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Increased excitatory amino acid transporter 2 levels in basolateral amygdala astrocytes mediate chronic stress–induced anxiety-like behavior

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Neural Regeneration Research 2025年第6期20卷 1721-1734页

作者： Xirong Xu Shoumin Xuan shuai Chen Dan Liu Qian xiao Jie Tu Shenzhen Key Laboratory of Neuroimmunomodulation for Neurological Diseases Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina CAS Key Laboratory of Brain Connectome and Manipulation Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Brain Connectome and Behavior the Brain Cognition and Brain Disease Institute(BCBDI)Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina University of Chinese of Academy of Sciences BeijingChina Faculty of Life and Health Sciences Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong ProvinceChina

The conventional perception of astrocytes as mere supportive cells within the brain has recently been called into question by empirical evidence, which has revealed their active involvement in regulating brain function and encoding behaviors associated with ***, astrocytes in the basolateral amygdala have been found to play a role in the modulation of anxiety-like behaviors triggered by chronic stress. Nevertheless, the precise molecular mechanisms by which basolateral amygdala astrocytes regulate chronic stress–induced anxiety-like behaviors remain to be fully elucidated. In this study, we found that in a mouse model of anxiety triggered by unpredictable chronic mild stress, the expression of excitatory amino acid transporter 2 was upregulated in the basolateral amygdala. Interestingly, our findings indicate that the targeted knockdown of excitatory amino acid transporter 2 specifically within the basolateral amygdala astrocytes was able to rescue the anxiety-like behavior in mice subjected to stress. Furthermore, we found that the overexpression of excitatory amino acid transporter 2 in the basolateral amygdala, whether achieved through intracranial administration of excitatory amino acid transporter 2agonists or through injection of excitatory amino acid transporter 2-overexpressing viruses with GfaABC1D promoters, evoked anxiety-like behavior in mice. Our single-nucleus RNA sequencing analysis further confirmed that chronic stress induced an upregulation of excitatory amino acid transporter 2 specifically in astrocytes in the basolateral amygdala. Moreover, through in vivo calcium signal recordings, we found that the frequency of calcium activity in the basolateral amygdala of mice subjected to chronic stress was higher compared with normal *** knocking down the expression of excitatory amino acid transporter 2 in the basolateral amygdala, the frequency of calcium activity was not significantly increased, and anxiety-like behavior was ob

关键词： anxiety astrocytes basolateral amygdala behavior dihydrokainic acid excitatory amino acid transporter 2 fiber photometry glutamate LDN-212320 transporter

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Influence of process parameters on interracial tensile strength of crosswedge rolling of 42CrMo/Q235 laminated shafts

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Journal of Iron and Steel Research International 2018年第10期25卷 1003-1009页

作者： Wen-fei Peng Chao Yan xiao Zhang Xue-dao shu Guang-xing Huang dong-ming Xu Zhejiang Provincial Key Lab of Part Rolling Technology Ningbo University Ningbo 315211 Zhejiang China Faculty of Mechanical Engineering and Mechanics NingboUniversity Ningbo 315211 Zhejiang China

Through rolling experiments and interfacial tensile strength tests of cross-wedge rolled laminated shafts of 42CrMo/Q235 composites, the influence of process parameters, including forming angle, spreading angle, area reduction, rolling temperature and core material diameter on the interfacial shear strength was analyzed. The results show that the sequence of process parameters in order of greatest influence on interfacial tensile strength was rolling temperature, area reduction, core material diameter, forming angle and spreading angle. At the interface of the combined materials, tensile strength decreased as forming angle and spreading angle increased, whereas the tensile strength first increased and then decreased as area reduction, rolling temperature and core material diameter increased.

