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The expression of Usp26 gene in mouse testis and brain

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Asian Journal of Andrology 2009年第4期11卷 748-749页

作者： Jie Zhang Hong Tian Yong-Wei Huo Dang-Xia Zhou Hai-Xu Wang Li-Rong Wang qiu-Yang Zhang shu-dong qiu Department of Anatomy Histology & Embryology Medical College of Xi'an Jiaotong University Xi'an 710061 China.

In this article, published on 1 July 2009, the following colored figures were wrongly published in black and white Asian Journal of Andrology would like to apologize to the authors and readers for the error and any c... 详细信息

关键词： expression Usp26 gene mouse testis brain

NT4(Si)-p53(N15)-antennapedia induces cell death in a human hepatocellular carcinoma cell line

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World Journal of Gastroenterology 2009年第46期15卷 5813-5820页

作者： Li-Ping Song Yue-Ping Li Ning Wang Wei-Wei Li Juan Ren shu-dong qiu Quan-Ying wang Guang-Xiao Yang Department of Radiotherapy Oncology First Hospital MedicalSchool of Xi'an Jiao Tong University Xi'an 710061 ShaanxiProvince China Center for Reproductive Medicine AffiliatedHospital of Hainan Medical College Haikou 570102 HainanProvince China Department of Anatomy and Histology &Embryology Medical school of Xi'an Jiao Tong UniversityXi'an 710061 Shaanxi Province China Xi'an HuaguangBioengineering Co Xi'an 710061 Shaanxi Province China

AIM： To construct the recombinant lentivirus expression plasmid, pLenti6/V5-NT4 p53（N 15）-antennapedia （Ant）, and study its effect on HepG2 cells. METHODS： Plasmid pLenti6/V5-NT4 p53（N15）-Ant was constructed incorporating the following functional regions, including signal peptide sequence and proregion of neurotrophin 4, N-terminal residues 12-26 of p53 and 17 amino acid drosophila carrier protein, Ant. Hepatocellular carcinoma （HepG2） cells were used for transfection. 3-[4,5-climethyl-thiazol-2yl]-2,5 diphenyl tetrazolium bromide （MI-I） assay, lactate dehydrogenase （LDH） release assay, transmission electron microscopy （TEM） and flow cytometric analysis （FCM） were employed to investigate the effects of LV-NT4（Si）- p53（N15）-Ant in vitro on HepG2 cells. In vivo experiment was also performed to investigate the inhibitory effect of LV-NT4（Si）-p53（N15）-Ant on tumor growth in nude ***： LV-NT4（Si）-p53（N15）-Ant significantly suppressed the growth of HepG2 cells. MTT assay showed that the growth of HepG2 cells was mucj more significantly inhibited by LV-NT4（Si）-p53（N15）-Ant than by LV-EGFP. The inhibition rate for HepG2 cell growth in the two groups was 46.9% and 94.5%, respectively, 48 h after infection with LV-NT4（Si）-p53（N15）-Ant, and was 33.9% and 95.8%, respectively, 72 h after infection with LV-NT4（Si）-p53（N15）-Ant （P 〈 0.01）. Light microscopy and TEM showed morphological changes in HepG2 cells infected with LV-NT4（Si）-p53（N15）-Ant, but no significant changes in HepG2 cells infected with LV-EGFR Changes were observed in ultra-structure of HepG2 cells infected with LV-NT4（Si）-p53（N15）-Ant, with degraded membranes, resulting in necrosis. LDH release from HepG2 cells was analyzed at 24, 48, 72 and 96 h after infection with LV-NT4（Si）-p53（N15）-Ant and LV-EGFP, which showed that LDH release was significantly higher in LV-NT4（Si）-p53（N15）-Ant treatment group （682 IU/L） than in control group （45 IU/L, P 〈 0.01）. The longer the time was after infection, the bigger the

关键词： Gene therapy Lentivirus vector Anticancer Necrosis LV-NT4(Si)-p53(N15)-Ant Hepatocellularcarcinoma cell line

Novel mutations in ubiquitin-specific protease 26 gene might cause spermatogenesis impairment and male infertility

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Asian Journal of Andrology 2007年第6期9卷 809-814页

作者： Jie Zhang shu-dong qiu Sheng-Bin Li Dang-Xia Zhou Hong Tian Yong-Wei Huo Ling Ge qiu-Yang Zhang Department of Anatomy Histology and Embryology Research Center of Reproductive Medicine Department of Forensic Medicine Department of Pathology Medical College of Xi＇ an Jiaotong University Xi＇ an 710061 China

