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Fluorogenic sensing of amorphous aggregates,amyloid fibers,and chaperone activity via a near-infrared aggregation-induced emission-active probe

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Aggregate 2024年第1期5卷 309-320页

作者： Wei He Yuanyuan Yang Yuhui Qian Zhuoyi Chen Yongxin Zheng Wenping Zhao Chenxu Yan Zhiqian Guo shu quan State Key Laboratory of Bioreactor Engineering Shanghai Collaborative Innovation Center for Biomanufacturing(SCICB)East China University of Science and TechnologyShanghaiChina Key Laboratory for Advanced Materials and Joint International Research Laboratory of Precision Chemistry and Molecular Engineering Shanghai Key Laboratory of Functional Materials ChemistryFrontiers Science Center for Materiobiology and Dynamic ChemistryInstitute of Fine ChemicalsSchool of Chemistry and Molecular EngineeringEast China University of Science and TechnologyShanghaiChina Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism East China University of Science and TechnologyShanghaiChina

The presence of protein aggregates in numerous human diseases underscores the significance of detecting these aggregates to comprehend disease mechanisms and develop novel therapeutic approaches for combating these *** the development of various biosensors and fluorescent probes that selectively target amyloid fibers or amorphous aggregates,there is still a lack of tools capable of simultaneously detecting both types of ***,we demonstrate the quantitative discernment of amorphous aggregates by QM-FN-SO3,an aggregationinduced emission(AIE)probe initially designed for detecting amyloid *** probe easily penetrates the membranes of the widely-used prokaryotic model organism Escherichia coli,enabling the visualization of both amorphous aggregates and amyloid fibers through near-infrared ***,the probe exhibits sensitivity in distinguishing the varying aggregation propensities of proteins,regardless of whether they form amorphous aggregates or amyloid fibers in *** properties contribute to the successful application of the QM-FN-SO3 probe in the subsequent investigation of the antiaggregation activities of two outer membrane protein(OMP)chaperones,both in vitro and in their physiological ***,our work introduces a near-infrared fluorescent chemical probe that can quantitatively detect amyloid fibers and amorphous aggregates with high sensitivity in vitro and in ***,it demonstrates the applicability of the probe in chaperone biology and its potential as a high-throughput screening tool for protein aggregation inhibitors and folding factors.

关键词： aggregation-induced emission fluorescence molecular chaperone protein aggregation

Jianpi Gushen Huayu decoction ameliorated diabetic nephropathy through modulating metabolites in kidney,and inhibiting TLR4/NF-κB/NLRP3 and JNK/P38 pathways

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World Journal of Diabetes 2024年第3期15卷 502-518页

作者： Zi-Ang Ma Li-Xin Wang Hui Zhang Han-Zhou Li Li Dong Qing-Hai Wang Yuan-Song Wang Bao-ChaoPan shu-Fang Zhang Huan-Tian Cui shu-quan Lv Graduate School Hebei University of Chinese MedicineShijiazhuang 050000Hebei ProvinceChina Department of Endocrinology Cangzhou Hospital of Integrated Traditional Chinese Medicine and Western Medicine of Hebei Province Affiliated to Hebei University of Chinese MedicineCangzhou 061000Hebei ProvinceChina School of Integrative Medicine Tianjin University of Traditional Chinese MedicineTianjin 300000China The First School of Clinical Medicine Yunnan University of Traditional Chinese MedicineKunming 065000Yunnan ProvinceChina Department of Endocrinology Hebei Cangzhou Hospital of Integrative MedicineCangzhou 061000Hebei ProvinceChina

