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Overexpression of a Potato Sucrose Synthase Gene in Cotton Accelerates Leaf Expansion, Reduces Seed Abortion, and Enhances Fiber Production

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Molecular Plant 2012年第2期5卷 430-441页

作者： shou-min xu Elizabeth Brill Danny J. Llewellyn Robert T. Furbank Yong-Ling Ruan CSIRO Plant Industry GPO Box 1600 Canberra ACT 2601 Australia School of Environmental and Life Sciences University of Newcastle Newcastle NSW 2308 Australia

Sucrose synthase （Sus） is a key enzyme in the breakdown of sucrose and is considered a biochemical marker for sink strength, especially in crop species, based on mutational and gene suppression studies. It remains elusive, however, whether, or to what extent, increase in Sus activity may enhance sink development. We aimed to address this question by expressing a potato Sus gene in cotton where Sus expression has been previously shown to be critical for normal seed and fiber development. Segregation analyses at T1 generation followed by studies in homozygous progeny lines revealed that increased Sus activity in cotton （1） enhanced leaf expansion with the effect evident from young leaves emerging from shoot apex; （2） improved early seed development, which reduced seed abortion, hence enhanced seed set, and （3） promoted fiber elongation. In young leaves of Sus overexpressing lines, fructose concentrations were significantly increased whereas, in elongating fibers, both fructose and glucose levels were increased. Since hexoses contribute little to osmolality in leaves, in contrast to developing fibers, it is concluded that high Sus activity promotes leaf development independently of osmotic regulation, probably through sugar signaling. The analyses also showed that doubling the Sus activity in 0-d cotton seeds increased their fresh weight by about 30%. However, further increase in Sus activity did not lead to any further increase in seed weight, indicating an upper limit for the Sus overexpression effect. Finally, based on the observed additive effect on fiber yield from increased fiber length and seed number, a new strategy is proposed to increase cotton fiber yield by improving seed development as a whole, rather than solely focusing on manipulating fiber growth.

关键词： cotton fiber invertase leaf expansion seed abortion seed development sucrose synthase sugar signaling.

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A Vector Construction for High Oleic and Selectable Marker Free Sunflower (Helianthus annuus L)

A Vector Construction for High Oleic and Selectable Marker F...

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中国遗传学会植物遗传与基因组学专业委员会2005年学术研讨会

作者： Tai-Cheng Jin, Wei Chen, shou-min xu and Li-Xia Liu School of Life Sciences, Northeast Normal University Changchun 130024 China

Sunflower seed oil is the forth-largest source of plant oil in the world. It is typically composed of about 23.8% oleic acid (C18: 1) and 65% linoleic acid (C18:0). Compared to linoleic acid, oleic acid is more stable and healthful, which could reduce the harmful LDL content in the bloodstream and the risk of suffering cardiovascular disease. So increasing the relative proportions of oleic acid in sunflower seed oil is meaningful. Many studies demonstrate that the oledyl-phosphatidylcholine ω-6 desaturase (FAD2) play a role of converting oleic acid to linoleic acid, and the depression of its activity will lead to a large amount of oleic acid accumulated. Here, we employed intron-spliced hairpin (iHP) RNA-mediated gene silencing technique to down-regulate the expression of haFAD2-1, a key gene for oleic acid metabolism in sunflower seeds, hoping to increase the oleic oil content correspondingly.

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Established And Optimized A High Efficiency Embryonic Tip Regeneration System In Soybean

Established And Optimized A High Efficiency Embryonic Tip Re...

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中国遗传学会植物遗传与基因组学专业委员会2005年学术研讨会

作者： Wang-Feng Li, Yi-Rong Cai, Kai Song, shou-min xu and Li-Xia Liu School of Life Sciences, Northeast Normal University Changchun 130024 China

Soybean (Glycine Max) is a very important source of vegetable oil and protein for animal and human foods. Recently, transgenic soybean cultivars have grown in a large scale around world. However genetic transformation of soybean is still difficult because of low regeneration and transformation efficiency. Establishment of a high efficient regeneration system in soybean is very important. In this study, we have established and optimized a higher frequency embryonic tip regeneration system and obtained more than 50 regeneration soybean lines successfully using this embryonic tip regeneration system.

