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Treatment of pancreatic pseu do cysts in line with D'Egidio's classification

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World Journal of Gastroenterology 2005年第5期11卷 729-732页

作者： Ai-BinZhang shu-senzheng DepartmentofHepatobiliarySurgery FirstHospitalCollegeofMedicineZhejiangUniversityHangzhou310003ZhejiangProvinceChina

AIM: To explore the implications of underlying diseases in treatment of pancreatic pseudocysts (PPC). METHODS: Clinical data of 73 cases of pancreatic pseudocyst treated in a 12-year period were reviewed comprehensively. Pancreatic pseudocysts were classified according to the etiological criteria proposed by D'Egidio. The correlation between the etiological classification, measure of treatment and clinical outcome of the patients was analyzed. RESULTS: According to the etiological criteria proposed by D'Egidio, 73 patients were divided into three groups. Group I was comprised of 37 patients with type I pseudocyst, percutaneous drainage was successful in the majority (9/11, 82%) while external or internal drainage was not satisfactory with a low success rate (8/16, 50%). Group II was comprised of 24 patients with type II pseudocyst, and internal drainage was curative for most of the cases (11/12, 92%), but the success rate of percutaneous or external drainage was unacceptably low (4/9, 44%). Group III consisted of 12 patients with type III pseudocyst. Internal drainage or pancreatic resection performed in 10 of these patients produced a curative rate of 80% (8/10) with the correction of the ductal pathology as a prerequisite. CONCLUSION: The classification of pancreatic pseudocyst based on its underlying diseases is meaningful for its management. Awareness of the underlying diseases of pancreatic pseudocyst and detection of the ductal pathology in type II and III pancreatic pseudocysts with endoscopic retrograde cholangiopancreatography may help make better decisions of treatment to reduce the rate of complications and recurrence.

关键词： Pancreatic pseudocysts D'Egidio's classification

Inhibition of PMA-induced endothelial cell activation and adhesion by over-expression of domain negative IκBα protein

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World Journal of Gastroenterology 2005年第20期11卷 3080-3084页

作者： Jian-FengWei KeSun Shi-GuoXu Hai-YangXie shu-senzheng KeyLabofMulti-organTransplantationofMinistry TheFirstAffiliatedHospitalCollegeofMedicineZhejiangUniversityHangzhou310003ZhejiangProvinceChina DepartmentofPathology TheFirstAffiliatedHospitalCollegeofMedicineZhejiangUniversityHangzhou310003ZhejiangProvinceChina

AIM: NF-κB, regulate the expression of cytokine-inducible genes involving immune and inflammatory responses, will be potential therapy approach for allograft from rejection. In this study, we use pCMV-IκBαM vector to inhibit NF-κB activation and investigate the effect of pCMV-IκBαM in inhibition of T cells adhesion to endothelial cells. METHODS: The NF-κB activity was detected with pNF-κB reporter gene and electrophoretic mobility shift assay. Expression of cell surface molecules was detected by RT-PCR and flow cytometer. The cell-cell adhesion assay was performed to determine the effect of pCMV-IκBαM in inhibition of T cells adhesion to endothelial cells. RESULTS: We could find that NF-κB activity is inhibited by over-expression of non-degraded IκBα protein. Expression of adhesion molecules like ICAM-1, VCAM-1, and P-selectin as well as cell-cell adhesion were inhibited significantly by transfection of the pCMV-IκBαM vector. CONCLUSION: Our results indicate that the pCMVIκBαM, which inhibit the activity of NF-κB through over-expression of non-degraded IκBα protein, can be used for gene therapy in diseases involving NF-κB activation abnormally like organ transplantation via inhibiting cell adhesion.

关键词： Cytokine-inducible genes Endothelial cells

Possible causes of central pontine myelinolysis after liver transplantation

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World Journal of Gastroenterology 2004年第17期10卷 2540-2543页

作者： JunYu shu-senzheng Ting-BoLiang YanShen Wei-LinWang Qing-HongKe DepartmentofHepatobiliarySurgery theFirstAffiliatedHospitalMedicalSchoolofZhejiangUniversityHangzhou310003ZhejiangProvinceChina

