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World Journal of Gastroenterology 2007年第31期13卷 4255-4259页

作者： Zhi-Hua Ran Qi xu Jin-Lu Tong shu-dong xiao Departmentof Gastroenterology Renji Hospital Shanghai Jiao TongUniversity School of Medicine Shanghai Institute of DigestiveDisease Shanghai 200001 China

To investigate whether Epigallocatechin-3-gallate （EGCG） can induce apoptosis of the gastric cancer cell line MKN45 and its apoptotic ***： To determine this, apoptotic rates of MKN45 cells after EGCG treatment with or without caspase-3 inhibitor were evaluated by Annexin V-FITC ＋ PI staining The influence of EGCG on the activity of caspase-3 in the MKN45 cells was determined by ELISA. By Rhodamine123 staining, the membrane potential change of the mitochondrion was also investigated, and mRNAs and protein expression of the bcl-2 family were analyzed by RT-PCR and Western ***： EGCG can induce apoptosis of MKN45 cells in time- and dose-dependent manner. Eight hours after EGCG treatment, the activity of caspase-3 in the MKN45 increased, especially 12 h after treatment. The mitochondrial membrane potential was significantly weakened 4 h after EGCG insult. The mRNA and protein expression levels of pro-apoptotic members, such as Bax, Bid and Bad, were upregulated gradually as treated time increased. Moreover, the mRNA and protein expression levels of anti-apoptotic members, such as Bcl-xL and Bcl-2, were inhibited. CONCLUSION： These data support that EGCG can induce apoptosis of the human gastric cancer cell line MKN45, and the effect is in a time- and dose-dependent manner. The apoptotic pathway triggered by EGCG in MKN45 is mitochondrial-dependent.

关键词： Epigallocatechin-3-gallate Stomachneoplasms Bcl-2 Apoptosis

A subset of ulcerative colitis with positive proteinase-3 antineutrophil cytoplasmic antibody

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World Journal of Gastroenterology 2008年第45期14卷 7012-7015页

作者： Jin Xu Chuan-Hua Yang xiao-Yu Chen Xu-Hang ki Min Dai shu-dong xiao Department of Internal Medicine Division of Gastroenterology Shanghai Renji Hospital Shanghai Institute of Digestive Disease Shanghai Jiaotong University School of Medicine Shanghai 200001 China Department of Medicine GI Division Johns Hopkins University School of Medicine Baltimore MD 21205 United States Department of Internal Medicine Division of Rheumatology Shanghai Renji Hospital Shanghai Jiaotong University School of Medicine Shanghai 200001 China

A small subset of patients with active ulcerative colitis is non-responsive to major known non-biological therapies. We reported 5 patients with positive serum proteinase-3 antineutrophil cytoplasmic antibody (PR3-ANCA) and tried to (1) identify the common clinical features of these patients; (2) investigate the efficacy of a novel therapy using a Chinese medicine compound; and (3) attract more gastroenterologists to be engaged in further study of this subset of patients. The common manifestations of disease in these 5 patients included recurrent bloody diarrhea and inflammatory lesions involving the entire colorectal mucosa. Initial treatment with intravenous methylprednisolone successfully induced remission. Four of these 5 patients were steroid-dependence, and immunosuppressants, such as azathioprine and cyclophosphamide, were in effective. In 3 patients, only the particular Chinese medicine compound could induce and maintain remission. One patient underwent colectomy. No vascular inflammatory lesions were found by histopathological examination. Although more cases are needed for confirmation, our study indicates thatulcerative colitis with positive PR3-ANCA may belong to a subtype of refractory ulcerative colitis. The particular Chinese medicine compound used in our study is by far the most effective in the management of these patients, with additional advantages of having no noticeable side-effects and less financial burden.

关键词： Refractory ulcerative colitis Proteinase-3 antineutrophil cytoplasmic antibody Methyprednisolone Steroid-dependence Chinese medicine

Resistance of Helicobacter pylori to antibiotics from 2000 to 2009 in Shanghai

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World Journal of Gastroenterology 2010年第40期16卷 5118-5121页

作者： Qin-Juan Sun xiao Liang Qing Zheng Wei-Qi Gu WenZhong Liu shu-dong xiao Hong Lu Department of Gastroenterology Shanghai Renji HospitalShanghai Institute of Digestive DiseaseShanghai Jiaotong University School of MedicineShanghai 200001China

AIM: To investigate the resistance of Helicobacter pylori (H. pylori ) to 6 commonly used antibiotics from 2000 to 2009 in Shanghai. METHODS: A total of 293 H. pylori strains were collected from 2000 to 2009 in Shanghai and tested for their susceptibility to metronidazole, clarithromycin, amoxicillin, furazolidone, levofloxacin and tetracycline using agar dilution. RESULTS: The resistant rates of H. pylori to clarithromycin (8.6%, 9.0% and 20.7%) and levofloxacin (10.3%, 24.0% and 32.5%) increased from 2000 to 2009 in Shanghai. The resistant rate of H. pylori to metronidazole remained stable (40%-50%). Only one strain of H. pylori isolated in 2005 was resistant to tetracycline. All strains were sensitive to amoxicillin and *** resistant rate of H. pylori to antibiotics was not related with the sex, age and clinical outcome of patients. CONCLUSION: Resistance of H. pylori to antibiotics plays an important role in making treatment strategies against H. pylori -associated diseases.