BACKGROUND Jianpi Gushen Huayu Decoction(JPGS)has been used to clinically treat diabetic nephropathy(DN)for many ***,the protective mechanism of JPGS in treating DN remains *** To evaluate the therapeutic effects and the possible mechanism of JPGS on *** We first evaluated the therapeutic potential of JPGS on a DN mouse *** then investigated the effect of JPGS on the renal metabolite levels of DN mice using non-targeted ***,we examined the effects of JPGS on c-Jun N-terminal kinase(JNK)/P38-mediated apoptosis and the inflammatory responses mediated by toll-like receptor 4(TLR4)/nuclear factor-kappa B(NF-κB)/NOD-like receptor family pyrin domain containing 3(NLRP3).RESULTS The ameliorative effects of JPGS on DN mice included the alleviation of renal injury and the control of inflammation and oxidative *** metabolomic analysis revealed that JPGS altered the metabolites of the kidneys in DN mice.A total of 51 differential metabolites were *** analysis results indicated that nine pathways significantly changed between the control and model groups,while six pathways significantly altered between the model and JPGS *** related to cysteine and methionine metabolism;alanine,tryptophan metabolism;aspartate and glutamate metabolism;and riboflavin metabolism were identified as the key pathways through which JPGS affects *** experimental validation showed that JPGS treatment reduced the expression of TLR4/NF-κB/NLRP3 pathways and JNK/P38 pathway-mediated apoptosis related *** JPGS could markedly treat mice with streptozotocin(STZ)-induced DN,which is possibly related to the regulation of several metabolic pathways found in ***,JPGS could improve kidney inflammatory responses and ameliorate kidney injuries in DN mice via the TLR4/NF-κB/NLRP3 pathway and inhibit JNK/P38 pathwaymediated apoptosis in DN mice.

关键词： Diabetic nephropathy Jianpi Gushen Huayu Decoction Oxidative stress Inflammation Untargeted metabolomics Toll-like receptor 4/nuclear factor-kappa B/NOD-like receptor family pyrin domain containing 3 pathway c-Jun N-terminal kinase/P38-mediated apoptosis

Stable and reversible zinc metal anode with fluorinated graphite nanosheets surface coating

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Transactions of Nonferrous Metals Society of China 2024年第10期34卷 3358-3371页

作者： Hong CHANG Zhen-ya LUO Xue-ru SHI Xin-xin CAO shu-quan LIANG Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan Province School of Materials Science and EngineeringCentral South UniversityChangsha 410083China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105China

A highly stable zinc metal anode modified with a fluorinated graphite nanosheets(FGNSs)coating was *** porous structure of the coating layer effectively hinders lateral mass transfer of Zn ions and suppresses dendrite ***,the high electronegativity exhibited by fluorine atoms creates an almost superhydrophobic solid-liquid interface,thereby reducing the interaction between solvent water and the zinc ***,this leads to a significant inhibition of hydrogen evolution corrosion and other side *** modified anode demonstrates exceptional cycling stability,as symmetric cells exhibit sustained cycling for over 1400 h at a current density of 5 mA/cm^(2).Moreover,the full cells with NH_(4)V_(4)O_(10)cathode exhibit an impressive capacity retention rate of 92.2%after undergoing 1000 cycles.

关键词： fluorinated graphite hydrophobic coating anti-corrosion dendrite suppression zinc metal anode

Copper-based materials in anode electrode of aqueous zinc metal batteries

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cMat 2024年第2期1卷 91-109页

作者： Hai-Long Li Zhe-Xuan Liu Yan Tang shu-quan Liang Guo-Zhao Fang School of Materials Science and Engineering Central South UniversityChangshaChina Key Laboratory of Electronic Packaging and Advanced Functional Materials of Hunan Province Central South UniversityChangshaChina

Aqueous zinc metal batteries have attracted much attention in the field of large-scale energy storage due to their abundant resources,high safety,low cost,and high theoretical energy ***,Zn anodes suffer from serious problems such as dendrite growth,hydrogen evolution reaction,corrosion,and ***-based materials have a wide range of applications in Zn anodes due to their excellent ***,relevant review on Cu-based materials in anode electrode is still *** review focuses on the progress,issues,and optimization strategies of Cu-based materials in Zn *** application of Cu collectors in Zn anodes and the corresponding modifications are also ***,insights and future directions related to Cu-based materials for modified Zn anode and Cu collectors are presented to provide scientific guidance for future research.

关键词： collector copper-based materials zinc anode zinc metal batteries

Exploring the molecular mechanism of action of curcumin for the treatment of diabetic retinopathy,using network pharmacology,molecular docking,and molecular dynamics simulation

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Integrative Medicine Discovery 2024年第8期8卷 1-10页

作者： Yuan-Yuan Gan Yan-Mei Xu quan shu Qi-Zhi Huang Tian-Long Zhou Ju-Fang Liu Wei Yu School of Pharmacy Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Second Affiliated Hospital Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Department of Medicine Xianning Medical CollegeHubei University of Science and TechnologyXianning 437100China Department of Obstetrics and Gynecology Xianning Central HospitalThe First Affiliated Hospital of Hubei University of Science and TechnologyXianning 437000China.

