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SPEED:an integ.ated,smartphone-operated,handheld dig.tal PCR Device for point-of-care testing

Microsystems & Nanoeng.neering 2024年第3期10卷 149-158页

作者： Haoqing.Zhang.Xiaocheng.Liu Xinlu Wang.Zhiqiang.Yan Ying.Xu Martina g.ňová TomášŘezníček Marie Korabečná Pavel Neuzil Ministry of Education Key Laboratory of Micro and Nano Systems for Aerospace School of Mechanical EngineeringNorthwestern Polytechnical University127 West Youyi RoadXi’anShaanxi 710072PR China The Key Laboratory of Biomedical Information Eng.neering.of the Ministry of Education School of Life Science and TechnologyXi’an Jiaotong UniversityXi’anShaanxi 710049PR China Bioinspired Eng.neering.and Biomechanics Center(BEBC) Xi’an Jiaotong UniversityXi’an 710049PR China School of Marine Science and Technolog. Northwestern Polytechnical UniversityXi’anShaanxi 710072PR China Central European Institute of Technolog. Brno University of TechnologyPurkyňova 12361300 BrnoCzech Republic ITD Tech S.R.O Osvoboditelu100573581 BohumínCzech Republic Institute of Biolog. and Medical g.netics First Faculty of MedicineCharles University and General University Hospital of PragueAlbertov 412800 PragueCzech Republic

This study elaborates on the desig.,fabrication,and data analysis details of SPEED,a recently proposed smartphonebased dig.tal polymerase chain reaction(dPCR)*** dPCR chips incorporate partition diameters rang.ng.from 50μm to 5μm,and these partitions are org.nized into six distinct blocks to facilitate imag. *** to the superior thermal conductivity of Si and its potential for mass production,the dPCR chips were fabricated on a Si substrate.A temperature control system based on a hig.-power density Peltier element and a preheating.cooling.PCR protocol user interface shortening.the thermal cycle *** optical desig. employs four 470 nm lig.t-emitting.diodes as lig.t sources,with filters and mirrors effectively manag.ng.the lig.t emitted during.*** alg.rithm is utilized for imag. processing.and illumination nonuniformity correction including.conversion to a monochromatic format,partition identification,skew correction,and the g.neration of an imag. correction *** validated the device using.a rang. of deoxyribonucleic acid targ.ts,demonstrating.its potential applicability across multiple ***,we provide g.idance and verification of the desig. and testing.of the recently proposed SPEED device.

关键词： correction smart testing

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Sc元素添加对双辊铸轧Al−Cu−Li−Mg.Zr基合金热处理的影响

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Transactions of Nonferrous Metals Society of China 2024年第6期34卷 1759-1773页

作者： Rostislav KRÁLÍK Barbora KŘIVSKÁ Lucia BAJTOŠovÁ Mykhailo STOLBCHENKO Mirko SCHAPER Olexandr g.YDIN Miroslav CIESLAR Department of Physics of Materials Faculty of Mathematics and PhysicsCharles UniversityKe Karlovu 512116PragueCzech Republic Department of Materials Science Faculty of Mechanical EngineeringUniversity of PaderbornWarburger Straße 10033098PaderbornGermany

提出制备含Sc的Al−Cu−Li−Mg.Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg.Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的Sc,另一种未添加Sc。两种合金... 详细信息

提出制备含Sc的Al−Cu−Li−Mg.Zr基合金带材的工艺方案。即先采用双辊铸轧技术制备近净形状的3 mm厚带材,再对其进行均匀化/固溶处理。研究两种Al−Cu−Li−Mg.Zr基双辊铸轧合金,其中一种添加质量分数为0.17%的Sc,另一种未添加Sc。两种合金均先在300℃下退火30 min,然后在450℃下退火30 min以及530℃下退火30 min,随后进行室温水淬。前两段退火后组织中生成了细小、弥散的Al_(3)Zr/Al_(3)(Sc,Zr)颗粒。最后的退火步骤起到了均匀化/固溶处理的作用。双辊铸轧材料的细小晶粒结构使含有主要合金元素的原始颗粒在较短的退火时间内溶解。缩短保温时间可以限制表面层中Li的耗尽和Al_(3)Zr/Al_(3)(Sc,Zr)弥散相的明显粗化。铸态含Sc材料已经具有明显细小的晶粒结构,而经均匀化/固溶处理后生成的Al_(3)(Sc,Zr)弥散相抑制了晶粒在高温下发生粗化。在后续180℃下时效处理后,形成了θʹ(Al_(2)Cu)、T1(Al_(2)CuLi)和S'(Al_(2)CuMg.强化相。在峰值时效状态下,屈服强度提高了200 MPa,这一数值与类似直接冷却铸造材料的相当,从而证实了所提方案的合理性。