Aim： To study the incidence of single nucleotide polymorphisms in ubiquitin-specific protease 26 （USP26） gene and its involvement in idiopathic male infertility in China. Methods： Routine semen analysis was performed. Infertility factors such as immunological, infectious and biochemical disorders were examined to select patients with idiopathic infertility. DNA was isolated from peripheral blood of the selected patients and control population, which were examined for mutations using polymerase chain reaction-single strand conformation polymorphism analysis. Furthermore, nucleotide sequences were sequenced in some patients and controls. Results： Of 41 infertile men, 9 （22.0%, P = 0.01） had changes in USP26 gene on the X chromosome. A compound mutation （364insACA; 460G→A） was detected in 8 patients （19.5%, P = 0.01） and a 1044T→A substitution was found in 1 patient （2.4%, P 〉 0.05）. All three variations led to changes in the coding amino acids. Two substitutions predict some changes： 460G→ A changes a valine into an isoleucine, and 1044T → A substitutes a leucine for a phenylalanine. Another insertion of three nucleotides ACA causes an insertion of threonine. No other changes were found in the remaining patients and fertile controls. Conclusion： The USP26 gene might be of importance in male reproduction. Mutations in this gene might be associated with male infertility, and might negatively affect testicular function. Further research on this issue is in progress.

关键词： male infertility deubiquitination enzymes ubiquitin-specific protease 26

The immunotherapeutic effects of ginsenoside Ro in mice with diabetes mellitus

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The immunotherapeutic effects of ginsenoside Ro in mice with...

第十五届国际药理学大会

作者： Ru- Jiang LI shu - dong qiu. Department of Histology and Embryology Medical School of Xi’ an Jiaotong University Xi’ an710061 China

＜正＞ Aim To study the immunotherapeutic effects of ginsenoside Ro in multiple low dose streptozotocin （MLD-STZ） induced diabetic mice. Methods The diabetic mice were administered either ginsenoside Ro（25,50,150mg/kg/day） or saline per os and sacrificed after 10 or 20 days of treatment. Blood glucose level and the number of pancreas islet beta cells were measured, the nonspecific proliferation and specific proliferation ability of splenocytes to ConA and Insulin respectively were tested using [3H] thymidine incorporation assay,the level of cytokine IENγ and IL - 4 secreted by splenocytes were determined by ELISA method,and the expression of peroxisome proliferate - activated receptor gamma （PPARγ） gene was characterized using semi - quantitative RT - PCR. Results In day 20 of treatment, in experimental groups （50,150mg/kg/day）,the level of blood glucose and IFNγ,and specific proliferation ability of splenocytes to Insulin,decreased significantly;The number of islet beta cells, the level of IL - 4 and PPARγmRNA,however, increased significantly. Conclusion Ginsenoside Ro showed immunomodulatory and antihyperglycemic effects by revising the imbalance of Th1 and Th2 in MLDSTZ induced diabetic mice.

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蒎酸二酰基双去氢枞基双噁二唑的合成与表征

林产化学与工业 2010年第5期30卷 61-64页

作者：陈丽岑波段文贵董淑求广西大学化学化工学院广西南宁530004

以歧化松香中提取的去氢枞酸为原料,经酰化得到去氢枞酸酰氯。以松节油的主要成分α-蒎烯为原料,经过蒎酮酸、蒎酸、蒎酸二乙酯得到蒎酸二酰肼。蒎酸二酰肼与去氢枞酸酰氯在相转移催化剂的作用下反应,得到N,N′-二去氢枞基取代蒎酸二酰... 详细信息

以歧化松香中提取的去氢枞酸为原料,经酰化得到去氢枞酸酰氯。以松节油的主要成分α-蒎烯为原料,经过蒎酮酸、蒎酸、蒎酸二乙酯得到蒎酸二酰肼。蒎酸二酰肼与去氢枞酸酰氯在相转移催化剂的作用下反应,得到N,N′-二去氢枞基取代蒎酸二酰肼,再经三氯氧磷脱水环合,得到蒎酸二酰基双去氢枞基双噁二唑。初步探讨合成条件,通过元素分析、IR、MS、1H NMR和13C NMR对所合成的化合物进行了结构表征。

关键词：去氢枞酸 α-蒎烯双噁二唑

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黄芪多糖对1型糖尿病小鼠的免疫调节作用

中西医结合学报 2008年第2期6卷 166-170页

作者：李如江邱曙东陈红霞王丽蓉西安交通大学医学院生殖中心陕西西安710061 军事医学科学院毒物药物研究所北京100850

目的:探讨黄芪多糖(Astragalus polysaccharide,APS)对1型糖尿病的免疫调节作用。方法:以低剂量链脲菌素(streptozotocin,STZ)多次腹腔注射,建立1型糖尿病小鼠模型,每天分别用100、200、400mg/kg APS或1ml生理盐水腹腔注射,15d、30d时... 详细信息

