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Maternal imprinting of the neonatal microbiota colonization in intrauterine growth restricted piglets:a review

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Journal of Animal Science and Biotechnology 2020年第1期11卷 1-8页

作者： Lili Jiang Cuiping Feng shiyu tao Na Li Bin Zuo Dandan Han Junjun Wang State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Department of Obstetrics and Gynecology China-Japan Friendship HospitalBeijing 100029China.

Early colonization of intestinal microbiota during the neonatal stage plays an important role on the development of intestinal immune system and nutrients absorption of the host.Compared to the normal birth weight(NBW)piglets,intrauterine growth restricted(IUGR)piglets have a different intestinal microbiota during their early life,which is related to maternal imprinting on intestinal microbial succession during gestation,at birth and via suckling.Imbalanced allocation of limited nutrients among fetuses during gestation could be one of the main causes for impaired intestinal development and microbiota colonization in neonatal IUGR piglets.In this review,we summarized the potential impact of maternal imprinting on the colonization of the intestinal microbiota in IUGR piglets,including maternal undernutrition,imbalanced allocation of nutrients among fetuses,as well as vertical microbial transmission from mother to offspring during gestation and lactation.At the same time,we give information about the current maternal nutritional strategies(mainly breastfeeding,probiotics and prebiotics)to help colonization of the advantageous intestinal microbiota for IUGR piglets.

关键词： IUGR piglet Microbial colonization Maternal imprinting Nutritional intervention

KDM4A通过下调BMP9促进乳腺癌细胞MDA-MB-231的迁移和侵袭

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中国癌症杂志 2024年第2期34卷 176-184页

作者：陈远香余涛杨诗雨曾涛魏兰张彦重庆医科大学检验医学院临床检验诊断学教育部重点实验室重庆400016

背景与目的:外源性骨形态发生蛋白9(bone morphogenetic protein 9,BMP9)能抑制人乳腺癌的恶性进展,但其在乳腺癌中常异常低表达。本研究拟探索表观遗传修饰的组蛋白赖氨酸特异性去甲基化酶4A(lysine specific demethylase 4A,KDM4A)在... 详细信息

背景与目的:外源性骨形态发生蛋白9(bone morphogenetic protein 9,BMP9)能抑制人乳腺癌的恶性进展,但其在乳腺癌中常异常低表达。本研究拟探索表观遗传修饰的组蛋白赖氨酸特异性去甲基化酶4A(lysine specific demethylase 4A,KDM4A)在乳腺癌中的表达及作用,探究KDM4A与BMP9之间的关系及其可能的调节机制。方法:通过生物信息学方法分析KDM4A在乳腺癌中的表达及其与BMP9之间的关系,采用实时荧光定量聚合酶链反应(real-time fluorescence quantitative polymerase chain reaction,RTFQ-PCR)和蛋白质印迹法(Western blot)进行验证;采用染色质免疫沉淀(chromatin immunoprecipitation,ChIP)验证KDM4A对BMP9的调控作用,采用RNA稳定性实验及CHX蛋白稳定性实验验证KDM4A对BMP9表达的影响。采用RNA干扰技术及敲低BMP9的腺病毒构建转染KDM4A小干扰RNA(siKDM4A)或感染siBMP9腺病毒(Ad-siBMP9)的外源性重组MDA-MB-231细胞,采用划痕愈合实验、transwell实验分别检测细胞迁移及侵袭能力。结果:生物信息学分析结果表明,KDM4A在乳腺癌中的表达明显高于正常组织,KDM4A与BMP9在乳腺癌中的表达呈负相关关系。RTFQ-PCR及Western blot结果显示,KDM4A在不同乳腺癌细胞系中均高表达,敲低KDM4A可显著上调BMP9。ChIP实验证实,KDM4A可显著富集于BMP9基因启动子区域,降低其组蛋白赖氨酸36号位而不是4号位甲基化水平,从而沉默BMP9表达。RNA稳定性实验及CHX蛋白稳定性实验证实,KDM4A对BMP9的mRNA表达无明显影响,但可影响其蛋白降解。敲低KDM4A后,乳腺癌细胞MDA-MB-231的迁移及侵袭能力均受到明显抑制,而这种作用可被敲低BMP9所部分逆转。结论:KDM4A在乳腺癌组织及乳腺癌细胞MDA-MB-231中高表达,并可通过下调BMP9基因启动子区域组蛋白甲基化水平沉默其表达,以及在蛋白水平而不是mRNA水平影响BMP9稳定性,促进乳腺癌的迁移和侵袭。

