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Pattern-based rapid and robust profiling of gut microbiota and its metabolism with a single fluorescent probe

Science China Chemistry 2025年第2期68卷 781-788页

作者： Hongli Tan Meizi Zeng Cong Fang Xiaohua Zhu Feng Liu Ying Long Ruijie Deng Peng Yin Meiling Liu Haitao Li Youyu Zhang shouzhuo yao Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research(Ministry of Education) College of Chemistry and Chemical EngineeringInstitute of Interdisciplinary StudiesHunan Normal UniversityChangsha 410081China Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine Central South UniversityChangsha 410013China College of Biomass Science and Engineering Healthy Food Evaluation Research CenterSichuan UniversityChengdu 610064China

The gut microbiota and the associated metabolism play a pivotal role in maintaining human health,yet their assessment is challenging due to their inherent diversity,variety,and ***,we developed a multichannel sensor array based on a single fluorescent probe,allowing for rapid and robust profiling gut microbiota and their key metabolites including cysteine(Cys),glutathione(GSH),and homocysteine(Hcy).The assay leverages the single fluorescent probe that is with multiple binding sites and the cross-reactive sensing principle to specifically recognize different biological *** analyzing the pattern of biological thiols,the assay is capable of rapidly identifying six gut-derived bacteria including probiotics,neutral,and pathogenic strains based on fluorescent fingerprints within 5 min,and also discriminating bacteria and their mixtures with different *** the assay that enables the simultaneous measurement of multiple gut-derived bacteria and their metabolites,the designed array achieved an accuracy of 0.99 when discriminating colorectal cancer(CRC)patient feces samples from healthy ***,the as-prepared sensor array can also be used to identify various stages of *** simplicity,rapidness,and cost-effectiveness of our approach render it a robust platform for the analysis of gut microbiota.

关键词： gut microbiota biological thiols sensor array fluorescent probe

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Separation of acidic and basic proteins by capillary electrophoresis using gemini surfactants and gemini-capped nanoparticles as buffer additives

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Science China Chemistry 2009年第10期52卷 1666-1676页

作者： LIU Qian LI YanQing YANG YanMin yao shouzhuo State Key Lab of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

This paper demonstrated simultaneous separation of acidic and basic proteins using cationic gemini surfactants as buffer additives in capillary electrophoresis. We showed that even at a low concentration (0.1 mmol·L-1) of alkanediyl-α,ω-bis(dimethyloctadecylammonium bromide) (18-s-18), the wall adsorption of both acidic and basic proteins could be effectively suppressed under acidic conditions. Smaller micelle size (e.g., s=5-8) is more effective for the separation of acidic proteins than larger micelle size (e.g., s10). Varying the spacer length of gemini surfactants can influence the electrophoretic mobility and selectivity of proteins to achieve the desired separation. Under the optimized conditions, RSDs of the migration time were less than 0.8% and 2.2% for run-to-run and day-to-day assays, respectively, and protein recoveries ranged from 79% to 100.4%. Furthermore, we also investigated the use of gemini surfactant-capped gold nanoparticles (gemini@AuNPs) as buffer additives in protein separation. Introduction of AuNPs into the buffer shortened the analysis time and slightly improved the separation efficiencies. Finally, we presented the applications of this method in the analysis of bio-logical samples, including plasma, red blood cells and egg white.

关键词： gemini surfactant protein separation capillary electrophoresis gold nanoparticle

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Pattern-based rapid and robust profiling of gut microbiota and its metabolism with a single fluorescent probe

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Science China Chemistry 2025年第2期 781-788页

作者： Hongli Tan Meizi Zeng Cong Fang Xiaohua Zhu Feng Liu Ying Long Ruijie Deng Peng Yin Meiling Liu Haitao Li Youyu Zhang shouzhuo yao Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education) College of Chemistry and Chemical EngineeringInstitute of Interdisciplinary StudiesHunan Normal University Hunan Cancer Hospital/The Affiliated Cancer Hospital of Xiangya School of Medicine Central South University College of Biomass Science and Engineering Healthy Food Evaluation Research CenterSichuan University

