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Active compounds in RenShenJian decoction ameliorate insulin resistance in vitro

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Traditional Medicine Research 2022年第4期7卷 8-16页

作者： Zhen-Hua Lan Lan-Fang Tan Guo-En wang Meng-Qiu Tang Ruo-Hong wang shu-mei wang Guangdong Pharmaceutical University Guangzhou 510006China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine and Engineering&Technology Research Center for Chinese Materia Medica Quality of Guangdong Province Guangdong Pharmaceutical UniversityGuangzhou 510006China.

Background:RenShenJian decoction,a combination of Pueraria lobata(Willd.)Ohwi and Panax ginseng ***,has been used in China since the Song Dynasty(960-1279 C.E.)to relieve symptoms of diabetes ***,the key compounds in RenShenJian that ameliorate insulin resistance remain *** study identified the anti-diabetic compounds in RenShenJian by rescuing the decreased function of adenosine 5’-monophosphate-activated protein kinase(AMPK),sirtuin 3(SIRT3),or glucose transporter isoform 4(GLUT4).Methods:After streptozotocin-induced diabetic mice were treated with RenShenJian,fasting blood glucose levels and protein expression of SIRT3,p-AMPK,and AMPK were *** from RenShenJian in plasma were monitored using multiple responses by liquid chromatography-mass ***,two insulin-resistant cell models were incubated with compounds identified in RenShenJian in the *** uptake was determined using the fluorescent analog 2-(N-(7-nitrobenz-2-oxa-1,3-dia-xol-4-yl)amino)-*** expression levels of p-AMPK,AMPK,SIRT3,and GLUT4 were detected by western ***:RenShenJian decreased FBG levels and upregulated SIRT3 expression and AMPK phosphorylation in diabetic *** RenShenJian extracts were identified in the blood,11 of which increased the ratios of 2-(N-(7-nitrobenz-2-oxa-1,3-dia-xol 4-yl)amino)-2-deoxyglucose uptake in two insulin-resistant cell *** extracts increased AMPK phosphorylation,nine increased SIRT3 expression,and six elevated GLUT4 expression in palmitate-induced HepG2 *** extracts-puerarin,puerarin 6″-O-xyloside,genistein,ginsenoside Rb1,and ginsenoside Rd-simultaneously activated AMPK,SIRT3 and ***:A series of compounds in RenShenJian that target AMPK,SIRT3,and/or GLUT4 was confirmed and indicate the chemical material basis of amelioration of insulin resistance by RenShenJian.

关键词： SIRT3 AMPK GLUT4 diabetes Pueraria lobata(Willd.)Ohwi Panax ginseng C.A.Mey

Identification of Dual Receptor-binding Specific Strains of Human H5N1 Viruses in China

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Biomedical and Environmental Sciences 2012年第1期25卷 104-108页

作者： ZHOU Jian Fang ZOU shu mei LI Zi wang Min DONG Jie GUO Jun Feng WEI He Jiang WEN Le Ying XU Hong shu Yue Long State Key Laboratory for Molecular Virology and Genetic Engineering National Institute for Viral Disease Control and Prevention China CDC Beijing 102206 China

Objective Both the 2, 6 linkage and its topology on target cells are critical for the recognition by human influenza virus. The binding preference of avian flu virus H5N1 HA to the 2, 3-1inked sialylated glycans is considered the major factor limiting its efficient infection and transmission in humans. To monitor potential adaptation of H5N1 virus in human population, the surveillance of receptor-binding specificity was undertaken in China. Methods The binding specificity of 32 human H5N1 virus strains isolated from 2003 to 2009 was tested by 2, 3-specific sialidase-treated chicken red blood cell （CRBC） agglutination assay and a solid-phase direct binding assay with synthetic sialylglycopolymers. Results Dual binding preference to 2, 3 and 2, 6-glycans were found in two strains： A/Guangdong/1/06 （A/GD/1/06） and A/Guangxi/1/08 （A/GX/1/08）. Though minor effect of short-2, 6-binding was detected in A/GX/1/08 at a low virus titer, both showed high affinity to the oligosaccharide at a high load. Notably both are of the long-2, 6-recognition, with the same topology as that of human H1N1 and H3N2 viruses. Conclusion The findings suggest that human H5N1 virus in China likely acquired the potential human-adaptation ability. Further research and surveillance on receptor-binding specificity of H5N1 viruses are required.