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Small extracellular vesicles derived from human induced pluripotent stem cell-differentiated neural progenitor cells mitigate retinal ganglion cell degeneration in a mouse model of optic nerve injury

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Neural Regeneration Research 2025年第2期20卷 587-597页

作者： Tong Li Hui-min Xing Hai-Dong Qian Qiao Gao Sheng-Lan xu Hua Ma Zai-Long Chi State Key Laboratory of Ophthalmology Optometry and Visual ScienceEye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina National Clinical Research Center for Ocular Diseases Eye HospitalWenzhou Medical UniversityWenzhouZhejiang ProvinceChina

Several studies have found that transplantation of neural progenitor cells(NPCs)promotes the survival of injured ***,a poor integration rate and high risk of tumorigenicity after cell transplantation limits their clinical *** extracellular vesicles(sEVs)contain bioactive molecules for neuronal protection and *** studies have shown that stem/progenitor cell-derived sEVs can promote neuronal survival and recovery of neurological function in neurodegenerative eye diseases and other eye *** this study,we intravitreally transplanted sEVs derived from human induced pluripotent stem cells(hiPSCs)and hiPSCs-differentiated NPCs(hiPSC-NPC)in a mouse model of optic nerve *** results show that these intravitreally injected sEVs were ingested by retinal cells,especially those localized in the ganglion cell *** with hiPSC-NPC-derived sEVs mitigated optic nerve crush-induced retinal ganglion cell degeneration,and regulated the retinal microenvironment by inhibiting excessive activation of *** analysis further revealed that hiPSC-NPC derived sEVs transported neuroprotective and anti-inflammatory miRNA cargos to target cells,which had protective effects on RGCs after optic nerve *** findings suggest that sEVs derived from hiPSC-NPC are a promising cell-free therapeutic strategy for optic neuropathy.

关键词： exosome miRNA neural progenitor cell neurodegeneration neuroinflammation neuroprotection optic nerve crush optic neuropathy retinal ganglion cell small extracellular vesicles

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Modular assembly of complex C-alkyl glycoside enabled via cooperative NHC and photoredox catalysis

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Science China Chemistry 2025年

作者： Rui-Qiang Jiao ming Li Xi Chen Mei-Ze xu Tian Ding Xiao-Ping Gong Zhuo-Mei Li xue-Yuan Liu Yong-min Liang State Key Laboratory of Applied Organic Chemistry Lanzhou University College of Chemistry and Chemical Engineering Northwest Normal University

C-Alkyl glycosides are found in natural products and are indispensable in drug development programs,owing to their diverse range of medicinal ***-component reactions involving glycosyl radical addition are well documented and proved efficient for the C-alkyl glycosides ***,multicomponent reactions for the rapid and controllable construction of structurally diversified C-alkyl glycosides in a single step remains a significant challenge due to the issues associated with balancing reactivity,compatibility,and ***,we report an NHC and photoredox co-catalyzed three-component method to access complex and valuable C-alkyl *** protocol exhibits broad substrate scope and excellent chemoand ***,nine types of sugars are compatible with this *** addition,the generality of this catalytic strategy was further highlighted by the potential for scalable production,the feasibility of further modifications of the products and its successful application in the late-stage functionalization of pharmaceutical skeletons.

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传粉昆虫的研究现状及存在的问题

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西南农业学报 2001年第4期14卷 102-108页

作者：郭柏寿杨继民许育彬西北农林科技大学陕西杨陵712100

传粉昆虫种类繁多 ,其传粉特性和效果各不相同。目前 ,国外关于蜜蜂、熊蜂、壁蜂对农作物授粉的研究较为系统 ,措施得当 ,且已建立了工厂化周年繁育和出售的专业公司。国内对传粉昆虫的研究较为滞后 ,虽然在野生种类的驯养、人工繁殖方... 详细信息