AIM: To sum up the clinical characteristics of patients with central pontine myelinolysis (CPM) after orthotopic liver transplantation (OLT) and to document the possible causes of ***: Data of 142 patients undergoing OLT between January 1999 to May 2003 were analyzed *** risk factors during perioperation were analyzed in patients with and without CPM: primary liver disease,preoperative serum sodium level, magnesium level and plasma osrnolality, fluctuation degree of serum sodium concentration, and irnmunosuppressive drug level, ***: A total of 13 (9.2%) neurologic symptoms appeared in 142 patients post-operation including 5 cases (3.5%) with CPM and 8 cases (5.6%) with cerebral hemorrhage or infarct. Two patients developing CPM after OLT had a hyponatrernia history before operation (serum sodiurn<130 mmol/L), their mean serum sodium level was 130.6±5.54 mmol/L. The serum sodium level was significantly lower in CPM patients than in patients without neurologic complications or with cerebral hemorrhage/infarct (P<0.05).The increase in serum sodiurnduring perioperative 48 h after OLT in patients wibh CPM was significantly greater than bhat in patients with cerebral hemorrhage/infarct but without neurologic complications (19.5+6.54 retool/L, 10.1+6.43 retool/L,4.5±4.34 mmol/L, respectively, P<0.05). Plasma osrnolality was greatly increased postoperation in patients with *** was noted in all patients perioperation,but there were no significant differences between *** duration of operation on patients with CPM was longer than that on others (492+190.05 rnin, P<0.05). Cyclosporin A (CsA) levels were normal in all patients, but there were significant differences between patients with or without neurologic complications (P<0.05).CONCLUSION: CPM may be more prevalent following liver transplantation. Although the diagnosis of CPM after OLT can be made by overall neurologic evaluations including magnetic resonance imag

关键词：可能性作用脑桥肝移植消化系统 CPM

Effects of intestinal lymph on expression of neutrophil adhesion factors and lung injury after trauma-induced shock

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World Journal of Gastroenterology 2004年第21期10卷 3221-3224页

作者： Zuo-BingChen shu-senzheng GaoYuan Chen-YanDing YunZhang Xue-HongZhao Lin-MeiNi DepartmentofEmergencySurgery.FirstAffiliatedHospital ZhejiangUniversitySchoolofMedicineHangzhou310003ZhejiangProvinceChina

AIM: To study how intestinal lymph after trauma-induced shock (TIS) interferes with expression of neutrophil adhesion factors (CDllb and CD18) and causes lung ***: Thirty-two adult healthy Sprague-Dawley rats were randomly divided into four experimental groups. Groups 1 and 2 included rats with TIS caused by hitting the midupper part of both side femoral bones with a 2 500 kg rawiron, and with or without ligation of mesenteric lymph duct. Groups 3 and 4 included rats with sham-TIS and with or without ligation of mesenteric lymph duct. Expression of neutrophil CD18 and CD11b in at 1 and 3 h after a 90-min TIS/sham-TIS was evaluated. These rats were killed at 3 h after TIS/sham-TIS, and lungs were taken immediately. The main lung injury indexes (bhe MPO activity and lung injury score) were ***: The expressions of CD18 and CD11b at 1 and 3 h after a 90-min TIS and the main lung injury indexes were significantly increased compared with those in the sham-TIS groups (P<0.05). Moreover, at 1 and 3 h after TIS, the expressions of CD18 (32.12±1.25 and 33.46±0.98) and CD11b (29.56±1.35 and 30.56±1.85) were significantly decreased in rats with ligation of mesenteric lymph duct, compared with those (52.3±1.12 and 50.21±1.25, and 42.24±1.24 and 42.81±1.12, respectively) in those without the ligation (all P<0.05). The main lung injury indexes in rats with TIS with ligation .of mesenteric lymph duct (0.96±0.12 and 6.54±0.35) were also significantly decreased, compared with those (1.56±0.21 and 9.56±0.23) in rats with TIS without the ligation (both P<0.05). However, there was no significant difference in expressions of CD18 and CD11b and the main lung injury indexes between the two sham-TIS ***: Previous ligation of mesenteric lymph ducts prevents or alleviates the up-regulated expression of PMN CD18 and CD11b and the lung injury induced by TIS. Our findings also indicate that neutrophil adhesion molecule activation and lung injury during TIS a

关键词：肠内淋巴基因表达嗜中性支持因子肺损害外伤 TIS 肠系膜

Expression of growth hormone receptor and its mRNA in hepatic cirrhosis

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World Journal of Gastroenterology 2003年第4期9卷 765-770页

作者： Hong-TaoWang shuangChen JieWang Qing-JiaOu ChaoLiu shu-senzheng Mei-HaiDeng Xiao-PingLiu DepartmentofHepato-biliarySurgery SunYat-SenMemorialHospitaltheSecondAffiliatedHospitalSunYat-SenUniversityGuangzhou510120GuangdongProvinceChina DepartmentofGastroenteralSurgery SunYat-SenMemorialHospitaltheSecondAffiliatedHospitalSunYat-SenUniversityGuangzhou510120GuangdongProvinceChina MolecularMedicalLaboratoryCenter SunYat-SenMedicalCollegeSunYat-SenUniversityGuangzhou510089GuangdongProvinceChina DepartmentofGeneralSurgery theThirdAffiliatedHospitalSunYat-SenUniversityGuangzhou510630GuangdongProvinceChina DepartmentofGeneralSurgery ShenzhenCentralHospitalPekingUniversityShenzhen518036China