关键词：乳腺癌赖氨酸特异性去甲基化酶4A 组蛋白去甲基化骨形态发生蛋白9

Comparative proteomic analysis of the effects of high-concentrate diet on the hepatic metabolism and inflammatory response in lactating dairy goats

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Journal of Animal Science and Biotechnology 2016年第2期7卷 217-227页

作者： Yongqian Duanmu Rihua Cong shiyu tao Jing Tian Haibo Dong Yuanshu Zhang Yingdong Ni Ruqian Zhao Key Laboratory of Animal Physiology & Biochemistry Ministry of AgricultureNanjing Agricultural University College of Veterinary Medicine Northwest A & F University

Background： To understand the impact of feeding a high-concentrate diet to mid-lactating goats for a long time on liver metabolism and inflammatory response, two dimensional polyacrylamide gel electrophoresis（2-DE） and real-time PCR method were employed to detect proteins differentially expressed in liver and their m RNAs expression in goats fed high concentrate diet（HC） or low concentrate diet（LC）. Twelve lactating dairy goats were randomly assigned to either a HC diet group（65 % concentrate of dry matter; n = 6） or a LC diet group（35 % concentrate of dry matter; n = 6） for 10 wk.Results： Twenty differentially expressed spots（≥2.0-fold changes） in the hepatic tissues were excised and successfully identified using MALDI TOF/TOF. Of these, 8 proteins were up-regulated, while the rest 12 proteins were down-regulated in HC goats compared to LC. Differential expressed proteins including alpha enolase 1（ENO1）, glutamate dehydrogenase 1（GLUD1）, glutathione S-transferase A1（GSTA1）, ATP synthase subunit 5β（ATP5β）, superoxide dismutase [Cu-Zn]（SOD1）, cytochrom c oxidase subunit Via（COX6A1） and heat shock protein 60（HSP60） were further verified by real-time PCR and/or western blot at m RNA or protein expression level. Consistent with the 2-DE results, a significant decrease of β-actin protein expression and SOD enzyme activity was observed in liver of HC goats（P 〈 0.05）, while ENO1 protein expression was significantly up-regulated in HC compared to LC goats（P 〈 0.05）. However, western blot analysis did not show a significant difference of hepatic HSP60 protein between HC and LC group, which did not match the decrease of HSP60 content detected by 2-DE analysis. Real-time PCR showed that glutathione S-transferase P1（GSTP1） and SOD1 m RNA expression was significantly decreased in liver of HC goats, while cytochrom c oxidase（COX3） and ATPase 8（ATP8） m RNAs expression were markedly increased compared to LC（P 〈 0.05）. Gene Ontology（GO） analysis revealed that HC diet resulted in altered expression of proteins related to catalytic

关键词： High concentrate diet Lactating goats Liver Mitochondria Proteomics

Effects of chronic dexamethasone administration on hyperglycemia and insulin release in goats

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Journal of Animal Science and Biotechnology 2018年第3期9卷 585-594页

作者： Liqiong Niu Qu Chen Canfeng Hua Yali Geng Liuping Cai shiyu tao Yingdong Ni Ruqian Zhao Key Laboratory of Animal Physiology & Biochemistry Nanjing Agricultural University