关键词： Receptor-binding specificity Human H5N2 virus China

Combined Detection of Serum Matrix Metalloproteinase 9,Acetyl Heparinase and Cathepsin L in Diagnosis of Ovarian Cancer

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Chinese Journal of Cancer Research 2012年第1期24卷 67-71页

作者： Wei Zhang Xiao-xia Hu Xing-zhi Yang Qi wang Hong Cheng shu-mei wang Yan-ling Hu Zhi-jun Yang Li Li Department of Gynecologic Oncology Cancer Institute and HospitalGuangxi Medical UniversityNanning 530021China Department of Gynecology and Obstetrics the People's Hospital of Guangxi ProvinceNanning 530022China

Objective： To investigate the clinic values of combining test of serum matrix metalloproteinase 9 （MMP-9）, acetyl heparinase （Hpa） and Cathepsin L （CL） in diagnosis of ovarian cancer. Methods： Serum levels of MMP-9, Hpa and CL were detected in a total of 418 cases, including 217 cases with ovarian malignant tumor, 100 cases with ovarian benign tumor and 101 healthy controls, by using enzyme-linked immunosorbent assay （ELISA）. Their correlation with clinicopathologic feature of ovarian malignant tumor was analyzed and their diagnosis performance was evaluated by receiver operating characteristic （ROC）. The combined diagnosis model was established by logistic regression analysis. Results： The serum levels of MMP-9, Hpa and CL were significantly higher in patients with ovarian malignant tumor than in benign tumor and healthy control, the serum levels of CL and Hpa were higher in epithelial cancer than in non-epithelial tumor, and MMP-9, Hpa and CL were elevated in low grade and advanced stage compared to high grade and early stage. The sensitivity for diagnosis of ovarian malignant tumor from high to low was CL, Hpa and MMP-9, and the specificity was MMP-9, CL and Hpa. The united diagnosis model was established and showed the sensitivity and specificity of combined detection were 84.6% and 82.1%, respectively, which were significantly higher than a single tumor marker. Conclusion： Serum MMP-9, Hpa and CL were correlated with ovarian malignant tumor and the combined detection of which may be valuable for clinical diagnosis of ovarian malignant tumor.

关键词： Ovarian cancer Matrix metalloproteinase 9 Acetyl heparinase Cathepsin Diagnosis

Optimization of synergistic drug combinations of different sparganins against blood stasis syndrome

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Traditional Medicine Research 2021年第6期6卷 24-34页

作者： Huan-Zheng Yu Jia-Le Cai Su-Long Ji Ming-Hua Xian Sheng-wang Liang shu-mei wang School of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006China Engineering&Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province Guangdong Pharmaceutical UniversityGuangzhou 510006China School of Pharmacy Guangdong Pharmaceutical UniversityGuangzhou 510006China

Background:Blood stasis syndrome is one of the major syndromes in the development of chronic conditions in traditional Chinese *** stasis syndrome is closely related to microcirculation dysfunction and ***,as a representative traditional Chinese medicine for promoting blood circulation and removing blood stasis,there are many studies on its anticoagulant,antiplatelet aggregation and antithrombotic ***:Optimization of sparganin compounds,comprising sparganin A,sparganin B,and sparganin C for the characterization of drug pair effects against blood stasis syndrome was carried *** quadratic regression orthogonal combination design was carried out in a mouse model of blood stasis *** concentrations of thromboxane B2,plasminogen activator inhibitor 1,fibrinogen,and endothelin 1 were evaluated by enzyme linked immunosorbent ***,hepatic,and spleen indices were also *** outcomes of the evaluations aided in identifying optimum parameter values for the most favorable precipitation against blood stasis ***,Cytoscape 3.7.1 was used for network pharmacology to predict the key targets of sparganins and ***:After sparganin A,sparganin B,and sparganin C treatment,hepatic had a downward trend,while spleen indices and thymus had an upward *** with the model group,thromboxane B2,endothelin 1,plasminogen activator inhibitor 1,and fibrinogen were decreased(P<0.05).The blood stasis syndrome model regression equation was extremely significant,and the correlation coefficient of R was 0.939,indicating that sparganin A,sparganin B and sparganin C were positively correlated at all *** to the dosage of these three factors and test results,the equation was fitted and the maximum values of the three sparganin in the corresponding dose range were obtained as follows:A=0.2982 mg/10g,B=0.1438 mg/10g,C=0.0417 mg/***,the optimum parameters were found to be 0.298:0.144