AIM: To investigate the expression of growth hormone receptor (GHR) and mRNA of GHR in cirrhotic livers of rats with the intension to find the basis for application of recombinant human growth hormone (rhGH) to patients with liver ***: Hepatic cirrhosis was induced in SpragueDawley rats by administration of thioacetamide intraperitoneally for 9-12 weeks. Collagenase Ⅳ was perfused in situ for isolation of hepatocytes. The expression of GHR and its mRNA in cirrhotic livers was studied with radio-ligand binding assay, RT-PCR and digital image ***: One class of specific growth hormone-binding site, GHR, was detected in hepatocytes and hepatic tissue of cirrhotic livers. The binding capacity of GHR (RT, fmol/mg protein) in rat cirrhotic liver tissue (30.8±1.9) was significantly lower than that in normal control (74.9±3.9) at the time point of the ninth week after initiation of induction of cirrhosis (n=10, P＜0.05), and it decreased gradually along with the accumulation of collagen in the process of formation and development of liver cirrhosis (P＜0.05). The number of binding sites (×10 4/cell) of GHR on rat cirrhotic hepatocytes (0.86±0.16) was significantly lower than that (1.28±0.24)in control (n= 10, P＜0.05). The binding affinity of GHR among liver tissue, hepatocytes of various groups had no significant difference (P＞0.05). The expression of GHR mRNA (riOD,pixel) in rat cirrhotic hepatic tissues (23.3±3.1) was also significantly lower than that (29.3±3.4) in normal control (n=10, P＜0.05).CONCLUSION: The growth hormone receptor was expressed in a reduced level in liver tissue of cirrhotic rats,and lesser expression of growth hormone receptors was found in a later stage of cirrhosis. The reduced expression of growth hormone receptor was partly due to its decreased expression on cirrhotic hepatocytes and the reduced expression of its mRNA in cirrhotic liver tissue.

关键词：生长激素受体肝硬化 mRNA 逆转录聚合酶链反应慢性肝病免疫反应

Specific COX-2 inhibitor NS398 induces apoptosis in human liver cancer cell line HepG2 through BCL-2

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World Journal of Gastroenterology 2005年第2期11卷 204-207页

作者： Dong-ShengHuang Ke-ZhenShen Jian-FengWei Thng-BoLiang shu-senzheng Hai-YangXie DepartmentofHepatobiliaryPancreaticSurgery TheFirstAffiliatedHospitalZhejiangUniversityCollegeofMedicineHangzhou310003ZhejiangProvinceChina

AIM: To evaluate the effects of NS-398, a cyclooxygenase-2 (COX-2) inhibitor, on the proliferation and apoptosis of HepG2 cells. METHODS: The effects of NS-398 on the proliferation of HepG2 cells were evaluated by MTT. DNA fragmentation gel analysis was used to analyze the apoptotic cells. DNA ploidy and apoptotic cell percentage were calculated by flow cytornetry. The expression of COX-2 and Bcl-2 mRNA was identified by competitive RT-PCR. Furthermore, expression level of Bcl-2 was detected using Western blot in HepG2 after treated with NS-398. RESULTS: NS-398 inhibited cell proliferation and induced apoptosis of HepG2 cells in a concentration-dependent manner. DNA ploidy analysis showed that S phase cells were significantly decreased with increase of NS-398 concentration. The quiescent GO/G1 phase was accumulated with decrease of Bcl-2 mRNA. Whereas NS-398 had no effect on the expression of COX-2 mRNA, and no correlations were found between COX-2 mRNA and HepG2 cell proliferation and apoptosis induced by NS-398 (r=0.056 and r=0.119, respectively). Bcl-2 protein level was inhibited after treated with NS-398. CONCLUSION: NS-398 significantly inhibits the proliferation and induces apoptosis of HepG2 cells. Mechanisms involved may be accumulation of quiescent GO/G1 phase and decrease of Bcl-2 expression.

关键词： Liver cancer NS-398 Bcl-2 protein COX-2

Attenuation of graft ischemia-reperfusion injury by urinary trypsin inhibitor in mouse intestinal transplantation

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World Journal of Gastroenterology 2005年第11期11卷 1605-1609页

作者： Ji-RenYu ShengYan Xiao-SunLiu Yi-JunWu Pei-FengFu Li-HuaWu shu-senzheng DepartmentofSurgery theFirstAffiliatedHospitalCollegeofMedicineZhejiangUniversityHangzhou310003ZhejiangProvinceChina

