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Transcriptome Analysis of Soybean in Response to Different Sulfur Concentrations

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Phyton-International Journal of Experimental Botany 2022年第6期91卷 1165-1182页

作者： Xiyue Wang Xiaomei Li Zihao Wu shoukun dong Northeast Agricultural University Harbin150030China Harbin Academy of Agricultural Science Harbin150029China

Sulfur is an indispensable nutrient for plant growth and development,and is important in the synthesis of sulfurcontaining amino *** several studies on the effects of some macronutrients,including nitrogen and phosphorus,have been conducted on the performance of several crops at the genomic level,studies on the effect of sulfur on crop performance are ***,this study aimed to examine the effect of different sulfur concentration on the transcriptome of ***,soybean yield parameters were also *** soybean varieties,DND252 and HN84,were exposed to low and high concentrations of sulfur,and differentially expressed genes(DEGs)were identified using transcriptome *** study results showed that the DEGs identified in the DND252 variety were involved in stimuli response,DNA binding and cell periphery under low sulfur ***,the DEGs identified under high sulfur concentration were involved in membrane and membrane ***,DEGs identified in the HN84 variety under low sulfur concentrations had similar functions as those identified in DND252 under high sulfur concentrations,indicating that HN84 was more sensitive to sulfur concentration changes than ***,under higher sulfur concentrations,the DEGs identified in HN84 were primarily involved in membrane and membrane parts,indicating that high sulfur can cause cell membrane ***,soybean grown using 2.0 mmol/L sulfur had the best *** findings of this study identified candidate genes for the breeding and development of sulfur-efficient soybean varieties.

关键词： Soybean sulfur transcriptome

Proteomics Analysis of Soybean Seedlings under Short-Term Water Deficit

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Phyton-International Journal of Experimental Botany 2022年第7期91卷 1381-1401页

作者： Xiyue Wang Zihao Wu Chao Yan Chunmei Ma shoukun dong College of Agriculture Northeast Agricultural UniversityHarbin150030China

Soybeans are one of the most important grain crops *** deficit,which seriously affects the yield and quality of soybeans,is the main abiotic stress factor in soybean *** a follow-up study,the droughttolerant soybean variant Heinong 44 was analyzed via proteome *** was exposed to water deficit for 0,8,and 24 h,and protein samples were extracted for detection of differentially expressed *** sequencing of leaf tissues under water stress yielded a total of 549 differentially expressed proteins:75 and 320 upregulated proteins as well as 70 and 84 downregulated proteins were obtained after 8 and 24 h of water deficit,*** Ontology analysis revealed that most of the differentially expressed proteins(DEPs)were involved in catalytic activity,molecular function,and metabolic processes,whereas some of them were involved in photosynthesis,carbon metabolism,and energy *** also identified some differentially expressed proteins that may be involved in the regulation of water deficit *** study provides a theoretical basis for the breeding of drought-resistant soybean varieties.

关键词： Soybean water deficit proteomics stress time

硫素水平对大豆叶片碳氮代谢关键酶活性的影响

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中国农学通报 2024年第12期40卷 113-118页

作者：王小竟李永平宋爽周琦王鑫董守坤东北农业大学农学院哈尔滨150030

为了探究不同的硫素水平对大豆苗期和花期叶片碳氮代谢关键酶活性的影响,以大豆品种‘来豆2号’和‘合丰55号’为试材,用浇灌营养液的方法测大豆苗期和花期叶片碳代谢关键酶活性的数据。‘来豆2号’和‘合丰55号’的GOGAT和SS酶活性在16... 详细信息

为了探究不同的硫素水平对大豆苗期和花期叶片碳氮代谢关键酶活性的影响,以大豆品种‘来豆2号’和‘合丰55号’为试材,用浇灌营养液的方法测大豆苗期和花期叶片碳代谢关键酶活性的数据。‘来豆2号’和‘合丰55号’的GOGAT和SS酶活性在160 mg/L处理下达最大值;‘来豆2号’GS酶活性在160 mg/L处理下酶活性峰值最强,‘合丰55号’苗期峰值是260 mg/L处理下达最大值,花期峰值是硫营养最低的20 mg/L处理下;‘来豆2号’SPS酶活性在40 mg/L处理下达最大值,而‘合丰55号’苗期峰值是硫营养较高的80 mg/L处理,花期峰值是硫素水平最高的260 mg/L处理。结果表明,谷氨酸合成酶(GOGAT)、谷氨酰胺合成酶(GS)、蔗糖磷酸合成酶(SPS)和蔗糖合成酶(SS)的开花期酶活性显著大于苗期,品种间呈相同规律。

关键词：硫素水平大豆碳氮代谢酶活性苗期花期

Effects of Drought Stress on Key Enzymes of Carbon Metabolism, Photosynthetic Characteristics and Agronomic Traits of Soybean at the Flowering Stage under Different Soil Substrates

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Phyton-International Journal of Experimental Botany 2022年第11期91卷 2475-2490页