关键词： Rhizoma Sparganii sparganins blood stasis syndrome ternary quadratic regression orthogonal combination design network pharmacology

Integrated network pharmacology and metabolomics to explore the mechanisms of Shenzao dripping pill against chronic myocardial ischemia

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Traditional Medicine Research 2024年第11期9卷 12-26页

作者： Jie-Hui Kuang Tao Hu Lu-Yong Zhang Yu-Feng Yao Ming-Hua Xian shu-mei wang School of Chinese Materia Medica Guangdong Pharmaceutical UniversityGuangzhou 510006China Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006China Engineering&Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province Guangdong Pharmaceutical UniversityGuangzhou 510006China Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems Guangdong Pharmaceutical UniversityGuangzhou 510006China Traditional Chinese Medicine Resource Germplasm Bank Management Center Yunfu 527500China.

Background:Shenzao dripping pill(SZDP)is empirically prescribed for treating cardiac ***,there is a lack of comprehensive knowledge regarding the underlying mechanisms contributing to its therapeutic *** objective of this study is to investigate the underlying mechanism of SZDP against chronic myocardial ischemia(CMI)in a rat ***:In this study,we utilized electrocardiographic and echocardiographic detection along with pathological tissue analysis to evaluate the efficacy of *** integration of network pharmacology and metabolomics was conducted to investigate the *** docking and molecular dynamics simulations were used to validate the binding energy between the compounds of SZDP and the associated ***:The results showed that SZDP was able to improve T wave voltage,reverse CMI abnormalities in ejection fraction and fractional shortening,and restore histopathological heart *** results indicated that disturbances of metabolic profile in CMI rats were partly corrected after SZDP administration,mainly affecting purine metabolism.13-Docosenamide may be the potential metabolic biomarker of the therapeutic application of SZDP for *** network pharmacology and metabolomics,thiopurine S-methyltransferase(TPMT),xanthine dehydrogenase/oxidase(XDH),bifunctional purine biosynthesis protein ATIC(ATIC),and cytochrome p4501A1(CYP1A1)were identified as possible targets of SZDP to exert therapeutic effects by enhancing the metabolic levels of L-Tryptophan,Deoxyribose 1-phosphate and Phosphoribosyl ***:SZDP has a therapeutic effect on CMI by regulating metabolite levels,acting on the targets of TMPT,XDH,ATIC,and CYP1A1,and reducing cardiomyocyte injury and myocardial fibrosis.

关键词： chronic myocardial ischemia metabolomics network pharmacology serum metabolites Shenzao dripping pill

Analysis on Factors Related to Rabies Epidemic in China from 2007-2011

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Virologica Sinica 2012年第2期27卷 132-143页

作者： Cui-ping Yin Hang Zhou Hui Wu Xiao-yan Tao Simon Rayner shu-mei wang Qing Tang Guo-dong Liang Institute for Viral Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Epidemiology and Health Statistics School of Public HealthShandong UniversityJinan 250012China Chinese Center for Disease Control and Prevention Beijing 102206China State Key Laboratory of Virology Wuhan Institute of VirologyChinese Academy of SciencesWuhan 430071China

To analyze features of the rabies epidemic in China between 2007 and 2011, identify factors influencing the epidemic and to provide a scientific basis for further control and prevention of rabies, Descriptive epidemiological methods and statistical analysis was used on data collected from the National Disease Reporting Information System between 2007 to 2011 and the National Active Surveillance System between 2007 and 2010. Our analysis shows that while the number of human rabies cases decreased year by year, the number of districts reporting cases did not show significant change. The situations in Guangdong, Guangxi, Guizhou and Hunan provinces clearly improved over the period but they remain provinces with high-incidence, and consequently influence the epidemic situation of surrounding provinces and possibly the whole country. Summer and autumn were high-incidence seasons. Farmers, students and pre-school children represent the high-risk populations, and rates of cases in farmers increased, those for students decreased, and pre-school children remained unchanged. Provinces with active surveillance programs reported a total of 2346 individual cases, of which 88.53% were associated with canines. Postexposure prophylaxis （PEP） of rabies cases was not significantly improved, whereas PEP in post-exposure population was good. In rural regions of China, canine density was reduced somewhat, and the immunization rate increased slightly. Finally we show that while the epidemic decreased 2007 to 2011 in China, cases continued to be diffused in certain regions. Lack of standardization of PEP on rabies eases was the main reason of morbidity. The high density and low immunization of dog in rural areas and the defective situation of PEP are still continuous occurrences in China and remain a cause for concern.