AIM: Ischemia/reperfusion (I/R) injury is one of the major obstacles for intestinal transplantation (ITx). Urinary trypsin inhibitor (Ulinastatin, UTI) suppresses proteases and stabilizes lysosomal membranes. We supposed that Ulinastatin would diminish I/R injury of intestinal ***: UTI- treated group and untreated control group were investigated by histological assessment at 1.5, 4, 24, and 72 h after ITx. Myeloperoxidase (MPO)activity was used as the activity of neutrophils, and malondialdehyde (MDA) was used as an index of lipid peroxidation. TNFα and i-NOS mRNA expression in graft tissue were measured by semi-quantitative ***11b+ Gr1+ cells in graft lamina propria were analyzed by flow ***: Histological scores of the graft showed that the tissue injury was markedly attenuated by UTI treatment at different time points after ITx, with reduced MPO and MDA value in the grafts. The expression of TNFα and i-NOS mRNA was profoundly inhibited, while the infiltration of CD11b+ Gr1+ cells into the intestinal graft was decreased in UTI ***: Urinary trypsin inhibitor attenuates I/R injury in mouse intestinal transplantation by reducing monocytes infiltration and down-regulation of TNFα and i-NOS mRNA expression.

关键词： Ischemia/reperfusion injury Ulinastatin Intestinal transplantation

Liver fibrosis caused by choledocholith to regress after biliary drainage

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World Journal of Gastroenterology 2005年第13期11卷 2013-2015页

作者： Zuo-BingChen shu-senzheng Guo-ZhiHu YuanGao Chen-YanDing YunZhang Xue-HongZhao Lin-MeiNi DepartmentofSurgery FirstAffiliatedHospitalZhejiangUniversitySchoolofMedicineHangzhou310003ZhejiangProvinceChina DepartmentofSurgery ZhujiCountyPeoples'HospitalZhuji311800ZhejiangProvinceChina

AIM: To study the correlation between liver flbrosis severity and biliary drainage in patients with choledocholith. METHODS: A follow-up study on seven patients with liver fibrosis due to choledocholith was made. The data, including biochemical tests (aspartate aminotransferase, alanine aminotransferase) and liver histological features before and after biliary drainage, were collected and studied. The fibrosis severity was scored on a scale from 0 to 3, with 0 denoting none, 1 portal and periportal fibrosis, 2 the presence of numerous fiber septa, and 3 cirrhosis. The average liver fibrosis severity scores of the first and second biopsy were compared with statistical method. RESULTS: The first, second liver fibrosis severity scores of these seven patients were 2,1; 2,1; 1,0; 1,1; 2,1; 2,1; 1,0 respectively. The results showed that the average liver fibrosis severity score of the second liver biopsy decreased significantly compared with the first liver biopsy (n=7,t=4.25,P<0.05). CONCLUSION: Liver fibrosis due to choledocholith may regress after biliary drainage.

关键词： Liver fibrosis Regress Biliary drainage

Combination of genistein with ionizing radiation on andro-gen-independent prostate cancer cells

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Asian Journal of Andrology 2004年第4期6卷 285-290页

作者： Sen-XiangYan YasuoEjima RyoheiSasaki shu-senzheng YusukeDemizu ToshinoriSoejima KazuroSugimura DepartmentofRadiology FirstAffiliatedHospitalCollegeofmedicineZhejiangUniversityHangzhou310003China DivisionofRadiology KobeGraduateSchoolofMedicineHyogo6500017Japan

Aim: To study the effect of the combined use of genistein and ionizing radiation (IR) on prostate DU145 cancer cells. Methods: DU145, an androgen-independent human prostate cancer cell line, was used in the experiment. Clonogenic assay was used to compare the survival of DU145 cells after treatments with genistein alone and in combination with graded IR. Apoptosis was assayed by DNA ladder and TUNEL stain. Cell cycle alterations were observed by flow cytometry and related protein expressions by immunoblotting. Results: Clonogenic assay demonstrated that genistein, even at low to medium concentrations, enhanced the radiosensitivity of DU145 cells. Twenty-four hours after treatment with IR and/or genistein, apoptosis was mainly seen with genistein at high concentrations and was minimally related to IR. At 72 h, apoptosis also occurred in treatment with lower concentration of genistein, especially when combined with IR. While both IR and genistein led to G2/M cell cycle arrest, combination of them further increased the DU145 cells at G2/M phase. This Gz/M arrest was largely maintained at 72 h, accompanied by increasing apoptosis and hyperdiploid cell population. Cell-cycle related protein analysis disclosed biphasic changes in cyclin B1 and less dramatically cdc-2, but stably elevated p21cipl levels with increasing genistein concentrations. Conclusion: Genistein enhanced the radiosensitivity of DU145 prostate cancer cells. The mechanisms might be involved in the increased apoptosis, prolonged cell cycle arrest and impaired damage repair.

关键词： prostate cancer genistein ionizing radiation (IR) apoptosis cell cycle