作者： Shuang Song Xiaomei Li Xin Wang Qi Zhou Yongping Li Xiaojing Wang shoukun dong College of Agriculture Northeast Agricultural UniversityHarbin150030China Harbin Academy of Agricultural Sciences Harbin150030China

Soybean is an important legume food crop,and its seeds are rich in nutrients,providing humans and animals with edible oil and protein ***,soybean is sensitive to water requirements,and drought is an important factor limiting soybean yield and *** study used Heinong 84(drought resistant variety)and Hefeng 46(intermediate variety)as tested varieties planted in chernozem,albic,and black *** effects of drought stress on the activities of key enzymes in carbon metabolism and photosynthetic characteristics of soybean were studied during the flowering stage,most sensitive to water.(1)The activities of SS-1,6PGDH,and G6PDH enzymes in soybean leaves first increased and then decreased under drought *** enzyme activity was the highest under moderate drought stress and weakest in the blank group.(2)Drought stress increased Phi2,PhiNO,and Fm in soybean leaves and reached the highest value under severe drought;with the increase in drought stress,PhiNPQ and Fv/Fm of soybean leaves gradually decreased,reaching the lowest under severe drought.(3)With the increase in drought stress,F0 and Fs of soybean leaves showed a single peak curve,and the maximum was at moderate drought.(4)Correlation analysis showed that F0 was greatly affected by varieties and soil types;Fs,F0,and Fm soil varieties had a great influence,and chlorophyll fluorescence parameters were affected differently under drought stress with different drought degrees.(5)Drought stress changed the agronomic traits and yield of *** the increase of drought degree,plant height,node number of main stem,effective pod number,100-seed weight and total yield decreased continuously.(6)Drought stress affected the dry matter accumulation of *** the increase of drought degree,the dry matter accumulation gradually *** them,the leaf was most seriously affected by drought,and SD decreased by about 55%compared with *** the condition of black soil,the dry matter accumulation of

关键词： Soybean drought chlorophyll fluorescence parameters agronomic character key enzymes of carbon metabolism correlation analysis

Study on Accumulation Rule of Carbohydrates in Soybean(Glycine max(L.) Merrill)

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Journal of Northeast Agricultural University(English Edition) 2009年第1期16卷 1-4页

作者： SONG Baiquan LIU lijun dong shoukun ZU Wei SUN Congshu College of Agriculture Northeast Agricultural University Harbin 150030 China

Three soybean cultivars, Hefeng25 （HF25）, Suinongl4 （SN14） and Longxuanl （LX1） were used as materials and the accumulation features of carbohydrates were studied in leaves and stems. The results showed that the soluble sugar content in leaves and stems appeared the same trend of higher-lower-higher in three varieties. The changes of sucrose content in leaves appeared the same rule with that of soluble sugar, that is higher-lower-higher, while presented a rising trend in stems. The content of starch in leaves varied as a change of double apexes. The content of starch in stems appeared a change of single apex. In the same stage, there were some differences among carbohydrates. The content of soluble sugar and sucrose in SN14 was higher, and the starch content was lower.

关键词： soybean soluble sugar sucrose starch

Effects of straw retention and phosphorous fertilizer application on available phosphorous content in the soil solution during rice growth

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Effects of straw retention and phosphorous fertilizer applic...

干旱胁迫及复水对大豆抗坏血酸-谷胱甘肽循环的影响

中国农学会耕作制度分会2018年度学术年会

作者：闫超龚振平 Houqiang Zhan Shuangshuang Yan shoukun dong Chunmei Ma Qiulai Song College of Agriculture Northeast Agricultural University

[Research Objectives]Phosphorus(P) is an essential nutrient for proper rice growth, and available P in the soil solution is a direct source of P for rice uptake. [Methods] In this study, a field planting(experiment A: straw retention(SRT) treatment and straw removal(SRM) treatment) exposed to three years of continuous SRT and pot plantings(experiment B: five P levels;SRT and SRM treatments) with different concentrations of applied P fertilizer were used to study the effects of straw retention and P fertilizer application on the available P concentration in the soil solution during rice growth and on rice yield. [Results] During the 10-to 20-day period after transplanting, the available P concentration in the soil solution was high;the concentration decreased thereafter. The available P concentration in the soil solution in the SRT group decreased during rice growth, although straw retention did not alter the trend of available P concentration in the soil solution. [Conclusion] The application of P fertilizer increased the available P concentration in the soil solution, and the rice yield increased with increasing applications of P fertilizer. Straw retention reduced the rice yield at low P levels but increased the rice yield at high P levels.