关键词： Rabies Surveillance Epidemic factor

Integrated UHPLC-MS and network pharmacology to explore the active constituents and pharmacological mechanisms of Shenzao dripping pills against coronary heart disease

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Traditional Medicine Research 2022年第3期7卷 68-80页

作者： Tao Hu Ke-Ning Zheng Jia-Yin Liang Dan Tang Lu-Yong Zhang Ming-Hua Xian shu-mei wang Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006China Engineering&Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province Guangzhou 510006China School of Traditional Chinese Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006China Guangzhou Key Laboratory of Construction and Application of New Drug Screening Model Systems Guangdong Pharmaceutical UniversityGuangzhou 510006China School of Pharmacy Guangdong Pharmaceutical UniversityGuangzhou 510006China

Background:Shenzao dripping pills(SZDP)is an empirical prescription of traditional Chinese medicine that is mainly used to treat coronary heart ***,the chemical composition and pharmacological mechanisms of SZDP are ***:In this study,ultra-high performance liquid chromatography-quadruple-Exactive Orbitrap mass spectrometry was used to identify the chemical components in extracts and medicated plasma of ***,we performed network pharmacology methods,including target prediction by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform and Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine,protein-protein interaction network via STRING database;further,the key targets and compounds were screened using ***,the key targets and compounds were validated by molecular ***:72 chemical constituents were identified from SZDP by high performance liquid chromatography and mass spectrometry *** the components absorbed into plasma by SZDP,24 prototype components and 9 metabolized components were *** network pharmacology analysis of the prototype components showed that there are 13 key compounds(including ginsenoside Rc,Rb1,Rb2,ferulic acid,etc.),90 proteins(including proto-oncogene tyrosine-protein kinase Src,nuclear receptor subfamily 3 group C member 1,caspase-3,etc.),and 10 pathways(including estrogen,IL-17 and VEGF signaling pathway,etc.)that play an essential role in the treatment of coronary heart disease with *** addition,the results of molecular docking revealed that ginsenosides Rc,Rb2 and Rb1 have strong binding activities to the caspase-3,as well as ginsenoside Rb2 to the nuclear receptor subfamily 3 group C member ***:This study showed that SZDP might act through multiple chemical constituents and targets against coronary heart disease.

关键词： Shenzao dripping pills coronary heart disease chemical constituents network pharmacology molecular docking

Effects of Melatonin Levels on Neurotoxicity of the Medial Prefrontal Cortex in a Rat Model of Parkinson＇s Disease

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Chinese Medical Journal 2017年第22期130卷 2726-2731页

作者： Yan Li shu-mei wang Lei Guo Jian Zhu Ying wang Lei Li Yan-Xin Zhao Department of Neurology Jinan Central Hospital Affiliated to Shandong University Jinan Shandong 250013 China General Medical Teaching and Research Section Jinan Central Hospital Affiliated to Shandong University Jinan Shandong 250013 China Department of Medical Imaging Jinan Central Hospital Affiliated to Shandong University Jinan Shandong 250013 China Department of Medical Imaging Shandong Provincial Hospital Jinan Shandong 250014 China

