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Overexpression of the maize GRF10, an endogenous truncated growth-regulating factor protein, leads to reduction in leaf size and plant height

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Journal of Integrative Plant Biology 2014年第11期56卷 1053-1063页

作者： Lei Wu Dengfeng Zhang Ming Xue Jianjun Qian Yan He shoucai wang National Maize Improvement Center of China Key Laboratory of Maize Biology and Genetic Breeding of Ministry of AgricultureChina Agricultural University Institute of Crop Science The Chinese Academy of Agricultural Sciences

It has long been thought that growth‐regulating factors（GRFs） gene family members act as transcriptional activators to play important roles in multiple plant developmental processes. However, the recent characterization of Arabidopsis GRF7 showed that it functions as a transcriptional repressor of osmotic stress‐responsive genes. This highlights the complex and diverse mechanisms by which different GRF members use to take action. In this study, the maize（Zea mays L.） GRF10 was functionally characterized to improve this concept. The deduced ZmGRF10 protein retains the N‐terminal QLQ and WRC domains, the characteristic regions as protein‐interacting and DNA‐binding domains, respectively. However,it lacks nearly the entire C‐terminal domain, the regions executing transactivation activity. Consistently, ZmGRF10 protein maintains the ability to interact with GRF‐interacting factors（GIFs） proteins, but lacks transactivation *** of ZmGRF10 in maize led to a reduction in leaf size and plant height through decreasing cell proliferation,whereas the yield‐related traits were not affected. Transcriptome analysis revealed that multiple biological pathways were affected by ZmGRF10 overexpression, including a few transcriptional regulatory genes, which have been demonstrated to have important roles in controlling plant growth and development. We propose that ZmGRF10 aids in fine‐tuning the homeostasis of the GRF‐GIF complex in the regulation of cell proliferation.

关键词： GIF growth-regulating factor maize overexpression RNA-Seq transcriptional activator

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Construction of a genetic map and location of quantitative trait loci for dwarf trait in maize by RFLP markers

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Chinese Science Bulletin 2000年第3期45卷 247-250页

作者： CAO Yongguo wang Guoying wang shoucai WEI Yanling LU Jiang XIE Youju DAI Jingrui College of Plant Science and Technology Chinese Agricultural UniversityBeijing 100094China National Laboratories for Agrobiotechnology Chinese Agricultural UniverysityBeijing 100094China Florida A&M University TallahasseeFL 32307USA

Using F2 population derived from the cross of tall inbred 7922 by dwarf inbred 5003, an RFLP linkage map of maize has been constructed, on which 85 markers are distributed among 10 linkage groups and span maize genome about 1827.8 cM with an average distance (24.4 cM) between markers. 106 F2:3 lines of the population were grown in a 10×11 simple rectangular lattice design of one-raw plots with two replications and evaluated for plant height (PH). With interval mapping procedure, 5 QTLs controlling plant height have been identified and their genetic effects and gene action determined. 2 major OTLs with opposite effect have been discovered. One for increasing plant height is ph 1 which is located at chromosome 2 and accounts for 51.8% of the total phenotypic variation; the other for decreasing plant height is ph3 which is located at chromosome 5 and accounts for 38.6% of the total phenotypic variation. The chromosomal location of ph3 might be the same as or close to the position of bv1, a dwarf mutant

关键词： Zea mays L. RFLP genetic map dwarf trait major QTL.

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Fine Mapping of RppP25, a Southern Rust Resistance Gene in Maize

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Journal of Integrative Plant Biology 2013年第5期55卷 462-472页

作者： Panfeng Zhao Guobin Zhang Xiaojun Wu Na Li Dianyi Shi Dengfeng Zhang Chunfang Ji Mingliang Xu shoucai wang National Maize Improvement Center of China Key Laboratory of Biology and Genetic Improvement of Maize (Ministry of Agriculture) China Agricultural University National Key Facility for Crop Gene Resources and Genetic Improvement (NFCRI) Institute of Crop ScienceChinese Academy of Agricultural Sciences

