检索结果-南通市图书馆

小麦肽及其与大豆肽、豌豆肽复配肽的体外组合对TNF-α刺激C2C12骨骼肌细胞蛋白表达的影响

在线全文

学校读者我要写书评

暂无评论

食品与发酵工业 2024年第2期50卷 153-158页

作者：程改平李明亮刘婧徐淼李可闫九明任玮秦修远四川大学华西医院四川成都610041 江南大学粮食发酵与食品生物制造国家工程研究中心江苏无锡214122 中国食品发酵工业研究院有限公司北京100015 北京市蛋白功能肽工程技术研究中心北京100015

通过细胞实验探讨小麦肽、大豆肽、豌豆肽复合物对TNF-α刺激C2C12骨骼肌细胞蛋白表达的影响,并初步探明作用通路。采用终质量浓度为25 ng/mL的TNF-α处理C2C12细胞4 d建立TNF-α刺激模型,CCK-8法确定小麦肽、大豆肽、豌豆肽对C1C12细... 详细信息

通过细胞实验探讨小麦肽、大豆肽、豌豆肽复合物对TNF-α刺激C2C12骨骼肌细胞蛋白表达的影响,并初步探明作用通路。采用终质量浓度为25 ng/mL的TNF-α处理C2C12细胞4 d建立TNF-α刺激模型,CCK-8法确定小麦肽、大豆肽、豌豆肽对C1C12细胞的安全剂量;检测不同复配比例的小麦肽、大豆肽、豌豆肽实验组的肌球蛋白相关指标(MyoD、MyoG、MyHC)、泛素-蛋白酶体系统肌肉萎缩相关指标(TRIM63和MAFbx)的蛋白表达、Akt、mTOR和其磷酸化蛋白表达情况。结果表明,与对照组相比,TNF-α刺激的C2C12细胞中MyoD、MyoG和MyHC的蛋白表达量均有下降,其中MyHC显著降低;MAFbx和TRIM63表达量显著升高;Akt和mTOR的磷酸化水平显著降低,而经过肽样品处理后,各指标均有缓解,且以小麦肽与大豆肽+豌豆肽质量比1∶3效果最好。小麦肽与大豆肽+豌豆肽以1∶3的质量比进行复配在几种配比中可以最有效地改善由TNF-α造成的C2C12细胞肌蛋白含量降低,可通过调节E3泛素连接酶的表达和提高Akt/mTOR的蛋白磷酸化水平的通路,从而降低细胞中蛋白降解和促进蛋白合成。

关键词：小麦肽大豆肽豌豆肽 C2C12骨骼肌细胞肌肉萎缩 Akt/mTOR

Numerical Modelling of Electroosmotic Driven Flow in Nanoporous Media by Lattice Boltzmann Method

在线全文

学校读者我要写书评

暂无评论

Journal of Bionic Engineering 2013年第1期10卷 90-99页

作者： Bo Li Wenning Zhou Yuying Yan Zhiwu Han Luquan ren Energy and Sustainability Research Division Faculty of Engineering University of Nottingham Nottingham NG7 2RD UK Key Laboratory of Bionic Engineering (Ministry of Education China) Jilin University Changchun 130022 P. R. China

The lattice Boltzmann method was employed to simulate electroosmotic driven flow and Debye layer screening in con- ducting electrolyte around a porous structure with average size of 40 nm. The charge screening around the nanopores was investigated by solving the vector-superpositioned potential equilibrium distribution function and adding electro-kinetic force term to the evolution equation. In this intermediate case of moderate Debye length, the electrophoresis problem becomes complicated. The motion of the particles distorts the screening cloud, which becomes asymmetric, resulting in very complex interactions between the electrolyte, the screening cloud and the particle; but the Electroosmotic Flow （EOF） behaviour was still considered based on the Helmoholtz-Smoluchowski model with adaptation to fit nanoporous flow in the porous structure. In the present approach, the flow in the nanopores is directly modelled; the detailed flow information can be obtained by simplifying the repeated macrostructure. Due to the symmetry of the domain, the size of computational domain can be largely reduced by less repeated spherical nanoparticles. Each pore of the medium contains several lattice nodes on the simplified curved edges and potential gradients are produced by adjusting the zeta potential value. The velocity results for pressure-driven and EOF flows agree well with the analytical solutions and recent experimental results. In particular, the interface between solid particles and fluids, the influences of porosity, solid particle diameter, yield stress and electric parameters in EOF were investigated. The anti-adhesion effect of electroosmotic pumping effect was evaluated, and the pulsed DC was applied in order to enhance the performance of the electroosmotic pumping. The results demonstrate that the present lattice Boltzmann model is capable of modelling flow through nanoporous media at certain restrictions while some results deviate from the predictions based on the macr