The gut microbiota and the associated metabolism play a pivotal role in maintaining human health, yet their assessment is challenging due to their inherent diversity, variety, and complexity. Herein, we developed a multichannel sensor array based on a single fluorescent probe, allowing for rapid and robust profiling gut microbiota and their key metabolites including cysteine(Cys), glutathione(GSH), and homocysteine(Hcy). The assay leverages the single fluorescent probe that is with multiple binding sites and the cross-reactive sensing principle to specifically recognize different biological thiols. By analyzing the pattern of biological thiols, the assay is capable of rapidly identifying six gut-derived bacteria including probiotics, neutral, and pathogenic strains based on fluorescent fingerprints within 5 min, and also discriminating bacteria and their mixtures with different composition. Using the assay that enables the simultaneous measurement of multiple gut-derived bacteria and their metabolites, the designed array achieved an accuracy of 0.99 when discriminating colorectal cancer(CRC) patient feces samples from healthy individuals. Remarkably, the as-prepared sensor array can also be used to identify various stages of CRC. The simplicity, rapidness, and cost-effectiveness of our approach render it a robust platform for the analysis of gut microbiota.

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The direct electrochemistry of glucose oxidase based on the synergic effect of amino acid ionic liquid and carbon nanotubes

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Science China Chemistry 2009年第11期52卷 1991-1998,2033-2034页

作者： WANG MengDong DENG ChunYan NIE Zhou XU XiaHong yao shouzhuo State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China

Amino acid ionic liquids(AAILs) have attracted much attention due to their special chemical and physical properties,especially their outstanding biocompatibility and truly green *** this work,a novel electrochemical biosensing platform based on AAILs/carbon nanotubes(CNTs) composite was *** were used as a novel solvent for glucose oxidase(GOD) and the GOD-AAILs/CNTs/GC electrode was conveniently prepared by immersing the carbon nanotubes(CNTs) modified glassy carbon(GC) electrode into AAILs containing *** direct electrochemistry of GOD on the GOD-AAILs/CNTs/GC electrode has been investigated and a pair of reversible peaks was obtained by cyclic *** immobilized glucose oxidase could retain bioactivity and catalyze the reduction of dissolved *** to the synergic effect of AAILs and CNTs,the GOD-AAILs/CNTs/GC electrode shows excellent electrocatalytic activity towards glucose with a linear range from 0.05 to 0.8 mM and a detection limit of 5.5 μM(S/N = 3).Furthermore,the biosensor exhibits good stability and ability to exclude the interference of commonly coexisting uric and ascorbic ***,AAILs/CNTs composite can be a good candidate biocompatible material for the direct electrochemistry of the redox-active enzyme and the construction of third-generation enzyme sensors.

关键词： amino acid ionic liquids(AAILs) carbon nanotubes(CNTs) glucose oxidase(GOD) direct electrochemistry glucose sensor

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（4-Ferrocenylethyne） phenylamine on Graphene as the Signal Amplificator to Determinate Dopamine and Acetaminophen Simultaneously

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Chinese Journal of Chemistry 2013年第6期31卷 845-854页

作者： Linping Wang Meiling Liu Yue Meng Haitao Li Youyu Zhang shouzhuo yao Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research (Ministry of Education) College of Chemistry and Chemical Engineering Hunan Normal University Changsha Hunan 410081 China

A novel （4-ferrocenylethyne） phenylamine functionalized graphene sheets （FEPA-GR）, coupling with chitosan （CS） were used as a signal amplification platform for simultaneous and sensitive determination of dopamine （DA） and acetaminophen （AC）. In this work, FEPA used as electron transfer mediator can be immobilized on GR surface via strong π-π stacking interaction between the conjugate chain of FEPA and GR, which effectively prevents FEPA electron mediator leaking from the electrode surface and amplified the signal. Transmission electron microscopy, FT-IR spectroscopy, UV-vis spectroscopy and electrochemical experiments results are all demonstrated the strong π-π stacking interaction between FEPA and GR. The resulted biosensor exhibited a fast response, remarkable electrocatalytic activity, perfect anti-interference ability and good stability for simultaneous detection of DA and AC. Under the optimum conditions, the oxidation peak currents of DA and AC were linearly correlated to their concentrations in the range of 2.0-135.0 μmol·L-1 and 0.3--80.0μmol·L 1, respectively. The lower detection limits for DA and AC were 0.30 and 0.05 μmol·L-1, respectively. The feasibility of the proposed method was validated by successfully applied to the simultaneous determination of DA and AC in serum samples with the standard addition method.