Southern rust （Puccinia polysora Underw.） is a major disease that can cause severe yield losses in maize （Zea mays L.）. In our previous study, a major gene RppP25 that confers resistance to southern rust was identified in inbred line P25. Here, we report the fine mapping and candidate gene analysis of RppP25 from the near-isogenic line F939, which harbors RppP25 in the genetic background of the susceptible inbred line F349. The inheritance of resistance to southern rust was investigated in the BC1F1 and BC3F1 populations, which were derived from a cross between F939 and F349 （as the recurrent parent）. The 1：1 segregation ratio of resistance to susceptible plants in these two populations indicated that the resistance is controlled by a single dominant gene. Ten markers, including three simple sequence repeat （SSR） markers and seven insertion/deletion （InDel） markers, were developed in the RppP25 region. RppP25 was delimited to an interval between P091 and M271, with an estimated length of 40 kb based on the physical map of B73. In this region, a candidate gene was identified that was predicted to encode a putative nucleotide-binding site leucine-rich repeat （NBS-LRR） protein. Two co-segregated markers will aid in pyramiding diverse southern rust resistance alleles into elite materials, and thereby improve southern rust resistance worldwide.

关键词： Fine mapping maize Puccinia polysora resistance gene southern rust.

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新型无耳托板螺母拉铆过程数值仿真与试验验证

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中国机械工程 2023年第7期34卷 875-881页

作者：王守财孙昂刘如刚中国航空工业集团公司沈阳飞机设计研究所沈阳110035 西安智同航空科技有限公司西安710000

一种新型304不锈钢无耳托板螺母的安装效率较高且能减少疲劳源,在航空领域应用广泛。基于Johnson-Cook模型建立了有限元模型,对该螺母拉铆成形过程载荷变化趋势、塑性流动、夹紧力与干涉量、夹层状态对铆接质量的影响分别进行分析,并开... 详细信息

一种新型304不锈钢无耳托板螺母的安装效率较高且能减少疲劳源,在航空领域应用广泛。基于Johnson-Cook模型建立了有限元模型,对该螺母拉铆成形过程载荷变化趋势、塑性流动、夹紧力与干涉量、夹层状态对铆接质量的影响分别进行分析,并开展了螺母的铆接安装试验。结果表明:有限元仿真模型与试验载荷曲线符合性较好,最大载荷误差为2.4%;螺母在受压应力远高于材料屈服应力时才发生结构失稳,迅速形成褶皱,塑性流动主要沿径向发展;拉铆过程可以给夹层提供一定量的夹紧力与干涉量,夹紧力在完成铆接后达到最大值,且在一定范围内夹层的斜度不影响夹紧力的大小,铆接过程最大干涉量为4.6%左右。

关键词： 304不锈钢无耳托板螺母 Johnson-Cook模型拉铆

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金属蒙皮飞机冲击威胁研究

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振动与冲击 2018年第4期37卷 13-18页

作者：王守财关志东黎增山黄骁北京航空航天大学航空科学与工程学院北京100191

飞机服役中会遭受外来物冲击威胁,对机身蒙皮造成冲击损伤。分别对国内南北方两航空公司的飞机维修记录进行了调研,得到了金属蒙皮飞机飞行过程中的冲击损伤分布规律;基于ABAQUS软件建立有限元模型,采用不含损伤的Johnson-Cook本构模型... 详细信息

飞机服役中会遭受外来物冲击威胁,对机身蒙皮造成冲击损伤。分别对国内南北方两航空公司的飞机维修记录进行了调研,得到了金属蒙皮飞机飞行过程中的冲击损伤分布规律;基于ABAQUS软件建立有限元模型,采用不含损伤的Johnson-Cook本构模型,研究铝板的低速冲击响应特性,设计并实施铝板低速冲击试验,对模型可用性进行了验证;得到了凹坑深度逆向推导冲击能量的计算公式,将损伤凹坑深度数据转化为冲击能量,并估算在一定概率水平下,金属蒙皮飞机可能遇到的最大冲击能量。结果显示,冲击能量较小时,铝板凹坑深度与冲击能量近似为线性关系;在指定的概率水平下,机翼、机身、翼身整体的最大冲击能量分别为21.3 J,25.5 J,33.8 J。

关键词：冲击威胁损伤分布凹坑概率

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Cloning and characterization of a novel R1-MYB transcription factor in maize

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Progress in Natural Science:Materials International 2009年第9期19卷 1089-1096页

作者： Guanqing Jia, Bo Li, Dengfeng Zhang, Tifu Zhang, Zhiyong Li, Jingrui Dai, shoucai wang* National Maize Improvement Center of China, Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry, Department of Plant Genetics & Breeding, China Agricultural University, Beijing 100193, China National Maize Improvement Center of China Key Laboratory of Crop Genomics and Genetic Improvement of Agriculture Ministry Department of Plant Genetics & Breeding China Agricultural University Beijing 100193 China