Background: Dexamethasone(Dex), a synthetic glucocorticoid, is among the most commonly used drugs worldwide in animals and humans as an anti-inflammatory and immunosuppressive agent. GC has profound effects on plasma glucose level and other metabolic conditions. However, the effect of prolonged use of Dex on glucose metabolism in ruminants is still unclear.Results: Ten goats were randomly assigned to two groups: the control goats were injected with saline, and the Dex-treated goats were intramuscularly injected daily for 21 d with 0.2 mg/kg Dex. The results showed that plasma glucose and insulin concentrations were significantly increased after Dex administration(P 0.05). The expression of several key genes, involved in blood glucose regulation, was detected by real-time PCR in the small intestine, skeletal muscle and liver. The expression of glucose transporter type 2(GLUT2), sodium-glucose transporter 1(SGLT1) and sodium-potassium ATPase(Na-K/ATPase) in the small intestine were generally increased by Dex, and GLUT2 m RNA expression was significantly up-regulated(P < 0.05). In liver, the expression of genes involved in gluconeogenesis including glucose-6-phosphatase catalytic subunit(G6 PC), cytosolic form of phosphoenolpyruvate carboxykinase(PCK1) and pyruvate carboxylase(PC), were significantly down-regulated by Dex. However, the protein expression levels of PCK1 & PCK2 were significantly increased by Dex, suggesting a post-transcriptional regulation. In dorsal longissimus, the m RNA expression of genes associated with gluconeogenesis and the insulin signaling pathway were generally upregulated by Dex, but the m RNA expression of two markers of muscle atrophy, namely F-box protein 32(FBXO32/Atrogin1) and muscle RING-finger protein 1(Mu RF1), was not altered by Dex.Conclusions: Taken together, these results indicate that c

关键词： Blood glucose Dexamethasone Gluconeogenesis Goat Liver

Negative effects of long-term feeding of high-grain diets to lactating goats on milk fat production and composition by regulating gene expression and DNA methylation in the mammary gland

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Journal of Animal Science and Biotechnology 2018年第1期9卷 217-227页

作者： Ping Tian Yanwen Luo Xian Li Jing Tian shiyu tao Canfeng Hua Yali Geng Yingdong Ni Ruqian Zhao Key Laboratory of Animal Physiology&Biochemistry Nanjing Agricultural University College of Veterinary Medicine Northwest A and F University

Background： It is well known that feeding a high concentrate（HC） diet to lactating ruminants likely induces subacute ruminal acidosis（SARA） and leads to a decrease in milk fat production. However, the effects of feeding a HC diet for long periods on milk fatty acids composition and the mechanism behind the decline of milk fat still remains poorly understood. The aim of this study was to investigate the impact of feeding a HC diet to lactating dairy goats on milk fat yield and fatty acids composition with an emphasis on the mechanisms underlying the milk fat depression. Seventeen mid-lactating dairy goats were randomly allocated to three groups. The control treatment was fed a low-concentrate diet（35% concentrate, n = 5, LC） and there were two high-concentrate treatments（65% concentrate, HC）, one fed a high concentrate diet for a long period（19 wks, n = 7, HL）; one fed a high concentrate diet for a short period of time（4 wk, n = 5, HS）. Milk fat production and fatty acids profiles were measured. In order to investigate the mechanisms underlying the changes in milk fat production and composition,the gene expression involved in lipid metabolism and DNA methylation in the mammary gland were also analyzed.Results： Milk production was increased by feeding the HC diet in the HS and HL groups compared with the LC diet（P 〈 0.01）, while the percentage of milk fat was lower in the HL（P 〈 0.05） but not in the HS group. The total amount of saturated fatty acids（SFA） in the milk was not changed by feeding the HC diet, whereas the levels of unsaturated fatty acids（UFA） and monounsaturated fatty acids（MUFA） were markedly decreased in the HL group compared with the LC group（P 〈 0.05）. Among these fatty acids, the concentrations of C15：0（P 〈 0.01）, C17：0（P 〈 0.01）, C17：1（P 〈 0.01）, C18：1 n-9 c（P 〈 0.05）, C18：3 n-3 r（P 〈 0.01） and C20：0（P 〈 0.01） were markedly lower in the HL group, and the concentrations of C20：0（P 〈 0.05） and C18：3 n-3 r（P 〈 0.01） were lower in the HS group compared with the LC group. However, the concentrations