传粉昆虫种类繁多 ,其传粉特性和效果各不相同。目前 ,国外关于蜜蜂、熊蜂、壁蜂对农作物授粉的研究较为系统 ,措施得当 ,且已建立了工厂化周年繁育和出售的专业公司。国内对传粉昆虫的研究较为滞后 ,虽然在野生种类的驯养、人工繁殖方面取得突破 ,但要实现规模化饲养还存在不少问题。尤其要在传粉昆虫资源调查、生物学和生态学研究、人工繁殖、定向育种、病虫害防治、野生资源驯化和管理、授粉技术的改进等方面的研究上投入更大的力量 ,尽快缩短与发达国家的距离 ,让传粉昆虫更好地服务于农业生产。

关键词：传粉昆虫种类壁蜂经济效益人工驯养

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Primary parenchymal squamous cell carcinoma of the kidney:A case report

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World Journal of Clinical Cases 2025年第4期13卷 34-40页

作者： Zhi-Hui Zheng Bo Shao Chao-min xu Ke Wang Jia-Zhu Wen Li-Kang Luo Jia-Cheng Guan Department of Ultrasound The Second People’s Hospital of QuzhouQuzhou 324000Zhejiang ProvinceChina Department of Radiology Shulan(Quzhou)HospitalQuzhou 324000Zhejiang ProvinceChina Department of Radiology The Second People’s Hospital of QuzhouQuzhou 324000Zhejiang ProvinceChina Department of Pathology The Second people’s Hospital of QuzhouQuzhou 324000Zhejiang ProvinceChina

BACKGROUND Primary squamous cell carcinoma(SCC)of the renal parenchyma is extremely rare,with only nine cases *** SUMMARY This study reports a 51-year-old man with primary SCC of the renal *** patient was admitted with recurrent dull pain and discomfort in the right lumbar region,which had worsened over 2 weeks,accompanied by painful gross *** antigen(SCCA)levels were elevated,and imaging revealed a renal mass with associated *** patient underwent laparoscopic unilateral nephrectomy and lymph node *** pathology confirmed highly differentiated SCC with necrosis in the right renal parenchyma,with negative renal pelvis and *** pathological stage was *** months after surgery,the tumor recurred with involvement of the liver,right psoas major muscle,and inferior vena *** patient refused chemotherapy and succumbed to the disease 6 months postoperatively due to disease *** We report a case of primary SCC of the renal parenchyma,a rare renal *** clinical symptoms,laboratory tests,and imaging findings are nonspecific,making accurate and timely diagnosis *** to the literature,for patients with renal calculi accompanied by a renal mass,elevated serum SCCA levels,and magnetic resonance imaging showing cystic or cystic-solid masses within the kidney with pseudocapsules and heterogeneous mild enhancement,the possibility of this disease should be considered.

关键词： Renal tumor Renal parenchyma Squamous cell carcinoma Renal calculi Computed tomography Case report

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Effects of nature organic matters and hydrated metal oxides on the anaerobic degradation of lindane,p,p'-DDT and HCB in sediments

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Journal of Environmental Sciences 2003年第5期15卷 618-621页

作者： ZHAO xu QUAN Xie ZHAO Hui min CHEN Jing wen CHEN shou ZHAO Ya zhi School of Environmental Science and Technology Dalian University of TechnologyDalian 116012China.

Effects of natural organic matters(NOM) and hydrated metal oxides(HMO) in sediments on the anaerobic degradation of γ 666, p,p' DDT and HCB were investigated by means of removing NOM and HMO in Liaohe River sediments sequentially. The results showed that the anaerobic degradation of γ 666, p,p' DDT and HCB followed pseudo first order kinetics in different sediments. But, the extents and rates of degradation were different, even the other conditions remained the same. Anaerobic degradation rates of γ 666, p,p' DDT and HCB were 0 020 d -1 , 0 009 d -1 and 0 035 month -1 respectively for the sediments without additional carbon resources. However, with addition of carbon resources, the anaerobic degradation rates of γ 666, p, p ' DDT and HCB were 0 071 d -1 , 0 054 d -1 and 0 088 month -1 in the original sediments respectively. After removing NOM, the rates were decreased to 0 047 d -1 , 0 037 d -1 and 0 066 month -1 ; in the sediments removed NOM and HMO, the rates were increased to 0 067 d -1 , 0 059 d -1 and 0 086 month -1 . These results indicated that NOM in the sediments accelerated the anaerobic degradation of γ 666, p,p' DDT and HCB; the HMO inhibited the anaerobic degradation of γ 666, p,p' DDT and HCB.