关键词： Helicobacter pylori Antibiotic resistance Agar dilution Metronidazole Clarithromycin Levofloxacin Tetracycline Amoxicillin Furazolidone

Predictive value of neutrophil infiltration as a marker of Helicobacter pylori infection

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World Journal of Gastroenterology 2012年第36期18卷 5101-5105页

作者： xiao-Qing Xu Zhen-Hua Wang Jing-Xian Liao xiao-Yu Chen Wen-Zhong Liu shu-dong xiao Hong Lu Depart-ment of Gastroenterology Shanghai Renji HospitalShanghai Institute of Digestive DiseaseShanghai Jiaotong University School of Medicine145 Middle Shandong RdShanghai 200001China

AIM： To evaluate the predictive value of neutrophil in- filtration as a marker of Helicobacter pylori （H. pyloni） infection. METHODS： A total of 315 patients with dyspepsia symptoms who underwent upper gastrointestinal en- doscopy were enrolled in this study. Biopsies were evaluated using the updated Sydney system. The medication history of all patients in the preceding 4 wk was recorded. The diagnosis of H. pylori infection was based on 13C-urea breath test at least 4 wk after with- drawal of antisecretory drugs, antibiotics and related drugs. For the patients with subtotal gastrectomy, the diagnosis of H. pylori infection was based on anti-H. pylori immunoglobulin G （IgG） antibody. Serum anti-H. pylori IgG antibody was measured by enzyme-linked immunosorbent assays （Biohit, Finland）. RESULTS： The sensitivity, specificity, positive predic- tive value and negative predictive value of neutrophil infiltration in the diagnosis ofH, pylorlinfection were 92.3%, 83.5%, 77.4% and 94.7%, respectively. Neu- trophil infiltration of gastric mucosa in the histological analysis was strongly associated withH, pylorlinfection （77.4% vs 5.3% in the neutrophil infiltration negative group, P = 0.000）. Moderate neutrophil infiltration was more frequent in H. pylorl infection when compared to mild infiltration （81.8% and 75%, respectively）, but did not reach statistical significance. For those patients with negative rapid urease test, H. pylori was detected in 73.2% of patients with positive neutrophil infiltration on histology. In patients with subtotal gastrectomy, the diagnostic accuracy of neutrophil infiltration in H. pylori infection was 50%. CONCLUSION： Neutrophil infiltration is closely associ- ated withH, pylori and may be recognized as a sign of this infection.

关键词： Helicobacter pylori Histology Neutrophil Sensitivity Specificity

Eradication of H pylori infection in a rural population: One-day quadruple therapy versus 7-day triple therapy

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World Journal of Gastroenterology 2006年第24期12卷 3915-3918页

作者： Lian Zhang Lin Shen Jun-Ling Ma Kai-Feng Pan Wei-dong Liu Jie Li shu-dong xiao San-Ren Lin Meinhard Classen Wei-Cheng You Department of Cancer Epidemiology Peking University School of Oncology Beijing Cancer Hospital and Institute Beijing 100036 China Department of Internal Medicine PekingUniversity School of Oncology Beijing Cancer Hospital and Institute Beijing 100036 China Linqu Public Health Bureau Linqu Shandong 262600 China Shanghai Institute for Digestive Disease Shanghai China The Third Hospital of Peking University Beijing China Technical University Munich Germany

AIM： To compare the one-day quadruple therapy with a standard 7-d triple therapy for H pylori eradication in a rural population of China. METHODS： A total of 396 patients with 13C-urea breath test positive for H pylori were assigned into two groups： 239 patients received one-day quadruple therapy （amoxicillin 2000 mg qid; metronidazole 500 mg qid; bismuth citrate 900 mg qid and lansoprazole 60 mg once daily） and 157 patients received 7-d standard triple therapy （amoxicillin 1000 mg bid; clarithromycin 500 mg bid and lansoprazole 30 mg bid）. All the patients underwent a 13C-UBT to assess the eradication of Hpylori infection six weeks after treatment. RESULTS： Two hundred and twenty-nine patients completed the one-day therapy （95.8%） and 148 patients completed the 7-d therapy （94.2%）. The oneday therapy eradicated H pylori infection in 64 patients （27.95%）. In contrast, 103 patients （69.59%） were Hpylori negative after the 7-d therapy （P 〈 0.01）. CONCLUSION： This pilot study suggests there is no beneficial effect of the one-day therapy in treatment ofHpylori infection compared with the 7-d standard therapy.