目的:探讨黄芪多糖(Astragalus polysaccharide,APS)对1型糖尿病的免疫调节作用。方法:以低剂量链脲菌素(streptozotocin,STZ)多次腹腔注射,建立1型糖尿病小鼠模型,每天分别用100、200、400mg/kg APS或1ml生理盐水腹腔注射,15d、30d时将小鼠处死。以形态学方法观察胰岛炎症,以放射免疫法检测血胰岛素自身抗体,以放射性同位素氚标记的嘧啶核苷([3H]thymidine incorporation,3H-TdR)掺入法研究脾细胞对刀豆蛋白A(concanavalin A,Con A)的增殖反应,以酶联免疫吸附(enzymelinkedi mmunosorbent assay,ELISA)法检测脾细胞白细胞介素-4(interleukin-4,IL-4)和干扰素-γ(inter-feron-γ,IFN-γ)的分泌,以蛋白免疫印迹(Western-blot)法检验脾过氧化物酶体增殖物激活受体γ(peroxi-some proliferator-activated receptorγ,PPARγ)的表达。结果:与对照组比较,APS处理组胰岛炎症减轻,血胰岛素自身抗体水平下降,脾细胞对Con A的增殖能力减弱,Th1/Th2分泌因子比值下调,脾PPARγ水平提高,所有这些均与APS的剂量和使用持续时间密切相关。结论:APS可通过调节体液免疫和细胞免疫治疗小鼠1型糖尿病。

关键词：黄芪多糖糖尿病,1型免疫调节剂小鼠

Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

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Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi Qi Xueying Chen Bowen Liu Xuliang Lin Xueqing qiu Yanlin Qin Guangdong Provincial Key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

Fixed-point Theorems in Fuzzy Metric Spaces

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Fixed-point Theorems in Fuzzy Metric Spaces

Potassium and calcium channels in different nerve cells act as therapeutic targets in neurological disorders

第六届中国不确定系统年会

作者： You-li Chen~1 Zhi-wen Mo~1 Lan shu~2 dong qiu~2 1.College of Mathematics and Software Science,Sichuan Normal University,Chengdu,610066 2.School of Applied Mathematics,University of Electronic Science and Technology,Chengdu,610054

＜正＞In the paper,we give several fixed point theorems for fuzzy mappings on complete fuzzy metric spaces in the sense of George and Veeramani.

关键词： Fuzzy set Fuzzy metric space Fixed point

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Neural Regeneration Research 2025年第5期20卷 1258-1276页

作者： Qing qiu Mengting Yang Danfeng Gong Haiying Liang Tingting Chen Department of Pharmacology School of PharmacyNantong UniversityNantongJiangsu ProvinceChina Jiangsu Province Key Laboratory of Inflammation and Molecular Drug Target NantongJiangsu ProvinceChina Department of Pharmacy Longyan First Affiliated Hospital of Fujian Medical UniversityLongyanFujian ProvinceChina

The central nervous system, information integration center of the body, is mainly composed of neurons and glial cells. The neuron is one of the most basic and important structural and functional units of the central nervous system, with sensory stimulation and excitation conduction functions. Astrocytes and microglia belong to the glial cell family, which is the main source of cytokines and represents the main defense system of the central nervous system. Nerve cells undergo neurotransmission or gliotransmission, which regulates neuronal activity via the ion channels, receptors, or transporters expressed on nerve cell membranes. Ion channels, composed of large transmembrane proteins, play crucial roles in maintaining nerve cell homeostasis. These channels are also important for control of the membrane potential and in the secretion of neurotransmitters. A variety of cellular functions and life activities, including functional regulation of the central nervous system, the generation and conduction of nerve excitation, the occurrence of receptor potential, heart pulsation, smooth muscle peristalsis, skeletal muscle contraction, and hormone secretion, are closely related to ion channels associated with passive transmembrane transport. Two types of ion channels in the central nervous system, potassium channels and calcium channels, are closely related to various neurological disorders, including Alzheimer's disease, Parkinson's disease, and epilepsy. Accordingly, various drugs that can affect these ion channels have been explored deeply to provide new directions for the treatment of these neurological disorders. In this review, we focus on the functions of potassium and calcium ion channels in different nerve cells and their involvement in neurological disorders such as Parkinson's disease, Alzheimer's disease, depression, epilepsy, autism, and rare disorders. We also describe several clinical drugs that target potassium or calcium channels in nerve cells and could be u

关键词： astrocytes calcium channels central nervous system extracellular ion concentration microglia neurological disorders neurons potassium channels