Background： Damage of the medial prefrontal cortex （mPFC） results in similar characteristics to the cognitive deficiency seen with the progress of Parkinson＇s disease （PD）. Since the course of mPFC damage is still unclear, our study aimed to investigate the effects of melatonin （MT） on neurotoxicity in the mPFC of a rat model of PD. Methods： One hundred and fifty-four normal, male Wistar rats were randomly divided into the following five groups： normal ＋ normal saline （NS）, normal ＋ 6-hydroxydopamine （6-OHDA）, sham pinealectomy （PX）＋ 6-OHDA, PX ＋ 6-OHDA, and MT ＋ 6-OHDA. 6-OHDA was injected into the right substantia nigra pars compacta （SNc） and ventral tegmental area （VTA） of each group, except normal ＋ NS, 60 days after the PX. In the MT treatment group, MT was administered immediately after the intraperitoneal injection at 4 p.m. every day, for 14 days. Neuronal apoptosis in the mPFC was exalnined using the TUNEL method, while the expression oftyrosine hydroxylase （TH）, Bax, and Bcl-2 in this region was measured using immunohistochemistry. The concentration of malondialdehyde （MDA） in the mPFC was examined using the thioharbituric acid method. Results： Rats in the normal ＋ 6-OHDA and sham PX ＋ 6-OHDA groups were combined into one group （Group N ＋ 6-OHDA） since there was no significant discrepancy between the groups for all the detected parameters. Apoptosis of cells in the NS, MT ＋ 6-OHDA, N ＋ 6-OH DA, and PX ＋ 6-OHDA groups was successively significantly increased （Hc = 256.25, P 〈 0.001 ）. The gray value of TH （＋） fibers in the NS, MT ＋ 6-OHDA, N ＋ 6-OHDA, and PX ＋ 6-OHDA groups was also successively significantly increased （F= 99.33, P 〈 0.001 ）. The staining intensities of Bax and Bcl-2 were as follows： Group NS ＋/＋, Group MT ＋ 6-OHDA ＋＋/＋, Group N ＋ 6-OHDA ＋＋/＋, and PX ＋ 6-OHDA ＋＋＋/＋. The concentrations of MDA in the NS, MT ＋ 6-OHDA, N ＋ 6-OHDA, and PX ＋ 6-OHDA groups were significantly increased in sequence （Hc = 296.309, P 〈 0.001 ）. Conclusions： Neuronal

关键词： Medial Prefrontal Cortex Melatonin Pinealectomy Tyrosine Hydroxylase Ventral Tegmental Area

Neuroprotective effect and mechanism of daidzein in oxygen-glucose deprivation/reperfusion injury based on experimental approaches and network pharmacology

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Traditional Medicine Research 2021年第5期6卷 10-19页

作者： Ming-Hua Xian Si-Kai Zhan Ke-Ning Zheng Qu-liu Ke-Ning Li Jia-Yin Liang shu-mei wang Key Laboratory of Digital Quality Evaluation of Chinese Materia Medica of State Administration of Traditional Chinese Medicine Guangzhou 510006China Engineering&Technology Research Center for Chinese Materia Medica Quality of the Universities of Guangdong Province Guangzhou 510006China School of Traditional Chinese Pharmacy Guangdong Pharmaceutical UniversityGuangzhou 510006China School of Pharmacy Guangdong Pharmaceutical UniversityGuangzhou 510006China School of Traditional Chinese Pharmacy China Pharmaceutical UniversityNanjing 211198China

Background:Daidzein,phytoestrogens derived from the Pueraria lobata(Willd.)Ohwi root used in traditional Chinese medicine,has a wide range of biological activities,including antioxidant,anti-inflammatory,and ***,the neuroprotective role of daidzein in oxygen-glucose deprivation/reperfusion injury and its underlying mechanism are still ***:In this study,we used pheochromocytoma cells induced by oxygen-glucose deprivation and reperfusion to study the potential effect in the protection of the nerve ***,we used molecular docking simulation and network pharmacology to predict the possible targets and pharmacological pathways of *** blot was used to verify the expression of target proteins with or without adding the ***:After daidzein treatment,cell vitality had an upward trend(P<0.05)and the release of lactate dehydrogenase had a downward trend(P<0.01)in dose-dependent compared with the model group by exposure to oxygen-glucose deprivation and *** core targets were analyzed through network pharmacology and molecular docking including catalase,peroxisome proliferator-activated receptor gamma,vascular endothelial growth factor A,interleukin-6,tumor necrosis factor,nitric oxide synthase 3,prostaglandin-endoperoxide synthase 2,and RAC-alpha serine/threonine kinase *** results suggest that catalase may be a first-ranked target for the neuroprotective role of *** Ontology enrichment analysis indicated the pathways mainly contained molecule metabolic process,while Kyoto Encyclopedia of Genes and Genomes enrichment analysis focus on pathways in terms of inflammation such as tumor necrosis factor signal ***,Western blot results showed that daidzein had a significant increase on the expression of protein catalase(P<0.01).Daidzein reversed catalase level alterations after oxygen-glucose deprivation reperfusion injury in a dose-dependent manner which was consistent with the cata

关键词： daidzein neuroprotection catalase oxygen-glucose deprivation and reperfusion network pharmacology