关键词： Laminated shaft Process parameter Interfacial tensile strength Cross-wedge rolling

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Coupled interactive effects of ammonia and hydrogen additions on ethylene diffusion flames: A detailed kinetic study

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Science China(Technological Sciences) 2024年第3期67卷 930-948页

作者： xiao JiaYi XU TingTing shu ZhiMei DENG QiGe ZHAO Xuan YING YaoYao LI TianJiao LIU dong MIIT Key Laboratory of Thermal Control of Electronic Equipment School of Energy and Power EngineeringNanjing University of Science and TechnologyNanjing 210094China Advanced Combustion Laboratory School of Energy and Power EngineeringNanjing University of Science and TechnologyNanjing210094China

In order to create effective combustion technologies and fuels with low or no carbon emissions,the research was conducted to assess the coupled interactive effects of NH_(3) and H_(2) additions on ethylene counterflow diffusion flames from a kinetic *** effects of the NH_(3)/H_(2) combination on flame temperatures,major species,key radicals,important intermediate species,representative oxygenated species and NO_xwere *** results of the study utilizing fictitious inert NH_(3) and/or H_(2) revealed the chemical effects of the two *** was found that the NH_(3)/H_(2) coupled effects had a more effective inhibitory effect on soot precursors than the effects of corresponding sum of single NH_(3) or H_(2) *** production of soot precursors was promoted by the coupled chemical effects of NH_(3) and H_(2),but the coupled dilution and thermal effects were observed to have a greater impact,resulting in a decrease of the mole fractions of soot *** for the interaction of NH_(3) and H_(2) effects,the presence of H_(2) decreased the chemical effects of NH_(3) on the augmentation of C_(2)H_(2),A1,A2,and CH_(3)CHO mole *** NH_(3) addition alleviated the H_(2) chemical effects on increasing C_(2)H_(2),C_(3)H_(3),A1 and A2 ***,the NH_(3)chemical effects on C_(3)H_(3),OH and CH_(3)CHO were enhanced when H_(2) was *** presence of NH_(3) augmented the chemical effects of H_(2) on the growth of OH mole ***,the H_(2) chemical effects hindered the production of NO and NO_(2) in the presence of NH_(3).

关键词： NH_(3)/H_(2)addition kinetic analysis coupled interactive effects chemical effects

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Acupoint Autohemotherapy Attenuates Atopic Dermatitis Lesions by Regulating Th1/Th2 Balance in DNCB-Induced BALB/c Mice

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Chinese Journal of Integrative Medicine 2022年第7期28卷 612-619页

作者： ZENG Zhi-wen HUANG Jin-quan CHEN Yong YU xiao ZHU Wei ZHANG dong-shu School of Traditional Chinese Medicine Southern Medical UniversityGuangzhou 510515China Department of Traditional Chinese Medicine Affiliated Dongguan People's HospitalSouthern Medical UniversityDongguanGuangdong Province 523058China Department of Rheumatology and Immunology Shenzhen People's HospitalThe Second Clinical Medical College of Jinan UniversityShenzhenGuangdong Province 518020China School of Basic Medical Sciences Southern Medical UniversityGuangzhou 510515China Guangzhou Center for Disease Control and Prevention Guangzhou 510515China

Objective: To evaluate the therapeutic effects of acupoint autohemotherapy(A-AHT) on 1-chloro-2,4-dinitrobenzene(DNCB)-induced atopic dermatitis(AD) in mice focusing on regulating T helper 1/T helper 2(Th1/Th2) immune responses. Methods: Thirty BALB/c mice were divided into 5 groups by a random number table, including normal control(NC), AD model(AD), A-AHT, sham A-AHT(sA-AHT), and acupoint injection of normal saline(A-NS) groups, 6 mice per group. Mice were challenged by DNCB for the establishment of experimental AD model. On the 8th day, except for the NC and AD groups, the mice in the other groups received management once every other day for a total of 28 days. For the A-AHT and sA-AHT groups, 0.05 mL of autologous whole blood(AWB) was injected into bilateral Zusanli(ST 36) and Quchi(LI 11) and sham-acupoints(5 mm lateral to ST 36 and LI 11), respectively. The A-NS group was administrated with 0.05 m L of normal saline by acupoint injection into ST 36 and LI 11. Dermatitis severity for dorsal skin of mice was determined using the Severity Scoring of Atopic Dermatitis(SCORAD) every week. The total immunoglobulin E(IgE), interleukin-4(IL-4), and interferon-gamma(IFN-γ) cytokine levels in serum were examined by enzyme-linked immunosorbent assay(ELISA). Spleen Th1/Th2 expression were analyzed via flow cytometry and immunohistochemical assay was used to detect T-box expressed in T cell(T-bet) and GATA-binding protein 3(GATA3) expressions in skin lesions of mice. Results: Compared with the AD group, both A-AHT and sA-AHT reduced the SCORAD index and serum IgE level(P<0.05 or P<0.01);A-AHT, sA-AHT and A-NS down-regulated serum IL-4 level and upregulated IFN-γ/IL-4 ratio(P<0.05 or P<0.01);A-AHT regulated the Th1/Th2 shift specifically and increased the related transcription factors such as T-bet expression and T-bet/GATA3 ratio(P<0.05). Conclusion: A-AHT showed significant effectiveness on the AD model mice, through regulating Th1/Th2 immune responses.