关键词： simultaneous electrochemical detection (4-ferrocenylethyne) phenylamine π-π stacking

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A piezoelectric spectro- electrochemical study on the structural interconversion for poly(o-phenylenediamine) between its ladder structure with phenazine units and polyaniline-like chains

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Chinese Science Bulletin 2005年第15期50卷 1598-1604页

作者： TU Xinman XIE Qingji WANG Meiling ZHANG Youyu yao shouzhuo College of Chemistry and Chemical Engineering Key Laboratory of Chemical Biology and Traditional Chinese Medicine Research Ministryof Education Hunan Normal University Changsha 410081 China

The combination of reflectance UV-Vis spec- troelectrochemistry with electrochemical quartz crystal microbalance (EQCM) and separate reflectance FTIR char- acterization were used to investigate the structural intercon- version for poly(o-phenylenediamine) (PoPD) between its ladder structure with phenazine units and polyaniline-like linear chains. The poly(o-phenylenediamine) films potentio- statically (0.8 V vs. SCE) grew on Au electrodes from 0.20 mol·L?1 H2SO4 (PoPD1) or 0.40 mol·L?1 NaOH (PoPD2) aque- ous solution containing 0.20 mol·L?1 Na2SO4 + 0.10 mol·L?1 o-phenylenediamine. By considering the mass of deposited PoPD2 film obtained from the EQCM data and the charge consumed under the current peak at ca. 0.6 V vs. SCE for oxidation of -NH2 groups in as-prepared PoPD2 during po- tential cycling in 0.10 mol·L-1 aqueous H2SO4, the molar per- centage of the polyaniline-like chains was estimated to be 19% (relative to total phenylenediamine units), being in agreement with the result obtained from a formaldehyde- combination experiment through the aminocarbonyl reaction. After 40-cycle potential sweeps between 0.2 and 0.8 V vs. SCE the polyaniline-like chains in PoPD2 could be com- pletely converted via intramolecular cyclization into the lad- der structure with phenazine units. However, PoPD1 was found to be perfectly composed of the ladder structure with phenazine units, and after 40-cycle potential sweeps between ?0.4 and 0.1 V vs. SCE only 2.5% in molar percentage of PoPD’s ladder structure could be converted into polyani line-like chains, suggesting that the ladder structure with phenazine units is thermodynamically more stable due to its possessing higher conjugation.

关键词：压电效应光谱电子学阶梯结构聚乙烯结构互换

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Assembly of layer-by-layer films of superoxide dismutase and gold nanorods:A third generation biosensor for superoxide anion

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Science China Chemistry 2011年第8期54卷 1284-1291页

作者： WANG MengDong HAN YiTao LIU XingXing NIE Zhou DENG ChunYan GUO ManLi yao shouzhuo State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University Changsha 410082 China College of Chemistry and Chemical Engineering Central South University Changsha 410083 China

Based on the layer-by-layer self-assembly of positively charged cetyltrimethylammonium bromide （CTAB） wrapped gold na- norods （AuNRs） and negatively charged superoxide dismutase （SOD） from their aqueous solutions on cysteine modified gold electrode （Cys/Au）, a third generation electrochemical biosensor （（SOD/AuNRs）2/Cys/Au） for superoxide anion （02＂-） was developed. The two layers assembly of SOD/AuNRs can significantly enhance the direct electron transfer between SOD and the electrode. The functional enzymatic activities of the SOD offer an electrochemical approach to the determination of 02＂-. In the reductive regions, the proposed sensor exhibits excellent analytical performances, such as wide linear range （200 nM to 0.2 mM O2-）, low detection limit （100 nM O2-）, high sensitivity （22.11 nA cm-2 μM-1）, short response time （less than 5 s）, good stability and reproducibility, while no obvious interferences are caused by commonly met interfering species including hydrogen peroxide （H202）, uric acid （UA） and ascorbic acid （AA）.

关键词： superoxide dismutase (SOD) gold nanorods (AuNRs) layer-by-layer self-assembly direct electrochemistry superoxideanion sensor

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Biocompatibility and in vitro antineoplastic drug-loaded trial of titania nanotubes prepared by anodic oxidation of a pure titanium

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Science China Chemistry 2009年第12期52卷 2161-2165页

作者： XIAO XiLin YANG LiXia GUO ManLi PAN ChunFeng CAI QingYun yao shouzhuo State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityChangsha 410082China College of Chemistry and Chemical Engineering University of South ChinaHengyang 421001China

TiO2 nanotube (NT) arrays have been prepared by anodic oxidation of a Ti sheet,and carbon-deposited TiO2 NT arrays have been prepared by annealing TiO2 NT arrays in carbon atmosphere. The biocompatibility of the as-prepared NT arrays was investigated by observing the growth of osteosarcoma (MG-63) cells on the NT arrays. The application of the TiO2 NT arrays as a drug delivery vehicle was investigated. Both the TiO2 NTs and the carbon-modified TiO2 NTs have good biocompatibility supporting the normal growth and adhesion of MG-63 cells with no need of extracellular matrix protein coating. The one end-opened TiO2 NTs can be easily filled with drugs,working as an efficient drug delivery vehicle.

