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Ultrasound guided testicular fine needle aspiration in buck (Capra hircus)-An animal model

Asian pacific Journal of Reproduction 2017年第1期6卷 26-31页

作者： Sattwikesh Paul Nasrin Sultana Juyena shonkor kumar das Raihana Nasrin Ferdousy Rahul Deb Sarker Soheli Jahan Mou Department of Surgery and Radiology Faculty of Veterinary Medicine and Animal ScienceBangabandhu Sheikh Mujibur Rahman AgriculturalUniversityGazipurBangladesh Department of Surgery and Obstetrics Faculty of Veterinary ScienceBangladesh Agricultural UniversityMymensingh Department of Anatomy and Histology Faculty of Veterinary ScienceBangladesh Agricultural UniversityMymensingh Department of Pathology Faculty of Veterinary ScienceBangladesh Agricultural UniversityMymensingh.

Objective:To establish ultrasound guided testicular fine needle aspiration (TFNA) as well as to assess the effectiveness of uni-directional (UD) and multi-directional (MD) TFNA in buck according to testicular cells, echotexture and gross changes of testicle, age of buck was ***:A total of 120 samples were collected with both directions (UD, n=60) and (MD,n=60) suction from testis of 10 apparently healthy bucks. All slides were stained with May-Grünwald-Giemsa and examined under light microscope with 1000× magnifications to count spermatogenic cells, spermatozoa and sertoli cells. The percentage of spermatozoa and sertoli cells were expressed as spermatic index and sertoli cell ***:Results revealed no difference in the presence of various spermatogenic and sertoli cells in cell cluster of slides made either unidirectional TFNA or multidirectional TFNA. Early spermatids were the most numerous, followed by late spermatids, primary spermatocytes, spermatogonia. Sertoli cell index was higher in TFNA smears of young bucks prepared from (7-13 mo) of age and spermatic index was higher in adult bucks (14-24 mo) of age. No echogenic change was observed in the echotexture of testisafter ***: It seems that TFNA has no serious ill effect on the buck testis when uni-direction aspiration is performed. Moreover, the possibility to standardize this method might provide a greater impulse to the clinical diagnostics of male animal infertility.

关键词： Testicular fine needle aspiration Spermatic index Sertoli cell index

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