关键词： lindane p,p' DDT HCB anaerobic degradation sediments natural organic matters

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Experimental study of the separated joint of an underground pipeline rehabilitated by cured-in-place pipe

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Underground Space 2022年第4期7卷 543-563页

作者： Jui-min Hsu Keh-Jian shou Department of Civil Engineering Chung-Hsing UniversityTaiwanChina

Reinforced concrete pipes(RCPs)were commonly used in older underground pipelines in Taiwan,***,their joints are prone to damage,including fracture and separation,from problems such as overloading,poor backfilling,and *** the rehabilitation of such aged pipelines,trenchless methods are preferred to minimize the impact on *** cured-in-place-pipe(CIPP)rehabilitation could be one of the most popular trenchless methods suitable for the cases with angular alignment and changing ***,according to the existing standards,no details are available on the performance of the rehabilitated parts of the damaged pipeline,including the effect of separated *** study designed and conducted laboratory testing on the composite pipe joint(the CIPP rehabilitated RCPs with a separated joint)with different scenarios,including radial and longitudinal loading *** radial test results indicated that,for a small separation distance,CIPP rehabilitation can restore the original function of a pipeline and improve its hoop *** longitudinal test revealed that the damage due to bending was controlled by the tensile strength of the liner *** ultimate flexural strength decreased as the separation distance ***,pipelines may be subjected to additional loads due to flotation or subsidence,for which the longitudinal loading capacity is often the limiting *** such conditions,it is necessary to confirm,by more research,whether the wall thickness is sufficient.

关键词： Reinforced concrete pipes Pipe joint Rehabilitation Cured-in-place pipe Trenchless technology

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Effects of Root Pruning on Non-Hydraulic Root-Sourced Signal, Drought Tolerance and Water Use Efficiency of Winter Wheat

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Journal of Integrative Agriculture 2013年第6期12卷 989-998页

作者： MA shou-chen LI Feng-min YANG Shen-jiao LI Chun-xi xu Bing-cheng ZHANG xu-cheng State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau Ministry of Water Resources/Institute of Soil and Water Conservation Chinese Academy of Sciences School of Surveying and Land Information Engineering Henan Polytechnic University Key Laboratory of Efficient Utilization of Water in Dry Farming Gansu Province College of Life Science Henan Normal University

Two pot experiments were conducted to study the effects of root pruning at the stem elongation stage on non-hydraulic root-sourced signals （nHRS）, drought tolerance and water use efficiency of winter wheat （Triticum aestivum）. The root pruning significantly reduced the root weight of wheat, but had no effect on root/shoot ratio at the two tested stages. At booting stage, specific root respiration of root pruned plants was significantly higher than those with intact roots （1.06 and 0.94 mmol g-1 s-1, respectively）. The soil water content （SWC） at which nHRS for root pruned plants appeared was higher and terminated lower than for intact root plants, the threshold range of nHRS was markedly greater for root pruned plants （61.1-44.6% field water capacity） than for intact root plants （57.9-46.1% field water capacity）. At flowering stage, while there was no significant difference in specific root respiration. The SWCs at which nHRS appeared and terminated were both higher for root pruned plants than for intact root plants. The values of chlorophyll fluorescence parameters, i.e., the effective photosystem II quantum yield （F PS II ）, the maximum photochemical efficiency of PS II （F v /F m ）, coefficient of photochemical quenching （qP）, and coefficient of non-photochemical quenching （NPQ）, in root pruned plants were significantly higher than in intact root plants, 7 d after withholding of water. Root pruned plants had significantly higher water use efficiency （WUE） than intact root plants in well-watered and medium drought soil, but not in severe drought condition. In addition, root pruning had no significant effect on grain yield in well-watered and medium drought soil, but significantly decreased grain yield in severe drought condition. In conclusion, the current study showed that root pruning significantly altered nHRS sensitivity and improved WUE of winter wheat in well-watered and medium drought soil, but lowered drought tolerance of winter wheat in severe drought soil

关键词： winter wheat root pruning non-hydraulic root-sourced signal (nHRS) root activity drought tolerance

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