关键词： rice straw retention phosphorous fertilizer soil solution phosphorus

来源： cnki会议评论

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东北农业大学学报 2018年第1期49卷 10-18页

作者：董守坤马玉玲李爽董娜刘丽君东北农业大学农学院哈尔滨150030

为明确大豆耐旱机制,以黑农44(耐旱型品种)和黑农65(敏感型品种)为试验材料,采用盆栽方式,于大豆初花期作胁迫处理,而后复水。研究不同土壤含水率及复水后抗坏血酸-谷胱甘肽(ASA-GSH)循环中抗氧化物质含量及抗氧化酶活性变化。结果表明... 详细信息

为明确大豆耐旱机制,以黑农44(耐旱型品种)和黑农65(敏感型品种)为试验材料,采用盆栽方式,于大豆初花期作胁迫处理,而后复水。研究不同土壤含水率及复水后抗坏血酸-谷胱甘肽(ASA-GSH)循环中抗氧化物质含量及抗氧化酶活性变化。结果表明,土壤含水率降低改变ASA-GSH循环非酶和酶成分,诱导丙二醛(MDA)积累。ASA-GSH循环中,干旱胁迫处理下还原型抗坏血酸(ASA)、还原型谷胱甘肽(GSH)含量先升后降,脱氢抗坏血酸(DHA)、氧化型谷胱甘肽(GSSG)含量呈升高趋势,ASA/DHA呈先升后降趋势,GSH/GSSG呈高-低-高-低变化趋势,复水后ASA、DHA、GSH含量升高,GSSG含量下降。抗坏血酸过氧化物酶(APX)、单脱氢抗坏血酸还原酶(MDHAR)、脱氢抗坏血酸还原酶(DHAR)、谷胱甘肽还原酶(GR)活性呈先升后降趋势,复水后APX、GR、MDHAR、DHAR活性升高。黑农44抗氧化物质含量和抗氧化酶活性高于黑农65,MDA含量低于黑农65。研究为大豆抗旱筛选及抗氧化机制探究提供理论基础。

关键词：大豆土壤含水率 ASA-GSH循环复水

干旱程度及时期对复水后大豆生长和代谢补偿效应的影响

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农业工程学报 2015年第11期31卷 150-156页

作者：王利彬祖伟董守坤刘丽君徐亚会李雪凝东北农业大学农学院哈尔滨150030

为探讨大豆干旱胁迫及复水生长补偿效应和代谢补偿效应机制，建立最佳节水灌溉模式，采用盆栽称质量控水法，研究了干旱胁迫及复水对大豆生长发育、渗透调节、内源激素调节和抗氧化系统的影响。结果表明：干旱胁迫抑制株高和叶面积增长... 详细信息

为探讨大豆干旱胁迫及复水生长补偿效应和代谢补偿效应机制，建立最佳节水灌溉模式，采用盆栽称质量控水法，研究了干旱胁迫及复水对大豆生长发育、渗透调节、内源激素调节和抗氧化系统的影响。结果表明：干旱胁迫抑制株高和叶面积增长，在苗期和开花期复水天数为0时降幅分别达12.50%～39.74%、21.71%～52.87%和16.93%～36.27%、27.99%～47.80%；导致脯氨酸含量、可溶性糖含量和丙二醛含量升高，在苗期和开花期复水后天数为0的增幅分别达46.97%～131.57%、99.56%～139.37%、6.64%～22.82%和31.68%～136.30%、32.63%～184.51%、15.82%～100.75%；超氧化物歧化酶活性升高，但重度胁迫10 d处理呈降低趋势；脱落酸含量升高，赤霉素和玉米素核苷含量降低，而吲哚乙酸含量苗期呈降低趋势，开花期呈升高趋势。复水后，株高和叶面积日增长量表现出短暂快速增长，产生生长超补偿效应；中度胁迫和胁迫5 d处理各代谢指标产生等量或近等量代谢补偿效应，而重度胁迫和胁迫10 d处理产生近等量或部分代谢补偿效应。因此，干旱胁迫后复水产生补偿效应是有条件的，重度胁迫尤其是长时间重度胁迫会降低补偿效应甚至产生伤害效应。同时苗期适度干旱处理可提高开花期再次受旱的适应能力和补偿效应。

关键词：干旱胁迫生长大豆复水补偿效应

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“农学概论”在非农学专业开设的改革与探索

高等农业教育 2022年第3期 95-100页

作者：杨亮贾琰董守坤东北农业大学农学院黑龙江哈尔滨150030 东北农业大学黑龙江哈尔滨150030

新农科背景下如何培养具有创新意识和创新能力的涉农人才,如何提升学生的科研素养,如何培养社会需要的应用型人才,如何为乡村振兴计划提供人才,已成为高等农业院校亟待思考和解决的问题。“农学概论”课程在农业院校的非农学专业开设,... 详细信息

新农科背景下如何培养具有创新意识和创新能力的涉农人才,如何提升学生的科研素养,如何培养社会需要的应用型人才,如何为乡村振兴计划提供人才,已成为高等农业院校亟待思考和解决的问题。“农学概论”课程在农业院校的非农学专业开设,旨在普及相关农业知识,并将所学专业与农业有效结合。对课程内容体系、教师团队、课程考核体系等方面进行了全面深入的改革与探索,通过改革提高了教师授课质量,补充拓展了相关教学内容,丰富了教学手段,提高了学生学习兴趣和热情,学生反馈教学效果显著提高。

关键词：农学概论非农专业教学内容教学方法