK).METHODS:This retrospective study on observational case series enrolled 29 patients who underwent DSAEK and posterior anti-glaucoma surgery(15 with GDI and 14 with CPC).The main outcome measures were intraocular pressure(IOP),glaucoma surgery success rate(defined as IOP of 6–21 mm Hg without additional anti-glaucoma operation),number of glaucoma medications,endothelial graft status,and best-corrected visual acuity(BCVA).RESULTS:The mean follow-up time was 34.1 and 21.0mo for DSAEK or glaucoma surgeries,both for the GDI and CPC *** groups showed significant IOP reduction after glaucoma *** GDI group presented a significantly higher success rate in IOP control than the CPC group(60%vs 21.4%,P=0.03).Both procedures significantly decreased the number of glaucoma medications(P=0.03).Forty percent and 57%of cases in the GDI and the CPC group,respectively,experienced endothelial graft failure during follow-up(P=0.36).Significantly worse BCVA after surgery was observed in the CPC group but not in the GDI ***:Both GDI and CPC significantly decrease IOP in eyes with glaucoma after *** is preferable to CPC in refractory glaucoma cases after DSAEK,as it manifests a significantly higher success rate for IOP control,similar endothelial graft failure rate,and relatively preserves BCVA than CPC.

关键词： glaucoma drainage device implantation cyclophotocoagulation refractory glaucoma Descemetstripping automated endothelial keratoplasty

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Cancer-cell-secreted CXCL11 promoted CD8+ T cells infiltration through docetaxel-induced-release of HMGB1 in NSCLC

Cancer-cell-secreted CXCL11 promoted CD8+ T cells infiltrati...

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第十三届全国免疫学学术大会

作者： Qun Gao shu min wang Yi Zhang The First Affiliated Hospital of Zhengzhou University

Background: Chemotherapy combined with immunotherapy becomes the main trend in lung cancer intervention;however, how chemotherapy promotes the immune function remains elusive. Therefore, we sought to determine how chemotherapy promotes the immune function. Methods: We determined in 77 NSCLC patients the expression of CD8, functional markers(IFN-γ, Granzyme B, and Perforin) and specific chemokines by quantitative real-time reverse transcriptase-PCR. Functional experiments were carried out to check whether docetaxel(DOC), a chemotherapeutic agent, modifies the expression of HMGB1 and CXCL11, and influences the infiltration properties of CD8 T cells to the tumor microenvironment. The mechanism of the release of HMGB1 and CXCL11 was determined by flow cytometry, immunofluorescence and western blotting. In in vivo experiment, we confirmed how DOC enhanced the recruitment of HER2-CAR T cells to tumor sites. Results: We found that DOC upregulated the expression of chemokine receptor ligand CXCL11 in tumor microenvironment and subsequently enhanced CD8 T cell recruitment. DOC treatment significantly increased HMGB1 release in an ROS-dependent manner. Recombinant protein HMGB1 stimulated the secretion of CXCL11 via NF-ΚB activation in vitro. Tumors from DOC-treated mice exhibited higher expression of HMGB1 and CXCL11, more HER2-CAR T cell infiltration, and reduced progression, relative to control. Increased HMGB1 and CXCL11 expressions were positively correlated with prolonged overall survival of lung cancer patients. Conclusions: Our results demonstrate that DOC induces CD8 T cell recruitment to the tumor microenvironment by enhancing the secretion of HMGB1 and CXCL11, thus improving the anti-tumor efficacy, indicating that modulating the HMGB1-CXCL11 axis might be helpful for NSCLC treatment.