关键词： electroosmotic flow electroosmotic pumping nanoporous media lattice Boltzmann method numerical modelling,anti-adhesion

Precise control of graphene etching by remote hydrogen plasma

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Nano Research 2019年第1期12卷 137-142页

作者： Bangjun Ma Shizhao ren Peiqi Wang Chuancheng Jia Xuefeng Guo Beijing National Laboratory for Molecular Sciences State Key Laboratory for Structural Chemistry of Unstable and Stable SpeciesCollege of Chemistry and Molecular EngineeringPeking UniversityBeijing 100871China Department of Materials Science and Engineering Peking UniversityBeijing 100871China

Graphene with atomically smooth and configuration-specific edges plays the key role in the performance of graphene-based electronic *** hydrogen plasma etching of graphene has been proven to be an effective way to create smooth edges with a specific zigzag ***,the etching process is still poorly *** this study,with the aid of a custom-made plasma-enhanced hydrogen etching(PEHE)system,a detailed graphene etching process by remote hydrogen plasma is ***,we find that hydrogen plasma etching of graphene shows strong thickness and temperature *** etching process of single-layer graphene is *** is opposite to the an isotropic etchi ng effect observed for bilayer and thicker graphe ne with an obvious depe ndence on *** the basis of these observations,a geometrical model was built to illustrate the configuration evolution of graphene edges during etching,which reveals the origi n of the an isotropic etchi ng *** further utilizi ng this model,armchair graphe ne edges were also prepared in a con trolled manner for the first *** investigations offer a better understanding of the etching process for graphene,which should facilitate the fabricati on of graphe ne?based electr onic devices with con trolled edges and the explorati on of more in teresti ng properties of graphe ne.

关键词： graphene hydrogen plasma anisotropic etching electronic device

Nanostructured CdS for efficient photocatalytic H2 evolution: A review

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Materials 2020年第11期63卷 2153-2188页

作者： Rongchen Shen Doudou ren Yingna Ding Yatong Guan Yun Hau ng Peng Zhang Xin Li Key Laboratory of Energy Plants Resource and Utilization Ministry of Agriculture and Rural AffairsCollege of Forestry and Landscape ArchitectureSouth China Agricultural UniversityGuangzhou 510642China College of Materials and Energy South China Agricultural UniversityGuangzhou 510642China School of Energy and Environment City University of Hong KongHongKong SARChina State Center for International Cooperation on Designer Low-Carbon&Environmental Materials School of Materials Science and EngineeringZhengzhou UniversityZhengzhou 450001China

Cadmium sulfide(Cd S)-based photocatalysts have attracted extensive attention owing to their strong visible light absorption,suitable band energy levels,and excellent electronic charge transportation *** review focuses on the recent progress related to the design,modification,and construction of Cd S-based photocatalysts with excellent photocatalytic H2 evolution ***,the basic concepts and mechanisms of photocatalytic H2 evolution are briefly ***,the fundamental properties,important advancements,and bottlenecks of Cd S in photocatalytic H2 generation are presented in detail to provide an overview of the potential of this ***,various modification strategies adopted for Cd S-based photocatalysts to yield solar H2 are discussed,among which the effective approaches aim at generating more charge carriers,promoting efficient charge separation,boosting interfacial charge transfer,accelerating charge utilization,and suppressing charge-induced *** critical factors governing the performance of the photocatalyst and the feasibility of each modification strategy toward shaping future research directions are comprehensively discussed with ***,the prospects and challenges encountered in developing nanostructured Cd S and Cd S-based nanocomposites in photocatalytic H2 evolution are presented.