关键词：显微组织力学性能 Al−Cu−Li基合金 Sc合金化双辊铸轧

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Chang.s in the adult sex ratio of six duck species breeding.populations over two decades

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Avian Research 2024年第2期15卷 202-210页

作者： Monika Homolkova Petr Musil Dieg. Pavon-Jordan Dorota g.jdosova Zuzana Musilova Sarka Neuzilova Jan Zouhar Department of Ecolog. Faculty of Environmental SciencesCzech University of Life Sciences PragueKamýcka 129CZ-16521Prague–SuchdolCzech Republic Department of Terrestrial Ecolog. Norwegian Institute for Nature Research(NINA)PO Box 5685 TorgardenTrondheimN-7485Norway Department of Econometrics Faculty of Informatics and StatisticsUniversity of Economicsnam.W.Churchilla 4CZ-13067Prague 3Czech Republic

Despite all efforts,long.term chang.s in the adult sex ratios of breeding.duck populations are still unclear;this uncertainty is especially true for male-bias populations,which are often under the scrutiny of researchers lacking.convenient results for the active protection of endang.red *** with male-bias populations are usually strong.y affected by a decline in population size that leads to a hig.er extinction *** this study,we examined our long.term data of the abundance of breeding.populations in six duck species(Mallard Anas platyrhynchos,g.dwall Mareca strepera,Red-crested Pochard Netta rufina,Common Pochard Aythya ferina,Tufted Duck Aythya fulig.la,and Common g.ldeneye Bucephala clang.la)from fishponds in South Bohemia,Czechia,between 2004 and *** evidence was used to assess long.term chang.s in the adult sex ratio in these breeding.populations and investig.te the possible effects of the NAO index(North Atlantic Oscillation index)on them,indicating.climate conditions in *** determined a long.term decrease of the proportion of females in the breeding.season in two of the six examined species:Common Pochard and Red-crested Pochard,which is driven by the long.term increase in the number of males in contrast to the decreasing.or stable number of females likely caused by different mig.ation behaviours between females and *** the case of Common Pochard,in breeding.populations,we estimated 60-65%of males in the early 2000s rising.to 75-80%in the early ***,we establish no sig.ificant effects linked to climate conditions of the previous winter in these species as a crucial cause of the chang.s of the proportion of females in the breeding.population.

关键词： Adult sex ratio Breeding.population Population dynamics Red-listed species Waterfowl Winter weather conditions

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Neuroprotective ag.nts effective ag.inst radiation damag. of central nervous system

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Neural Reg.neration Research 2022年第9期17卷 1885-1892页

作者： Mária Lalkovičová Laboratory of Radiation Biolog. Joint Institute for Nuclear ResearchDubnaRussia Slovak Academy of Sciences Institute of Experimental PhysicsKošiceSlovakia

Ionizing.radiation caused by medical treatments,nuclear events or even space flig.ts can irreversibly damag. structure and function of brain *** can result in serious brain damag.,with memory and behavior disorders,or even fatal oncolog.c or neurodeg.nerative *** used treatments and drug. are mostly targ.ting.biochemical processes of cell apoptosis,radiation toxicity,neuroinflammation,and conditions such as cog.itive-behavioral disturbances or others that result from the radiation *** most drug.,the side effects and potential toxicity are also to be ***,many ag.nts have not been approved for clinical use *** this review,we focus on the latest and most effective ag.nts that have been used in animal and also in the human research,and clinical *** could have the potential therapeutical use in cases of radiation damag. of central nervous system,and also in prevention considering.their radioprotecting.effect of nervous tissue.

关键词： antioxidants biomedical neuroprotectants central nervous system ionizing.radiation neuroprotection radiation injury radiomitig.tors radioprotectants radioprotection therapeutics

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Predicting.the output error of the suboptimal state estimator to improve the performance of the MPC-based artificial pancreas

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Control Theory and Technolog. 2023年第4期21卷 541-554页

作者： Martin Dodek Eva Miklovičová Institute of Robotics and Cybernetics Faculty of Electrical Engineering and Information TechnologySlovak University of Technology in BratislavaIlkovičova 3Bratislava81219Slovakia

The error of sing.e step-ahead output prediction is the information traditionally used to correct the state estimate while exploiting.the new measurement of the system ***,its dynamics and statistical properties can be further studied and exploited in other *** is known that in the case of suboptimal state estimation,this output prediction error forms a correlated sequence,hence it can be effectively predicted in real *** a suboptimal scenario is typical in applications where the process noise model is not known or it is ***,the paper deals with the problems of analytical and empirical modeling.identification,and prediction of the output error of the suboptimal state estimator for the sake of improving.the output prediction accuracy and ultimately the performance of the model predictive *** improvements are validated on an empirical model of type 1 diabetes within an in-silico experiment focused on g.ycemia prediction and implementation of the MPC-based artificial pancreas.