关键词： acupoint autohemotherapy atopic dermatitis Th1/Th2 immunoglobulin E

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Modeling the carbon dynamics of alpine grassland in the Qinghai-TibetanPlateau under scenarios of 1.5 and 2 ℃ global warming

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Advances in Climate Change Research 2019年第2期10卷 80-91页

作者： YI shu-Hua XIANG Bo MENG Bao-Ping WU xiao-dong DING Yong-Jian Instiute o f Fragile Ecosystem and Environment Nantong University Nantong 226007 China of Geographic Sciences Nantong University Nantong 226007 China State Key Laboratory of Cryospheric Science Northwest Institute of Eco-Environment and Resources Chinese Academy of Sciences Lanzhou 730000 China Chongqing Climate Center Chongqing 401147 China Cryosphere Research Station on the Qinghai-Tibetan Plateau Lanzhou 730000 China Key Laboratory of Ecohydrology of Inland River Basin Chinese Academy of Sciences Lanzhou 730000 China University of Chinese Academy Sciences Beijing 100049 China

Alpine grassland occupies two-thirds of the Qinghai-Tibetan Plateau (QTP). It is vital to project changes of this vulnerable ecosystem under different climate change scenarios before taking any mitigation or adaptation measures. In this study, we used a process-based ecosystem model, driven with output from global circulation models under different Representative Concentration Pathways (RCPs), to project the carbon dynamics of alpine grassland. The results showed the following: 1) Vegetation carbon (C) on the QTP increased by 22—38 gC m^-2 during periods of 1.5 and 2 ℃ warming under different RCPs when compared to the baseline period (1981—2006), while soil C increased by 85—122 gC m^-2. 2) The increases of vegetation C and soil C at the period of 1.5 ℃ warming were about 15 gC m^-2 and 40 gC m^-2 smaller than those at the period of 2 ℃ warming, respectively;increase of C was greater for alpine meadow than for alpine steppe. 3) Precipitation, radiation, and permafrost changed significantly and showed heterogeneous spatial patterns, and caused heterogeneous response of C dynamics. For alpine meadow in regions transformed from permafrost to seasonally frozen soil with medium annual precipitation (200—400 mm), vegetation C and net primary production decreased by 18.7 gC m-2 and 3.1 gC m^-2 per year during 2 °C warming under RCP 4.5, respectively. This decrease can be attributed to the disappearing impermeable permafrost. Different from previous studies that indicated an unfavorable response of alpine grassland to climate warming, this study showed a relatively favorable response, which is mainly attributed to C 0 2 fertilization.

关键词： Permafrost Terrestrial ecosystem model Vegetation Soil Net primary production

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Blue and Violet Cerenkov Second Harmonic Generation of Laser Diodes in Proton Exchanged MgO:LiNbO_(3) Optical Waveguide

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Chinese Physics Letters 1996年第10期13卷 741-743页

作者： MU xiao-dong SHAO Zong-shu ZHANG Heng-li KONG Qing-yu JIANG Min-hua National Laboratory of Crystal Materials Shandong UniversityJinan 250100

Using the Cerenkov second harmonic generation, 478 nm blue and 402nm violet light are generated by direct frequency doubling of 956 and 804 nm diode lasers in a same proton exchanged MgO:LiNbO3 waveguide. Under the low fundamental guide wave power of less than 1mW, the estimated value of the output blue and violet light power is in the order of 0.1-0.01μW.