关键词： solar fuel nanostructured cadmium sulfide-based photocatalysts modification strategies hydrogen production photocharge utilization

3-甲基腺嘌呤通过抑制AKT信号减轻糖尿病小鼠的早期肾损伤

在线全文

学校读者我要写书评

暂无评论

南方医科大学学报 2024年第7期44卷 1236-1242页

作者：刘本菊王业磊任海文欧丽雯邓轩黄梦欣吴鑫龚权长江大学医学部人体解剖学教研室湖北荆州434023 长江大学医学部免疫学教研室湖北荆州434023 重庆市彭水自治县人民医院内科重庆彭水409600 重庆医科大学附属璧山医院检验科重庆璧山402760

目的探究3-甲基腺嘌呤(3-MA)减轻糖尿病早期肾损伤的机制。方法将24只小鼠随机分为对照组(CON组,n=8)和模型组(n=16)。模型组采用腹腔注射链脲佐菌素(STZ)诱导糖尿病,CON组注射等体积柠檬酸缓冲液。将造模成功的小鼠随机分为糖尿病组(DM... 详细信息

目的探究3-甲基腺嘌呤(3-MA)减轻糖尿病早期肾损伤的机制。方法将24只小鼠随机分为对照组(CON组,n=8)和模型组(n=16)。模型组采用腹腔注射链脲佐菌素(STZ)诱导糖尿病,CON组注射等体积柠檬酸缓冲液。将造模成功的小鼠随机分为糖尿病组(DM组,n=8)和3-MA干预糖尿病组(DM+3-MA组,n=8),分别灌胃给予等体积生理盐水和3-MA 10 mg/(kg·d),每周记录小鼠体质量和空腹血糖,干预6周后取肾,记录肾/体质量比,PAS染色观察肾小球大小,Western blotting和免疫组化法检测肾皮质α-平滑肌肌动蛋白(α-SMA)和增殖细胞核抗原(PCNA)蛋白表达。将SV40 MES 13细胞分别采用5.6 mmol/L正常糖(ng组)、5.6 mmol/L正常糖+24.4 mmol/L甘露醇(ng+M组)、30 mmol/L高糖(HG组)和30 mmol/L高糖加5 mmol/L 3-MA(HG+3-MA组)培养24 h,CCK8法检测细胞活力,Western blotting检测PCNA蛋白表达。对糖尿病肾脏疾病(DKD)基因靶点与3-MA预测基因靶点的交集基因进行生信分析,并外采用Western blotting对生信分析结果进行验证。结果体内实验结果显示,3-MA具有短期降血糖作用,可减轻糖尿病小鼠肾/体质量比和肾小球肥大(均Png结果显示,3-MA在体内外均可抑制AKT及核糖体蛋白S6(S6)的磷酸化。结论3-MA通过抑制AKT信号减少系膜细胞增殖减轻糖尿病早期肾损伤。

关键词： 3-甲基腺嘌呤糖尿病肾脏疾病系膜细胞肾损伤

益糖康抑制坏死性凋亡及EMT减轻糖尿病肾病肾损伤的实验研究

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

时珍国医国药 2024年第9期35卷 2151-2155页

作者：孟桐宇杨宇峰任艳玲石岩辽宁中医药大学辽宁沈阳110847

目的探究益糖康对糖尿病肾病肾损伤的影响,并研究其对上皮-间质转化和坏死性凋亡相关途径的作用及机制。方法细胞分为ng组、HG组、HG+NEC-1组、HG+YTK组、HG+DAPA组。动物分为正常对照组、模型组、阳性对照组、益糖康(YTK)高、中、低剂... 详细信息