关键词： DNA methylation Gene expression Goat High concentrate diet Milk fat

Early life administration of milk fat globule membrane promoted SCFA-producing bacteria colonization,intestinal barriers and growth performance of neonatal piglets

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Animal Nutrition 2021年第2期7卷 346-355页

作者： Yujun Wu Xiangyu Zhang Dandan Han Yu Pi shiyu tao Shiyi Zhang Shilan Wang Junying Zhao Lijun Chen Junjun Wang State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing100193China National Engineering Center of Dairy for Early Life Health Beijing Sanyuan Foods Co.LtdBeijing100163China

Milk fat globule membrane(MFGM)possesses various nutritional and biological benefits for mammals,whereas its effects on neonatal gut microbiota and barrier integrity remained unclear.This study investigated the effects of MFGM administration on microbial compositions and intestinal barrier functions of neonatal piglets.Sixteen newborn piglets were randomly allocated into a CON group or MFGM group,orally administered with saline or MFGM solution(1 g/kg body weight)respectively during the first postnatal week,and all piglets were breastfed during the whole neonatal period.The present study found that the MFGM oral administration during the first postnatal week increased the plasma immunoglobulin(lg)G level,body weight and average daily gain of piglets(P<0.05)on 21 d.Addi-tionally,MFGM administration enriched fecal SCFA-producing bacteria(Ruminococaceae_UCG-002,Ruminococaceae_UCG-010,Ruminococaceae_UCG-004,Ruminococaceae_UCG-014 and[Ruminococcus]_gauvrearuii_group),SCFA concentrations(acetate,propionate and butyrate;P<0.05)and their receptor(G-protein coupled receptor 41,GPR41).Furthermore,MFGM administration promoted intestinal villus morphology(P<0.05)and barrier functions by upregulating genes of tight junctions(E-cadherin,claudin-1,occludin and zonula occludin 1[ZO-1]),mucins(mucin-13 and mucin-20)and interleukin(IL)-22(P<0.05).Positive correlation was found between the beneficial microbes and SCFA levels pairwise with the intestinal barrier genes(P<0.05).In conclusion,orally administrating MFGM during the first postnatal week stimulated SCFA-producing bacteria colonization and SCFA generation,enhanced intes-tinal barrier functions and consequently improved growth performance of neonatal piglets on 21 d.Our findings will provide new insights about MFGM intervention for microbial colonization and intestinal development of neonates during their early life.

关键词： Milk fat globule membrane Gut microbiota SCFA Intestinal barrier Piglet

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“从阴引阳,从阳引阴”在药物应用中之体会

四川中医 2024年第4期42卷 53-55页

作者：范诗宇于涛天津中医药大学第一附属医院/国家中医针灸临床医学研究中心天津300380

自《素问·阴阳应象大论》提出“从阴引阳,从阳引阴”的概念后,后世医家多运用此理论以指导针刺治疗由阴阳失和引起的一系列病证。而张介宾独出心裁提出“从阴引阳,从阳引阴”亦为用药之理,并举出“求汗于血,生气于精”、“引火归元,纳... 详细信息

自《素问·阴阳应象大论》提出“从阴引阳,从阳引阴”的概念后,后世医家多运用此理论以指导针刺治疗由阴阳失和引起的一系列病证。而张介宾独出心裁提出“从阴引阳,从阳引阴”亦为用药之理,并举出“求汗于血,生气于精”、“引火归元,纳气归肾”等法则,今仅就张介宾“从阴引阳,从阳引阴”中用药之理浅谈个人体会。

关键词：从阴引阳从阳引阴用药之理张介宾

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基于自由曲面光学元件的大视场光学成像系统

光学精密工程 2024年第9期32卷 1261-1272页

作者：熊玉朋陈适宇黄铖路文文彭小强陈善勇赖涛欧洋国防科技大学智能科学学院装备综合保障技术重点实验室湖南长沙410073 超精密加工技术湖南省重点实验室湖南长沙410073 国防科技大学南湖之光实验室湖南长沙410073