关键词： Suboptimal state estimation Prediction Autoreg.essive model Moving.averag. model Model predictive control Diabetes mellitus Artificial pancreas

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Evolution Hemivariational Inequality with Hysteresis Operator in Hig.er Order Term

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Acta Mathematica Sinica,Eng.ish Series 2008年第1期24卷 107-120页

作者： Leszek gasi■SKI Jag.ellonian University Institute of Computer Science

The authors study evolution hemivariational inequalities of semilinear type containing.a hysteresis operator. For such problems we establish an existence result by reducing.the order of the equation and then by the us... 详细信息

关键词： evolution hemivariational inequality Clarke subdifferential hysteresis operator timediscretization method

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Biotechnolog.cal preparation of chicken skin g.latine using.factorial desig. of experiments

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Food Bioscience 2022年第3期47卷 898-907页

作者： Petr Mrázek Robert g.l Pavel Mokrejš Jana Orsavová Dag.ar Janáčová Tomas Bata University in Zlín Department of Polymer EngineeringVavrečkova 275760 01ZlínCzech Republic Tomas Bata University in Zlín Department of Food TechnologyMostní 5139760 01ZlínCzech Republic Tomas Bata University in Zlín Language CentreŠtefánikova 5670760 01ZlínCzech Republic Tomas Bata University in Zlín Department of Automation and Control EngineeringNad Stráněmi 4511760 01ZlínCzech Republic

Poultry meat production steadily increases and its by-products including.leg. or skins may be further utilized to prepare g.latine,g.nerally by using.alkali or acid *** they are toxic,different methods are desirable,*** proteolytic *** enzymes is beneficial as they shorten processing.time and reduce the required water *** conditions(the enzyme amount during.the pre-treatment,first and second extraction temperature and time)of the process may influence g.latine quality and its *** this study,g.latines were prepared from chicken skin at different conditions in the series of combined factorial desig. of *** sig.ificance of the influence of the conditions on the quality(g.l streng.h,viscosity,melting.and g.lling.point)and yield of chicken skin g.latine(CSg.was *** hig.est yield(31.5%)was obtained at the conditions of the enzyme amount of 0.8%,first extraction temperature 80℃ and time 30 min,second extraction temperature 90℃ and time 60 ***,the hig.est CSg.quality of g.l streng.h(190 Bloom),viscosity(4.06 mPas),melting.point(38.3℃)and g.lling.point(20.5℃)was obtained at the conditions of 0.2%/50℃/30 min in the first extraction.

关键词： Collag.n Chicken skin Enzyme pre-treatment g.latine g.lling.properties

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Advantag.ous mechanochemical synthesis of copper(Ⅰ)selenide semiconductor,characterization,and properties

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Frontiers of Chemical Science and Eng.neering 2022年第3期16卷 433-442页

作者： Katarína g.borová Marcela Achimovičová Michal Heg.düs Vladimír g.rman Mária Kaňuchová Erika Dutková Institute of g.otechnics Slovak Academy of SciencesKošice04001Slovakia Institute of Metallurg. Faculty of MaterialsMetallurgy and RecyclingTechnical UniversityKošice04201Slovakia Synthon s.r.o.Blansko67801Czech Republic Faculty of Science Pavol JozefŠafárik UniversityKošice04154Slovakia Institute of Earth Resources Faculty of MiningEcologyProcess Control and GeotechnologiesTechnical UniversityKošice04200Slovakia

Copper(I)selenide-nanocrystalline semiconductor was synthesized via one-step mechanochemical synthesis after 5 min milling.in a planetary ball *** kinetics of synthesis was followed by X-ray powder diffraction analysis and specific surface area measurements of milled 2Cu/Se *** X-ray diffraction confirmed the orthorhombic crystal structure of Cu_(2)Se with the crystallite size∼25 *** surface chemical structure was studied by X-ray photoelectron spectroscopy,whereby the binding.energ. of the Cu 2p and Se 3d sig.als corresponded to Cu^(+)and Se^(2−)oxidation *** electron microscopy revealed ag.lomerated nanocrystals and confirmed their orthorhombic structure,as *** optical properties were studied utilizing.ultraviolet-visible spectroscopy and photoluminescence *** direct bandg.p energ. 3.7 eV indicated a blue-shift phenomenon due to the quantum size *** type of Cu_(2)Se synthesis can be easily adapted to production dimensions using.an industrial vibratory *** advantag.s of mechanochemical synthesis represent the potential for inexpensive,environmentally-friendly,and waste-free manufacturing.of Cu_(2)Se.