目的探究益糖康对糖尿病肾病肾损伤的影响,并研究其对上皮-间质转化和坏死性凋亡相关途径的作用及机制。方法细胞分为ng组、HG组、HG+NEC-1组、HG+YTK组、HG+DAPA组。动物分为正常对照组、模型组、阳性对照组、益糖康(YTK)高、中、低剂量组。通过CCK-8、流式细胞术检测细胞增殖活力以及凋亡水平;通过HE、Masson染色以及透射电镜观察肾组织的形态和超微结构;通过Western blot检测细胞及肾组织中坏死性凋亡以及EMT相关蛋白的表达。结果经益糖康治疗后,HK-2细胞增殖活力增强,凋亡率降低(P<0.05)。在体内外实验中,坏死性凋亡以及EMT相关蛋白RIP1、RIP3、MLKL、Vimentin、α-SMA水平下降,E-cadherin水平升高(P<0.05)。结论YTK能够减轻糖尿病肾病引起的肾损伤,其作用机制可能与抑制坏死性凋亡和EMT的发生有关。

关键词：糖尿病肾病益糖康坏死性凋亡上皮-间质转化肾损伤

一种适用于梅花鹿源性产品鉴定的微量组织DNA提取方法的建立

在线全文

学校读者我要写书评

暂无评论

特产研究 2024年第5期46卷 54-57页

作者：任雨贺金美伶宋超中国农业科学院特产研究所吉林长春130112

为建立一种适宜梅花鹿源性产品分子鉴定的高效、便捷微量组织基因组DNA提取的方法。本研究分别以鲜品梅花鹿茸、风干梅花鹿茸、烘干梅花鹿茸、鲜品梅花鹿鞭、烘干梅花鹿鞭、风干梅花鹿鞭、鲜品梅花鹿肉、干品梅花鹿尾、梅花鹿血为样本,... 详细信息

为建立一种适宜梅花鹿源性产品分子鉴定的高效、便捷微量组织基因组DNA提取的方法。本研究分别以鲜品梅花鹿茸、风干梅花鹿茸、烘干梅花鹿茸、鲜品梅花鹿鞭、烘干梅花鹿鞭、风干梅花鹿鞭、鲜品梅花鹿肉、干品梅花鹿尾、梅花鹿血为样本,采用Chelex-100树脂溶液和蛋白酶K结合的方法提取基因组DNA,并检测其浓度与纯度;以此DNA为模板,扩增梅花鹿线粒体DNA的Dloop区特异性基因,并对PCR扩增产物进行琼脂糖核酸电泳及测序。结果表明,基于Chelex-100树脂提取法获得的基因组DNA模板浓度为52.57~63.39 ng/μL,A260/A280比值为1.75~1.96;梅花鹿线粒体特异性基因扩增产物电泳条带清晰,且长度准确;扩增产物测序,相似性大于95%。Chelex-100树脂提取法能够提取微量梅花鹿组织样本基因组DNA,且此方法低价、简便,适用于梅花鹿源性产品的真伪鉴定。

关键词：梅花鹿 Chelex-100树脂提取法 DNA提取源性产品鉴定

血清miR-599联合VEGF预测分化型甲状腺癌患者次全切除术后复发的价值

同方期刊数据库博看期刊评论

在线全文

学校读者我要写书评

暂无评论

国际医药卫生导报 2024年第13期30卷 2219-2224页

作者：杨凯王斌锋任彦顺惠鹏铜川市人民医院急诊外科铜川727000 铜川市人民医院普外科铜川727000 安康市中心医院普外科安康725000

目的分析血清微RNA-599(miR-599)联合血管内皮生长因子(VEGF)水平对分化型甲状腺癌(DTC)患者全腔镜甲状腺次全切除术后复发的预测效能。方法选取铜川市人民医院2017年1月至2022年3月收治的行全腔镜甲状腺次全切除术的DTC患者135例。患... 详细信息