关键词： Docetaxel CXCL11 CD8+ T cells HER2-CAR T cells high-mobility group box-1

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Acta Pharmacologica Sinica 2003年第12期24卷 91-95页

作者： LIU Hui-Chen, JIN shu-min, wang Yong-Li Department of Pharmacology, Hebei Medical University, Shijiazhuang 050017, China Department of Pharmacology Hebei Medical University Shijiazhuang 050017

AIM: To compare the pharmacokinetics of the enantiomers of trans-tramadol (trans-T) and its active metabolite, trans-O-demethyltramadol (M1), in male and female rats. METHODS: Following a single oral dose of 10 mg/kg trans-T hydrochloride to rats, (+)-trans-T, (-)-trans-T, (+)-M1, and (-)-M1 in plasma were determined by a high performance capillary electrophoresis method. RESULTS: The females showed higher plasma concentrations of (+)-trans-T, (-)-trans-T, and (+)-M1 than the males. The enantiomers of trans-T were absorbed and eliminated more slowly in the females than in the males. (+)-M1 was eliminated more slowly in the females than in the males. All pharmacokinetic parameters but Tmaxof the two enantiomers of trans-T were significantly different in both sex rats. The (+)/(-)-enantiomeric ratios of the pharmacokinetic parameters for trans-T in the males were similar to those in the females. The values of Cmax, AUC0-∞of the two enantiomers of M1 were significantly different in both sex rat

关键词： tramadol pharmacokinetics sex factors

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Surgical complexity and prognostic outcome of small volume renal cell carcinoma with high-level venous tumor thrombus and large volume renal cell carcinoma with low-level thrombus

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Chinese Medical Journal 2019年第15期132卷 1780-1787页

作者： Zhuo Liu Xun Zhao Hong-Xian Zhang Li-Wei Li Shi-Ying Tang Guo-Liang wang shu-Dong Zhang shu-min wang Lu-Lin Ma Xiao-Jun Tian Department of Urology Peking University Third Hospital Beijing 100191 China Department of Ultrasound Peking University Third Hospital Beijing 100191 China

Background:Radical nephrectomy with thrombectomy is one of the most difficult and complicated urological *** the roles of renal tumor volume and thrombus level in surgical complexity and prognostic outcome are not *** study aimed to evaluate the surgical complexity and prognostic outcome between the volume of renal cell carcinoma (RCC) and the level of venous tumor ***:The clinical data of 67 RCC cases with renal vein or inferior vena cava (IVC) tumor thrombus from January 2015 to May 2018 were retrospectively *** these 67 cases,21 (31.3%) were small tumors with high-level thrombus (tumor ≤7 cm in diameter and thrombus Neves Level Ⅱ-Ⅳ),while 46 (68.7%) were large tumors with low-level thrombus group (tumor >7 cm in diameter and thrombus Level 0-Ⅰ).Clinical features,operation details,and pathology data were *** and multivariable logistic regression analyses were applied to evaluate the risk factors for small tumor with high-level ***:Patients with small tumors and high-level thrombus were more likely to have longer operative time (421.9 ± 135.1 min vs.282.2 ± 101.9 min,t=4.685,P < 0.001),more surgical bleeding volume (1200 [325,2900] mL vs.500 [180,1000] mL,U =270.000,P =0.004),more surgical blood transfusion volume (800 [0,1400] mL vs.0 [0,800] mL,U =287.500,P =0.004),more plasma transfusion volume (0 [0,800] mL vs.0 [0,0] mL,U =319.000,P =0.004),higher percentage of open operative approach (76.2% vs.32.6%,x2 =11.015,P =0.001),higher percentage of IVC resection (33.3% vs.0%,x2 =17.122,P < 0.001),and higher percentage of post-operative complications (52.4% vs.19.6%,x2 =7.415,P =0.010) than patients with large tumors and low-level *** multivariate analysis,decreased hemoglobin (Hb)(odds ratio [OR]:0.956,95 % confidence interval [CI]:0.926-0.986,P =0.005) and non-sarcomatoid differentiation (OR:0.050,95% CI:0.004-0.664,P =0.023) were more likely to form small tumors with high-level tumor t

关键词： Inferior vena cava Tumor thrombus Renal cell carcinoma Cancer-specific survival Prognosis Neves classification

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