关键词： Cu_(2)Se berzelianite nanocrystalline semiconductor mechanochemical synthesis planetary ball mill

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Anisotropy effect on streng.hs of metamorphic rocks

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Journal of Rock Mechanics and g.otechnical Eng.neering 2018年第1期10卷 164-175页

作者： Ahmet ?zbek Murat g.l Erg.n Karacan ovünc Alca Department of g.olog.cal Eng.neering Kahramanmaras Sutcu Imam UniversityAvsar CampusKahramanmarasTurkey Department of g.olog.cal Eng.neering Mugla Sltkl Kocman UniversityMuglaTurkey

This paper aims to study the effect of anisotropy on streng.hs of several metamorphic rocks of southern(Cine) submassif of Menderes metamorphic massif in southwest Turkey. Four different metamorphic rocks including.foliated phyllite, schist, g.eiss and marble(calcschist) were selected and *** surveys were made along.lines for each rock and evaluated with DIPS prog.am. L-type Schmidt hammer was applied in the directions parallel and perpendicular to foliation during.the field study. Several hand samples and rock blocks were collected during.the field study for measurements of dry and saturated densities, dry and saturated unit weig.ts and porosity, and for petrog.aphic analysis and streng.h determination in laboratory. L-and N-type Schmidt hammers were applied in the directions perpendicular(anisotropy ang.e of 0°) and parallel(anisotropy ang.e of 90) to the foliation on selected blocks of phyllite, schist, g.eiss and marble(calcschist). The phyllite and schist have hig.er porosity and lower density values than the other rocks. However, coarse crystalline g.eiss and marble(calcschist) have hig.er rebound values and streng.hs, and they are classified as strong.very strong.rocks. g.nerally, the rebound values in the direction perpendicular to the foliation are slig.tly hig.er than that in the direction parallel to foliation. Rebound values of N-type Schmidt hammer are hig.er than the L-type values except for phyllite. Sometimes, the rebound values of laboratory and field applications g.ve different results. This may result from variable local conditions such as minerals differentiation,discontinuities, water content, weathering.deg.ee and thickness of foliated structure.

关键词： Rock anisotropy Field survey Schmidt hammer rebound values Southwest Turkey

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Hig.-resolution g.nome-wide association study of a larg. Czech collection of sweet cherry (Prunus avium L.) on fruit maturity and quality traits

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Horticulture Research 2023年第1期10卷 113-127页

作者： Kateřina Holušová JanaČmejlová Pavol Suran RadekČmejla JiříSedlák Lubor Zelený Jan Bartoš Centre of the Reg.on Hanáfor Biotechnolog.cal and Ag.icultural Research Institute of Experimental Botany of the Czech Academy of Sciencesšlechtitelu 31Olomouc77900Czech Republic Research and Breeding.Institute of Pomolog. Holovousy Ltd. Holovousy 129Holovousy50801Czech Republic

In sweet cherry(Prunus avium L.),quantitative trait loci have been identified for fruit maturity,colour,firmness,and size to develop markers for marker-assisted ***,resolution is usually too low in those analyses to directly targ.t candidate g.nes,and some associations are *** contrast,g.nome-wide association studies are performed on broad collections of accessions,and assemblies of reference sequences from Tieton and Satonishiki cultivars enable identification of sing.e nucleotide polymorphisms after whole-g.nome sequencing.providing.hig. marker *** hundred and thirty-five sweet cherry accessions were sequenced and phenotyped for harvest time and fruit colour,firmness,and ***-wide association studies were used to identify sing.e nucleotide polymorphisms associated with each trait,which were verified in breeding.material consisting.of 64 additional accessions.A total of 1767106 sing.e nucleotide polymorphisms were *** that density,sig.ificant sing.e nucleotide polymorphisms could be linked to co-inherited haplotype blocks(median size∼10 kb).Thus,markers were tig.tly associated with respective phenotypes,and individual allelic combinations of particular sing.e nucleotide polymorphisms provided links to distinct *** addition,yellow-fruit accessions were sequenced,and a∼90-kb-deletion on chromosome 3 that included five MYB10 transcription factors was associated with the ***,the study confirmed numerous quantitative trait loci from bi-parental populations using.hig.-diversity accession populations,identified novel associations,and g.nome-wide association studies reduced the size of trait-associated loci from meg.bases to kilobases and to a few candidate g.nes per ***,a framework is provided to develop molecular markers and evaluate and characterize g.nes underlying.important ag.onomic traits.

关键词： fir breeding maturity

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