关键词： Waveguide violet doubling

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Identification and functional analysis of arabinogalactan protein expressed in pear pollen tubes

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Journal of Integrative Agriculture 2023年第3期22卷 776-789页

作者： JIAO Hui-jun WANG Hong-wei RAN Kun dong xiao-chang dong Ran WEI shu-wei WANG Shao-min Shandong Institute of Pomology Tai’an 271000P.R.China

Arabinogalactan proteins(AGPs)are widely distributed in the plant kingdom and play a vital role during the process of plant sexual *** this study,we performed a comprehensive identification of the PbrAGPs expressed in pear pollen and further explored their influences on pollen tube *** the 187 PbrAGPs that were found to be expressed in pear pollen tubes,38 PbrAGPs were specifically expressed in pollen according to the RNA-seq *** PbrAGPs were divided into two groups of highly expressed and specifically expressed in pear *** further tested their expression patterns using RT-PCR and *** of the PbrAGPs were expressed in multiple tissues and their expression levels were consistent with reads per kilobase per million map reads(RPKM)values during pollen tube growth,implying that PbrAGPs might be involved in the regulation of pear pollen tube *** also constructed phylogenetic trees to identify the functional genes in pear pollen tube ***,19 PbrAGPs(PbrAGP1 to PbrAGP19)were selected to test their influences on pollen tube *** proteins of the 19 PbrAGP-His were purified and used to treat pear pollen,and 11 of the PbrAGP-His recombinant proteins could promote pear pollen tube ***,pollen tube growth was inhibited when the expression levels of PbrAGP1 and PbrAGP5 were knocked down using an antisense oligonucleotide ***1 and PbrAGP5 were localized in the plasma membrane and might not alter the distribution of pectin in the pollen *** summary,this study identified the PbrAGPs expressed in pear pollen and lays the foundation for further exploring their functions in pollen tube growth.

关键词： arabinogalactan proteins pear pollen tube antisense oligodeoxynucleotides sexual reproduction

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PON1、HtrA4与拉贝洛尔治疗子痫前期合并代谢综合征效果的相关性研究

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河北医科大学学报 2020年第9期41卷 1053-1057页

作者：董淑晓刘志虎刘慧丽石国素郑莉霞河北省邢台市第三医院妇产科河北邢台054000 河北省邢台市人民医院妇产科河北邢台054000

目的探讨氧磷酯酶1(paraoxonase-1,PON1)、丝氨酸肽酶4(HtrA serine peptidase 4,HtrA4)与拉贝洛尔治疗子痫前期合并代谢综合征效果的相关性。方法选择采用拉贝洛尔治疗的子痫前期患者114例,分为子痫前期不合并代谢综合征组(n=42)和子... 详细信息

目的探讨氧磷酯酶1(paraoxonase-1,PON1)、丝氨酸肽酶4(HtrA serine peptidase 4,HtrA4)与拉贝洛尔治疗子痫前期合并代谢综合征效果的相关性。方法选择采用拉贝洛尔治疗的子痫前期患者114例,分为子痫前期不合并代谢综合征组(n=42)和子痫前期合并代谢综合征组(n=72),另选取正常妊娠妇女作为正常妊娠组(n=50)。比较3组治疗前后血清PON1、HtrA4水平。根据治疗效果将子痫前期合并代谢综合征组患者分为显效组、有效组和无效组,比较其治疗前后血清PON1、HtrA4水平,并进行相关性检验。结果治疗前,子痫前期合并代谢综合征组血清PON1水平低于子痫前期不合并代谢综合征组和正常妊娠组,血清HtrA4水平高于子痫前期不合并代谢综合征组和正常妊娠组(P<0.05)。子痫前期不合并代谢综合征组血清PON1水平低于正常妊娠组,血清HtrA4水平高于正常妊娠组(P<0.05)。治疗后,子痫前期合并代谢综合征及子痫前期不合并代谢综合征组血清PON1水平高于治疗前,血清HtrA4水平低于治疗前(P<0.05)。子痫前期合并代谢综合征组血清PON1水平低于子痫前期不合并代谢综合征组,血清HtrA4水平高于子痫前期不合并代谢综合征组(P<0.05)。治疗后,子痫前期合并代谢综合征组显效28例,有效36例,无效8例。治疗后,显效组、有效组、无效组血清PON1水平高于治疗前,血清HtrA4水平低于治疗前,显效组血清PON1水平高于有效组和无效组,血清HtrA4水平低于有效组和无效组,有效组血清PON1水平高于无效组,血清HtrA4水平低于无效组(P<0.05)。血清PON1水平与临床疗效呈正相关(P<0.05),血清HtrA4水平与临床疗效呈负相关(P<0.05)。结论血清PON1、HtrA4可能参与子痫前期合并代谢综合征的发生,与采用拉贝洛尔治疗的临床效果具有相关性,可能成为评估子痫前期合并代谢综合征治疗效果的新的生物学指标。