Background:Based on network pharmacology and molecular docking,the present study investigated the mechanism of curcumin(CUR)in diabetic retinopathy ***:Based on the DisGeNET,Swiss TargetPrediction,GeneCards,Online Mendelian Inheritance in Man,Gene Expression Omnibus,and Comparative Toxicogenomics Database,the intersection core targets of CUR and diabetic retinopathy were *** intersection target was imported into the STRING database to obtain the protein-protein interaction *** to the Database for Annotation,Visualization and Integrated Discovery database,the intersected targets were enriched in Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes *** Cytoscape 3.9.1 is used to make the drug-target-disease-pathway *** mechanism of CUR and diabetic retinopathy was further verified by molecular docking and molecular dynamics ***:There were 203 intersecting targets of CUR and diabetic retinopathy identified.1320 GO entries were enriched for GO functions,which were primarily involved in the composition of cells such as identical protein binding,protein binding,enzyme binding,*** was found that 175 pathways were enriched using Kyoto Encyclopedia of Genes and Genomes pathway enrichment methods,which were mainly included in the lipid and atherosclerosis,AGE-RAGE signaling pathway in diabetic complications,pathways in cancer,*** the molecular docking analysis,CUR was found to have a good ability to bind to the core targets of albumin,IL-1B,and *** binding of albumin to CUR was further verified by molecular dynamics ***:As a result of this study,CUR may exert a role in the treatment of diabetic retinopathy through multi-target and multi-pathway regulation,which indicates a possible direction of future research.

关键词： curcumin diabetic retinopathy network pharmacology molecular docking molecular dynamics simulation

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促进汉江湖北段流域经济高质量发展策略

交通企业管理 2024年第2期39卷 7-9页

作者：全姝陈博仡中交第二航务工程勘察设计院有限公司

汉江是长江水系高等级航道网的主要支流,是湖北省综合运输体系的重要组成部分,在湖北区域发展格局中具有重要地位。2018年汉江生态经济带上升为国家战略后,汉江流域经济发展迎来重大机遇。总体来看,汉江湖北段经济基础良好、工业基础雄... 详细信息

汉江是长江水系高等级航道网的主要支流,是湖北省综合运输体系的重要组成部分,在湖北区域发展格局中具有重要地位。2018年汉江生态经济带上升为国家战略后,汉江流域经济发展迎来重大机遇。总体来看,汉江湖北段经济基础良好、工业基础雄厚,流域经济发展整体向好,但也存在产业链上下游分工协作不足、港产融合发展有待提高等问题。

关键词：汉江生态经济带流域经济港产融合产业链分工协作

儿童急性白血病化疗后合并真菌血流感染的临床特征及预后分析

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中国当代儿科杂志 2024年第10期26卷 1086-1092页

作者：翁开枝吴椿萍庄树铨黄淑贤王晓芳郑湧智福建医科大学附属漳州市医院儿童血液风湿肾科福建漳州363000 福建医科大学附属协和医院小儿血液科/福建省血液病研究所/福建省血液病学重点实验室福建福州350001 福建医科大学附属泉州第一医院儿科福建泉州362000

目的探讨儿童急性白血病(acute leukemia,AL)化疗后合并真菌血流感染(bloodstream infection,BSI)的临床特征及预后。方法回顾性选择2015年1月—2023年12月在福建3家医院行AL化疗后合并真菌BSI的23例患儿作为研究对象,对其临床特征及预... 详细信息