面向机载探测系统大视场光学成像的需求,开展了大视场光学成像系统光学设计、自由曲面光学元件超精密加工、形位误差同步检测以及系统集成与成像实验研究。首先,采用视场扩展法进行大视场自由曲面离轴反射光学系统的设计;其次,进行铝合... 详细信息

面向机载探测系统大视场光学成像的需求,开展了大视场光学成像系统光学设计、自由曲面光学元件超精密加工、形位误差同步检测以及系统集成与成像实验研究。首先,采用视场扩展法进行大视场自由曲面离轴反射光学系统的设计;其次,进行铝合金自由曲面反射镜纳米精度加工和高频抑制工艺探索,并实现了基于计算全息元件的自由曲面形位高精度检测;最后,进行了光学系统的装调集成与成像实验。结果表明,系统的视场角为30°×5°,全视场光学传递函数值大于0.7,接近衍射极限,最大像元均方根半径为2.075μm,自由曲面光学元件的面形精度均方根(Root Mean Square,RMS)值优于20 nm,位置精度优于1μm,装配集成后能够满足大视场高分辨的场景使用要求,同时具备稳定可靠和快响制造等特点。

关键词：光学超精密加工自由曲面计算全息补偿检测成像系统

Intrauterine growth restriction alters nutrient metabolism in the intestine of porcine offspring

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Journal of Animal Science and Biotechnology 2021年第2期12卷 649-660页

作者： Tiantian Li Shimeng Huang Long Lei shiyu tao Yi Xiong Guoyao Wu Jie Hu Xiongkun Yuan Shengjun Zhao Bin Zuo Hongjian Yang Yingping Xiao Gang Lin Junjun Wang State Key Laboratory of Animal Nutrition College of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193People’s Republic of China Hubei Collaborative Innovation Center for Animal Nutrition and Feed Safety Hubei Key Laboratory of Animal Nutrition and Feed ScienceWuhan Polytechnic UniversityWuhan 430023People’s Republic of China Department of Animal Science Texas A&M UniversityCollege StationTX 77843USA State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products Institute of Quality and Standard for Agro-productsZhejiang Academy of Agricultural SciencesHangzhou 310021People’s Republic of China Key Laboratory of Agrifood Safety and Quality Institute of Quality Standards and Testing Technology for Agricultural ProductsChinese Academy of Agricultural SciencesMinistry of Agriculture and Rural AffairsBeijing 100081People’s Republic of China

Background:Intrauterine growth restriction(IUGR)has negative impacts on the postnatal survival,growth and development of humans and animals,with not only on newborns but also adulthood.However,the characteristics for nutrient digestion and absorption in IUGR offspring are still largely unknown.Therefore,the normal birth weight(NBW)and IUGR growing pigs were used in this study to investigate their differences in nutrient utilization,with an expectition for further nutritional optimization of the IUGR offspring during their later life.Methods:Twelve IUGR and 12 NBW growing pigs were fitted with catheters in their portal vein to measure blood flow rate as well as nutrients and metabolites in plasma.The digestibilities of nutrients in different intestinal segments,and bacterial fermentation in the large intestine were examined to reveal the characteristics of nutrients utilization in IUGR versus NBW pigs.Results:The rate of portal venous blood flow did not differ beween IUGR and NBW pigs.Plasma concentrations of total cholesterol,triglycerides and glucose were much lower but those of urea were higher in the portal vein of IUGR pigs,compared with the NBW pigs.The ileal digestibility of dry matter,gross energy and starch were lower in IUGR pigs than in NBW pigs.IUGR increased hindgut microbial diversity and bacterial fermentation activity in the caecum.In vitro cross-fermentation of ileal digesta by caecal microbes of NBW and IUGR pigs showed that gas production was much higher for IUGR ileal digesta regardless of the source of caecal inocula.Conclusion:IUGR impairs the nutrient digestion and absorption in small intestine,reduces caecal microbial diversity and promotes bacterial fermentation in the large intestine during the growing phase.These findings aid in our understanding of nutrient metabolism in IUGR pigs and provide the basis for future nutritional interventions.

关键词： Absorption Digestion Fermentation IUGR pigs Portal vein