R1-MYB transcription regulatory factors play vital roles in transcriptional modification during higher plant metabolism and development. In the present study, an R1-MYB gene was isolated from the maize (Zea mays L.) inbred line C8605-2 based on expressed sequence tags (ESTs), which expressed differentially between the hybrid C8605-2 × W1445 and its two parents in an oligonucleotide microarray. The full-length cDNA, designated as ZmMYBE1 (GeneBank Accession No. FJ024049), consists of 1992 nucleotides and contains a 1296-bp open reading frame. One conserved MYB domain was detected near the N-terminus of the deduced amino acid sequence, where an acidic Ser/Thr-rich area was also observed in the downstream region. The sequence of ZmMYBE1, mapped to chromosome 5 (bin 5.03), was revealed belonging to a multi-gene family in the maize genome. Subcellular localization analysis determined that ZmMYBE1 was located in the nucleus. Expression analysis showed that ZmMYBE1 transcripts accumulated in various tissues examined, with high expression levels in the immature ear and low levels in the tassel, root, and stem. The expression analysis for ZmMYBE1 in immature ears showed the highest expression level at the earliest vegetative developmental stage of the plant. Expression of ZmMYBE1 higher than that of the parents was observed in the hybrid C8605-2 × W1445. And also the expression level similar to the lowest of the parents in the hybrid C8605-2 × W245 was observed, revealing different expression patterns for the same gene in various hybrids combined from diversified inbred lines. Transgenic Arabidopsis overexpressing ZmMYBE1 implies that ZmMYBE1 has an important role in developmental regulation in maize.

关键词： MYB transcription factor Differential expression Maize

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公平科学公正推进玉米产业发展

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中国种业 2007年第7期 21-23页

作者：季广德王守才孙世贤内蒙古自治区种子管理站呼和浩特010010 中国农业大学国家玉米改良中心全国农业技术推广服务中心

根据《中华人民共和国种子法》（以下简称《种子法》）和农业部《主要农作物品种审定办法》规定，2002年第一届国家农作物品种审定委员会成立。在国家农作物品种审定委员会办公室、农业部种植业管理司和全国农业技术推广服务中心的正确... 详细信息

根据《中华人民共和国种子法》（以下简称《种子法》）和农业部《主要农作物品种审定办法》规定，2002年第一届国家农作物品种审定委员会成立。在国家农作物品种审定委员会办公室、农业部种植业管理司和全国农业技术推广服务中心的正确领导下，玉米专业委员会全体委员依法开展了品种审定工作，圆满完成了工作任务。

关键词：农作物品种审定委员会产业发展《中华人民共和国种子法》玉米农业技术推广服务中心公正科学品种审定工作

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镇江内江湿地植物群落演替过程中土壤养分动态研究

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生态环境 2007年第5期16卷 1475-1480页

作者：陈海霞付为国王守才卞新民南京农业大学区域农业研究所江苏南京210095 江苏大学农业工程研究院江苏镇江212013 南京市六合区竹镇镇农业服务中心江苏南京211501

镇江内江湿地是由长江携带的泥沙在镇江内江特有的水文条件下淤积而成,形成于江滩裸地上的植物群落开始了群落的原生演替过程。在此过程中,湿地的土壤养分状况随着植被的演替更迭,发生着协同演化的过程。为了进一步丰富群落演替理论,通... 详细信息

镇江内江湿地是由长江携带的泥沙在镇江内江特有的水文条件下淤积而成,形成于江滩裸地上的植物群落开始了群落的原生演替过程。在此过程中,湿地的土壤养分状况随着植被的演替更迭,发生着协同演化的过程。为了进一步丰富群落演替理论,通过研究群落演替过程中不同阶段以及同一演替阶段不同层次土壤养分状况的时空变化,探索演替过程中的不同植被类型和土壤养分变化的内在联系及其互作效应。结果表明,随着演替进行,土壤的养分状况趋于改善,土壤有机质、全氮和速效磷含量呈上升趋势,只有速效钾的含量在后期芦苇阶段明显降低,这主要是由于收割芦苇将富含钾素的芦苇植株带出湿地系统所致。由于植物根系分布及枯落物多少的差异,使得各营养成分的垂直分布各异。

关键词：群落演替有机质全氮速效磷速效钾

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