关键词： Eradication H pylori infection Quadrupletherapy Triple therapy

Multi-wavelength fiber laser based on broadband fiber grating and HiBi fiber loop mirror

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Optoelectronics Letters 2007年第1期3卷 30-33页

作者： JIN Yan-li LIU Yan-ge LIU Jian-guo LI Yan YUAN shu-zhong dong xiao-yi Institute of Modern Optics Nankai University Tianjin 300071 China

A fiber laser based on broadband C-band fiber grating (CFG) and high birefringence (HiBi) fiber loop mirror (FLM) is demonstrated, which uses the comb-like reflection performance of the HiBi FLM. Under different polarization states, different output lasers are gained by choosing the polarization states of the light in the cavity using the polarization controller. By this method, a single wavelength or dual wavelength laser beam can be output, and multi-wavelength oscillates can be achieved.

关键词：多波长光纤激光器宽带光纤光栅 HiBi光纤环镜极化控制器

Correlation between coagulation function, tumor stage and metastasis in patients with renal cell carcinoma： a retrospective study

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Chinese Medical Journal 2011年第8期124卷 1205-1208页

作者： xiao Bo MA Lu-lin ZHANG shu-dong xiao Chun-lei LU Jian HONG Kai LIAO Hong-yi Department of Urology Peking University Third Hospital Beijing100191 China

Background The coagulation function in carcinoma patients is abnormal, but in renal cell carcinoma the extent and relationships of coagulation function remain unclear. This study retrospectively investigated the relationships between coagulation function, clinical stage and metastasis in patients with renal cell *** A total of 350 consecutive patients admitted to our Urology Department from 2004 to 2010 were diagnosed with renal cell carcinoma by histolopathologic examination and were included in this study. A total of 231 cases of renal benign tumors were considered as the control group. Fibrinogen, prothrombin time, activated partial thromboplastin time and international normalized ratio were evaluated in all subjects. Tumor size, clinical stage, lymph node metastasis, and distant metastasis were evaluated using radiologic imaging, intraoperative findings, and histological *** The preoperative plasma fibrinogen levels of patients with renal cell carcinoma （（383.9±146.7） mg/dl） were significantly higher than those of the control group （（316.7±62.0） mg/dl）（P ＜0.01）. We divided the renal cell carcinoma group into stages la, lb, Ⅱ, Ⅲ, and Ⅳ. The fibrinogen values were （315.6±64.6） mg/dl, （358.3±91.1） mg/dl, （465.6±164.7）mg/dl, （500.0±202.1） mg/dl, and （585.8±179.7） mg/dl, respectively. There were no significant differences in fibrinogen values between stage la and control groups. However, results of other stages showed significant differences when compared to control group values （P ＜0.01）. Using the cutoff value of 440 mg/dl, which defines hyperfibrinogenemia,plasma fibrinogen levels had a positive predictive value of 39.8% and a negative predictive value of 93.3% for predicting distant metastasis, with a sensitivity of 64.7% and specificity of 83.3%.Concluslons Preoperative plasma fibrinogen levels are elevated in patients with renal cell carcinoma with distant metastasis or lymph node metastasis. Potential metastasis is more likely if the

关键词： renal cell carcinoma fibrinogen metastasis hyperfibrinogenemia

Surgical management for carcinoma of the gallbladder： a single-institution experience in 25 years

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Chinese Medical Journal 2008年第19期121卷 1900-1905页

作者： LIANG Jian-wei dong shu-xiao ZHOU Zhi-xiang TIAN Yan-tao ZHAO dong-bing WANG Cheng-feng ZHAO Ping Department of Abdominal Surgical Oncology Cancer Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100021 China

Background Gallbladder carcinoma is rare and associated with dismal outcomes. Radical surgery is the only curative treatment, and options for adjuvant therapy remain limited. This study aimed to determine the factors influencing outcome of treatment in patients with gallbladder carcinoma, and to identify the patients who might benefit from radical surgery and adjuvant therapy. Methods Medical records and follow-up histories of 150 patients with gallbladder carcinoma who had undergone surgery between April 1980 and December 2005 were retrospectively reviewed. The factors predictive for the survival of the patients were identified using multivariate analysis. Results Surgery for gallbladder cancer was associated with an overall 5-year survival rate of 26.2%. After curative resection （40% of the patients）, the 5-year survival rate was 60.3%. The patients who underwent R0 resection had a significantly longer median survival （97.3 months） than those who had R1/R2 resection （8.3 months） or only laparotomy （3.7 months）（P 〈0.0001）. Univariate analysis showed that resectability, American Joint Committee on Cancer staging, tumor grade, adjuvant therapy, jaundice at presentation, depth of tumor invasion, lymph node involvement, distant metastasis, and carcinoembryonic antigen level were statistically significant predictors for survival. Multivariate analysis revealed American Joint Committee on Cancer staging and resectability were independent prognostic factors for survival. The patients who underwent noncurative resection might benefit from adjuvant therapy （median survival, 12.4 months vs 7.2 months, P=-0.006）. Conclusions Favorable survival rate can be achieved after curative resection, even for selected patients with advanced disease. Adjuvant therapy may improve the survival of patients with gallbladder carcinoma.