目的分析血清微RNA-599(miR-599)联合血管内皮生长因子(VEGF)水平对分化型甲状腺癌(DTC)患者全腔镜甲状腺次全切除术后复发的预测效能。方法选取铜川市人民医院2017年1月至2022年3月收治的行全腔镜甲状腺次全切除术的DTC患者135例。患者术前均检测血清miR-599和VEGF水平,并收集患者术前临床相关资料。术后对患者进行为期2年的随访,根据术后复发情况将其分为复发组(31例)和无复发组(104例)。复发组男12例,女19例,年龄(51.26±7.32)岁;无复发组男48例,女56例,年龄(49.57±7.08)岁。计数资料采用χ^(2)检验或Fisher精确概率检验;计量资料采用独立样本t检验。患者术后复发的影响因素用多因素逐步logistic回归模型进行分析;血清miR-599联合VEGF水平预测患者术后复发的效能用受试者操作特征曲线(ROC)下面积(AUC)进行分析。结果复发组miR-599水平低于无复发组[(0.81±0.09)比(1.05±0.12)],VEGF水平高于无复发组[(32.55±5.26)ng/L比(24.83±4.07)ng/L](t=10.295、8.639,均P<0.001)。复发组Ⅲ~Ⅳ期占比[67.74%(21/31)比28.85%(30/104)]、抗甲状腺球蛋白抗体(TgAb)水平[(251.86±27.98)IU/ml比(211.43±23.49)IU/ml]高于无复发组,术中淋巴结清扫占比[22.58%(7/31)比44.23%(46/104)]、术后放射碘治疗占比[32.26%(10/31)比53.85%(56/104)]低于无复发组(t=8.040,χ^(2)=4.694、4.454,均P<0.05)。多因素逐步logistic回归分析显示,miR-599水平(OR=0.251,95%CI 0.109~0.576)是患者术后复发的独立保护因素,VEGF水平(OR=2.163,95%CI 1.256~3.722)、临床分期(OR=4.287,95%CI 1.908~9.631)、TgAb水平(OR=4.612,95%CI 2.172~9.789)是患者术后复发的独立危险因素(均P<0.05)。ROC显示,miR-599、VEGF及二者联合预测患者术后复发的灵敏度分别为71.00%、74.20%、90.30%,特异度分别为76.90%、72.10%、88.50%,AUC分别为0.824、0.794、0.931(均P<0.05)。结论血清VEGF水平是DTC患者全腔镜甲状腺次全切除术后复发的独立危险因素,血清miR-599水平是患者术后复发的独立保护因素;二者联合检测可较好地预测患者术后复发风险。

关键词：分化型甲状腺癌微RNA-599 血管内皮生长因子全腔镜甲状腺次全切除术术后复发预测效能

小儿消积止咳口服液及药用复合膜中12种常见抗氧剂的提取与迁移研究

在线全文

学校读者我要写书评

暂无评论

分析测试学报 2024年第5期43卷 763-769页

作者：成婕蒲小聪邱怡婷任锟杨剑郭燕燕王晓兰江燕四川省药品检验研究院工业和信息化部产业技术基础公共服务平台四川成都611731

建立了测定小儿消积止咳口服液及其包装材料(口服液体药用复合膜)中12种常见抗氧剂的超高效液相色谱-串联质谱(UPLC-MS/MS)法。采用Shim-pack GIST C18(100 mm×2.1 mm,2μm)为色谱柱,甲醇(含0.01 mol/L甲酸与甲酸铵)和水(含0.01 mol/L... 详细信息

建立了测定小儿消积止咳口服液及其包装材料(口服液体药用复合膜)中12种常见抗氧剂的超高效液相色谱-串联质谱(UPLC-MS/MS)法。采用Shim-pack GIST C18(100 mm×2.1 mm,2μm)为色谱柱,甲醇(含0.01 mol/L甲酸与甲酸铵)和水(含0.01 mol/L甲酸与甲酸铵)为流动相梯度洗脱;采用电喷雾离子源(ESI),以多反应监测(MRM)正离子模式进行定性定量分析。结果显示,12种抗氧剂在10~400 ng/mL范围内线性良好,相关系数(r)不小于0.9963,提取与迁移实验的检出限分别为0.40~13.25 ng/g和1.01~33.13 ng/mL,定量下限分别为1.35~44.17 ng/g和3.37~110.42 ng/mL,回收率为80.1%~114%,相对标准偏差(RSD,n=3)为1.5%~7.2%。药液中检出抗氧剂1010和168,但含量低于其每日允许最大暴露量(PDE),对药品安全性风险影响较小。该方法高效灵敏、准确可靠、操作简便,可用于小儿消积止咳口服液及其包装材料中12种常见抗氧剂的测定。

关键词：超高效液相色谱-串联质谱(UPLC-MS/MS) 抗氧剂小儿消积止咳口服液药用复合膜