关键词：子痫代谢综合征对氧磷酯酶1 丝氨酸肽酶4

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Melatonin treatment alleviates chilling injury in mango fruit 'Keitt' by modulating proline metabolism under chilling stress

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Journal of Integrative Agriculture 2023年第3期22卷 935-944页

作者： Mariama KEBBEH dong Jing-xian HUAN Chen SHEN shu-ling LIU Yan ZHENG xiao-lin College of Food Science and Biotechnology Zhejiang Gongshang UniversityHangzhou 310018P.R.China

Mangoes often suffer from low temperature-induced chilling injury (CI) during postharvest cold ***,advanced techniques are crucial and in high demand to solve the chilling stress of mango fruit for a higher *** study addresses chilling stress modulation by investigating the effects of melatonin treatment on CI,proline metabolism,and related gene expressions of ‘Keitt’ mango during cold storage after dipped in 0 (control),0.1 (MT1),and 0.2 mmol L^(-1)(MT2) melatonin solution for 30 *** results revealed that melatonin treatment in MT1 significantly reduced CI development and increased proline content in mango fruit during cold storage compared to the *** changes were along with increases in the activity of critical enzymes as well as the expression of encoding genes involved in proline biosynthesis,such as pyrroline-5-carboxylate synthetase (P5CS),pyrroline-5-carboxylate reductase (P5CR),ornithine D-aminotransferase (OAT),P5CS2,P5CR2,and ***,proline dehydrogenase (PDH) activity and the expression of the PDH3 gene associated with proline dehydrogenation were lower in MT1-treated mangoes than the controlled ***,melatonin treatment has regulated proline metabolism resulting in the accumulation of proline,subsequently contributing to enhancing the chilling tolerance of‘Keitt’mango fruit.

关键词： chilling injury(CI) cold storage mango fruit melatonin proline metabolism

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Comparative Proteomic Analysis Shows an Elevation of Mdh1 Associated with Hepatotoxicity Induced by Copper Nanoparticle in Rats

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Journal of Integrative Agriculture 2014年第5期13卷 1073-1081页

作者： dong shu-wei GAO Zhao-hui SHEN xiao-yun XUE Hui-wen LI Xia Key Laboratory of Veterinary Pharmaceutical Development Ministry of Agriculture/Key Laboratory of New Animal Drug ProjectGansu Province/Lanzhou Institute of Husbandry and Pharmaceutical Sciences Chinese Academy of Agricultural Sciences College of Veterinary Medicine Gansu Agricultural University Bijie College

Copper nanoparticle is a new material widely used in biological medicine, animal husbandry and industrial areas, but its potential toxicity to human health and environment remains unclear. In order to study the hepatotoxic mechanisms of nanoparticles copper, two-dimensional gel electrophoresis （2-DE） and matrix-assisted laser desorption/ionization time of flight mass spectrometry （MALDI/TOF MS） of proteomics technology were used to isolate and identify the differentially expressed proteins from liver, which associated with hepatotoxicity induced by copper nanoparticle in rats. In this study, we have screened 15 kinds of proteins related with hepatotoxicity, of which spot8212 was identified as Malate dehydrogenase （Mdhl）. The mRNA expression trend of Mdhl was consistent with the result of 2-DE by RT-PCR validation. Bioinformatics analysis showed that Mdhl was stable and no signal peptides, subcellular location was in endoplasmic reticulum; it contained many functional sites such as malate dehydrogenase activity signal sites 155LTRLDHNRAKSQI167; a helixes and random coils were the two main elements. Homologous analysis demonstrated high homologous of Mdhl in rats with mouse and human, and the phylogenetic tree of Mdhl was constructed. The result indicated that copper nanoparticle could regulate up the Mdhl protein expression so as to compensate the energy deficit. Energy metabolic disturbance may be a pathway for copper nanoparticle particles to exert the hepatotoxic effects in rats.

关键词： copper nanoparticle hepatotoxicity proteomics malate dehydrogenase bioinformatics rat

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