关键词： gallbladder carcinoma radical resection prognostic factor adjuvant therapy

Switchable dual-wavelength erbium-doped fiber laser with a tilted fiber grating

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Optoelectronics Letters 2007年第1期3卷 27-29页

作者： JIN Long KAI Gui-yun XU Ling-ling LIU Bo ZHANG Jian LIU Yan-ge YUAN shu-zhong dong xiao-yi Institute of Modern Optics Nankai University Tianjin 300071 China

A dual-wavelength erbium doped fiber laser with a tilted fiber Bragg grating and photonic crystal fiber is proposed and demonstrated. In the laser, a 2W EDFA provides gain for all the laser lines; the highly nonlinear photonic crystal fiber introduces dynamic energy transfer between the two wavelengths caused by four wave mixing effect, so that a stable dual- wavelength oscillation at room temperature is implemented. Different switching modes can be achieved by adjusting the lateral offset between the fiber grating and the guiding single mode fiber or by varying the state of polarization in the laser cavity. The maximum of output power of the laser has reached 314mW.

关键词：开关双波长铒掺杂光纤激光器倾斜光纤光栅光子晶体光纤

EGCG对乙酸诱导大鼠结肠炎的治疗作用及抗氧化机制

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世界华人消化杂志 2007年第4期15卷 359-364页

作者：冉志华陈迟萧树东上海交通大学医学院附属仁济医院消化内科上海市消化疾病研究所上海市200001

目的:在大鼠结肠炎的模型中,研究表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate, EGCG)的治疗作用及其抗氧化损伤作用的机制．方法:SD大鼠60只,随机分为正常对照组(n =10)、模型安慰剂组(n=20)、EGCG治疗组(n=15)、柳氮磺吡啶(S... 详细信息

目的:在大鼠结肠炎的模型中,研究表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate, EGCG)的治疗作用及其抗氧化损伤作用的机制．方法:SD大鼠60只,随机分为正常对照组(n =10)、模型安慰剂组(n=20)、EGCG治疗组(n=15)、柳氮磺吡啶(SASP)治疗组(n= 15)．正常组常规饲养,模型安慰剂组、EGCG组、SASP组80 g/L乙酸造模后分别予以生理盐水2 mL/d、EGCG 50 mg/(kg·d)、SASP 0．25 g/(kg·d)灌胃治疗7 d,观察大鼠活动状态,进食量,体质量,大便性状,大便出血情况,计算疾病活动指数(DAI),判断疗效．第8天处死大鼠并进行结肠黏膜损伤指数(CMDI)评分,组织学评级,测定组织一氧化氮自由基(NO)、脂质过氧化产物丙二醛(MDA)以及超氧化物歧化酶(SOD)含量．结果:与模型安慰剂组相比,EGCG显著改善DAI(1．1±0．9 vs 3．9±0．4,P＜0．01)、CMDI(1．5±0．9 vs 3．3±0．6,P＜0．05)和组织学评级(4．6±3．1 vs 9．3±2．8,P＜0．01)．与SASP组相比, EGCG显著改善DAI(1．1±0．9 vs 3．0±1．2, P＜0．01)、CMDI(1．5±0．9 vs 2．3±0．9,P＜0．05)和组织学评级(4．6±3．1 vs 7．9±4．0,P＜0．05)．与模型安慰剂组相比,EGCG组NO含量显著下降(9．1±5．6μmol/g vs 15．4±5．0μmol/g, P＜0．05),MDA含量也显著下降(0．9±0．6μmol/g vs 1．5±0．6μmol/g,P＜0．05),SOD含量显著提高(3090．6±568．4 nkat/mg vs 1373．6±410．1 nkat/mg,P＜0．05)．与SASP组相比,EGCG组SOD含量显著提高(3090．6±568．4 nkat/mg vs 1268．6±43 1．8 nkat/mjz,P＜0．05)．结论:EGCG可通过抑制氧化损伤减轻结肠炎症反应．且疗效优于传统药物SASP．

关键词：表没食子儿茶素没食子酸酯结肠炎抗氧化机制