目的探讨儿童急性白血病(acute leukemia,AL)化疗后合并真菌血流感染(bloodstream infection,BSI)的临床特征及预后。方法回顾性选择2015年1月—2023年12月在福建3家医院行AL化疗后合并真菌BSI的23例患儿作为研究对象,对其临床特征及预后进行分析。结果所有AL患儿化疗后合并真菌BSI的发生率为1.38%(23/1668)。在发生真菌BSI时,87%(20/23)患儿中性粒细胞缺乏已持续超过1周;临床上均有发热表现,22%(5/23)伴脓毒性休克表现。23例(100%)均有C反应蛋白及降钙素原水平明显升高。外周血培养共检出23株真菌,其中以热带念珠菌(52%,12/23)最为常见。卡泊芬净或米卡芬净联合两性霉素B脂质体治疗有效率较高(75%,12/16),抗真菌疗程中位数为3.0个月。总体病死率为35%(8/23),归因死亡率为22%(5/23)。结论儿童AL化疗后合并真菌BSI多发生于持续中性粒细胞缺乏,临床表现缺乏特异性,病原菌以热带念珠菌最为常见,抗真菌疗程长,病死率高。

关键词：侵袭性真菌病血流感染急性白血病抗真菌治疗预后儿童

大数据背景下船员培训驾驶舱平台设计与实现——以浙江国际海运职业技术学院船员培训为例

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武汉船舶职业技术学院学报 2024年第3期23卷 104-110页

作者：舒伟权朱俊彦浙江国际海运职业技术学院浙江舟山316021

以大数据时代为背景,以浙江国际海运职业技术学院船员培训系统为例,设计并实现了一套基于大数据的船员培训驾驶舱平台。通过引入Hadoop、Hive、Spark等大数据技术实现了船员培训数据的采集、分析和可视化展示。该平台的设计目标在于提... 详细信息

以大数据时代为背景,以浙江国际海运职业技术学院船员培训系统为例,设计并实现了一套基于大数据的船员培训驾驶舱平台。通过引入Hadoop、Hive、Spark等大数据技术实现了船员培训数据的采集、分析和可视化展示。该平台的设计目标在于提升船员培训效果,通过大数据存储分析技术对培训项目、人次、计划等数据进行采集和分析,形成主题评估及数据对比,为管理层决策提供支撑。详细介绍了平台的总体架构、环境准备、数据导入、清洗、处理、持久化和可视化等关键步骤,强调了数据处理的全流程。

关键词：大数据技术船员培训平台设计数据可视化

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盐穴储气库多步造腔流场浓度场规律

科学技术与工程 2024年第24期24卷 10268-10279页

作者：王进超汪志明曾泉树王俊中国石油大学(北京)石油工程学院北京102249 油气资源与探测国家重点实验室(中国石油大学(北京)) 北京102249

盐穴储库水溶造腔过程中流场特征和浓度场分布会很大程度影响腔体的扩展和腔体最终形态,而流场特征和浓度分布无法通过技术手段进行监测。为研究盐穴水溶多阶段造腔过程中流场特征、浓度场分布及它们对腔体扩展的影响规律,基于建立的盐... 详细信息

盐穴储库水溶造腔过程中流场特征和浓度场分布会很大程度影响腔体的扩展和腔体最终形态,而流场特征和浓度分布无法通过技术手段进行监测。为研究盐穴水溶多阶段造腔过程中流场特征、浓度场分布及它们对腔体扩展的影响规律,基于建立的盐穴储库多场耦合流动模型进行了相关的数值模拟计算,并与金坛两口储库井进行数据对比。模拟结果与现场造腔数据相一致,验证了模型的准确性。基于模拟结果和数据,分析了水溶多阶段造腔各阶段的流场特征、浓度场分布规律,并分析流场和浓度场的分布如何影响腔体的扩展。结果表明:正循环造腔会形成一个涡流,主要起冲蚀作用,一定程度抑制溶蚀作用;反循环会形成两个涡流,下部的促进卤水排出,上部促进溶蚀作用;强制对流会引起涡流,涡流强度随腔体扩大逐渐减弱,涡流使腔内流体流动,促进了对流扩散过程,从而影响浓度场的分布,最终影响到腔体扩展,通过调整正反循环方式、管柱的位置、油垫位置及注入量可以控制腔体的形态扩展;造腔前期主要受到冲蚀作用,造腔效率高,腔体表面积小使得造腔速率较慢,腔体主要向中下部扩展;后期随着腔体扩大,主要以溶蚀作用为主,造腔速率高,腔体主要向中上部扩展。研究结果和规律对现场造腔工艺设计提供一定的理论基础,对提高现场造腔效率、优化造腔工艺参数具有指导意义。

关键词：盐穴储气库多阶段造腔流动特征浓度场腔体形态