关键词： drug delivery biocompatibility titania nanotubes cell adhesion cisplatin

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Sensitive detection of DNA methyltransferase activity based on supercharged fluorescent protein and template-free DNA polymerization

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Science China Chemistry 2016年第7期59卷 809-815页

作者： Daiqi Li Guoyan Lu Chunyang Lei Zhen Wang Lijun Li Zhou Nie Yan Huang shouzhuo yao State Key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Hunan University

DNA methylation, catalyzed by DNA methyltransferases(MTases), is a key component of genetic regulation, and DNA MTases have been regarded as potential targets in anticancer therapy. Herein, based on our previously developed DNA-mediated supercharged green fluorescent protein(Sc GFP)/graphene oxide(GO) interaction, coupled with methylation-initiated template-free DNA polymerization, we propose a novel fluorescence assay strategy for sensitive detection of DNA MTase activity. A hairpin DNA with a methylation-sensitive site and an amino-modified 3′-terminal(DNA-1) was designed and worked as a starting molecule. In the presence of DNA MTase, methylation-sensitive restriction endonuclease, and terminal deoxynucleotidyl transferase(Td T), DNA-1 can be sequentially methylated, cleaved, and further elongated. The resulting long DNA fragments quickly bind with Sc GFP and form the Sc GFP/DNA nanocomplex. Such nanocomplex can effectively protect Sc GFP from being adsorbed and quenched by GO. Without the methylation-initiated DNA polymerization, the fluorescence of Sc GFP will be quenched by GO. Thus, the DNA MTase activity, which is proportional to the amount of DNA polymerization products, can be measured by reading the fluorescence of Sc GFP/GO. The method was successfully used to detect the activity of DNA adenine methylation(Dam) MTase with a wide linear range(0.1–100 U/m L) and a low detection limit of 0.1 U/m L. In addition, the method showed high selectivity and the potential to be applied in a complex sample. Furthermore, this study was successfully extended to evaluate the inhibition effect of 5-fluorouracil on Dam MTase activity and detect Td T activity.

关键词： DNA methyltransferase supercharged fluorescent protein graphene oxide DNA polymerization

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Electrochemical Sensor for Sensitive Determination of Ga（III） Ion Based on β-Cyclodextrin Incorporated Multi-walled Carbon Nanotubes and Imprinted Sol-gel Composite Film

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Chinese Journal of Chemistry 2012年第2期30卷 377-385页

作者： Hu,Yufang Zhang,Zhaohui Zhang,Huabin Luo,Lijuan Zhang,Minglei Yang,Xiao yao,shouzhuo College of Chemistry and Chemical Engineering Jishou UniversityJishouHunan 416000China State Key Laboratory of Chemo/Biosensing and Chemometrics ChangshaHunan 410082China

A novel sensor for detection of trace gallium ion [Ga（III）] was created by stepwise modification of a gold electrode with fl-cyclodextrin （β-CD）/multi-walled carbon nanotubes （MWCNTs） and an ion imprinted polymer （IIP）. The sensor surface morphology was characterized by scanning electron microscopy. The electrochemical performance of the imprinted sensor was investigated by cyclic voltammetry, differential pulse voltammetry and chronoamperometry. The sensor displayed excellent selectivity towards the target Ga（III） ion. Meanwhile, the introduced MWCNTs displayed noticeable catalytic activity, and fl：CD demonstrated significant enrichment capacity. A linear calibration curve was obtained covering the concentration range from 5.0 × 10 8 to 1.0 × 10-4 moloL-1, with a detection limit of 7.6× 10 9 mol·L-1. The proposed sensor was successfully applied to detect Ga（III） in real urine samples.

关键词： ion imprinted electrochemical sensor multi-walled carbon nanotubes β-cyclodextrin Ga(III)-8- hydroxyquinoline complex

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